The effect of rumen ciliates on chitinolytic activity, chitin content and the number of fungal zoospores in the rumen fluid of sheep

References

• Ankrah P, Loerch SC, Dehority BA. 1990. Sequestration migration and lysis of protozoa in the rumen. J Gen Microbiol. 136:1869–1875.
   Google Scholar
• [AOAC] Association of Official Analytical Chemists. 2000. Official methods of analysis of AOAC. 17th ed. Washington (DC): Association of Official Analytical Chemists.
   Google Scholar
• Bauchop T. 1989. Colonization of plant fragments by protozoa and fungi. In: Nolan JV, Leng RA, Demeyer DI, editors. The roles of protozoa and fungi in ruminant digestion. Proceedings of an International Seminar held at the University of New England; 1988 Sep 26–29; Armidale. Armidale: Prenambul Books Armidale; p. 83–96.
   Google Scholar
• Bełżecki G, Miltko R, Kwiatkowska E, Kowalik B, Michałowski T. 2012. The effect of composition of ciliate fauna on murein content and mureinolytic activity in the rumen of sheep. J Anim Feed Sci. 21:65–76.
   Web of Science ®Google Scholar
• Bełżecki G, Miltko R, Michałowski T, McEwan NR. 2016. Methods for the cultivation of ciliated protozoa from the large intestine of horses. FEMS Microbiol Lett. 363:fnv233.
   PubMed Web of Science ®Google Scholar
• Bełżecki G, Miltko R, Michałowski T, Šimůnek J, Kopečný J. 2008. Chitinolytic activity of the sheep rumen ciliate Diploplastron affine. Folia Microbiol. 53:201–203.
   PubMed Web of Science ®Google Scholar
• Bonhomme A. 1990. Rumen ciliates: their metabolism and relationships with bacteria and their hosts. Anim Feed Sci Technol. 30:203–266.
   Web of Science ®Google Scholar
• Chen GC, Johnson BR. 1983. Improved colorimetric determination of cell wall chitin in wood decay fungi. Appl Environ Microbiol. 46:13–16.
   PubMed Web of Science ®Google Scholar
• Clarke RTJ. 1965. Diurnal variation in the numbers of rumen ciliate protozoa in cattle. New Zeal J Agr Res. 8:1–9.
   Google Scholar
• Coleman G, Davies JI, Cash MA. 1972. The cultivation of the rumen ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro. J Gen Microbiol. 73:509–521.
   PubMedGoogle Scholar
• Davies DR, Theodorou MK, Lawrence MI, Trinci AP. 1993. Distribution of anaerobic fungi in the digestive tract of cattle and their survival in faeces. J Gen Microbiol. 139:1395–1400.
   PubMedGoogle Scholar
• Dehority BA. 2003. Rumen microbiology. Nottingham: Nottingham University Press.
   Google Scholar
• Denman SE, McSweeney CS. 2006. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiol Ecol. 58:572–582.
   PubMed Web of Science ®Google Scholar
• Diaz HL, Karnati SKR, Lyons MA, Dehority BA, Firkins JL. 2014a. Chemotaxis toward carbohydrates and peptides by mixed ruminal protozoa when fed, fasted, or incubated with polyunsaturated fatty acids. J Dairy Sci. 97:2231–2243.
   PubMed Web of Science ®Google Scholar
• Diaz HL, Knapp JR, Karnati SKR, Dehority BA, Firkins JL. 2014b. Effects of wortmannin, sodium nitroprusside, insulin, genistein, and guanosine triphosphate on chemotaxis and cell growth of Entodinium caudatum, Epidinium caudatum, and mixed ruminal protozoa. J Dairy Sci. 97:2244–2253.
   PubMed Web of Science ®Google Scholar
• Dogiel VA. 1927. Monographie der Famille Ophryoscolecidae. Arch Portistkde. 59:1–282.
   Google Scholar
• Edwards JE, Kingston-Smith AH, Jimenez HR, Huws SA, Skot KP, Griffith GW, McEwan NR, Theodorou MK. 2008. Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen. FEMS Microbiol Ecol. 66:537–545.
   PubMed Web of Science ®Google Scholar
• Ellis JE, Williams AG, Lloyd D. 1989. Oxygen consumption by ruminal microorganisms: protozoal and bacterial contributions. Appl Environ Microbiol. 55:2583–2587.
   PubMed Web of Science ®Google Scholar
• Eugène M, Archimède H, Sauvant D. 2004. Quantitative meta-analysis on the effects of defaunation of the rumen on growth, intake and digestion in ruminants. Livest Prod Sci. 85:81–97.
   Google Scholar
• Ho YW, Abdullah N, Jalaludin S. 2000. The diversity and taxonomy of anaerobic gut fungi. Fungal Divers. 4:37–51.
   Google Scholar
• Hsu JT, Fahey GC, Merchen NR, Mackie RI. 1991. Effects of defaunation and various nitrogen supplementation regimens on microbial numbers and activity in the rumen of sheep. J Anim Sci. 69:1279–1289.
   PubMed Web of Science ®Google Scholar
• Huhtanen PP, Khalili H. 1992. The effect of sucrose supplements on particle-associated carboxymethylcellulase (EC 3.2.1.4) and xylanase (EC 3.2.1.8) activities in cattle given grass-silage-based diet. Brit J Nutr. 67:245–255.
   PubMed Web of Science ®Google Scholar
• Hungate RE. 1942. The culture of Eudiplodinium neglectum, with experiments on the digestion of cellulose. Biol Bull. 83:303–319.
   Google Scholar
• Jouany JP. 1989. Effects of diet on populations of rumen protozoa in relation to fiber digestion. In: Nolan JV, Leng RA, Demeyer DI, editors. The roles of protozoa and fungi in ruminant digestion. Proceedings of an International Seminar held at the University of New England; 1988 Sep 26–29; Armidale. Armidale: Penambul Books Armidale; p. 59–74.
   Google Scholar
• Jouany JP, Ushida K. 1990. Protozoa and fibre digestion in the rumen. In: Hoshino S, Onodera R, Mianto H, Itabashi H, editors. The rumen ecosystem: the microbial metabolism and its regulation. Tokyo: Japan Scientific Society Press; p. 139–151.
   Google Scholar
• Michałowski T. 1979. A simple system for continuous culture of rumen ciliates. Bull Pol Acad Sci Biol. 27:581–583.
   PubMed Web of Science ®Google Scholar
• Michałowski T. 1990. The synthesis and turnover of cellular matter of ciliates in the rumen. Acta Protozool. 29:47–72.
   Web of Science ®Google Scholar
• Michałowski T, Bełżecki G, Kwiatkowska E, Pająk JJ. 2003. The effect of selected rumen fauna on fibrolytic enzyme activities, bacterial mass, fibre disappearance and fermentation pattern in sheep. J Anim Feed Sci. 12:45–64.
   Web of Science ®Google Scholar
• Michałowski T, Harmeyer J, Bełżecki G. 1999. The importance of washing the omasum for successful defaunation of sheep. J Anim Feed Sci. 8:611–619.
   Web of Science ®Google Scholar
• Michałowski T, Kwiatkowska E, Pająk JJ. 1997. The effect of diet on the microbial mass, and xylanase and CMC-ase activites in whole rumen digesta and in different fractions of rumen contents of cows. J Anim Feed Sci. 6:485–497.
   Google Scholar
• Michalowski T, Muszynski P, Landa I. 1991. Factors influencing the growth of rumen ciliates Eudiplodinium maggii in vitro. Acta Protozool. 30:115–120.
   Web of Science ®Google Scholar
• Miller GL, Blum R, Glennon E, Burton A. 1960. Measurement of carboxymethylcellulase activity. Anal Biochem. 1:127–132.
   Web of Science ®Google Scholar
• Miltko R, Bełżecki G, Kowalik B, Michałowski T. 2014. Can fungal zoospores be the source of energy for the rumen protozoa Eudiplodinium maggii? Anaerobe. 29:68–72.
   PubMed Web of Science ®Google Scholar
• Miltko R, Belzecki G, Kowalik B, Skomial J. 2016. Presence of carbohydrate digesting enzymes throughout the digestive tract of sheep. Turk J Vet Anim Sci. 40:271–277.
   Web of Science ®Google Scholar
• Miltko R, Bełżecki G, Kwiatkowska E, Michałowski T. 2010. The ability of the rumen protozoan Eudiplodinium maggii to utilize chitin. Folia Microbiol. 55:349–351.
   PubMed Web of Science ®Google Scholar
• Miltko R, Kowalik B, Michałowski T, Bełżecki G. 2015a. Chitin as a source of energy for the rumen ciliates. J Anim Feed Sci. 24:203–207.
   Web of Science ®Google Scholar
• Miltko R, Pietrzak M, Bełżecki G, Wereszka K, Michałowski T, Hackstein JHP. 2015b. Isolation and in vitro cultivation of the fibrolytic rumen ciliate Eremoplastron (Eudiplodinium) dilobum. Eur J Protistol. 51:109–117.
   PubMed Web of Science ®Google Scholar
• Newbold CJ, Hillman K. 1990. The effect of ciliate protozoa on the turnover of bacterial and fungal protein in the rumen of sheep. Lett Appl Microbiol. 11:100–102.
   Web of Science ®Google Scholar
• Obispo NE, Dehority BA. 1992. A most probable number method for enumeration of rumen fungi with studies on factors affecting their concentration in the rumen. J Microbiol Methods. 16:259–270.
   Web of Science ®Google Scholar
• Orpin CG. 1974. The rumen flagellate Callimastix frontalis: does sequestration occur? J Gen Microbiol. 84:395–398.
   PubMedGoogle Scholar
• Orpin CG. 1975. Studies on the rumen flagellate Neocalimastix frontalis. J Gen Microbiol. 84:395–398.
   Google Scholar
• Orpin CG. 1977. Studies on the defaunation of the ovine rumen using dioctyl sodium sulphosuccinate. J Appl Bacteriol. 43:309–318.
   Google Scholar
• Orpin CG. 1984. The role of ciliate protozoa and fungi in the rumen digestion of plant cell walls. Anim Feed Sci Technol. 10:121–143.
   Web of Science ®Google Scholar
• Romulo BH, Bird SH, Leng RA. 1986. The effects of defaunation on digestibility and rumen fungi counts in sheep fed high fibre diets. Proc Aust SOC Anim Prod. 16:327–330.
   Google Scholar
• Shen CR, Chen YS, Yang CJ, Chen JK, Liu. 2010. Colloid chitin azure is a dispersible, low-cost substrate for chitinase measurements in a sensitive, fast, reproducible assay. J Biomol Screen. 15:213–217.
   PubMed Web of Science ®Google Scholar
• Simunek J, Hodrova B, Bartoova H, Kopecny J. 2001. Chitinolytic bacteria of the mammal digestive tract. Folia Microbiol. 46:76–78.
   PubMed Web of Science ®Google Scholar
• Soetanto H, Gordon GLR, Hume ID, Leng RA. 1985. The role of protozoa and fungi in fibre digestion in the rumen of sheep. In: The Scientific Committee of Congress, editor. Efficient animal production for Asian welfare. Proceedings of the Third Congress of the Asian-Australian Association of Animal Production Societies; 1985 May 6–10; Seoul, Korea: Australian Association of Animal Production Societies; p. 805–807.
   Google Scholar
• Theodorou MK, Lowman RS, Davies ZS, Cuddeford D, Owen E. 1998. Principles of technique that rely on gas maseurment in ruminant nutrition. In: Deaville ER, Owen E, Adesogan AT, Rymer C, Huntington JA, Lawrance TLJ, editors. In Vitro Techniques for Measuring Nutrient Supply to Ruminants. Proceedings of the International Symposium of the British Society of Animal Science; 1997 Jul 8–10; Reading (UK): British Society of Animal Science; Occasional Publication no. 22, p. 55–63.
   Google Scholar
• Ushida K, Jouany JP, Demeyer DI. 1991. Effects of presence or absence of rumen protozoa on the efficiency of utilisation of concentrate and fibrous feeds. In: Tsuda T, Sasaki Y, Kawashima R, editors. Physiological Aspects of Digestion and Metabolism in Ruminants. Proceedings of the Seventh International Symposium of Ruminant Physiology; 1989 Aug 28–Sep 1. San Diego (CA): Academic Press; p. 625–654.
   Google Scholar
• Ushida K, Kayouli C, De Smet S, Jouany JP. 1990. Effect of defaunation on protein and fibre digestion in sheep fed on ammonia-treated straw-based diets with or without maize. Brit J Nutr. 64:765–775.
   PubMed Web of Science ®Google Scholar
• Warner ACI. 1966. Diurnal changes in the concentrations of micro-organisms in the rumens of sheep fed limited diets once daily. J Gen Microbiol. 45:213–235.
   PubMedGoogle Scholar
• Williams AG, Withers SE. 1993. Changes in the rumen microbial population and its activities during the refaunation period after the reintroduction of ciliate protozoa into the rumen of defaunated sheep. Can J Microbiol. 39:61–69.
   PubMed Web of Science ®Google Scholar
• Wolin MJ, Miller TL, Stewart CS. 1997. Microbe-microbe interactions. In: Hobson PN, Stewart CS, editors. The rumen microbial ecosystem. 2nd ed. London: Blackie Academic & Professional; p. 467–491.
   Google Scholar