Elevated shear rate-induced by exercise increases eNOS ser¹¹⁷⁷ but not PECAM-1 Tyr⁷¹³ phosphorylation in human conduit artery endothelial cells

References

• Bagi, Z., Frangos, J. A., Yeh, J.-C., Charles R, W., Kaley, G., & Koller, A. (2005). PECAM-1 mediates NO-dependent dilation of arterioles to high temporal gradients of shear stress. Arteriosclerosis, Thrombosis, and Vascular Biology, 25(8), 1590–1595.
   PubMed Web of Science ®Google Scholar
• Balligand, J. L., Feron, O., & Dessy, C. (2009). eNOS activation by physical forces: From short-term regulation of contraction to chronic remodeling of cardiovascular tissues. Physiological Reviews, 89(2), 481–534.
   PubMed Web of Science ®Google Scholar
• Birukov, K. G. (2009). Cyclic stretch, reactive oxygen species, and vascular remodeling. Antioxidants & Redox Signaling, 11(7), 1651–1667.
   PubMed Web of Science ®Google Scholar
• Casey, D. P., Ueda, K., Wegman-Points, L., & Pierce, G. L. (2017). Muscle contraction induced arterial shear stress increases endothelial nitric oxide synthase phosphorylation in humans. American Journal of Physiology-Heart and Circulatory Physiology, 313(4), H854–h859.
   PubMed Web of Science ®Google Scholar
• Chatzizisis, Y. S., Coskun, A. U., Jonas, M., Edelman, E. R., Feldman, C. L., & Stone, P. H. (2007). Role of endothelial shear stress in the natural history of coronary atherosclerosis and vascular remodeling: Molecular, cellular, and vascular behavior. Journal of the American College of Cardiology, 49(25), 2379–2393.
   PubMed Web of Science ®Google Scholar
• Colombo, P. C., Ashton, A. W., Celaj, S., Talreja, A., Banchs, J. E., Dubois, N. B., … Le Jemtel, T. H. (2002). Biopsy coupled to quantitative immunofluorescence: A new method to study the human vascular endothelium. Journal of Applied Physiology (1985), 92(3), 1331–1338. doi:10.1152/japplphysiol.00680.2001
   PubMed Web of Science ®Google Scholar
• Dammers, R., Stifft, F., Tordoir, J. H. M., Hameleers, J. M., Hoeks, A. P. G., & Kitslaar, J. E. H. M. (2003). Shear stress depends on vascular territory: Comparison between common carotid and brachial artery. Journal of Applied Physiology (1985), 94(2), 485–489. doi:10.1152/japplphysiol.00823.2002
   PubMed Web of Science ®Google Scholar
• Davies, P. F. (2009). Hemodynamic shear stress and the endothelium in cardiovascular pathophysiology. Nature Clinical Practice Cardiovascular Medicine, 6(1), 16–26.
   PubMedGoogle Scholar
• Davignon, J., & Ganz, P. (2004). Role of endothelial dysfunction in atherosclerosis. Circulation, 109(23 Suppl 1), Iii27–Iii32. doi:10.1161/01.CIR.0000131515.03336.f8
   PubMed Web of Science ®Google Scholar
• Donato, A. J., Eskurza, I., Silver, A. E., Levy, A. S., Pierce, G. L., Gates, P. E., & Seals, D. R. (2007). Direct evidence of endothelial oxidative stress with aging in humans: Relation to impaired endothelium-dependent dilation and upregulation of nuclear factor-kappaB. Circulation Research, 100(11), 1659–1666.
   PubMed Web of Science ®Google Scholar
• Dusserre, N., L’Heureux, N., Bell, K. S., Stevens, H. Y., Yeh, J., Otte, L. A., … Frangos, J. A. (2004). PECAM-1 interacts with nitric oxide synthase in human endothelial cells: Implication for flow-induced nitric oxide synthase activation. Arteriosclerosis, Thrombosis, and Vascular Biology, 24(10), 1796–1802.
   PubMed Web of Science ®Google Scholar
• Feng, L., Stern, D. M., & Pile-Spellman, J. (1999). Human endothelium: Endovascular biopsy and molecular analysis. Radiology, 212(3), 655–664.
   PubMed Web of Science ®Google Scholar
• Fiorenza, M., Gliemann, L., Brandt, N., & Bangsbo, J. (2020). Hormetic modulation of angiogenic factors by exercise-induced mechanical and metabolic stress in human skeletal muscle. American Journal of Physiology-Heart and Circulatory Physiology, 319(4), H824–h834.
   PubMed Web of Science ®Google Scholar
• Fleming, I., Fisslthaler, B., Dixit, M., & Busse, R. (2005). Role of PECAM-1 in the shear-stress-induced activation of Akt and the endothelial nitric oxide synthase (eNOS) in endothelial cells. Journal of Cell Science, 118(Pt 18), 4103–4111.
   PubMed Web of Science ®Google Scholar
• Gliemann, L., Rytter, N., Lindskrog, M., Slingsby, M. H. L., Åkerström, T., Sylow, L., … Hellsten, Y. (2017). Endothelial mechanotransduction proteins and vascular function are altered by dietary sucrose supplementation in healthy young male subjects. The Journal of Physiology, 595(16), 5557–5571.
   PubMed Web of Science ®Google Scholar
• Gliemann, L., Rytter, N., Piil, P., Nilton, J., Lind, T., Nyberg, M., … Hellsten, Y. (2018). The endothelial mechanotransduction protein platelet endothelial cell adhesion molecule-1 is influenced by aging and exercise training in human skeletal muscle. Frontiers in Physiology, 9, 1807.
   PubMed Web of Science ®Google Scholar
• Green, D. J., Hopkins, N. D., Jones, H., Thijssen, D. H. J., Eijsvogels, T. M. H., & Yeap, B. B. (2016). Sex differences in vascular endothelial function and health in humans: Impacts of exercise. Experimental Physiology, 101(2), 230–242.
   PubMed Web of Science ®Google Scholar
• Green, D. J., Hopman, M. T. E., Padilla, J., Laughlin, M. H., & Thijssen, D. H. J. (2017). Vascular adaptation to exercise in humans: Role of hemodynamic stimuli. Physiological Reviews, 97(2), 495–528.
   PubMed Web of Science ®Google Scholar
• Green, D J, Maiorana, A, O'driscoll, G, & Taylor, R. (2004). Effect of exercise training on endothelium-derived nitric oxide function in humans. The Journal of Physiology, 561(1), 1–25.
   PubMed Web of Science ®Google Scholar
• Gündisch, S., Annaratone, L., Beese, C., Drecol, E., Marchiò, C., Quaglino, E., … Bussolati, G. (2015). Critical roles of specimen type and temperature before and during fixation in the detection of phosphoproteins in breast cancer tissues. Laboratory Investigation, 95(5), 561–571.
   PubMed Web of Science ®Google Scholar
• Hambrecht, R., Adams, V., Erbs, S., Linke, A., Kränkel, N., Shu, Y., … Schuler, G. (2003). Regular physical activity improves endothelial function in patients with coronary artery disease by increasing phosphorylation of endothelial nitric oxide synthase. Circulation, 107(25), 3152–3158.
   PubMed Web of Science ®Google Scholar
• Hellsten, Y., & Gliemann, L. (2018). Limb vascular function in women – effects of female sex hormones and physical activity. Translational Sports Medicine, 1(1), 14–24.
   Google Scholar
• Newman, P. J. (1999). Switched at birth: A new family for PECAM-1. Journal of Clinical Investigation, 103(1), 5–9.
   PubMed Web of Science ®Google Scholar
• Osawa, M., Masuda, M., Harada, N., Lopes, R. B., & Fujiwara, K. (1997). Tyrosine phosphorylation of platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) in mechanically stimulated vascular endothelial cells. European Journal of Cell Biology, 72(3), 229–237.
   PubMed Web of Science ®Google Scholar
• Park, S.-K., La Salle, D. T., Cerbie, J., Cho, J. M., Bledsoe, A., Nelson, A., … Symons, J. D. (2019). Elevated arterial shear rate increases indexes of endothelial cell autophagy and nitric oxide synthase activation in humans. American Journal of Physiology-Heart and Circulatory Physiology, 316(1), H106–H112.
   PubMed Web of Science ®Google Scholar
• Pierce, G. L., Donato, A. J., LaRocca, T. J., Eskurza, I., Silver, A. E., & Seals, D. R. (2011). Habitually exercising older men do not demonstrate age-associated vascular endothelial oxidative stress. Aging Cell, 10(6), 1032–1037.
   PubMed Web of Science ®Google Scholar
• Sandoo, A., Veldhuijzen van Zanten, J. J. C. S., Metsios, G. S., Carroll, D., & Kitas, G. D. (2010). The endothelium and its role in regulating vascular tone. The Open Cardiovascular Medicine Journal, 4, 302–312.
   PubMedGoogle Scholar
• Thijssen, D. H. J., Bruno, R. M., van Mil, A. C. C. M., Holder, S. M., Faita, F., Greyling, A., … Ghiadoni, L. (2019). Expert consensus and evidence-based recommendations for the assessment of flow-mediated dilation in humans. European Heart Journal, 40(30), 2534–2547.
   PubMed Web of Science ®Google Scholar
• Thijssen, D. H. J., Black, M. A., Pyke, K. E., Padilla, J., Atkinson, G., Harris, R. A., … Green, D. J. (2011). Assessment of flow-mediated dilation in humans: A methodological and physiological guideline. American Journal of Physiology-Heart and Circulatory Physiology, 300(1), H2–H12.
   PubMed Web of Science ®Google Scholar
• Thijssen, D. H. J., Dawson, E. A., Black, M. A., Hopman, M. T. E., Cable, N. T., & Green, D. J. (2009). Brachial artery blood flow responses to different modalities of lower limb exercise. Medicine & Science in Sports & Exercise, 41(5), 1072–1079.
   PubMed Web of Science ®Google Scholar
• Tremblay, J. C., Ainslie, P. N., Turner, R., Gatterer, H., Schlittler, M., Woyke, S., … Siebenmann, C. (2020). Endothelial function and shear stress in hypobaric hypoxia: Time course and impact of plasma volume expansion in men. American Journal of Physiology-Heart and Circulatory Physiology, 319(5), H980–H994.
   PubMed Web of Science ®Google Scholar
• Tryfonos, A., Cocks, M., Mills, J., Green, D. J., & Dawson, E. A. (2020a). Exercise-induced vasodilation is not impaired following radial artery catheterization in coronary artery disease patients. Journal of Applied Physiology (1985), 128(2), 422–428. doi:10.1152/japplphysiol.00695.2019
   PubMed Web of Science ®Google Scholar
• Tryfonos, A., Cocks, M., Rasoul, D., Mills, J., Green, D. J., & Dawson, E. A. (2020b). Impact of catheterization on shear-mediated arterial dilation in healthy young men. European Journal of Applied Physiology, 120(11), 2525–2532.
   PubMed Web of Science ®Google Scholar
• Tzima, E., Irani-Tehrani, M., Kiosses, W. B., Dejana, E., Schultz, D. A., Engelhardt, B., … Schwartz, M. A. (2005). A mechanosensory complex that mediates the endothelial cell response to fluid shear stress. Nature, 437(7057), 426–431.
   PubMed Web of Science ®Google Scholar
• Wang, S., Chennupati, R., Kaur, H., Iring, A., Wettschureck, N., & Offermanns, S. (2016). Endothelial cation channel PIEZO1 controls blood pressure by mediating flow-induced ATP release. Journal of Clinical Investigation, 126(12), 4527–4536.
   PubMed Web of Science ®Google Scholar
• Wang, Y., Zhang, Y., Hu, W., Xie, S., Gong, C.-X., Iqbal, K., & Liu, F. (2015). Rapid alteration of protein phosphorylation during postmortem: Implication in the study of protein phosphorylation. Scientific Reports, 5(1), 1–12.
   Google Scholar
• Wilson, C., Lee, M. D., & McCarron, J. G. (2016). Acetylcholine released by endothelial cells facilitates flow-mediated dilatation. The Journal of Physiology, 594(24), 7267–7307.
   PubMed Web of Science ®Google Scholar
• Woodman, R. J., Playford, D. A., Watts, G. F., Cheetham, C., Reed, C., Taylor, R. R., … Green, D. (2001). Improved analysis of brachial artery ultrasound using a novel edge-detection software system. Journal of Applied Physiology (1985), 91(2), 929–937. doi:10.1152/jappl.2001.91.2.929
   PubMed Web of Science ®Google Scholar
• Xu, J., Mathur, J., Vessières, E., Hammack, S., Nonomura, K., Favre, J., … Patapoutian, A. (2018). GPR68 senses flow and Is essential for vascular physiology. Cell, 173(3), 762–775.e16. e716.
   PubMed Web of Science ®Google Scholar