Efficient expression of a cnidarian peptide-gated ion channel in mammalian cells

References

• Golubovic A, Kuhn A, Williamson M, et al. A peptide-gated ion channel from the freshwater polyp Hydra. J Biol Chem. 2007 Nov 30;282(48):35098–35103.
   PubMed Web of Science ®Google Scholar
• Assmann M, Kuhn A, Dürrnagel S, et al. The comprehensive analysis of DEG/ENaC subunits in Hydra reveals a large variety of peptide-gated channels, potentially involved in neuromuscular transmission. BMC Biol. 2014;12:84.
   PubMed Web of Science ®Google Scholar
• Dürrnagel S, Kuhn A, Tsiairis CD, et al. Three homologous subunits form a high affinity peptide-gated ion channel in Hydra. J Biol Chem. 2010 Apr 16;285(16):11958–11965.
   PubMed Web of Science ®Google Scholar
• Moosler A, Rinehart KL, Grimmelikhuijzen CJ. Isolation of four novel neuropeptides, the hydra-RFamides I-IV, from Hydra magnipapillata. Biochem Biophys Res Commun. 1996;229(2):596–602.
   PubMed Web of Science ®Google Scholar
• Gründer S, Assmann M. Peptide-gated ion channels and the simple nervous system of Hydra. J Exp Biol. 2015 Feb 15;218(Pt 4):551–561.
   PubMed Web of Science ®Google Scholar
• Dürrnagel S, Falkenburger BH, Gründer S. High Ca(2+) permeability of a peptide-gated DEG/ENaC from Hydra. J Gen Physiol. 2012 Oct;140(4):391–402.
   PubMed Web of Science ®Google Scholar
• Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996 Mar 1;6(3):315–324.
   PubMed Web of Science ®Google Scholar
• Sharp PM, Li WH. The codon adaptation index - a measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acids Res. 1987 Feb 11;15(3):1281–1295.
   PubMed Web of Science ®Google Scholar
• Slimko EM, Lester HA. Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels. J Neurosci Meth. 2003 Mar 30;124(1):75–81.
   PubMed Web of Science ®Google Scholar
• Waldmann R, Bassilana F, de Weille J, et al. Molecular cloning of a non-inactivating proton-gated Na+ channel specific for sensory neurons. J Biol Chem. 1997;272(34):20975–20978.
   PubMed Web of Science ®Google Scholar
• Chen CC, England S, Akopian AN, et al. A sensory neuron-specific, proton-gated ion channel. Proc Natl Acad Sci U S A. 1998;95(17):10240–10245.
   PubMed Web of Science ®Google Scholar
• Sutherland SP, Benson CJ, Adelman JP, et al. Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons. Proc Natl Acad Sci U S A. 2001;98(2):711–716.
   PubMed Web of Science ®Google Scholar
• Badhey MLO, Bertalovitz AC, McDonald TV. Express with caution: epitope tags and cDNA variants effects on hERG channel trafficking, half-life and function. J Cardiovasc Electrophysiol. 2017 Sep;28(9):1070–1082.
   PubMed Web of Science ®Google Scholar
• Ikemura T. Codon usage and transfer-rna content in unicellular and multicellular organisms. Mol Biol Evol. 1985;2(1):13–34.
   PubMed Web of Science ®Google Scholar
• Kanaya S, Yamada Y, Kinouchi M, et al. Codon usage and tRNA genes in eukaryotes: correlation of codon usage diversity with translation efficiency and with CG-dinucleotide usage as assessed by multivariate analysis. J Mol Evol. 2001 Oct-Nov;53(4–5):290–298.
   PubMed Web of Science ®Google Scholar
• Kane JF. Effects of Rare Codon Clusters on High-Level Expression of Heterologous Proteins in Escherichia-Coli. Curr Opin Biotech. 1995 Oct;6(5):494–500.
   PubMed Web of Science ®Google Scholar
• Nakamura Y, Gojobori T, Ikemura T. Codon usage tabulated from the international DNA sequence databases. Nucleic Acids Res. 1998 Jan 1;26(1):334.
   PubMed Web of Science ®Google Scholar
• Oconnell P, Rosbash M. Sequence, structure, and codon preference of the drosophila ribosomal protein-49 gene. Nucleic Acids Res. 1984;12(13):5495–5513.
   PubMed Web of Science ®Google Scholar
• Cannarrozzi G, Schraudolph NN, Faty M, et al. A role for codon order in translation dynamics. Cell. 2010 Apr 16;141(2):355–367.
   PubMed Web of Science ®Google Scholar
• Ou KC, Wang CY, Liu KT, et al. Optimization protein productivity of human interleukin-2 through codon usage, gene copy number and intracellular tRNA concentration in CHO cells. Biochem Biophys Res Commun. 2014 Nov 14;454(2):347–352.
   PubMed Web of Science ®Google Scholar
• Zhong F, Cao WP, Chan E, et al. Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression. Immunology. 2005 Jan;114(1):83–93.
   PubMed Web of Science ®Google Scholar
• Gvritishvili AG, Leung KW, Tombran-Tink J. Codon preference optimization increases heterologous PEDF expression. Plos One. 2010 Nov 30;5(11):e15056.
   PubMed Web of Science ®Google Scholar
• Wang Q, Mei C, Zhen HH, et al. Codon preference optimization increases prokaryotic cystatin C expression. J Biomed Biotechnol. 2012;2012:732017.
   Google Scholar
• Wells KD, Foster JA, Moore K, et al. Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch. Transgenic Res. 1999 Oct;8(5):371–381.
   PubMed Web of Science ®Google Scholar
• Qian BJ, Shen HF, Xiong JL, et al. Expression and purification of the synthetic preS1 gene of Hepatitis B virus with preferred Escherichia coli codon preference. Protein Expression Purif. 2006 Jul;48(1):74–80.
   PubMed Web of Science ®Google Scholar
• Frelin L, Ahlen G, Alheim M, et al. Codon optimization and mRNA amplification effectively enhances the immunogenicity of the hepatitis C virus nonstructural 3/4A gene. Gene Ther. 2004 Mar;11(6):522–533.
   PubMed Web of Science ®Google Scholar
• Kühn FJP, Kühn C, Lückhoff A. Functional characterisation of a TRPM2 orthologue from the sea anemone Nematostella vectensis in human cells. Sci Rep-Uk. 2015 Jan 26;5:8032.
   Web of Science ®Google Scholar
• Clarke TF, Clark PL. Rare Codons Cluster. Plos One. 2008 Oct 15;3(10):e3412.
   Web of Science ®Google Scholar
• Rodriguez A, Wright G, Emrich S, et al. %MinMax: a versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding. Protein Sci. 2017;27(1):356–362.
   PubMed Web of Science ®Google Scholar
• Cully DF, Vassilatis DK, Liu KK, et al. Cloning of an avermectin-sensitive glutamate-gated chloride channel from caenorhabditis-elegans. Nature. 1994 Oct 20;371(6499):707–711.
   PubMed Web of Science ®Google Scholar
• Martinson AS, van Rossum DB, Diatta FH, et al. Functional evolution of Erg potassium channel gating reveals an ancient origin for I-Kr. Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5712–5717.
   PubMed Web of Science ®Google Scholar
• Li X, Liu H, Luo JC, et al. Major diversification of voltage-gated K+ channels occurred in ancestral parahoxozoans. Proc Natl Acad Sci U S A. 2015 Mar 3;112(9):E1010–E1019.
   PubMed Web of Science ®Google Scholar
• Baker EC, Layden MJ, van Rossum DB, et al. Functional characterization of Cnidarian HCN channels points to an early evolution of I-h. Plos One. 2015 Nov 10;10(11):e0142730.
   Web of Science ®Google Scholar
• Jegla T, Grigoriev N, Gallin WJ, et al. Multiple shaker potassium channels in a primitive metazoan. J Neurosci. 1995 Dec;15(12):7989–7999.
   PubMed Web of Science ®Google Scholar
• Barzilai MG, Reitzel AM, Kraus JEM, et al. Convergent evolution of sodium ion selectivity in metazoan neuronal signaling. Cell Rep. 2012 Aug;2(2):242–248.
   PubMed Web of Science ®Google Scholar
• Elkhatib W, Smith CL, Senatore A. A Na+ leak channel cloned from Trichoplax adhaerens extends extracellular pH and Ca2+ sensing for the DEG/ENaC family close to the base of Metazoa. J Biol Chem. 2019 Nov 1;294(44):16320–16336.
   PubMed Web of Science ®Google Scholar
• Bosch TCG, Klimovich A, Domazet-Loso T, et al. Back to the Basics: cnidarians Start to Fire. Trends Neurosci. 2017 Feb;40(2):92–105.
   PubMed Web of Science ®Google Scholar
• DeLaney K, Buchberger AR, Atkinson L, et al. New techniques, applications and perspectives in neuropeptide research. J Exp Biol. 2018 Feb;221(3):jeb151167.
   PubMed Web of Science ®Google Scholar