The anchor domain is critical for Piezo1 channel mechanosensitivity

References

• Martinac B, Cox CD. Comprehensive Biophysics. Vol. 7. London: Elsevier; 2017.
   Google Scholar
• Cox CD, Bavi N, Martinac B. Bacterial mechanosensors. Annu Rev Physiol. 2018;80. DOI:https://doi.org/10.1146/annurev-physiol-021317-121351.
   Google Scholar
• Kloda A, Martinac B. Mechanosensitive channels of bacteria and archaea share a common ancestral origin. Eur Biophys J. 2002;31:14–25.
   PubMed Web of Science ®Google Scholar
• Douguet D, Honore E. Mammalian mechanoelectrical transduction: structure and function of force-gated ion channels. Cell. 2019;179:340–354.
   PubMed Web of Science ®Google Scholar
• Martinac B, Buechner M, Delcour AH, et al. Pressure-sensitive ion channel in Escherichia coli. Proc Natl Acad Sci U S A. 1987;84:2297–2301.
   PubMed Web of Science ®Google Scholar
• Sukharev SI, Blount P, Martinac B, et al. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994;368:265–268.
   PubMed Web of Science ®Google Scholar
• Martinac B, Adler J, Kung C. Mechanosensitive ion channels of E. coli activated by amphipaths. Nature. 1990;348:261–263.
   PubMed Web of Science ®Google Scholar
• Kung C. A possible unifying principle for mechanosensation. Nature. 2005;436:647–654.
   PubMed Web of Science ®Google Scholar
• Cox CD, Bae C, Ziegler L, et al. Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension. Nat Commun. 2016;7(10366). DOI:https://doi.org/10.1038/ncomms10366
   Google Scholar
• Brohawn SG, Su Z, MacKinnon R. Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K+ channels. Proc Natl Acad Sci U S A. 2014;111:3614–3619.
   PubMed Web of Science ®Google Scholar
• Dong YY, Pike ACW, Mackenzie A, et al. K2P channel gating mechanisms revealed by structures of TREK-2 and a complex with Prozac. Science. 2015;347(6227):1256–1259.
   Web of Science ®Google Scholar
• Murthy SE, Dubin AE, Whitwam T, et al. OSCA/TMEM63 are an evolutionarily conserved family of mechanically activated ion channels. eLife. 2018;7. DOI:https://doi.org/10.7554/eLife.41844.
   Web of Science ®Google Scholar
• Syeda R, Florendo MN, Cox, CD, et al. Piezo1 channels are inherently mechanosensitive. Cell Rep. 2016;17(7). DOI:https://doi.org/10.1016/j.celrep.2016.10.033
   Google Scholar
• Bavi N, Cox CD, Perozo E, et al. Toward a structural blueprint for bilayer-mediated channel mechanosensitivity. Channels (Austin). 2018;7(91):91–93.
   Google Scholar
• Bavi N, Cortes DM, Cox CD, et al. The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels. Nat Commun. 2016;7(1). DOI:https://doi.org/10.1038/ncomms11984
   Google Scholar
• Iscla I, Wray R, Eaton C, et al. Scanning MscL channels with targeted post-translational modifications for functional alterations. PloS One. 2017;11(2):e0137994.
   Google Scholar
• Iscla I, Wray R, Blount P. On the structure of the N-terminal domain of the MscL channel: helical bundle or membrane interface. Biophys J. 2008;95:2283–2291.
   PubMed Web of Science ®Google Scholar
• Honoré E, Maingret F, Lazdunski M, et al. An intracellular proton sensor commands lipid- and mechano-gating of the K(+) channel TREK-1. EMBO J. 2002;21:2968–2976.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Daday C, Gu R-X, et al. Visualizing the mechanosensitive ion channel MscS under membrane tension. Nature. 2021;590:509–514.
   Web of Science ®Google Scholar
• Saotome K, Murthy SE, Kefauver JM, et al. Structure of the mechanically activated ion channel Piezo1. Nature. 2018;554(481–486). DOI:https://doi.org/10.1038/nature25453
   Google Scholar
• Erdogmus S, Storch U, Danner L, et al. Helix 8 is the essential structural motif of mechanosensitive GPCRs. Nat Commun. 2019;10(5784). DOI:https://doi.org/10.1038/s41467-019-13722-0
   Google Scholar
• Steinbacher S, Bass R, Strop P, et al. In: Hamill OP, ed. Mechanosensitive Ion Channels, Part A. Elsevier Academic Press, Inc. Cambridge, Massachusetts; 2007. p. 1–24.
   Google Scholar
• Guo YR, MacKinnon R. Structure-based membrane dome mechanism for Piezo mechanosensitivity. eLife. 2017;6. DOI:https://doi.org/10.7554/eLife.33660.
   Google Scholar
• Zhao Q, Zhou H, Chi S, et al. Structure and mechanogating mechanism of the Piezo1 channel. Nature. 2018;554:487–492.
   PubMed Web of Science ®Google Scholar
• Li J, et al. Piezo1 integration of vascular architecture with physiological force. Nature. 2014;515:279–282.
   PubMed Web of Science ®Google Scholar
• Douguet D, Patel A, Xu A, et al. Piezo Ion Channels in Cardiovascular Mechanobiology. Trends Pharmacol Sci. 2019;40:956–970.
   PubMed Web of Science ®Google Scholar
• Lai A, Chen YC, Cox CD, et al. Analyzing the shear-induced sensitization of mechanosensitive ion channel Piezo-1 in human aortic endothelial cells. J Cell Physiol. 2021;236:2976–2987.
   PubMed Web of Science ®Google Scholar
• Prole DL, Taylor CW. Identification and analysis of putative homologues of mechanosensitive channels in pathogenic protozoa. PloS One. 2013;8(e66068). DOI:https://doi.org/10.1371/journal.pone.0066068
   Google Scholar
• Lukacs V, Mathur J, Mao R, et al. Impaired PIEZO1 function in patients with a novel autosomal recessive congenital lymphatic dysplasia. Nat Commun. 2015;6(8329). DOI:https://doi.org/10.1038/ncomms9329
   Google Scholar
• Bae C, Gnanasambandam R, Nicolai C, et al. Xerocytosis is caused by mutations that alter the kinetics of the mechanosensitive channel PIEZO1. Proc Natl Acad Sci U S A. 2013;110:E1162–1168.
   PubMed Web of Science ®Google Scholar
• Buyan A, Cox CD, Barnoud J, et al. Piezo1 forms specific, functionally important interactions with phosphoinositides and cholesterol. Biophys J. 2020;119:1683–1697.
   PubMed Web of Science ®Google Scholar
• Li JV, Ng C-A, Cheng D, et al. Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutations. bioRxiv. 2020;404962. 2020.2011.2030. doi:https://doi.org/10.1101/2020.11.30.404962
   Google Scholar
• Zamyatnin AA. Amino acid, peptide, and protein volume in solution. Annu Rev Biophys Bioeng. 1984;13:145–165.
   PubMedGoogle Scholar
• Janin J. Surface and inside volumes in globular proteins. Nature. 1979;277:491–492.
   PubMed Web of Science ®Google Scholar
• Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982;157:105–132.
   PubMed Web of Science ®Google Scholar
• Engelman DM, Steitz TA, Goldman A. Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annual rev biophysics biophysical chem. 1986;15(321–353). DOI:https://doi.org/10.1146/annurev.bb.15.060186.001541
   Google Scholar
• Jin P, Jan LY, Jan YN. Mechanosensitive ion channels: structural features relevant to mechanotransduction mechanisms. Annu Rev Neurosci. 2020;43:207–229.
   PubMed Web of Science ®Google Scholar
• Cox CD, Bavi N, Martinac B. Biophysical principles of ion-channel-mediated mechanosensory transduction. Cell Rep. 2019;29:1–12.
   PubMed Web of Science ®Google Scholar
• Sukharev S. Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes. Biophys J. 2002;83:290–298.
   PubMed Web of Science ®Google Scholar
• Cox CD, Bavi N, Martinac B. Current Topics in Membranes. Cambridge: Academic Press; 2016.
   Google Scholar
• Andolfo I, Alper SL, De Franceschi L, et al. Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1. Blood. 2013;121(3925–3935):S3921–3912.
   Google Scholar
• Nosyreva ED, Thompson D, Syeda R. Identification and functional characterization of the Piezo1 channel pore domain. J Biol Chem. 2021;296(100225). DOI:https://doi.org/10.1074/jbc.RA120.015905
   Google Scholar
• Zhao Q, Wu K, Geng J, et al. Ion permeation and mechanotransduction mechanisms of mechanosensitive Piezo Channels. Neuron. 2016;89:1248–1263.
   PubMed Web of Science ®Google Scholar
• Lin Y-C, Guo YR, Miyag A, et al. Force-induced conformational changes in PIEZO1. Nature. 2019;573:230–234.
   PubMed Web of Science ®Google Scholar
• Jiang Y, Yang X, Jiang J, et al. Structural designs and mechanogating mechanisms of the mechanosensitive Piezo channels. Trends Biochem Sci. 2021. DOI:https://doi.org/10.1016/j.tibs.2021.01.008
   Google Scholar
• Bavi N, Richardson J, Heu C, et al. PIEZO1-mediated currents are modulated by substrate mechanics. ACS Nano. 2019;13:13545–13559.
   PubMed Web of Science ®Google Scholar
• Poole K, Herget R, Lapatsina L, et al. Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch. Nat Commun. 2014;5(3520). DOI:https://doi.org/10.1038/ncomms4520
   Google Scholar