Blowing in the wind: Bacteria and fungi are spreading from public restroom hand dryers

References

• World Health Organization (WHO). Guidelines on hand hygiene in healthcare. Clean hands are safe hands. http://www.who.int/patientsafety/events/05/HH_en.pdf. Accessed May 09, 2020.
   Google Scholar
• United States Centers for Disease Control and Prevention. Guideline for hand hygiene in health-care settings. Recommendations of the Healthcare Infection Control Practices Advisory Committee and the HICPAC/SHEA/APIC/IDSA Hand Hygiene Task Force. MMWR Morb Mortal Wkly Rep. 2002;51 (RR-16):1–48.
   Google Scholar
• United States Centers for Disease Control and Prevention. Handwashing: Clean hands save lives. Celebrate Global Handwashing Day to promote handwashing with soap in your community and around the world. https://www.cdc.gov/handwashing/global-handwashing-day.html. Accessed May 15, 2020.
   Google Scholar
• Bolon MK. Hand hygiene: An update. Infect Dis Clin North Am. 2016;30(3):591–607. doi:10.1016/j.idc.2016.04.007.
   PubMed Web of Science ®Google Scholar
• Huang C, Ma W, Stack S. The hygienic efficacy of different hand-drying methods: a review of the evidence. Mayo Clin Proc. 2012;87(8):791–798. doi:10.1016/j.mayocp.2012.02.019.
   PubMed Web of Science ®Google Scholar
• Pittet D, Allegranzi B, Sax H, et al. Evidence-based model for hand transmission during patient care and the role of improved practices. Lancet Infect Dis. 2006;6(10):641–652. doi:10.1016/S1473-3099(06)70600-4.
   PubMed Web of Science ®Google Scholar
• Gould D. The significance of hand-drying in the prevention of infection. Nurs Times. 1994;90(47):33–35.
   PubMedGoogle Scholar
• Alharbi SA, Salmen SH, Chinnathambi A, et al. Assessment of the bacterial contamination of hand air dryer in washrooms. Saudi J Biol Sci. 2016;23(2):268–271. doi:10.1016/j.sjbs.2015.06.020.
   PubMed Web of Science ®Google Scholar
• Best EL, Parnell P, Wilcox MH. Microbiological comparison of hand-drying methods: the potential for contamination of the environment, user, and bystander. J Hosp Infect. 2014;88(4):199–206. doi:10.1016/j.jhin.2014.08.002.
   PubMed Web of Science ®Google Scholar
• Best EL, Redway K. Comparison of different hand-drying methods: the potential for airborne microbe dispersal and contamination. J Hosp Infect. 2015;89(3):215–217. doi:10.1016/j.jhin.2014.11.007.
   PubMed Web of Science ®Google Scholar
• Del Carmen Huesca-Espitia L, Aslanzadeh J, Feinn R, Joseph G, Murray TS, Setlow P. Deposition of bacteria and bacterial spores by bathroom hot air hand dryers. Appl Environ Microbiol. 2018;84(8):e00044–00018. doi:10.1128/AEM.00044-18.
   PubMed Web of Science ®Google Scholar
• Hanna JN, Loewenthal MR, Negel P, Wenck DJ. An outbreak of hepatitis A in an intensive care unit. Anaesth Intensive Care. 1996;24(4):440–444. doi:10.1177/0310057X9602400405.
   PubMed Web of Science ®Google Scholar
• Best EL, Parnell P, Couturier J, et al. Environmental contamination by bacteria in hospital washrooms according to hand-drying method: a multi-centre study. J Hosp Infect. 2018;100(4):469–475. doi:10.1016/j.jhin.2018.07.002.
   PubMed Web of Science ®Google Scholar
• Lax S, Gilbert JA. Hospital-associated microbiota and implications for nosocomial infections. Trends Mol Med. 2015;21(7):427–432. doi:10.1016/j.molmed.2015.03.005.
   PubMed Web of Science ®Google Scholar
• Lax S, Sangwan N, Smith D, et al. Bacterial colonization and succession in a newly opened hospital. Sci Transl Med. 2017;9(391):1–11. doi:10.1126/scitranslmed.aah6500.
   Web of Science ®Google Scholar
• Tong X, Xu H, Zou L, et al. High diversity of airborne fungi in the hospital environment as revealed by meta-sequencing-based microbiome analysis. Sci Rep. 2017;7:39606. doi:10.1038/srep39606.
   PubMed Web of Science ®Google Scholar
• Baker GC, Smith JJ, Cowan DA. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods. 2003;55(3):541–555. doi:10.1016/j.mimet.2003.08.009.
   PubMed Web of Science ®Google Scholar
• Daims H, Bruhl A, Amann R, Schleifer KH, Wagner M. The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol. 1999;22(3):434–444. doi:10.1016/S0723-2020(99)80053-8.
   PubMed Web of Science ®Google Scholar
• Thomas MC, Thomas DK, Selinger LB, Inglis GD. Spyder, a new method for in silico design and assessment of 16S rRNA gene primers for molecular microbial ecology. FEMS Microbiol Lett. 2011;320(2):152–159. doi:10.1111/j.1574-6968.2011.02302.x.
   PubMed Web of Science ®Google Scholar
• Turenne CY, Sanche SE, Hoban DJ, Karlowsky JA, Kabani AM. Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system. J Clin Microbiol. 1999;37(6):1846–1851. doi:10.1128/JCM.37.6.1846-1851.1999.
   PubMed Web of Science ®Google Scholar
• White TJ. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press, Inc.; 1990:315–322.
   Google Scholar
• Fu X, Li Y, Yuan Q, et al. Continental-scale microbiome study reveals different environmental characteristics determining microbial richness, composition, and quantity in hotel rooms. mSystems. 2020;5(3):1–15. doi:10.1128/mSystems.00119-20.
   Web of Science ®Google Scholar
• Lax S, Smith DP, Hampton-Marcell J, et al. Longitudinal analysis of microbial interaction between humans and the indoor environment. Science. 2014;345(6200):1048–1052. doi:10.1126/science.1254529.
   PubMed Web of Science ®Google Scholar
• Mbareche H, Veillette M, Bilodeau G, Duchaine C. Comparison of the performance of ITS1 and ITS2 as barcodes in amplicon-based sequencing of bioaerosols. PeerJ. 2020;8:e8523. doi:10.7717/peerj.8523.
   PubMed Web of Science ®Google Scholar
• Op De Beeck M, Lievens B, Busschaert P, Declerck S, Vangronsveld J, Colpaert JV. Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies. PLoS One. 2014;9(6):e97629. doi:10.1371/journal.pone.0097629.
   PubMed Web of Science ®Google Scholar
• Helgason E, Tourasse NJ, Meisal R, Caugant DA, Kolsto AB. Multilocus sequence typing scheme for bacteria of the Bacillus cereus group. Appl Environ Microbiol. 2004;70(1):191–201. doi:10.1128/aem.70.1.191-201.2004.
   PubMed Web of Science ®Google Scholar
• Sivadon V, Rottman M, Chaverot S, et al. Use of genotypic identification by sodA sequencing in a prospective study to examine the distribution of coagulase-negative Staphylococcus species among strains recovered during septic orthopedic surgery and evaluate their significance. J Clin Microbiol. 2005;43(6):2952–2954. doi:10.1128/JCM.43.6.2952-2954.2005.
   PubMed Web of Science ®Google Scholar
• Vilas-Boas G, Sanchis V, Lereclus D, Lemos MV, Bourguet D. Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis. Appl Environ Microbiol. 2002;68(3):1414–1424. doi:10.1128/aem.68.3.1414-1424.2002.
   PubMed Web of Science ®Google Scholar
• Stielow JB, Levesque CA, Seifert KA, et al. One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes. Persoonia. 2015;35:242–263. doi:10.3767/003158515X689135.
   PubMed Web of Science ®Google Scholar
• Wang L, Yokoyama K, Miyaji M, Nishimura K. The identification and phylogenetic relationship of pathogenic species of Aspergillus based on the mitochondrial cytochrome b gene. Med Mycol. 1998;36(3):153–164. doi:10.1080/02681219880000231.
   PubMed Web of Science ®Google Scholar
• Kulik T, Abarenkov K, Buśko M, et al. ToxGen: an improved reference database for the identification of type B-trichothecene genotypes in Fusarium. PeerJ. 2017;5:e2992. doi:10.7717/peerj.2992.
   PubMed Web of Science ®Google Scholar
• Snelling AM, Saville T, Stevens D, Beggs CB. Comparative evaluation of the hygienic efficacy of an ultra-rapid hand dryer vs conventional warm air hand dryers. J Appl Microbiol. 2011;110(1):19–26. doi:10.1111/j.1365-2672.2010.04838.x.
   PubMed Web of Science ®Google Scholar
• Bensch K, Groenewald JZ, Meijer M, et al. Cladosporium species in indoor environments. Stud Mycol. 2018;89:177–301. doi:10.1016/j.simyco.2018.03.002.
   PubMedGoogle Scholar
• Pearce TL, Scott JB, Crous PW, Pethybridge SJ, Hay FS. Tan spot of pyrethrum is caused by a Didymella species complex. Plant Pathol. 2016;65(7):1170–1184. doi:10.1111/ppa.12493.
   Web of Science ®Google Scholar
• Perrone G, Susca A. Penicillium species and their associated mycotoxins. Methods Mol Biol. 2017;1542:107–119. doi:10.1007/978-1-4939-6707-0_5.
   PubMedGoogle Scholar
• Prasongsuk S, Lotrakul P, Ali I, Bankeeree W, Punnapayak H. The current status of Aureobasidium pullulans in biotechnology. Folia Microbiol (Praha). 2018;63(2):129–140. doi:10.1007/s12223-017-0561-4.
   PubMed Web of Science ®Google Scholar
• Altunatmaz SS, Issa G, Aydin A. Detection of airborne psychrotrophic bacteria and fungi in food storage refrigerators. Braz J Microbiol. 2012;43(4):1436–1443. doi:10.1590/S1517-83822012000400027.
   PubMed Web of Science ®Google Scholar
• Tournas VH. Spoilage of vegetable crops by bacteria and fungi and related health hazards. Crit Rev Microbiol. 2005;31(1):33–44. doi:10.1080/10408410590886024.
   PubMed Web of Science ®Google Scholar
• Dagenais TR, Keller NP. Pathogenesis of Aspergillus fumigatus in invasive aspergillosis. Clin Microbiol Rev. 2009;22(3):447–465. doi:10.1128/CMR.00055-08.
   PubMed Web of Science ®Google Scholar
• Seroy J, Antiporta P, Grim SA, Proia LA, Singh K, Clark NM. Aspergillus calidoustus case series and review of the literature. Transpl Infect Dis. 2017;19(5):e12755. doi:10.1111/tid.12755.
   Web of Science ®Google Scholar
• Sugui JA, Kwon-Chung KJ, Juvvadi PR, Latge JP, Steinbach WJ. Aspergillus fumigatus and related species. Cold Spring Harb Perspect Med. 2014;5(2):a019786. doi:10.1101/cshperspect.a019786.
   PubMed Web of Science ®Google Scholar
• Nalcacioglu H, Yakupoglu YK, Genc G, et al. Disseminated fungal infection by Aureobasidium pullulans in a renal transplant recipient. Pediatr Transplant. 2018;22(3):e13152. doi:10.1111/petr.13152.
   PubMed Web of Science ®Google Scholar
• Salama AD, Rogers T, Lord GM, Lechler RI, Mason PD. Multiple Cladosporium brain abscesses in a renal transplant patient: aggressive management improves outcome. Transplantation. 1997;63(1):160–162. doi:10.1097/00007890-199701150-00030.
   PubMed Web of Science ®Google Scholar
• Schinabeck MK, Ghannoum MA. Human hyalohyphomycoses: a review of human infections due to Acremonium spp., Paecilomyces spp., Penicillium spp., and Scopulariopsis spp. J Chemother. 2003;15(sup2):5–15. doi:10.1179/joc.2003.15.Supplement-2.5.
   PubMed Web of Science ®Google Scholar
• Bundy KW, Gent JF, Beckett W, et al. Household airborne Penicillium associated with peak expiratory flow variability in asthmatic children. Ann Allergy Asthma Immunol. 2009;103(1):26–30. doi:10.1016/S1081-1206(10)60139-1.
   PubMed Web of Science ®Google Scholar
• Niedoszytko M, Chełmińska M, Jassem E, Czestochowska E. Association between sensitization to Aureobasidium pullulans (Pullularia sp) and severity of asthma. Ann Allergy Asthma Immunol. 2007;98(2):153–156. doi:10.1016/S1081-1206(10)60688-6.
   PubMed Web of Science ®Google Scholar
• Weryszko-Chmielewska E, Kasprzyk I, Nowak M, et al. Health hazards related to conidia of Cladosporium-biological air pollutants in Poland, central Europe. J Environ Sci (China). 2018;65:271–281. doi:10.1016/j.jes.2017.02.018.
   PubMed Web of Science ®Google Scholar
• Chen BY, Jasmine Chao H, Wu CF, Kim H, Honda Y, Guo YL. High ambient Cladosporium spores were associated with reduced lung function in schoolchildren in a longitudinal study. Sci Total Environ. 2014;481:370–376. doi:10.1016/j.scitotenv.2014.01.078.
   PubMed Web of Science ®Google Scholar
• Temprano J, Becker BA, Hutcheson PS, Knutsen AP, Dixit A, Slavin RG. Hypersensitivity pneumonitis secondary to residential exposure to Aureobasidium pullulans in 2 siblings. Ann Allergy Asthma Immunol. 2007;99(6):562–566. doi:10.1016/S1081-1206(10)60387-0.
   PubMed Web of Science ®Google Scholar
• Chiba S, Okada S, Suzuki Y, et al. Cladosporium species-related hypersensitivity pneumonitis in household environments. Intern Med. 2009;48(5):363–367. doi:10.2169/internalmedicine.48.1811.
   PubMed Web of Science ®Google Scholar
• Park HS, Jung KS, Kim SO, Kim SJ. Hypersensitivity pneumonitis induced by Penicillium expansum in a home environment. Clin Exp Allergy. 1994;24(4):383–385. doi:10.1111/j.1365-2222.1994.tb00251.x.
   PubMed Web of Science ®Google Scholar
• Segal BH. Aspergillosis. N Engl J Med. 2009;360(18):1870–1884. doi:10.1056/NEJMra0808853.
   PubMed Web of Science ®Google Scholar
• Leff JW, Fierer N. Bacterial communities associated with the surfaces of fresh fruits and vegetables. PLoS One. 2013;8(3):e59310. doi:10.1371/journal.pone.0059310.
   PubMed Web of Science ®Google Scholar
• Nunes AP, Teixeira LM, Iorio NL, et al. Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening. Int J Antimicrob Agents. 2006;27(4):307–315. doi:10.1016/j.ijantimicag.2005.11.013.
   PubMed Web of Science ®Google Scholar
• Mendoza-Olazaran S, Morfin-Otero R, Villarreal-Trevino L, et al. Antibiotic susceptibility of biofilm cells and molecular characterisation of Staphylococcus hominis isolates from blood. PLoS One. 2015;10(12):e0144684.
   PubMed Web of Science ®Google Scholar
• Cimiotti JP, Haas JP, Della-Latta P, Wu F, Saiman L, Larson EL. Prevalence and clinical relevance of Staphylococcus warneri in the neonatal intensive care unit. Infect Control Hosp Epidemiol. 2007;28(3):326–330. doi:10.1086/511998.
   PubMed Web of Science ®Google Scholar
• Perdreau-Remington F, Stefanik D, Peters G, et al. Methicillin-resistant Staphylococcus haemolyticus on the hands of health care workers: a route of transmission or a source? J Hosp Infect. 1995;31(3):195–203. doi:10.1016/0195-6701(95)90066-7.
   PubMed Web of Science ®Google Scholar