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Original Articles

Sensitive detection of porcine DNA in processed animal proteins using a TaqMan real-time PCR assay

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Pages 1402-1412 | Received 16 Dec 2011, Accepted 20 May 2012, Published online: 10 Jul 2012

References

  • Bellagamba , F , Comincini , S , Ferretti , L , Valfre , F and Moretti , VM . 2006 . Application of quantitative real-time PCR in the detection of prion-protein gene species-specific DNA sequences in animal meals and feedstuffs . J Food Prot , 69 : 891 – 896 .
  • Bottero , MT , Dalmasso , A , Nucera , D , Turi , RM , Rosati , S , Squadrone , S , Goria , M and Civera , T . 2003 . Development of a PCR assay for the detection of animal tissues in ruminant feeds . J Food Prot , 66 : 2307 – 2312 .
  • Bustin , SA , Benes , V , Garson , JA , Hellemans , J , Huggett , J , Kubista , M , Mueller , R , Nolan , T , Pfaffl , MW Shipley , GL . 2009 . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments . Clin Chem , 55 : 611 – 622 .
  • Camacho , MA , Torres , AI , Gil-Alegre , ME , Obregón , MM and Ruz , V . 1993 . Validation protocol of analytical methods for finished pharmaceutical products . STP Pharma Pratiques , 3 : 197 – 202 .
  • Cawthraw , S , Saunders , GC , Martin , TC , Sawyer , J , Windl , O and Readney , SD . 2009 . Real-time PCR detection and identification of prohibited mammalian and avian material in animal feeds . J Food Prot , 72 : 1055 – 1062 .
  • Colgan , S , O’Brien , L , Maher , M , Shilton , N , McDonnell , K and Ward , S . 2001 . Development of a DNA-based assay for species identification in meat and bone meal . Food Res Int , 34 : 409 – 414 .
  • Collins , MJ , Nielsen-Marsh , CM , Hiller , J , Smith , CI , Roberts , JP , Prigodich , RV , Wess , TJ , Csapò , J , Millard , AR and Turner-Walker , G . 2002 . The survival of organic matter in bone: a review . Archaeometry , 44 : 383 – 394 .
  • Dalmasso , A , Fontanella , E , Piatti , P , Civera , T , Rosati , S and Bottero , MT . 2004 . A multiplex PCR assay for the identification of animal species in feedstuffs . Mol Cell Probes , 18 : 81 – 87 .
  • EFSA Panel on Biological Hazards . (BIOHAZ). 2011. Scientific opinion on the revision of the quantitative risk assessment (QRA) of the BSE risk posed by processed animal proteins (PAPs). EFSA Journal. 9:1947–2026
  • European Commission . 2003 . Commission Regulation 2003/1234/EC of 10 July 2003 amending annexes I, IV and XI to Regulation 2001/999/EC of the European Parliament and of the Council and Regulation 2000/11326/EC as regards transmissible spongiform encephalopathies and animal feeding . Off J Eur Comm , L173 : 6 – 13 .
  • European Commission . 2009a . Commission Regulation 2009/1069/EC of 21 October 2009 laying down laying down health rules as regards animal by-products and derived products not intended for human consumption and repealing Regulation (EC) No 1774/2002 (Animal by-products Regulation) . Off J Eur Comm , L300 : 1 – 33 .
  • European Commission . 2009b . Commission Regulation 2009/152/EC of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed . Off J Eur Comm , L54 : 1 – 130 .
  • Frezza , D , Giambra , V , Chegdani , F , Fontana , C , Maccabiani , G , Losio , N , Faggionato , E , Chiappini , B , Vaccari , G von Holst , C . 2008 . Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs . Innov Food Sci Emerg Technol , 9 : 18 – 23 .
  • Fumière , O , Dubois , M , Baeten , V , von Holst , C and Berben , G . 2006 . Effective PCR detection of animal species in highly processed animal byproducts and compound feeds . Anal Bioanal Chem , 385 : 1045 – 1054 .
  • Fumière , O , Marien , A , Fernández Pierna , JA , Baeten , V and Berben , G . 2010 . Development of a real-time PCR protocol for the species origin confirmation of isolated animal particles detected by NIRM . Food Addit Contam A , 27 : 1118 – 1127 .
  • Fumière , O , Veys , P , Boix , A , von Holst , C , Baeten , V and Berben , G . 2009 . Methods of detection, species identification and quantification of processed animal proteins in feedingstuffs . Biotechnol Agron Soc Environ , 13 : 59 – 70 .
  • Gašparič , MB , Tengs , T , la Paz , JL , Holst-Jensen , A , Pla , M , Esteve , M , Žel , J and Gruden , K . 2010 . Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection . Anal Bioanal Chem , 396 : 2023 – 2029 .
  • Gizzi , G , van Raamsdonk , LWD , Baeten , V , Murray , I , Berben , G , Brambilla , G and von Holst , C . 2003 . An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy . Rev Off Int Epizoot , 22 : 311 – 331 .
  • Götherström , A , Collins , MJ , Angerbjörn , A and Lidén , K . 2002 . Bone preservation and DNA amplification . Archaeometry , 44 : 395 – 404 .
  • Gryson , N . 2010 . Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review . Anal Bioanal Chem , 396 : 2003 – 2022 .
  • Hahn , H . 1999 . Animal meal: production and determination in feedstuffs and the origin of bovine spongiform encephalopathy . Naturwissenschaften , 86 : 62 – 70 .
  • International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH). 2005. Harmonised tripartite guideline. Validation of analytical procedures: text and methodology Q2 (R1); [cited 2011 Dec]. Available from: http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1_Guideline.pdf/
  • Kubota , K , Ohashi , A , Imachi , H and Harada , H . 2006 . Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes . Appl Environ Microbiol , 72 : 5311 – 5317 .
  • Martín , I , García , T , Fajardo , V , Rojas , M , Pegels , N , Hernández , PE , González , I and Martín , R . 2009 . SYBR-green real-time PCR approach for the detection and quantification of pig DNA in feedstuffs . Meat Sci , 82 : 252 – 259 .
  • Mendoza-Romero , L , Verkaar , ELC , Savelkoul , PH , Catsburg , A , Aarts , HJM , Buntjer , JB and Lenstra , L . 2004 . Real-time PCR detection of ruminant DNA . J Food Prot , 67 : 550 – 554 .
  • Momcilovic , D and Rasooly , A . 2000 . Detection and analysis of animal materials in food and feed . J Food Prot , 63 : 1602 – 1609 .
  • Pegels , N , González , I , Martín , I , Rojas , M , García , T and Martín , R . 2011 . Applicability assessment of a real-time PCR assay for the specific detection of bovine, ovine and caprine material in feedstuffs . Food Control , 22 : 1189 – 1196 .
  • Prado , M , Berben , G , Fumière , O , van Duijn , G , Mensinga-Kruize , J , Reaney , S , Boix , A and von Holst , C . 2007 . Detection of ruminant meat and bone meals in animal feed by real-time polymerase chain reaction: result of an interlaboratory study . J Agric Food Chem , 55 : 7495 – 7501 .
  • Prado , M , Fumière , O , Boix , A , Marien , A , Berben , G and von Holst , C . 2009 . Novel approach for interlaboratory transfer of real-time PCR methods: detecting bovine meat and bone meal in feed . Anal Bioanal Chem , 394 : 1423 – 1431 .
  • Rojas , M , González , I , Pavón , MA , Pegels , N , Hernández , PE , García , T and Martín , R . 2011 . Application of a real-time PCR assay for the detection of ostrich (Struthio camelus) mislabelling in meat products from the retail market . Food Control , 22 : 523 – 531 .
  • Tajima , K , Osamu , E , Amari , M , Mitsumori , M , Kajikawa , H , Kurihara , M , Yanai , S , Matsui , H , Yasue , H Mitsuhashi , T . 2002 . PCR detection of DNAs of animal origin in feed by primers based on sequences of short and long interspersed repetitive elements . Biosci Biotechnol Biochem , 66 : 2247 – 2250 .
  • van Raamsdonk , L , Pinotti , L , Veys , P , Bremer , M , Hekman , W , Kemmers , A , Campagnoli , A , Paltanin , C , Belinchón-Crespo , C Vliege , J . 2011 . New developments in classical microscopy; what can be expected for the official control? . Biotechnol Agron Soc Environ , 15 : 15 – 24 .
  • Yancy , HF , Washington , JD , Callahan , L , Mason , JA , Deaver , CM , Farrell , DE , Ha , T , Sespico , E , Falmlen , D and Myers , MJ . 2009 . Development, evaluation, and peer verification of a rapid real-time PCR method for the detection of animal material . J Food Prot , 72 : 2368 – 2374 .
  • Yoshida , T , Nomura , T , Shinoda , N , Kusama , T , Kadowaki , K and Katsuaki , S . 2009 . Development of PCR primers for the detection of porcine DNA in feed using mtATP6 as the target sequence . Shokuhin Eiseigaku Zasshi , 50 : 89 – 92 .

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