Detection by real-time PCR and pyrosequencing of the cry1Ab and cry1Ac genes introduced in genetically modified (GM) constructs

References

• AFNOR XP V03-020-2. 2008. Produits alimentaires. Détection et quantification des organismes végétaux génétiquement modifiés et produits dérivés. Partie 2: méthodes basées sur la réaction de polymérisation en chaîne. Norme expérimentale.
   Google Scholar
• Arumuganathan K, Earle ED. 1991. Nuclear DNA content of some important plant species. Plant Mol Biol Rep. 9:208–218.
   Google Scholar
• Barbau-Piednoir E, Lievens A, Vandermassen E, Mbongolo-Mbella EG, Leunda-Casi A, Roosens N, Sneyers M, Van den Bulcke M. 2012. Four new SYBR® Green qPCR screening methods for the detection of Roundup Ready®, LibertyLink®, and CryIAb traits in genetically modified products. Eur Food Res Technol. 234:13–23.
   Web of Science ®Google Scholar
• Bennett MD, Leitch IJ. 2005. Nuclear DNA amounts in angiosperms: progress, problems and prospects. Ann Bot. 95:45–90.
   PubMed Web of Science ®Google Scholar
• Broeders S, Huber I, Grohmann L, Berben G, Taverniers I, Mazzara M, Roosens N, Morisset D. 2014. Guidelines for validation of qualitative real-time PCR methods. Trends Food Sci Technol. 37:115–126.
   Web of Science ®Google Scholar
• Bruderer S, Ke L. 2003. Genetically Modified (GM) crops: molecular and regulatory details. 2003, BATS, Centre for biosafety and sustainability. [ cited 2016 Nov 17]. Available from: http://www.bats.ch/gmo-watch/GVO-report140703.pdf
   Google Scholar
• Center for Environmental Risk Assessment (CERA): GM Crop Database. [ Cited 2016 Nov 17]. Available from: http://cera-gmc.org/index.php?action=gm_crop_database
   Google Scholar
• Choi SH. 2011. Hexaplex PCR assay and liquid bead array for detection of stacked genetically modified cotton event 281-24-236x3006-210-23. Anal Bioanal Chem. 401:647–655.
   PubMed Web of Science ®Google Scholar
• Codex Alimentarius. 2010. Guidelines on performance criteria and validation of methods for detection, identification and quantification of specific DNA sequences and specific proteins in foods. CAC/GL 74-2010. [ cited 2016 Jan 21]. Available from: http://www.codexalimentarius.org/standards/list-of-standards/
   Google Scholar
• Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van Rie J, Lereclus D, Baum J, Dean DH. 1998. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol Mol Biol Rev. 62:807–813.
   PubMed Web of Science ®Google Scholar
• Debode F, Huber I, Macarthur R, Rischitor PE, Mazzara M, Herau V, Sebah D, Dobnik D, Broeders S, Roosens NH, et al. 2017a. Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection. Food Control. 73:452–461.
   Web of Science ®Google Scholar
• Debode F, Janssen E, Arranz E, Roulez D, Ancion C, Antoine G, Hulin J, Berben G. 2009. Detection of Bacillus thuringiensis. Co-Extra. International conference; Paris. Book of abstracts, abstract P6. p. 103–104. [ cited 2016 Oct 26]. Avaialble from: http://www.bch.cbd.int/database/attachment/?id=10231
   Google Scholar
• Debode F, Janssen E, Berben G. 2007. Physical degradation of genomic DNA of soybean flours does not impair relative quantification of its transgenic content. Eur Food Res Technol. 226:273–280.
   Web of Science ®Google Scholar
• Debode F, Janssen E, Berben G. 2013. Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7). Eur Food Res Technol. 236:659–669.
   Web of Science ®Google Scholar
• Debode F, Janssen EBerben G. 2017c. Development of screening PCR assays focused on the gox, gus, EPSPS, pat and bar genes for GMO detection. Submitted for publication.
   Google Scholar
• Debode F, Janssen E, Marien A, Berben G. 2012. DNA detection by conventional and real-time PCR after extraction from vegetable oils. J Am Oil Chemists’ Soc. 89:1249–1257.
   Web of Science ®Google Scholar
• Debode F, Marien A, Janssen E, Bragard C, Berben G. 2017b. Influence of the amplicon length on real-time PCR results. Biotechnologie, Agronomie, Société et Environnement. 21:3–11.
   Web of Science ®Google Scholar
• Dinon AZ, Prins TW, van Dijk JP, Arisi ACM, Scholtens IM, Kok EJ. 2011. Development and validation of real-time PCR screening methods for detection of cry1A. 105 and cry2Ab2 genes in genetically modified organisms. Anal Bioanal Chem. 400:1433–1442.
   PubMed Web of Science ®Google Scholar
• Doležel J, Greilhuber J. 2010. Nuclear genome size: are we getting closer? Cytometry Part. 77:635–642.
   PubMed Web of Science ®Google Scholar
• Ehlers B, Strauch E, Goltz M, Kubsch D, Wagner H, Maidhof H, Bendiek J, Appe L, Buhk HJ. 1997. Nachweis gentechnischer Veränderungen in Mais mittels PCR. Bundesgesundheitsblatt. 40:118–121.
   Google Scholar
• EURL GMFF. 2014. Revised guidance for testing GM rice originating from China JRC Publication JRC90142. Avaialble from: http://gmo-crl.jrc.ec.europa.eu/doc/EURL-MV-01-11.pdf
   Google Scholar
• Fakruddin M, Chowdhury A, Hossain M, Mannan K, Mazumda R. 2012. Pyrosequencing-principles and applications. Int J Life Sci Pharma Res. 2:65–76.
   Google Scholar
• Genetic Rights Foundation. Biosafety scanner. [ cited 2016 Nov 17]. Available form: http://en.biosafetyscanner.org
   Google Scholar
• Germini A, Zanetti A, Salati C, Rossi S, Forré C, Schmid S, Marchelli R. 2004. Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. J Agric Food Chem. 52:3275–3280.
   PubMed Web of Science ®Google Scholar
• Grohmann L, Reiting R, Mäde D, Uhlig S, Simon K, Frost K, Randhawa GJ, Zur K. 2015. Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products. Accreditation Qual Assur. 20:85–96.
   Web of Science ®Google Scholar
• Guan X, Guo J, Shen P, Yang L, Zhang D. 2010. Visual and rapid detection of two genetically modified soybean events using loop-mediated isothermal amplification method. Food Anal Methods. 3:313–320.
   Web of Science ®Google Scholar
• Guertler P, Huber I, Pecoraro S, Busch U. 2012. Development of an event-specific detection method for genetically modified rice Kefeng 6 by quantitative real-time PCR. Journal für Verbraucherschutz und Lebensmittelsicherheit. 7:63–70.
   Web of Science ®Google Scholar
• Höfte H, Whiteley HR. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev. 53:242–255.
   PubMedGoogle Scholar
• ISO 21571. 2005. Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Nucleic acid extraction. International Organization for Standardization. Geneva.
   Google Scholar
• Kamle S, Kumar A, Bhatnagar RK. 2011. Development of multiplex and construct specific PCR assay for detection of cry2Ab transgene in genetically modified crops and product. GM Crops. 2:74–81.
   PubMedGoogle Scholar
• Maciel H, Zingaretti S, Maciel G. 2014. Comparative analysis of the amino acid sequence of the cry protein of Bacillus thuringiensis codify the toxic protein to insects of the orders Lepidoptera, Diptera and Lepidoptera/Diptera. In: BMC Proceedings. Vols. 8, No. Suppl 4. BioMed Central Ltd; p. P120. doi:10.1186/1753-6561-8-S4-P120
   Google Scholar
• Matsuoka T, Kuribara H, Takubo K, Akiyama H, Miura H, Goda Y, Kusakabe Y, Isshiki K, Toyoda M, Hino A. 2002. Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays). J Agric Food Chem. 50:2100–2109.
   PubMed Web of Science ®Google Scholar
• Permingeat HR, Reggiardo MI, Vallejos RH. 2002. Detection and quantification of transgenes in grains by multiplex and real-time PCR. J Agric Food Chem. 50:4431–4436.
   PubMed Web of Science ®Google Scholar
• Praca-Fontes MM, Carvalho CR, Clarindo WR, Cruz CD. 2011. Revisiting the DNA C-values of the genome size-standards used in plant flow cytometry to choose the “best primary standards”. Plant Cell Rep. 30:1183–1191.
   PubMed Web of Science ®Google Scholar
• Reiting R, Grohmann L, Moris G, Mäde D. 2013. Detection and characterization of an unknown rice event in Basmati rice products. Eur Food Res Technol. 236:715–723.
   Web of Science ®Google Scholar
• Rho JK, Lee T, Jung SI, Kim TS, Park YH, Kim YM. 2004. Qualitative and quantitative PCR methods for detection of three lines of genetically modified potatoes. J Agric Food Chem. 52:3269–3274.
   PubMed Web of Science ®Google Scholar
• Ronaghi M, Uhlén M, Nyren P. 1998. A sequencing method based on real-time pyrophosphate. Science. 281:363.
   PubMed Web of Science ®Google Scholar
• Stave JW. 2002. Protein immunoassay methods for detection of biotech crops: applications, limitations, and practical considerations. J AOAC Int. 85:780–786.
   PubMed Web of Science ®Google Scholar
• Strizhov N, Keller M, Mathur J, Koncz-Kalman Z, Bosch D, Prudovsky E, Schell J, Sneh B, Koncz C, Zilberstein A. 1996. A synthetic cryIC gene, encoding a Bacillus thuringiensis δ-endotoxin, confers Spodoptera resistance in alfalfa and tobacco. PNAS. 93:15012–15017.
   PubMed Web of Science ®Google Scholar
• Trapmann S, Burns M, Broll H, Macarthur R, Wood RKS, Žel J. 2007. Guidance document on measurement uncertainty for GMO testing laboratories. EUR Report 22756 EN. Office for official publications of the Luxemburg: European Communities. ISBN 978-92-79-05566-9.
   Google Scholar
• Vaïtilingom M, Pijnenburg H, Gendre F, Brignon P. 1999. Real-time quantitative PCR detection of genetically modified maximizer maize and roundup ready soybean in some representative foods. J Agric Food Chem. 47:5261–5266.
   PubMed Web of Science ®Google Scholar
• Van den Bulcke M, De Schrijver A, De Bernardi D, Devos Y, MbongoMbella G, Casi AL, Moens W, Sneyers M. 2007. Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples. Eur Food Res Technol. 225:49–57.
   Web of Science ®Google Scholar
• Vollenhofer S, Burg K, Schmidt J, Kroath H. 1999. Genetically modified organisms in food screening and specific detection by polymerase chain reaction. J Agric Food Chem. 47:5038–5043.
   PubMed Web of Science ®Google Scholar
• Windels P, Bertrand S, Depicker A, Moens W, Van Bockstaele E, De Loose M. 2003. Qualitative and event-specific PCR real-time detection methods for StarLink maize. Eur Food Res Technol. 216:259–263.
   Web of Science ®Google Scholar