ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus

References

• Daniels NA, MacKinnon L, Bishop R, Altekruse S, Ray B, Hammond RM, Thompson S, Wilson S, Bean NH, Griffin PM, et al. Vibrio parahaemolyticus infections in the United States, 1973-1998. J Infect Dis. 2000;181(5):1661–20. doi:10.1086/315459.
   PubMed Web of Science ®Google Scholar
• McLaughlin JB, DePaola A, Bopp CA, Martinek KA, Napolilli NP, Allison CG, Murray SL, Thompson EC, Bird MM, Middaugh JP. Outbreak of Vibrio parahaemolyticus gastroenteritis associated with Alaskan oysters. N Engl J Med. 2005;353(14):1463–1470. doi:10.1056/NEJMoa051594.
   PubMed Web of Science ®Google Scholar
• Su YC, Liu C. Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol. 2007;24(6):549–558. doi:10.1016/j.fm.2007.01.005.
   PubMed Web of Science ®Google Scholar
• Makino K, Oshima K, Kurokawa K, Yokoyama K, Uda T, Tagomori K, Iijima Y, Najima M, Nakano M, Yamashita A, et al. Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V cholerae. Lancet. 2003;361(9359):743–749. English. doi:10.1016/S0140-6736(03)12659-1.
   PubMed Web of Science ®Google Scholar
• de Souza Santos M, Orth K, Miller JF. Intracellular Vibrio parahaemolyticus escapes the vacuole and establishes a replicative niche in the cytosol of epithelial cells. mBio. 2014;5(5):e01506–01514. doi:10.1128/mBio.01506-14.
   PubMed Web of Science ®Google Scholar
• Piñeyro P, Zhou X, Orfe LH, Friel PJ, Lahmers K, Call DR. Development of two animal models to study the function of Vibrio parahaemolyticus type III secretion systems. Infect Immun. 2010;78(11):4551–4559. doi:10.1128/IAI.00461-10.
   PubMed Web of Science ®Google Scholar
• Hiyoshi H, Kodama T, Iida T, Honda T. Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity, enterotoxicity, and lethality in mice. Infect Immun. 2010;78(4):1772–1780. doi:10.1128/IAI.01051-09.
   PubMed Web of Science ®Google Scholar
• Ono T, Park KS, Ueta M, Iida T, Honda T. Identification of proteins secreted via Vibrio parahaemolyticus type III secretion system 1. Infect Immun. 2006;74(2):1032–1042. doi:10.1128/IAI.74.2.1032-1042.2006.
   PubMed Web of Science ®Google Scholar
• Hueck CJ. Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev. 1998;62(2):379–433. doi:10.1128/MMBR.62.2.379-433.1998.
   PubMed Web of Science ®Google Scholar
• Zhou X, Shah DH, Konkel ME, Call DR. Type III secretion system 1 genes in Vibrio parahaemolyticus are positively regulated by ExsA and negatively regulated by ExsD. Mol Microbiol. 2008;69(3):747–764. doi:10.1111/j.1365-2958.2008.06326.x.
   PubMed Web of Science ®Google Scholar
• Zhou X, Konkel ME, Call DR. Regulation of type III secretion system 1 gene expression in Vibrio parahaemolyticus is dependent on interactions between ExsA, ExsC, and ExsD. Virulence. 2010;1(4):260–272. doi:10.4161/viru.1.4.12318.
   PubMed Web of Science ®Google Scholar
• Kodama T, Yamazaki C, Park KS, Akeda Y, Iida T, Honda T. Transcription of Vibrio parahaemolyticus T3SS1 genes is regulated by a dual regulation system consisting of the ExsACDE regulatory cascade and H-NS. FEMS Microbiol Lett. 2010;311(1):10–17. doi:10.1111/j.1574-6968.2010.02066.x.
   PubMed Web of Science ®Google Scholar
• Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol. 2001;55(1):165–199. doi:10.1146/annurev.micro.55.1.165.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Qiu Y, Tan Y, Guo Z, Yang R, Zhou D, Mukhopadhyay P. Transcriptional regulation of opaR, qrr2–4 and aphA by the Master quorum-sensing regulator OpaR in Vibrio parahaemolyticus. PloS One. 2012;7(4):e34622. doi:10.1371/journal.pone.0034622.
   PubMed Web of Science ®Google Scholar
• Ng WL, Bassler BL. Bacterial quorum-sensing network architectures. Annu Rev Genet. 2009;43(1):197–222. doi:10.1146/annurev-genet-102108-134304.
   PubMed Web of Science ®Google Scholar
• Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011;193(16):4224–4237. doi:10.1128/JB.00432-11.
   PubMed Web of Science ®Google Scholar
• Sun F, Zhang Y, Wang L, Yan X, Tan Y, Guo Z, Qiu J, Yang R, Xia P, Zhou D, et al. Molecular characterization of direct target genes and cis-acting consensus recognized by quorum-sensing regulator AphA in Vibrio parahaemolyticus. PloS One. 2012;7(9):e44210. doi:10.1371/journal.pone.0044210.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Hu L, Qiu Y, Osei-Adjei G, Tang H, Zhang Y, Zhang R, Sheng X, Xu S, Yang W, et al. QsvR integrates into quorum sensing circuit to control Vibrio parahaemolyticus virulence. Environ Microbiol. 2019;21(3):1054–1067. doi:10.1111/1462-2920.14524.
   PubMed Web of Science ®Google Scholar
• Milton DL. Quorum sensing in vibrios: complexity for diversification. Int J Med Microbiol. 2006;296(2–3):61–71. doi:10.1016/j.ijmm.2006.01.044.
   PubMed Web of Science ®Google Scholar
• Henke JM, Bassler BL. Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi. J Bacteriol. 2004c;186(20):6902–6914. English. doi: 10.1128/JB.186.20.6902-6914.2004.
   PubMed Web of Science ®Google Scholar
• Henke JM, Bassler BL. Quorum Sensing Regulates Type III Secretion in Vibrio harveyi and Vibrio parahaemolyticus. J Bacteriol. 2004a;186(12):3794–3805. English. doi: 10.1128/JB.186.12.3794-3805.2004.
   PubMed Web of Science ®Google Scholar
• Lenz DH, Mok KC, Lilley BN, Kulkarni RV, Wingreen NS, Bassler BL. The small RNA chaperone hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae. Cell. 2004;118(1):69–82. English. doi: 10.1016/j.cell.2004.06.009.
   PubMed Web of Science ®Google Scholar
• Tu KC, Bassler BL. Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi. Genes Dev. 2007;21(2):221–233. doi:10.1101/gad.1502407.
   PubMed Web of Science ®Google Scholar
• van Kessel JC, Rutherford ST, Shao Y, Utria AF, Bassler BL. Individual and combined roles of the master regulators AphA and LuxR in control of the Vibrio harveyi quorum-sensing regulon. J Bacteriol. 2013;195(3):436–443. doi:10.1128/JB.01998-12.
   PubMed Web of Science ®Google Scholar
• Chen X, Schauder S, Potier N, Van Dorsselaer A, Pelczer I, Bassler BL, Hughson FM. Structural identification of a bacterial quorum-sensing signal containing boron. Nature. 2002;415(6871):545–549. doi:10.1038/415545a.
   PubMed Web of Science ®Google Scholar
• Croxatto A, Pride J, Hardman A, Williams P, Cámara M, Milton DL. A distinctive dual-channel quorum-sensing system operates in Vibrio anguillarum. Mol Microbiol. 2004;52(6):1677–1689. doi:10.1111/j.1365-2958.2004.04083.x.
   PubMed Web of Science ®Google Scholar
• Gilson L, Kuo A, Dunlap PV. AinS and a new family of autoinducer synthesis proteins. J Bacteriol. 1995;177(23):6946–6951. doi:10.1128/jb.177.23.6946-6951.1995.
   PubMed Web of Science ®Google Scholar
• Henke JM, Bassler BL. Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi. J Bacteriol. 2004b;186(20):6902–6914. doi:10.1128/JB.186.20.6902-6914.2004.
   PubMed Web of Science ®Google Scholar
• Kawase T, Miyoshi S, Sultan Z, Shinoda S. Regulation system for protease production in Vibrio vulnificus. FEMS Microbiol Lett. 2004;240(1):55–59. doi:10.1016/j.femsle.2004.09.023.
   PubMed Web of Science ®Google Scholar
• Miller MB, Skorupski K, Lenz DH, Taylor RK, Bassler BL. Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae. Cell. 2002;110(3):303–314. doi:10.1016/S0092-8674(02)00829-2.
   PubMed Web of Science ®Google Scholar
• Ruby EG, Urbanowski M, Campbell J, Dunn A, Faini M, Gunsalus R, Lostroh P, Lupp C, McCann J, Millikan D, et al. Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners. Proc Natl Acad Sci USA. 2005;102(8):3004–3009. doi:10.1073/pnas.0409900102.
   PubMed Web of Science ®Google Scholar
• Liu AC, Thomas NA. Transcriptional profiling of Vibrio parahaemolyticus exsA reveals a complex activation network for type III secretion. Front Microbiol. 2015;6:1089. doi:10.3389/fmicb.2015.01089.
   PubMed Web of Science ®Google Scholar
• Alvarez AF, Georgellis D. In vitro and in vivo analysis of the ArcB/A redox signaling pathway. Methods Enzymol. 2010;471:205–228.
   PubMed Web of Science ®Google Scholar
• Iuchi S, Chepuri V, Fu HA, Gennis RB, Lin EC. Requirement for terminal cytochromes in generation of the aerobic signal for the arc regulatory system in Escherichia coli: study utilizing deletions and lac fusions of cyo and cyd. J Bacteriol. 1990;172(10):6020–6025. doi:10.1128/jb.172.10.6020-6025.1990.
   PubMed Web of Science ®Google Scholar
• Georgellis D, Kwon O, Lin EC. Quinones as the redox signal for the arc two-component system of bacteria. Sci. 2001;292(5525):2314–2316. doi:10.1126/science.1059361.
   PubMed Web of Science ®Google Scholar
• Kwon O, Georgellis D, Lin EC. Phosphorelay as the sole physiological route of signal transmission by the arc two-component system of Escherichia coli. J Bacteriol. 2000;182(13):3858–3862. doi:10.1128/JB.182.13.3858-3862.2000.
   PubMed Web of Science ®Google Scholar
• Rolfe MD, Ter Beek A, Graham AI, Trotter EW, Asif HM, Sanguinetti G, de Matto JT, Poole RK, Green J, de Mattos JT. Transcript profiling and inference of Escherichia coli K-12 ArcA activity across the range of physiologically relevant oxygen concentrations. J Biol Chem. 2011;286(12):10147–10154. doi:10.1074/jbc.M110.211144.
   PubMed Web of Science ®Google Scholar
• Bose JL, Kim U, Bartkowski W, Gunsalus RP, Overley AM, Lyell NL, Visick KL, Stabb EV. Bioluminescence in Vibrio fischeri is controlled by the redox-responsive regulator ArcA. Mol Microbiol. 2007;65(2):538–553. doi:10.1111/j.1365-2958.2007.05809.x.
   PubMed Web of Science ®Google Scholar
• Giaouris E, Samoilis G, Chorianopoulos N, Ercolini D, Nychas GJ. Differential protein expression patterns between planktonic and biofilm cells of Salmonella enterica serovar Enteritidis PT4 on stainless steel surface. Int J Food Microbiol. 2013;162(1):105–113. English. doi: 10.1016/j.ijfoodmicro.2012.12.023.
   PubMed Web of Science ®Google Scholar
• Gunsalus RP, Park SJ. Aerobic-anaerobic gene regulation in Escherichia coli: control by the ArcAB and fnr regulons. Res Microbiol. 1994;145(5–6):437–450. doi:10.1016/0923-2508(94)90092-2.
   PubMed Web of Science ®Google Scholar
• Lee YS, Han JS, Jeon Y, Hwang DS. The arc two-component signal transduction system inhibits in vitro Escherichia coli chromosomal initiation. J Biol Chem. 2001;276(13):9917–9923. doi:10.1074/jbc.M008629200.
   PubMed Web of Science ®Google Scholar
• Longo P, Ota-Tsuzuki C, Nunes A, Fernandes B, Mintz K, Fives-Taylor P, Mayer M. Aggregatibacter actinomycetemcomitans arcB influences hydrophobic properties, biofilm formation and adhesion to hydroxyapatite. Braz J Microbiol. 2009;40(3):550–562. doi:10.1590/S1517-83822009000300018.
   PubMed Web of Science ®Google Scholar
• Wang Z, Sun J, Xia T, Liu Y, Fu J, Lo YK, Chang C, Yan A, Liu X. Proteomic Delineation of the ArcA Regulon in Salmonella Typhimurium During Anaerobiosis. Molecular & Cellular Proteomics: MCP. 2018;17(10):1937–1947. doi:10.1074/mcp.RA117.000563.
   PubMed Web of Science ®Google Scholar
• Jiang L, Feng L, Yang B, Zhang W, Wang P, Jiang X, Wang L, Slauch JM. Signal transduction pathway mediated by the novel regulator LoiA for low oxygen tension induced Salmonella Typhimurium invasion. PLoS Pathog. 2017;13(6):e1006429. doi:10.1371/journal.ppat.1006429.
   PubMed Web of Science ®Google Scholar
• Sengupta N, Paul K, Chowdhury R. The global regulator ArcA modulates expression of virulence factors in Vibrio cholerae. Infect Immun. 2003;71(10):5583–5589. doi:10.1128/IAI.71.10.5583-5589.2003.
   PubMed Web of Science ®Google Scholar
• Appleby JL, Parkinson JS, Bourret RB. Signal transduction via the multi-step phosphorelay: not necessarily a road less traveled. Cell. 1996;86(6):845–848. doi:10.1016/S0092-8674(00)80158-0.
   PubMed Web of Science ®Google Scholar
• Ishige K, Nagasawa S, Tokishita S, Mizuno T. A novel device of bacterial signal transducers. EMBO J. 1994;13(21):5195–5202. doi:10.1002/j.1460-2075.1994.tb06850.x.
   PubMed Web of Science ®Google Scholar
• Matsubara M, Kitaoka SI, Takeda SI, Mizuno T. Tuning of the porin expression under anaerobic growth conditions by his-to-Asp cross-phosphorelay through both the EnvZ-osmosensor and ArcB-anaerosensor in Escherichia coli. Genes To Cells. 2000;5(7):555–569. doi:10.1046/j.1365-2443.2000.00347.x.
   PubMed Web of Science ®Google Scholar
• Perraud AL, Weiss V, Gross R. Signalling pathways in two-component phosphorelay systems. Trends Microbiol. 1999;7(3):115–120. doi:10.1016/S0966-842X(99)01458-4.
   PubMed Web of Science ®Google Scholar
• Yaku H, Kato M, Hakoshima T, Tsuzuki M, Mizuno T. Interaction between the CheY response regulator and the histidine-containing phosphotransfer (HPt) domain of the ArcB sensory kinase in Escherichia coli. FEBS Lett. 1997;408(3):337–340. doi:10.1016/S0014-5793(97)00459-6.
   PubMed Web of Science ®Google Scholar
• Milton DL, O’Toole R, Horstedt P, Wolf-Watz H. Flagellin a is essential for the virulence of Vibrio anguillarum. J Bacteriol. 1996;178(5):1310–1319. doi:10.1128/jb.178.5.1310-1319.1996.
   PubMed Web of Science ®Google Scholar
• Morales VM, Bäckman A, Bagdasarian M. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene. 1991;97(1):39–47. doi:10.1016/0378-1119(91)90007-X.
   PubMed Web of Science ®Google Scholar
• Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM 2nd, Peterson KM. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene. 1995;166(1):175–176. doi:10.1016/0378-1119(95)00584-1.
   PubMed Web of Science ®Google Scholar
• Qing G, Ma LC, Khorchid A, Swapna GV, Mal TK, Takayama MM, Xia B, Phadtare S, Ke H, Acton T, et al. Cold-shock induced high-yield protein production in Escherichia coli. Nat Biotechnol. 2004;22(7):877–882. doi:10.1038/nbt984.
   PubMed Web of Science ®Google Scholar
• Park KS, Ono T, Rokuda M, Jang MH, Okada K, Iida T, Honda T. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect Immun. 2004;72(11):6659–6665. doi:10.1128/IAI.72.11.6659-6665.2004.
   PubMed Web of Science ®Google Scholar
• Kinoshita E, Kinoshita-Kikuta E, Shiba A, Edahiro K, Inoue Y, Yamamoto K, Yoshida M, Koike T. Profiling of protein thiophosphorylation by Phos-tag affinity electrophoresis: evaluation of adenosine 5’-O-(3-thiotriphosphate) as a phosphoryl donor in protein kinase reactions. Proteomics. 2014;14(6):668–679. doi:10.1002/pmic.201300533.
   PubMed Web of Science ®Google Scholar
• Liang X, Moore R, Wilton M, Wong MJ, Lam L, Dong TG. Identification of divergent type VI secretion effectors using a conserved chaperone domain. Proc Natl Acad Sci USA. 2015;112(29):9106–9111. doi:10.1073/pnas.1505317112.
   PubMed Web of Science ®Google Scholar
• Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 2001;25(4):402–408. doi:10.1006/meth.2001.1262.
   PubMed Web of Science ®Google Scholar
• Liu M, Zhu X, Zhang C, Zhao Z. LuxQ-LuxU-LuxO pathway regulates biofilm formation by Vibrio parahaemolyticus. Microbiol Res. 2021;250:126791. doi:10.1016/j.micres.2021.126791.
   PubMedGoogle Scholar
• Freeman JA, Bassler BL. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. J Bacteriol. 1999b;181(3):899–906. doi:10.1128/JB.181.3.899-906.1999.
   PubMed Web of Science ®Google Scholar
• Gao R, Stock AM. Biological insights from structures of two-component proteins. Annu Rev Microbiol. 2009;63(1):133–154. doi:10.1146/annurev.micro.091208.073214.
   PubMedGoogle Scholar
• Freeman JA, Bassler BL. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. Mol Microbiol. 1999a;31(2):665–677. doi:10.1046/j.1365-2958.1999.01208.x.
   PubMed Web of Science ®Google Scholar
• Iuchi S, Lin EC. arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways. Proc Natl Acad Sci USA. 1988;85(6):1888–1892. doi:10.1073/pnas.85.6.1888.
   PubMed Web of Science ®Google Scholar
• Shimohata T, Mawatari K, Iba H, Hamano M, Negoro S, Asada S, Aihara M, Hirata A, Su Z, Takahashi A. VopB1 and VopD1 are essential for translocation of type III secretion system 1 effectors of Vibrio parahaemolyticus. Can J Microbiol. 2012;58(8):1002–1007. doi:10.1139/w2012-081.
   PubMed Web of Science ®Google Scholar
• Burdette DL, Seemann J, Orth K. Vibrio VopQ induces PI3-kinase-independent autophagy and antagonizes phagocytosis. Mol Microbiol. 2009;73(4):639–649. doi:10.1111/j.1365-2958.2009.06798.x.
   PubMed Web of Science ®Google Scholar
• Mizuno T. His-Asp phosphotransfer signal transduction. J Biochem. 1998;123(4):555–563. doi:10.1093/oxfordjournals.jbchem.a021972.
   PubMed Web of Science ®Google Scholar
• Matsushika A, Mizuno T. A dual-signaling mechanism mediated by the ArcB hybrid sensor kinase containing the histidine-containing phosphotransfer domain in Escherichia coli. J Bacteriol. 1998;180(15):3973–3977. doi:10.1128/JB.180.15.3973-3977.1998.
   PubMed Web of Science ®Google Scholar
• Fong KP, Gao L, Demuth DR. luxS and arcB control aerobic growth of Actinobacillus actinomycetemcomitans under iron limitation. Infect Immun. 2003;71(1):298–308. doi:10.1128/IAI.71.1.298-308.2003.
   PubMed Web of Science ®Google Scholar
• Salmon KA, Hung SP, Steffen NR, Krupp R, Baldi P, Hatfield GW, Gunsalus RP. Global gene expression profiling in Escherichia coli K12: effects of oxygen availability and ArcA. J Biol Chem. 2005;280(15):15084–15096. doi:10.1074/jbc.M414030200.
   PubMed Web of Science ®Google Scholar
• Alvarez AF, Rodriguez C, Georgellis D. Ubiquinone and menaquinone electron carriers represent the yin and yang in the redox regulation of the ArcB sensor kinase. J Bacteriol. 2013;195(13):3054–3061. doi:10.1128/JB.00406-13.
   PubMed Web of Science ®Google Scholar
• Loui C, Chang AC, Lu S. Role of the ArcAB two-component system in the resistance of Escherichia coli to reactive oxygen stress. BMC Microbiol. 2009;9(1):183. doi:10.1186/1471-2180-9-183.
   PubMedGoogle Scholar
• van Beilen JW, Hellingwerf KJ, van Beilen JWA. All three endogenous quinone species of Escherichia coli are involved in controlling the activity of the aerobic/anaerobic response regulator ArcA. Front Microbiol. 2016;7:1339. doi:10.3389/fmicb.2016.01339.
   PubMed Web of Science ®Google Scholar
• Xie Y, Li J, Ding Y, Shao X, Sun Y, Xie F, Liu S, Tang S, Deng X. An atlas of bacterial two-component systems reveals function and plasticity in signal transduction. Cell Rep. 2022;41(3):111502. doi:10.1016/j.celrep.2022.111502.
   PubMed Web of Science ®Google Scholar
• Imelio JA, Trajtenberg F, Buschiazzo A. Allostery and protein plasticity: the keystones for bacterial signaling and regulation. Biophys Rev. 2021;13(6):943–953. doi:10.1007/s12551-021-00892-9.
   PubMedGoogle Scholar
• Ritchie JM, Rui H, Zhou X, Iida T, Kodoma T, Ito S, Davis BM, Bronson RT, Waldor MK, Valdivia RH. Inflammation and disintegration of intestinal villi in an experimental model for Vibrio parahaemolyticus-induced diarrhea. PLoS Pathog. 2012;8(3):e1002593. doi:10.1371/journal.ppat.1002593.
   PubMed Web of Science ®Google Scholar
• Gu D, Zhang Y, Wang Q, Zhou X. S-nitrosylation-mediated activation of a histidine kinase represses the type 3 secretion system and promotes virulence of an enteric pathogen. Nat Commun. 2020;11(1):5777. doi:10.1038/s41467-020-19506-1.
   PubMed Web of Science ®Google Scholar
• Lu R, Osei-Adjei G, Huang X, Zhang Y. Role and regulation of the orphan AphA protein of quorum sensing in pathogenic Vibrios. Future Microbiol. 2018;13(3):383–391. doi:10.2217/fmb-2017-0165.
   PubMed Web of Science ®Google Scholar
• Wang L, Ling Y, Jiang H, Qiu Y, Qiu J, Chen H, Yang R, Zhou D. AphA is required for biofilm formation, motility, and virulence in pandemic Vibrio parahaemolyticus. Int J Food Microbiol. 2013;160(3):245–251. doi:10.1016/j.ijfoodmicro.2012.11.004.
   PubMed Web of Science ®Google Scholar