Immortalization of primary marmoset skin fibroblasts by CRISPR-Cas9-mediated gene targeting

References

• Ahuja D, Saenz-Robles MT, Pipas JM. 2005. SV40 large T antigen targets multiple cellular pathways to elicit cellular transformation. Oncogene. 24(52):7729–7745.
   PubMed Web of Science ®Google Scholar
• Baugh EH, Ke H, Levine AJ, Bonneau RA, Chan CS. 2018. Why are there hotspot mutations in the TP53 gene in human cancers? Cell Death Differ. 25(1):154–160.
   PubMed Web of Science ®Google Scholar
• Blackburn EH. 2000. The end of the (DNA) line. Nat Struct Biol. 7(10):847–850.
   PubMedGoogle Scholar
• Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE. 1998. Extension of life-span by introduction of telomerase into normal human cells. Science. 279(5349):349–352.
   PubMed Web of Science ®Google Scholar
• Cho SW, Kim S, Kim JM, Kim JS. 2013. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol. 31(3):230–232.
   PubMed Web of Science ®Google Scholar
• Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, et al. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science. 339(6121):819–823.
   PubMed Web of Science ®Google Scholar
• Dumenco L, Oguey D, Wu J, Messier N, Fausto N. 1995. Introduction of a murine P53 mutation corresponding to human codon-249 into a murine hepatocyte cell-line results in growth advantage, but not in transformation. Hepatology. 22(4):1279–1288. English.
   PubMed Web of Science ®Google Scholar
• Gao Q, Hauser SH, Liu XL, Wazer DE, Madoc-Jones H, Band V. 1996. Mutant p53-induced immortalization of primary human mammary epithelial cells. Cancer Res. 56(13):3129–3133.
   PubMed Web of Science ®Google Scholar
• Guo Z, Jing R, Rao Q, Zhang L, Gao Y, Liu F, Wang X, Hui L, Yin H. 2018. Immortalized common marmoset (Callithrix jacchus) hepatic progenitor cells possess bipotentiality in vitro and in vivo. Cell Discov. 4:23.
   PubMed Web of Science ®Google Scholar
• Han NR, Baek S, Kim HY, Lee KY, Yun JI, Choi JH, Lee E, Park CK, Lee ST. 2020. Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice. Anim Cells Syst. 24(2):91–98.
   PubMed Web of Science ®Google Scholar
• Harvey DM, Levine AJ. 1991. P53 alteration is a common event in the spontaneous immortalization of primary BALB/c murine embryo fibroblasts. Genes Dev. 5(12B):2375–2385.
   PubMed Web of Science ®Google Scholar
• Harvey M, Sands AT, Weiss RS, Hegi ME, Wiseman RW, Pantazis P, Giovanella BC, Tainsky MA, Bradley A, Donehower LA. 1993. In vitro growth characteristics of embryo fibroblasts isolated from p53-deficient mice. Oncogene. 8(9):2457–2467.
   PubMed Web of Science ®Google Scholar
• Hayflick L. 1965. The limited in vitro lifetime of human diploid cell strains. Exp Cell Res. 37:614–636.
   PubMed Web of Science ®Google Scholar
• Huang P, Zhang L, Gao Y, He Z, Yao D, Wu Z, Cen J, Chen X, Liu C, Hu Y, et al. 2014. Direct reprogramming of human fibroblasts to functional and expandable hepatocytes. Cell Stem Cell. 14(3):370–384.
   PubMed Web of Science ®Google Scholar
• Kamijo T, Zindy F, Roussel MF, Quelle DE, Downing JR, Ashmun RA, Grosveld G, Sherr CJ. 1997. Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF. Cell. 91(5):649–659.
   PubMed Web of Science ®Google Scholar
• Kim D, Kim S, Kim S, Park J, Kim JS. 2016. Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex digenome-seq. Genome Res. 26(3):406–15.
   PubMed Web of Science ®Google Scholar
• Kim DY, Moon SH, Han JH, Kim MJ, Oh SJ, Bharti D, Lee SH, Park JK, Rho GJ, Jeon BG. 2020. Terminal differentiation into adipocyte and growth inhibition by PPARgamma activation in human A549 lung adenocarcinoma cells. Anim Cells Syst. 24(6):329–340.
   PubMed Web of Science ®Google Scholar
• Kishi N, Sato K, Sasaki E, Okano H. 2014. Common marmoset as a new model animal for neuroscience research and genome editing technology. Dev Growth Differ. 56(1):53–62.
   PubMed Web of Science ®Google Scholar
• Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science. 339(6121):823–826.
   PubMed Web of Science ®Google Scholar
• Malukiewicz J, Boere V, Fuzessy LF, Grativol AD, Silva IDE, Pereira LCM, Ruiz-Miranda CR, Valenca YM, Stone AC. 2015. Natural and anthropogenic hybridization in two species of eastern Brazilian marmosets (Callithrix jacchus and C. penicillata). PLoS One. 10(6):e0127268. English.
   PubMed Web of Science ®Google Scholar
• Oh SH, Lee HJ, Ahn MK, Jeon MY, Yoon JS, Jung YJ, Kim GN, Baek IJ, Kim I, Kim KM, et al. 2021. Multiplex gene targeting in the mouse embryo using a Cas9-Cpf1 hybrid guide RNA. Biochem Biophys Res Commun. 539:48–55.
   PubMed Web of Science ®Google Scholar
• Orimoto A, Shinohara H, Eitsuka T, Nakagawa K, Sasaki E, Kiyono T, Fukuda T. 2022. Immortalization of common marmoset-derived fibroblasts via expression of cell cycle regulators using the piggyBac transposon. Tissue Cell. 77:101848.
   PubMed Web of Science ®Google Scholar
• Petkov S, Kahland T, Shomroni O, Lingner T, Salinas G, Fuchs S, Debowski K, Behr R. 2018. Immortalization of common marmoset monkey fibroblasts by piggyBac transposition of hTERT. PLoS One. 13(9):e0204580.
   PubMed Web of Science ®Google Scholar
• Quelle DE, Zindy F, Ashmun RA, Sherr CJ. 1995. Alternative reading frames of the INK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest. Cell. 83(6):993–1000.
   PubMed Web of Science ®Google Scholar
• Seluanov A, Vaidya A, Gorbunova V. 2010. Establishing primary adult fibroblast cultures from rodents. J Vis Exp. 44:e2033.
   Google Scholar
• Serrano M, Lee H, Chin L, Cordon-Cardo C, Beach D, DePinho RA. 1996. Role of the INK4a locus in tumor suppression and cell mortality. Cell. 85(1):27–37.
   PubMed Web of Science ®Google Scholar
• Sharpless NE, Bardeesy N, Lee KH, Carrasco D, Castrillon DH, Aguirre AJ, Wu EA, Horner JW, DePinho RA. 2001. Loss of p16Ink4a with retention of p19Arf predisposes mice to tumorigenesis. Nature. 413(6851):86–91.
   PubMed Web of Science ®Google Scholar
• Sharpless NE, DePinho RA. 1999. The INK4A/ARF locus and its two gene products. Curr Opin Genet Dev. 9(1):22–30.
   PubMed Web of Science ®Google Scholar
• Shay JW, Zou Y, Hiyama E, Wright WE. 2001. Telomerase and cancer. Hum Mol Genet. 10(7):677–685.
   PubMed Web of Science ®Google Scholar
• Sung YH, Jin Y, Kang Y, Devkota S, Lee J, Roh JI, Lee HW. 2013. Ei24, a novel E2F target gene, affects p53-independent cell death upon ultraviolet C irradiation. J Biol Chem. 288(43):31261–31267.
   PubMed Web of Science ®Google Scholar
• Tsai SQ, Zheng Z, Nguyen NT, Liebers M, Topkar VV, Thapar V, Wyvekens N, Khayter C, Iafrate AJ, Le LP, et al. 2015. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat Biotechnol. 33(2):187–197.
   PubMed Web of Science ®Google Scholar
• Wolfel T, Hauer M, Schneider J, Serrano M, Wolfel C, Klehmann-Hieb E, De Plaen E, Hankeln T, Meyer zum Buschenfelde KH, Beach D. 1995. A p16INK4a-insensitive CDK4 mutant targeted by cytolytic T lymphocytes in a human melanoma. Science. 269(5228):1281–1284.
   PubMed Web of Science ®Google Scholar
• Zuo L, Weger J, Yang Q, Goldstein AM, Tucker MA, Walker GJ, Hayward N, Dracopoli NC. 1996. Germline mutations in the p16INK4a binding domain of CDK4 in familial melanoma. Nat Genet. 12(1):97–99.
   PubMed Web of Science ®Google Scholar