2,456
Views
7
CrossRef citations to date
0
Altmetric
Research Article

A Dual Zinc plus Arginine formulation attenuates the pathogenic properties of Porphyromonas gingivalis and protects gingival keratinocyte barrier function in an in vitro model

, , , &
Article: 1798044 | Received 07 Apr 2020, Accepted 15 Jul 2020, Published online: 04 Aug 2020

References

  • Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000. 1994;5:78–12.
  • Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010;8:481–490.
  • Bostanci N, Belibasakis GN. Porphyromonas gingivalis: an invasive and evasive opportunistic oral pathogen. FEMS Microbiol Lett. 2012;333:1–9.
  • How KY, Song KP, Chan KG. Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Front Microbiol. 2016;7:53.
  • Hajishengallis G, Lamont RJ. Beyond the red complex and into more complexity: the polymicrobial synergy and dysbiosis (PSD) model of periodontal disease etiology. Mol Oral Microbiol. 2012;27:409–419.
  • Guo Y, Nguyen KA, Potempa J. Dichotomy of gingipains action as virulence factors: from cleaving substrates with the precision of a surgeon’s knife to a meat chopper-like brutal degradation of proteins. Periodontol 2000. 2010;54:15–44.
  • Mysak J, Podzimek S, Sommerova P, et al. Porphyromonas gingivalis: major periodontopathic pathogen overview. J Immunol Res. 2014:476068.
  • Dale BA. Periodontal epithelium: a newly recognized role in health and disease. Periodontol 2000. 2002;30:70–78.
  • Groeger SE, Meyle J. Epithelial barrier and oral bacterial infection. Periodontol 2000. 2015;69:46–67.
  • Forster C. Tight junctions and the modulation of barrier function in disease. Histochem Cell Biol. 2008;130:55–70.
  • Groeger S, Doman E, Chakraborty T, et al. Effects of Porphyromonas gingivalis infection on human gingival epithelial barrier function in vitro. Eur J Oral Sci. 2010;118:582–589.
  • Hajishengallis G. Periodontitis: from microbial immune subversion to systemic inflammation. Nat Rev Immunol. 2015;15:30–44.
  • Han YW, Wang X. Mobile microbiome: oral bacteria in extra-oral infections and inflammation. J Dent Res. 2013;92:485–491.
  • Delgado E, Garcia-Godoy F, Montero-Aguilar M, et al. A clinical investigation of a Dual Zinc plus Arginine dentifrice in reducing established dental plaque and gingivitis over a six-month period of product use. J Clin Dent. 2018;29:A33–A40.
  • Prasad KV, Therathil SG, Agnihotri A, et al. The effects of two new Dual Zinc plus Arginine dentifrices in reducing oral bacteria in multiple locations in the mouth: 12-hour whole mouth antibacterial protection for whole mouth health. J Clin Dent. 2018;29:A25–A32.
  • Groeger S, Michel J, Meyle J. Establishment and characterization of immortalized human gingival keratinocyte cell lines. J Periodontal Res. 2008;43:604–614.
  • Gumbiner B, Simons K. A functional assay for proteins involved in establishing an epithelial occludin barrier: identification of a uvomorulin-like polypeptide. J Cell Biol. 1986;102:457–468.
  • Ben Lagha A, Groeger S, Meyle J, et al. Green tea polyphenols enhance gingival keratinocyte integrity and protect against invasion by Porphyromonas gingivalis. Pathog Dis. 2018;76:FTY030.
  • Marquis A, Genovese S, Epifano F, et al. The plant coumarins auraptene and lacinartin as potential multifunctional therapeutic agents for treating periodontal disease. BMC Complement Altern Med. 2012;12:80.
  • Bodet C, Chandad F, Grenier D. Hemoglobin and LPS act in synergy to amplify the inflammatory response. J Dent Res. 2007;86:878–882.
  • Houle MA, Grenier D, Plamondon P, et al. The collagenase activity of Porphyromonas gingivalis is due to Arg-gingipain. FEMS Microbiol Lett. 2003;221:181–185.
  • Imamura T. The role of gingipains in the pathogenesis of periodontal disease. J Periodontol. 2003;74:111–118.
  • Nishikata M, Yoshimura F, Nodasaka Y. Possibility of Bacteroides gingivalis hemagglutinin possessing protease activity revealed by inhibition studies. Microbiol Immunol. 1989;33:75–80.
  • Krauser JA, Potempa J, Travis J, et al. Inhibition of arginine gingipains (RgpB and HRgpA) with benzamidine inhibitors: zinc increases inhibitory potency. Biol Chem. 2002;383:1193–1198.
  • Manus LM, Daep CA, Begum-Gafur R, et al. Enhanced in vitro zinc bioavailability through rational design of a Dual Zinc plus Arginine dentifrice. J Clin Dent. 2018;29:A10–A19.
  • Meyle J, Gultig K, Rascher G, et al. Transepithelial electrical resistance and tight junctions of human gingival keratinocytes. J Periodontal Res. 1999;34:214–222.
  • Ben Lagha A, Grenier D. Black tea theaflavins attenuate Porphyromonas gingivalis virulence properties, modulate gingival keratinocyte tight junction integrity and exert anti-inflammatory activity. J Periodontal Res. 2017;52:458–470.
  • Ben Lagha A, LeBel G, Grenier D. Dual action of highbush blueberry proanthocyanidins on Aggregatibacter actinomycetemcomitans and the host inflammatory response. BMC Complement Altern Med. 2018;18(1):10.
  • Rybakovsky E, Valenzano MC, Deis R, et al. Improvement of humanoralepithelialbarrier function and of tight junctions by micronutrients. J Agric Food Chem. 2017;65:10950–10958.
  • Dale BA, Fredericks LP. Antimicrobial peptides in the oral environment: expression and function in health and disease. Curr Issues Mol Biol. 2011;7:119–134.
  • Lombardo Bedran TB, Feghali K, Zhao L, et al. Green tea extract and its major constituent, epigallocatechin-3-gallate, induce epithelial beta-defensin secretion and prevent beta-defensin degradation by Porphyromonas gingivalis. J Periodontal Res. 2013;49:615–623.
  • Andrian E, Grenier D, Rouabhia M. Porphyromonas gingivalis-epithelial cell interactions in periodontitis. J Dent Res. 2006;85:392–403.
  • Sakanaka A, Takeuchi H, Kuboniwa M, et al. Dual lifestyle of Porphyromonas gingivalis in biofilm and gingival cells. Microb Pathog. 2016;94:42–47.
  • Katz J, Sambandam V, Wu JH, et al. Characterization of Porphyromonas gingivalis-induced degradation of epithelial cell junctional complexes. Infect Immun. 2000;68:1441–1449.
  • Sarkar P, Saha T, Sheikh IA, et al. Zinc ameliorates intestinal dysfunctions in shigellosis by reinstating claudin−2 and −4 on the membranes. Am J Physiol Gastrointest Liver Physiol. 2019;316:G229–G246.
  • Lamont RJ, Chan A, Belton CM, et al. Porphyromonas gingivalis invasion of gingival epithelial cells. Infect Immun. 1995;63:3878–3885.
  • Nakagawa I, Inaba H, Yamamura T, et al. Invasion of epithelial cells and proteolysis of cellular focal adhesion components by distinct types of Porphyromonas gingivalis fimbriae. Infect Immun. 2006;74:3773–3782.