8,901
Views
4
CrossRef citations to date
0
Altmetric
Research Article

A protocol for primary isolation and culture of adipose-derived stem cells and their phenotypic profile

, ORCID Icon, & ORCID Icon
Pages 42-50 | Received 18 Oct 2019, Accepted 25 Mar 2020, Published online: 19 May 2020

References

  • McIntosh K, Zvonic S, Garrett S, et al. The immunogenicity of human adipose-derived cells: temporal changes in vitro. Stem Cells (Dayton, Ohio) 2006 May;24(5):1246–1253.
  • Ryan JM, Barry FP, Murphy JM, et al. Mesenchymal stem cells avoid allogeneic rejection. J Inflammation. 2005 July;26;2(1):8.
  • Kern S, Eichler H, Stoeve J, et al. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells (Dayton, OH). 2006 May;24(5):1294–1301.
  • Gentile P, Orlandi A, Scioli MG, et al. Concise review: adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical implications for tissue engineering therapies in regenerative surgery. Stem cells Transl Med. 2012 Mar;1(3):230–236.
  • Fraser JK, Zhu M, Wulur I, et al. Adipose-derived stem cells. Methods Mol Biol (Clifton, NJ).2008;449:59–67.
  • Zimmerlin L, Donnenberg VS, Pfeifer ME, et al. Stromal vascular progenitors in adult human adipose tissue. Cytometry. 2010 Jan;77(1):22–30.
  • Dominici M, Le Blanc K, Mueller I. et al. Minimal criteria for defining multipotent mesenchymal stromal cells. Cytotherapy. 2006;8(4):315–317.
  • Bourin P, Bunnell BA, Casteilla L, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013 Jun;15(6):641–648.
  • Scuderi N, Tenna S, Spalvieri C, et al. Power-assisted lipoplasty versus traditional suction-assisted lipoplasty: comparative evaluation and analysis of output. Aesthetic Plastic Surgery. 2005 Jan-Feb;29(1):49–52.
  • Raposio E, Simonacci F, Perrotta RE. Adipose-derived stem cells: comparison between two methods of isolation for clinical applications. Ann Med Surgery. 2017 Aug; 20:87–91.
  • Raposio E, Bertozzi N. Isolation of ready-to-use Adipose-Derived Stem Cell (ASC) pellet for clinical applications and a comparative overview of alternate methods for ASC isolation. Curr Protoc Stem Cell Biol. 2017 May 16;41(1):1F 17 1–1F 17 12.
  • Chung MT, Zimmermann AS, Paik KJ, et al. Isolation of human adipose-derived stromal cells using laser-assisted liposuction and their therapeutic potential in regenerative medicine. Stem Cells Transl Med. 2013 Oct;2(10):808–817.
  • Duscher D, Maan ZN, Luan A, et al. Ultrasound-assisted liposuction provides a source for functional adipose-derived stromal cells. Cytotherapy. 2017 Dec;19(12):1491–1500.
  • Baer PC, Geiger H. Adipose-derived mesenchymal stromal/stem cells: tissue localization, characterization, and heterogeneity. Stem Cells Int. 2012;2012:812693.
  • Cervelli V, Scioli MG, Gentile P, et al. Platelet-rich plasma greatly potentiates insulin-induced adipogenic differentiation of human adipose-derived stem cells through a serine/threonine kinase Akt-dependent mechanism and promotes clinical fat graft maintenance. Stem Cells Transl Med. 2012 Mar;1(3):206–220.
  • Zeng G, Lai K, Li J, et al. A rapid and efficient method for primary culture of human adipose-derived stem cells. Organogenesis. 2013 Oct 1;9(4):287–295.
  • Doucet C, Ernou I, Zhang Y, et al. Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications. J Cell Physiol. 2005 Nov;205(2):228–236.
  • Priya N, Sarcar S, Majumdar AS, et al. Explant culture: a simple, reproducible, efficient and economic technique for isolation of mesenchymal stromal cells from human adipose tissue and lipoaspirate. J Tissue Eng Regen Med. 2014 Sep;8(9):706–716.
  • Jing W, Xiao J, Xiong Z, et al. Explant culture: an efficient method to isolate adipose-derived stromal cells for tissue engineering. Artif Organs. 2011 Feb;35(2):105–112.
  • Frye CA, Wu X, Patrick CW. Microvascular endothelial cells sustain preadipocyte viability under hypoxic conditions. In Vitro Cell Dev Biol Anim. 2005 May-Jun;41(5):160–164.
  • Hutley LJ, Herington AC, Shurety W, et al. Human adipose tissue endothelial cells promote preadipocyte proliferation. Am J Physiol-Endocrinol Metab. 2001 Nov;281(5):E1037–E1044.
  • Traktuev DO, Merfeld-Clauss S, Li J, et al. A population of multipotent CD34-positive adipose stromal cells share pericyte and mesenchymal surface markers, reside in a periendothelial location, and stabilize endothelial networks. Circ Res. 2008 Jan 4;102(1):77–85.
  • Bochev I, Elmadjian G, Kyurkchiev D, et al. Mesenchymal stem cells from human bone marrow or adipose tissue differently modulate mitogen-stimulated B-cell immunoglobulin production in vitro. Cell Biol Int. 2008 Apr;32(4):384–393.
  • Duscher D, Luan A, Rennert RC, et al. Suction assisted liposuction does not impair the regenerative potential of adipose derived stem cells. J Transl Med. 2016 May 6;14(1):126.
  • Lin C-S, Xin Z-C, Deng C-H, et al. Defining adipose tissue-derived stem cells in tissue and in culture. Histol Histopathol. 2010 Jun;25(6):807–815.
  • Aust L, Devlin B, Foster SJ, et al. Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy. 2004;6(1):7–14.
  • Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001 Apr;7(2):211–228.
  • Jeffery E, Wing A, Holtrup B, et al. The adipose tissue microenvironment regulates depot-specific adipogenesis in obesity. Cell Metab. 2016 Jul 12;24(1):142–150.
  • Oedayrajsingh-Varma MJ, van Ham SM, Knippenberg M, et al. Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy. 2006;8(2):166–177.
  • Tchoukalova YD, Koutsari C, Votruba SB, et al. Sex- and depot-dependent differences in adipogenesis in normal-weight humans. Obesity. 2010 Oct;18(10):1875–1880.
  • Mischen BT, Follmar KE, Moyer KE, et al. Metabolic and functional characterization of human adipose-derived stem cells in tissue engineering. Plast Reconstr Surg. 2008 Sep;122(3):725–738.
  • Astori G, Vignati F, Bardelli S, et al. “In vitro“ and multicolor phenotypic characterization of cell subpopulations identified in fresh human adipose tissue stromal vascular fraction and in the derived mesenchymal stem cells. J Transl Med. 2007 Oct;5(1):55.
  • Maumus M, Peyrafitte J-A, D’Angelo R, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obesity. 2011 Sep;35(9):1141–1153.
  • Kim DS, Lee MW, Yoo KH, et al. Gene expression profiles of human adipose tissue-derived mesenchymal stem cells are modified by cell culture density. PloS One. 2014;9(1):e83363.