A new human adipocyte model with PTEN haploinsufficiency

References

• Bray GA, Kim KK, Wilding JPH. Obesity: a chronic relapsing progressive disease process. A position statement of the World Obesity Federation. Obes Rev. 2017;18:290–301.
   Web of Science ®Google Scholar
• White U, Tchoukalova Y. Sex dimorphism and depot differences in adipose tissue function. Biochim Biophys Acta. 2014;1842:377–392.
   PubMed Web of Science ®Google Scholar
• Wabitsch M, Brüderlein S, Melzner I, et al. LiSa-2, a novel human liposarcoma cell line with a high capacity for terminal adipose differentiation. Int J Cancer. 2000;88:889–894.
   PubMed Web of Science ®Google Scholar
• Rodriguez AM, Elabd C, Delteil F, et al. Adipocyte differentiation of multipotent cells established from human adipose tissue. Biochem Biophys Res Commun. 2004;315:255–263.
   PubMed Web of Science ®Google Scholar
• Wabitsch M, Brenner RE, Melzner I, et al. Characterization of a human preadipocyte cell strain with high capacity for adipose differentiation. Int J Obes Relat Metab Disord. 2001;25:8–15.
   PubMed Web of Science ®Google Scholar
• Schmid GL, Kässner F, Uhlig HH, et al. Sirolimus treatment of severe PTEN hamartoma tumor syndrome: case report and in vitro studies. Pediatr Res. 2014;75:527–534.
   PubMed Web of Science ®Google Scholar
• Ortega-Molina A, Efeyan A, Lopez-Guadamillas E, et al. Pten positively regulates brown adipose function, energy expenditure, and longevity. Cell Metab. 2012;15:382–394.
   PubMed Web of Science ®Google Scholar
• Manna P, Jain S. Hydrogen sulfide and L-cysteine increase PIP3 and glucose utilization by inhibiting PTEN protein and activating PI3K/AKT/PKCζ/λ in 3T3l1 adipocytes. J Biol Chem. 2011;286:39848–39859.
   PubMed Web of Science ®Google Scholar
• Lee SK, Lee JO, Kim JH, et al. Metformin sensitizes insulin signaling through AMPK-mediated PTEN down-regulation in preadipocyte 3T3-L1 cells. J Cell Biochem. 2011;112:1259–1267.
   PubMed Web of Science ®Google Scholar
• Pal A, Barber TM, Van de Bunt M, et al. PTEN mutations as a cause of constitutive insulin sensitivity and obesity. N Engl J Med. 2012;367:1002–1011.
   PubMed Web of Science ®Google Scholar
• Schmitt FC, Bacchi CE. S‐100 protein: is it useful as a tumour marker in diagnostic immunocytochemistry? Histopathology. 1989;15:281–288.
   PubMed Web of Science ®Google Scholar
• Klingelhutz AJ, Gourronc FA, Chaly A, et al. Scaffold-free generation of uniform adipose spheroids for metabolism research and drug discovery. Sci Rep. 2018;8:523.
   PubMed Web of Science ®Google Scholar
• Kirstein AS, Augustin A, Penke M, et al. The novel Phosphatidylinositol-3-Kinase (PI3K) inhibitor alpelisib effectively inhibits growth of PTEN-haploinsufficient lipoma cells. Cancers (Basel). 2019;11:1586.
   PubMed Web of Science ®Google Scholar
• Rueden CT, Schindelin J, Hiner MC, et al. ImageJ2: imageJ for the next generation of scientific image data. BMC Bioinformatics. 2017;18:529.
   PubMed Web of Science ®Google Scholar
• Revollo JRJR, Körner A, Mills KFKF, et al. Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme. Cell Metab. 2007;6:363–375.
   PubMed Web of Science ®Google Scholar
• Tannert A, Voigt P, Burgold S, et al. Signal amplification between Gbetagamma release and PI3Kgamma-mediated PI(3,4,5)P3 formation monitored by a fluorescent Gbetagamma biosensor protein and repetitive two component TIR/FRAP analysis. Biochemistry. 2008;47:11239–11250.
   PubMed Web of Science ®Google Scholar
• Wilhelm F, Kässner F, Schmid G, et al. Phosphatidylinositol 3-kinase (PI3K) signalling regulates insulin-like-growth factor binding protein-2 (IGFBP-2) production in human adipocytes. Growth Horm IGF Res. 2015;25:115–120.
   PubMed Web of Science ®Google Scholar
• Ngeow J, Eng C. PTEN hamartoma tumor syndrome: clinical risk assessment and management protocol. Methods. 2014. DOI:10.1016/j.ymeth.2014.10.011
   PubMed Web of Science ®Google Scholar
• Marsh DJ, Kum JB, Lunetta KL, et al. PTEN mutation spectrum and genotype-phenotype correlations in Bannayan-Riley-Ruvalcaba syndrome suggest a single entity with Cowden syndrome. Hum Mol Genet. 1999;8:1461–1472. Available from: http://www.ncbi.nlm.nih.gov/pubmed/10400993
   PubMed Web of Science ®Google Scholar
• Chalhoub N, Baker SJ. PTEN and the PI3-kinase pathway in cancer. Annu Rev Pathol. 2009;4:127–150.
   PubMed Web of Science ®Google Scholar
• Suga H, Eto H, Inoue K, et al. Cellular and molecular features of lipoma tissue: comparison with normal adipose tissue. Br J Dermatol. 2009;161:819–825.
   PubMed Web of Science ®Google Scholar
• Kim M, Rhee JK, Choi H, et al. Passage-dependent accumulation of somatic mutations in mesenchymal stromal cells during in vitro culture revealed by whole genome sequencing. Sci Rep. 2017;7. DOI:10.1038/s41598-017-15155-5.
   Web of Science ®Google Scholar
• Fischer-Posovszky P, Newell FS, Wabitsch M, et al. NFS. Human SGBS cells - a unique tool for studies of human fat cell biology. Obes Facts. 2008;1:184–189.
   PubMed Web of Science ®Google Scholar
• Yeo CR, Agrawal M, Hoon S, et al. SGBS cells as a model of human adipocyte browning: A comprehensive comparative study with primary human white subcutaneous adipocytes. Sci Rep. 2017;7:4031.
   PubMed Web of Science ®Google Scholar
• Allott EH, Oliver E, Lysaght J, et al. The SGBS cell strain as a model for the in vitro study of obesity and cancer. Clin Transl Oncol. 2012;14:774–782.
   PubMed Web of Science ®Google Scholar
• Galhardo M, Sinkkonen L, Berninger P, et al. Transcriptomics profiling of human SGBS adipogenesis. Genom Data. 2014;2:246–248.
   PubMedGoogle Scholar
• Keuper M, Dzyakanchuk A, Amrein KE, et al. THP-1 macrophages and SGBS adipocytes - a new human in vitro model system of inflamed adipose tissue. Front Endocrinol (Lausanne). 2011;2:89.
   PubMedGoogle Scholar
• McInnes KJ, Brown KA, Knower KC, et al. Characterisation of aromatase expression in the human adipocyte cell line SGBS. Breast Cancer Res Treat. 2008;112:429–435.
   PubMed Web of Science ®Google Scholar
• Díaz-Delfín J, Domingo P, Wabitsch M, et al. HIV-1 Tat protein impairs adipogenesis and induces the expression and secretion of proinflammatory cytokines in human SGBS adipocytes. Antivir Ther. 2012;17:529–540.
   PubMed Web of Science ®Google Scholar
• Leiherer A, Geiger K, Muendlein A, et al. Hypoxia induces a HIF-1α dependent signaling cascade to make a complex metabolic switch in SGBS-adipocytes. Mol Cell Endocrinol. 2014;383:21–31.
   PubMed Web of Science ®Google Scholar
• Kraus M, Greither T, Wenzel C, et al. Inhibition of adipogenic differentiation of human SGBS preadipocytes by androgen-regulated microRNA miR-375. Mol Cell Endocrinol. 2015;414:177–185.
   PubMed Web of Science ®Google Scholar
• Schaedlich K, Gebauer S, Hunger L, et al. DEHP deregulates adipokine levels and impairs fatty acid storage in human SGBS-adipocytes. Sci Rep. 2018;8. DOI:10.1038/s41598-018-21800-4.
   PubMed Web of Science ®Google Scholar
• Li S, Bouzar C, Cottet-Rousselle C, et al. Resveratrol inhibits lipogenesis of 3T3-L1 and SGBS cells by inhibition of insulin signaling and mitochondrial mass increase. Biochim Biophys Acta - Bioenergy. 2016;1857:643–652.
   PubMed Web of Science ®Google Scholar
• Iwata T, Kuribayashi K, Nakasono M, et al. The AMPK/mTOR pathway is involved in D-dopachrome tautomerase gene transcription in adipocytes differentiated from SGBS cells, a human preadipocyte cell line. Cytokine. 2017;96:195–202.
   PubMed Web of Science ®Google Scholar
• Kehrer C, Hoischen A, Menkhaus R, et al. Whole exome sequencing and array-based molecular karyotyping as aids to prenatal diagnosis in fetuses with suspected Simpson-Golabi-Behmel syndrome. Prenat Diagn. 2016;36:961–965.
   PubMed Web of Science ®Google Scholar
• Qiu T, Tian Y, Gao Y, et al. PTEN loss regulates alveolar epithelial cell senescence in pulmonary fibrosis depending on Akt activation. Aging (Albany NY). 2019;11:7492–7509.
   PubMedGoogle Scholar
• Banasavadi-Siddegowda YK, Russell L, Frair E, et al. PRMT5-PTEN molecular pathway regulates senescence and self-renewal of primary glioblastoma neurosphere cells. Oncogene. 2017;36:263–274.
   PubMed Web of Science ®Google Scholar
• Kässner F, Sauer T, Penke M, et al. Simvastatin induces apoptosis in PTEN-haploinsufficient lipoma cells. Int J Mol Med. 2018;41. DOI:10.3892/ijmm.2018.3568.
   PubMed Web of Science ®Google Scholar
• Chen HH, Händel N, Ngeow J, et al. Immune dysregulation in patients with PTEN hamartoma tumor syndrome: analysis of FOXP3 regulatory T cells. J Allergy Clin Immunol. 2016;139:607–620.e15.
   PubMed Web of Science ®Google Scholar
• Goldstein BJ. Protein-tyrosine phosphatase 1B (PTP1B): a novel therapeutic target for type 2 diabetes mellitus, obesity and related states of insulin resistance. Current drug targets. Immune Endocr Metab Disord. 2001;1:265–275.
   PubMedGoogle Scholar
• De Lorenzo C, Greco A, Fiorentino TV, et al. Variants of insulin-signaling inhibitor genes in type 2 diabetes and related metabolic abnormalities. Int J Genomics. 2013;2013:1–13.
   PubMed Web of Science ®Google Scholar
• Heindl M, Händel N, Ngeow J, et al. Autoimmunity, intestinal lymphoid hyperplasia, and defects in mucosal B-cell homeostasis in patients with PTEN hamartoma tumor syndrome. Gastroenterology. 2012;142:1093–1096.e6.
   PubMed Web of Science ®Google Scholar