1,638
Views
0
CrossRef citations to date
0
Altmetric
Research Article

Novel semi-automated algorithm for high-throughput quantification of adipocyte size in breast adipose tissue, with applications for breast cancer microenvironment

, ORCID Icon, ORCID Icon, ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon show all
Pages 313-325 | Received 30 Dec 2019, Accepted 16 Jun 2020, Published online: 07 Jul 2020

References

  • Lund MJ, Butler EN, Bumpers HL, et al. High prevalence of triple-negative tumors in an urban cancer center. Cancer. 2008;113:608–615.
  • Brawley OW. Health disparities in breast cancer. Obstet Gynecol Clin North Am. 2013;40:513–523.
  • Ko NY, Fu JL, Caron S, et al. Cancer clinical trial enrollment within an urban safety net hospital. J Community Support Oncol. 2015;13:429–435.
  • Vimalananda VG, Rosenzweig JL, Cabral HJ, et al. Comparison of diabetes control among Haitians, African Americans, and non-Hispanic whites in an urban safety-net hospital. Diabetes Care. 2011;34:58–60.
  • Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA Cancer J Clin. 2019;69:7–34.
  • Kohler BA, Sherman RL, Howlader N, et al. Annual Report to the nation on the status of cancer, 1975–2011, featuring incidence of breast cancer subtypes by race/ethnicity, poverty, and state. J Natl Cancer Inst. 2015;107:djv048.
  • Hunt BR, Hurlbert MS. Black:white disparities in breast cancer mortality in the 50 largest cities in the United States, 2005-2014. Cancer Epidemiol. 2016;45:169–173.
  • Siegel RL, Jemal A, Wender RC, et al. An assessment of progress in cancer control. CA Cancer J Clin. 2018;68:329–339.
  • Canin B, Freund KM, Ganz PA, et al. Disparities in breast cancer care and research: report from a breast cancer research foundation sponsored workshop, 9-10 October 2014. NPJ Breast Cancer. 2015;1:15013.
  • Jaiswal K, Hull M, Furniss AL, et al. Delays in diagnosis and treatment of breast cancer: A safety-net population profile. J Natl Compr Canc Netw. 2018;16:1451–1457.
  • Wolf AM, Colditz GA. Current estimates of the economic cost of obesity in the United States. Obes Res. 1998;6:97–106.
  • Cossrow N, Falkner B. Race/ethnic issues in obesity and obesity-related comorbidities. J Clin Endocrinol Metab. 2004;89:2590–2594.
  • Dammann KW, Smith C. Factors affecting low-income women’s food choices and the perceived impact of dietary intake and socioeconomic status on their health and weight. J Nutr Educ Behav. 2009;41:242–253.
  • Simpson ER, Brown KA. Obesity and breast cancer: role of inflammation and aromatase. J Mol Endocrinol. 2013;51:T51–T59.
  • Nicholas DA, Andrieu G, Strissel KJ, et al. BET bromodomain proteins and epigenetic regulation of inflammation: implications for type 2 diabetes and breast cancer. Cell Mol Life Sci. 2017;74:231–243.
  • Denis GV, Sebastiani P, Andrieu G, et al. Relationships among obesity, type 2 diabetes, and plasma cytokines in African American women. Obesity (Silver Spring). 2017;25:1916–1920.
  • Palmer JR, Castro-Webb N, Bertrand K, et al. Type II diabetes and incidence of estrogen receptor negative breast cancer in African American women. Cancer Res. 2017;77:6462–6469.
  • Charlot M, Castro-Webb N, Bethea TN, et al. Diabetes and breast cancer mortality in black women. Cancer Causes Control. 2017;28:61–67.
  • West M, Blanchette C, Dressman H, et al. Predicting the clinical status of human breast cancer by using gene expression profiles. Proc Natl Acad Sci U S A. 2001;98:11462–11467.
  • Cronin M, Sangli C, Liu ML, et al. Analytical validation of the Oncotype DX genomic diagnostic test for recurrence prognosis and therapeutic response prediction in node-negative, estrogen receptor-positive breast cancer. Clin Chem. 2007;53:1084–1091.
  • Crowley MM, McCoy ME, Bak SM, et al. Challenges in the delivery of quality breast cancer care: initiation of adjuvant hormone therapy at an urban safety net hospital. J Oncol Pract. 2014;10:e107–112.
  • Khanna S, Kim KN, Qureshi MM, et al. Impact of patient demographics, tumor characteristics, and treatment type on treatment delay throughout breast cancer care at a diverse academic medical center. Int J Womens Health. 2017;9:887–896.
  • Albain K, Gray RJ, Sparano JA, et al. Abstract GS4-07: race, ethnicity and clinical outcomes in hormone receptor-positive, HER2-negative, node-negative breast cancer: results from the TAILORx trial. Cancer Res. 2019;79(4 Supplement):GS4–07.
  • Denis GV, Sebastiani P, Bertrand KA, et al. Inflammatory signatures distinguish metabolic health in African American women with obesity. PLoS One. 2018;13:e0196755.
  • Strissel KJ, Nicholas DA, Castagne-Charlotin M, et al. Barriers to obtaining sera and tissue specimens of African-American women for the advancement of cancer research. Clin Med Insights Womens Health. 2016;9(Suppl 1):57–61.
  • Di Girolamo M, Mendlinger S, Fertig JW. A simple method to determine fat cell size and number in four mammalian species. Am J Physiol. 1971;221:850–858.
  • Parlee SD, Lentz SI, Mori H, et al. Quantifying size and number of adipocytes in adipose tissue. Methods Enzymol. 2014;537:93–122.
  • Hirsch J, Gallian E. Methods for the determination of adipose cell size in man and animals. J Lipid Res. 1968;9:110–119.
  • Stern MP, Conrad F. An automated, direct method for measuring adipocyte cell size. Clin Chim Acta. 1975;65:29–37.
  • Osman OS, Selway JL, Kepczyńska MA, et al. A novel automated image analysis method for accurate adipocyte quantification. Adipocyte. 2013;2:160–164.
  • Zhi X, Wang J, Lu P, et al. AdipoCount: A new software for automatic adipocyte counting. Front Physiol. 2018;9:85.
  • Galarraga M, Campión J, Muñoz-Barrutia A, et al. Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections. J Lipid Res. 2012;53:2791–2796.. [published correction appears in J Lipid Res. 2014 Dec;55(12):2705].
  • Klöting N, Fasshauer M, Dietrich A, et al. Insulin-sensitive obesity. Am J Physiol Endocrinol Metab. 2010;299:E506–E515.
  • Klöting N, Blüher M. Adipocyte dysfunction, inflammation and metabolic syndrome. Rev Endocr Metab Disord. 2014;15:277–287.
  • Weisberg SP, McCann D, Desai M, et al. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest. 2003;112:1796–1808.
  • Apovian CM, Bigornia S, Mott M, et al. Adipose macrophage infiltration is associated with insulin resistance and vascular endothelial dysfunction in obese subjects. Arterioscler Thromb Vasc Biol. 2008;28:1654–1659.
  • Morris PG, Hudis CA, Giri D, et al. Inflammation and increased aromatase expression occur in the breast tissue of obese women with breast cancer. Cancer Prev Res (Phila). 2011;4:1021–1029.
  • Howe LR, Subbaramaiah K, Hudis CA, et al. Molecular pathways: adipose inflammation as a mediator of obesity-associated cancer. Clin Cancer Res. 2013;19:6074–6083.
  • Subbaramaiah K, Morris PG, Zhou XK, et al. Increased levels of COX-2 and prostaglandin E2 contribute to elevated aromatase expression in inflamed breast tissue of obese women. Cancer Discov. 2012;2:356–365.
  • Iyengar NM, Zhou XK, Gucalp A, et al. Systemic correlates of white adipose tissue inflammation in early-stage breast cancer. Clin Cancer Res. 2016;22:2283–2289.