Ex vivo observation of Pythium insidiosum-antigen treated neutrophils on three Pythium insidiosum strains isolated from vascular pythiosis patients

References

• Krajaejun T, Sathapatayavongs B, Pracharktam R, Nitiyanant P, Leelachaikul P, Wanachiwanawin W, Chaiprasert A, Assanasen P, Saipetch M, Mootsikapun P. et al. Clinical and epidemiological analyses of human pythiosis in Thailand. Clin Infect Dis. 2006;43(5):569–10. doi:10.1086/506353. Epub 2006/08/04. PubMed PMID: 16886148.
   PubMed Web of Science ®Google Scholar
• Chitasombat MN, Jongkhajornpong P, Lekhanont K, Krajaejun T. Recent update in diagnosis and treatment of human pythiosis. PeerJ. 2020;8:e8555. doi:10.7717/peerj.8555. Epub 2020/03/03. PubMed PMID: 32117626; PMCID: PMC7036273.
   PubMed Web of Science ®Google Scholar
• Sermsathanasawadi N, Praditsuktavorn B, Hongku K, Wongwanit C, Chinsakchai K, Ruangsetakit C, Hahtapornsawan S, Mutirangura P. Outcomes and factors influencing prognosis in patients with vascular pythiosis. J Vasc Surg. 2016;64(2):411–7. doi:10.1016/j.jvs.2015.12.024. Epub 2016/10/21. PubMed PMID: 26951999.
   PubMed Web of Science ®Google Scholar
• Supabandhu J, Fisher MC, Mendoza L, Vanittanakom N. Isolation and identification of the human pathogen Pythium insidiosum from environmental samples collected in Thai agricultural areas. Med Mycol. 2008;46(1):41–52. doi:10.1080/13693780701513840. Epub 2007/09/22. PubMed PMID: 17885956.
   PubMed Web of Science ®Google Scholar
• Chitasombat MN, Larbcharoensub N, Chindamporn A, Krajaejun T. Clinicopathological features and outcomes of pythiosis. Int J Infect Dis. 2018;71:33–41. doi:10.1016/j.ijid.2018.03.021. Epub 2018/04/14. PubMed PMID: 29653202.
   PubMed Web of Science ®Google Scholar
• Sudjaritruk T, Sirisanthana V. Successful treatment of a child with vascular pythiosis. BMC Infect Dis. 2011;11:33. doi:10.1186/1471-2334-11-33. Epub 2011/02/01. PubMed PMID: 21276255; PMCID: PMC3039579.
   PubMed Web of Science ®Google Scholar
• Reanpang T, Orrapin S, Orrapin S, Arworn S, Kattipatanapong T, Srisuwan T, Vanittanakom N, Lekawanvijit SP, Rerkasem K. Vascular pythiosis of the lower extremity in Northern Thailand: ten years’ experience. Int J Low Extrem Wounds. 2015;14(3):245–50. doi:10.1177/1534734615599652. Epub 2015/08/20. PubMed PMID: 26286930.
   PubMed Web of Science ®Google Scholar
• Naran K, Nundalall T, Chetty S, Barth S. Principles of immunotherapy: implications for treatment strategies in cancer and infectious diseases. Front Microbiol. 2018;9:3158. doi:10.3389/fmicb.2018.03158. Epub 2019/01/10. PubMed PMID: 30622524; PMCID: PMC6308495.
   PubMed Web of Science ®Google Scholar
• Yolanda H, Krajaejun T. History and perspective of immunotherapy for pythiosis. Vaccines (Basel). 2021;9(10):1080. doi:10.3390/vaccines9101080. Epub 2021/10/27. PubMed PMID: 34696188; PMCID: PMC8539095.
   PubMed Web of Science ®Google Scholar
• Wanachiwanawin W, Mendoza L, Visuthisakchai S, Mutsikapan P, Sathapatayavongs B, Chaiprasert A, Suwanagool P, Manuskiatti W, Ruangsetakit C, Ajello L. Efficacy of immunotherapy using antigens of Pythium insidiosum in the treatment of vascular pythiosis in humans. Vaccine. 2004;22(27–28):3613–21. doi:10.1016/j.vaccine.2004.03.031. Epub 2004/08/19. PubMed PMID: 15315840.
   PubMed Web of Science ®Google Scholar
• Mendoza L, Mandy W, Glass R. An improved Pythium insidiosum-vaccine formulation with enhanced immunotherapeutic properties in horses and dogs with pythiosis. Vaccine. 2003;21(21–22):2797–804. doi:10.1016/s0264-410x(03)00225-1. Epub 2003/06/12. PubMed PMID: 12798620.
   PubMed Web of Science ®Google Scholar
• Mendoza L, Newton JC. Immunology and immunotherapy of the infections caused by Pythium insidiosum. Med Mycol. 2005;43(6):477–86. doi:10.1080/13693780500279882. Epub 2005/12/03. PubMed PMID: 16320491.
   PubMed Web of Science ®Google Scholar
• Worasilchai N, Permpalung N, Chongsathidkiet P, Leelahavanichkul A, Mendoza AL, Palaga T, Reantragoon R, Finkelman M, Sutcharitchan P, Chindamporn A. Monitoring anti-Pythium insidiosum IgG Antibodies and (1-->3)-beta-d-Glucan in vascular pythiosis. J Clin Microbiol. 2018;56(8). doi:10.1128/JCM.00610-18. Epub 2018/06/01. PubMed PMID: 29848566; PMCID: PMC6062813.
   PubMed Web of Science ®Google Scholar
• Kobayashi SD, DeLeo FR. Role of neutrophils in innate immunity: a systems biology-level approach. Wiley Interdiscip Rev Syst Biol Med. 2009;1(3):309–33. doi:10.1002/wsbm.32. Epub 2010/09/14. PubMed PMID: 20836000; PMCID: PMC3501127.
   PubMed Web of Science ®Google Scholar
• Kumar V, Sharma A. Neutrophils: Cinderella of innate immune system. Int Immunopharmacol. 2010;10(11):1325–34. doi:10.1016/j.intimp.2010.08.012. Epub 2010/09/11. PubMed PMID: 20828640.
   PubMed Web of Science ®Google Scholar
• Wongprompitak P, Pleewan N, Tantibhedhyangkul W, Chaiprasert A, Prabhasawat P, Inthasin N, Ekpo P. Involvement of toll-like receptor 2 on human corneal epithelium during an infection of Pythium insidiosum. Asian Pac J Allergy Immunol. 2020;38(2):129–38. doi:10.12932/AP-110518-0311. Epub 2018/08/18. PubMed PMID: 30118247.
   PubMed Web of Science ®Google Scholar
• Ahirwar LK, Sharma S. Elevated levels of interleukins, leukocyte protein and cathelicidin antimicrobial peptide are strongly associated with early to mid-stage of Pythium insidiosum infection in rabbit corneas. Curr Eye Res. 2022;47(5):677–87. doi:10.1080/02713683.2021.2023192. Epub 2022/02/19. PubMed PMID: 35179411.
   PubMed Web of Science ®Google Scholar
• Cesta MC, Zippoli M, Marsiglia C, Gavioli EM, Mantelli F, Allegretti M, Balk RA. The role of interleukin-8 in lung inflammation and injury: implications for the management of COVID-19 and hyperinflammatory acute respiratory distress syndrome. Front Pharmacol. 2021;12:808797. doi:10.3389/fphar.2021.808797. Epub 2022/02/01. PubMed PMID: 35095519; PMCID: PMC8790527.
   PubMed Web of Science ®Google Scholar
• Sriwarom A, Chiewchengchol D, Saithong S, Worasilchai N, Chindamporn A, Krajaejun T. Neutrophil extracellular traps and phagocytosis in Pythium insidiosum. PLoS One. 2023;18(1):e0280565. doi:10.1371/journal.pone.0280565. Epub 2023/01/25. PubMed PMID: 36693041; PMCID: PMC9873155.
   PubMed Web of Science ®Google Scholar
• Torvorapanit P, Chuleerarux N, Plongla R, Worasilchai N, Manothummetha K, Thongkam A, Langsiri N, Diewsurin J, Kongsakpaisan P, Bansong R. et al. Clinical outcomes of radical surgery and antimicrobial agents in vascular pythiosis: a multicenter prospective study. J Fungi (Basel). 2021;7(2):114. doi:10.3390/jof7020114. Epub 2021/02/10. PubMed PMID: 33557064; PMCID: PMC7913857.
   PubMedGoogle Scholar
• Permpalung N, Worasilchai N, Chindamporn A. Human pythiosis: emergence of fungal-like organism. Mycopathologia. 2020;185(5):801–12. doi:10.1007/s11046-019-00412-0. Epub 2019/12/18. PubMed PMID: 31845178.
   PubMed Web of Science ®Google Scholar
• Degel J, Shokrani M. Validation of the efficacy of a practical method for neutrophils isolation from peripheral blood. Clin Lab Sci. 2010;23(2):94–8. doi:10.29074/ascls.23.2.94. Epub 2010/05/27. PubMed PMID: 20499533.
   PubMedGoogle Scholar
• Mendoza L, Prendas J. A method to obtain rapid zoosporogenesis of Pythium insidiosum. Mycopathologia. 1988;104(1):59–62. doi:10.1007/BF00437925. Epub 1988/10/01. PubMed PMID: 3216883.
   PubMed Web of Science ®Google Scholar
• Chaiprasert A, Samerpitak K, Wanachiwanawin W, Thasnakorn P. Induction of zoospore formation in Thai isolates of Pythium insidiosum. Mycoses. 1990;33(6):317–23. doi:10.1111/myc.1990.33.6.317. Epub 1990/06/01. PubMed PMID: 2259373.
   PubMed Web of Science ®Google Scholar
• Kumar P, Nagarajan A, Uchil PD. Analysis of cell viability by the MTT assay. Cold Spring Harb Protoc. 2018;2018(6). doi:10.1101/pdb.prot095505. Epub 2018/06/03. PubMed PMID: 29858338.
   Google Scholar
• Kamiloglu S, Sari G, Ozdal T, Capanoglu E. Guidelines for cell viability assays. Food Front. 2020;1(3):332–49. doi:10.1002/fft2.44.
   Google Scholar
• Stoimenou M, Tzoros G, Skendros P, Chrysanthopoulou A. Methods for the assessment of NET formation: from neutrophil biology to translational research. Int J Mol Sci. 2022;23(24). doi:10.3390/ijms232415823. Epub 2022/12/24. PubMed PMID: 36555464; PMCID: PMC9781911.
   PubMed Web of Science ®Google Scholar
• Sae-Khow K, Tachaboon S, Wright HL, Edwards SW, Srisawat N, Leelahavanichkul A, Chiewchengchol D. Defective neutrophil function in patients with sepsis is mostly restored by ex vivo ascorbate incubation. J Inflamm Res. 2020;13:263–74. doi:10.2147/JIR.S252433. Epub 2020/07/09. PubMed PMID: 32636666; PMCID: PMC7326689.
   PubMed Web of Science ®Google Scholar
• Gaastra W, Lipman LJ, De Cock AW, Exel TK, Pegge RB, Scheurwater J, Vilela R, Mendoza L. Pythium insidiosum: an overview. Vet Microbiol. 2010;146(1–2):1–16. doi:10.1016/j.vetmic.2010.07.019. Epub 2010/08/31. PubMed PMID: 20800978.
   PubMed Web of Science ®Google Scholar
• Vanholder R, Glorieux G. 2 - Uremic toxicity. In: Nissenson A; Fine R; Mehrotra R Zaritsky J. editors. Handbook of dialysis therapy. 6th ed. New Delhi: Elsevier; 2023. p. 16–44.
   Google Scholar
• Zeilhofer HU, Schorr W. Role of interleukin-8 in neutrophil signaling. Curr Opin Hematol. 2000;7(3):178–82. doi:10.1097/00062752-200005000-00009. Epub 2000/04/29. PubMed PMID: 10786656.
   PubMed Web of Science ®Google Scholar
• Ohta K, Ishida Y, Fukui A, Nishi H, Naruse T, Takechi M, Kamata N. Itraconazole inhibits TNF-alpha-induced CXCL10 expression in oral fibroblasts. Oral Dis. 2015;21(1):106–12. doi:10.1111/odi.12226. Epub 2014/02/06. PubMed PMID: 24495175.
   PubMed Web of Science ®Google Scholar
• Mizuno K, Fukami T, Toyoda Y, Nakajima M, Yokoi T. Terbinafine stimulates the pro-inflammatory responses in human monocytic THP-1 cells through an ERK signaling pathway. Life Sci. 2010;87(17–18):537–44. doi:10.1016/j.lfs.2010.08.010. Epub 2010/09/08. PubMed PMID: 20816994.
   PubMed Web of Science ®Google Scholar
• Skelly AC, Dettori JR, Brodt ED. Assessing bias: the importance of considering confounding. Evid Based Spine Care J. 2012;3(1):9–12. doi:10.1055/s-0031-1298595. Epub 2012/12/14. PubMed PMID: 23236300; PMCID: PMC3503514.
   PubMedGoogle Scholar
• Duffy SA, Teknos T, Taylor JM, Fowler KE, Islam M, Wolf GT, McLean S, Ghanem TA, Terrell JE. Health behaviors predict higher interleukin-6 levels among patients newly diagnosed with head and neck squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 2013;22(3):374–81. doi:10.1158/1055-9965.EPI-12-0987. Epub 2013/01/10. PubMed PMID: 23300019; PMCID: PMC3596441.
   PubMed Web of Science ®Google Scholar
• Nathan C. Neutrophils and immunity: challenges and opportunities. Nat Rev Immunol. 2006;6(3):173–82. doi:10.1038/nri1785. Epub 2006/02/25. PubMed PMID: 16498448.
   PubMed Web of Science ®Google Scholar
• Blanter M, Gouwy M, Struyf S. Studying neutrophil function in vitro: cell models and environmental factors. J Inflamm Res. 2021;14:141–62. doi:10.2147/JIR.S284941. Epub 2021/01/29. PubMed PMID: 33505167; PMCID: PMC7829132.
   PubMed Web of Science ®Google Scholar
• Stoddart MJ. Cell viability assays: introduction. Methods Mol Biol. 2011;740:1–6. doi:10.1007/978-1-61779-108-6_1. Epub 2011/04/07. PubMed PMID: 21468961.
   PubMedGoogle Scholar
• Stepanenko AA, Dmitrenko VV. Pitfalls of the MTT assay: direct and off-target effects of inhibitors can result in over/underestimation of cell viability. Gene. 2015;574(2):193–203. doi:10.1016/j.gene.2015.08.009. Epub 2015/08/12. PubMed PMID: 26260013.
   PubMed Web of Science ®Google Scholar
• Zanette RA, Ferreiro L, Alves SH, Jesus FP, Lautert C, Spanamberg A, Santurio JM. Enzymatic variability among Brazilian Pythium insidiosum isolates. Rev Iberoam Micol. 2013;30(4):264–6. doi:10.1016/j.riam.2012.12.005. Epub 2013/01/16. PubMed PMID: 23318164.
   PubMed Web of Science ®Google Scholar
• Krajaejun T, Rujirawat T, Kanpanleuk T, Santanirand P, Lohnoo T, Yingyong W, Kumsang Y, Sae-Chew P, Kittichotirat W, Patumcharoenpol P. Biochemical and genetic analyses of the oomycete Pythium insidiosum provide new insights into clinical identification and urease-based evolution of metabolism-related traits. PeerJ. 2018;6:e4821. doi:10.7717/peerj.4821. Epub 2018/06/12. PubMed PMID: 29888122; PMCID: PMC5993020.
   PubMed Web of Science ®Google Scholar
• Mayadas TN, Cullere X, Lowell CA. The multifaceted functions of neutrophils. Annu Rev Pathol. 2014;9:181–218. doi:10.1146/annurev-pathol-020712-164023. Epub 2013/09/21. PubMed PMID: 24050624; PMCID: PMC4277181.
   PubMed Web of Science ®Google Scholar
• Rada B. Neutrophil extracellular traps. Methods Mol Biol. 2019;1982:517–28. doi:10.1007/978-1-4939-9424-3_31. Epub 2019/06/07. PubMed PMID: 31172493; PMCID: PMC6874304.
   PubMedGoogle Scholar
• Chaiprasert A, Krajaejun T. 12. Pythiosis. In: Jones E; Kevin D Ka-Lai P. editors. Freshwater fungi. Berlin, Boston: De Gruyter; 2014. p. 263–78.
   Google Scholar
• Branzk N, Lubojemska A, Hardison SE, Wang Q, Gutierrez MG, Brown GD, Papayannopoulos V. Neutrophils sense microbe size and selectively release neutrophil extracellular traps in response to large pathogens. Nat Immunol. 2014;15(11):1017–25. doi:10.1038/ni.2987. Epub 2014/09/15. PubMed PMID: 25217981; PMCID: PMC4236687.
   PubMed Web of Science ®Google Scholar
• Thalin C, Hisada Y, Lundstrom S, Mackman N, Wallen H. Neutrophil extracellular traps: villains and targets in arterial, venous, and cancer-associated thrombosis. Arterioscler Thromb Vasc Biol. 2019;39(9):1724–38. doi:10.1161/ATVBAHA.119.312463. Epub 2019/07/19. PubMed PMID: 31315434; PMCID: PMC6703916.
   PubMed Web of Science ®Google Scholar
• Fuchs TA, Abed U, Goosmann C, Hurwitz R, Schulze I, Wahn V, Weinrauch Y, Brinkmann V, Zychlinsky A. Novel cell death program leads to neutrophil extracellular traps. J Cell Biol. 2007;176(2):231–41. doi:10.1083/jcb.200606027. Epub 2007/01/11. PubMed PMID: 17210947; PMCID: PMC2063942.
   PubMed Web of Science ®Google Scholar
• Langseth MS, Helseth R, Ritschel V, Hansen CH, Andersen GO, Eritsland J, Halvorsen S, Fagerland MW, Solheim S, Arnesen H. et al. Double-stranded DNA and NETs components in relation to clinical outcome after ST-Elevation myocardial infarction. Sci Rep. 2020;10(1):5007. doi:10.1038/s41598-020-61971-7. Epub 2020/03/21. PubMed PMID: 32193509; PMCID: PMC7081350.
   PubMedGoogle Scholar
• Brown GD, Herre J, Williams DL, Willment JA, Marshall AS, Gordon S. Dectin-1 mediates the biological effects of beta-glucans. J Exp Med. 2003;197(9):1119–24. doi:10.1084/jem.20021890. Epub 2003/04/30. PubMed PMID: 12719478; PMCID: PMC2193964.
   PubMed Web of Science ®Google Scholar
• Yolanda H, Krajaejun T. Global distribution and clinical features of pythiosis in humans and animals. J Fungi (Basel). 2022;8(2):182. doi:10.3390/jof8020182. Epub 2022/02/26. PubMed PMID: 35205934; PMCID: PMC8879638.
   PubMedGoogle Scholar
• Kennedy RB, Ovsyannikova IG, Palese P, Poland GA. Current challenges in vaccinology. Front Immunol. 2020;11:1181. doi:10.3389/fimmu.2020.01181. Epub 2020/07/17. PubMed PMID: 32670279; PMCID: PMC7329983.
   PubMed Web of Science ®Google Scholar
• Yan Y, Nguyen LH, Franzosa EA, Huttenhower C. Strain-level epidemiology of microbial communities and the human microbiome. Genome Med. 2020;12(1):71. doi:10.1186/s13073-020-00765-y. Epub 2020/08/15. PubMed PMID: 32791981; PMCID: PMC7427293.
   PubMed Web of Science ®Google Scholar