Establishing functional lentiviral vector production in a stirred bioreactor for CAR-T cell therapy

References

• Kim DW, Cho JY. Recent advances in allogeneic CAR-T cells. Biomolecules. 2020;10:263.
   PubMed Web of Science ®Google Scholar
• McCarron A, Donnelley M, McIntyre C, et al. Challenges of up-scaling lentivirus production and processing. J Biotechnol. 2016;240:23–30.
   PubMed Web of Science ®Google Scholar
• How CW, Ong YS, Low SS, et al. How far have we explored fungi to fight cancer? Semin Cancer Biol. 2021. DOI:10.1016/j.semcancer.2021.03.009
   PubMed Web of Science ®Google Scholar
• Marin V, Stornaiuolo A, Piovan C, et al. RD-MolPack technology for the constitutive production of self-inactivating lentiviral vectors pseudotyped with the nontoxic RD114-TR envelope. Mol Ther Methods Clin Dev. 2016;3:16033.
   PubMed Web of Science ®Google Scholar
• Otsuji TG, Bin J, Yoshimura A, et al. A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production. Stem Cell Reports. 2014;2(5):734–745.
   PubMed Web of Science ®Google Scholar
• Bora M, Yousuf RW, Dhar P, et al. An overview of process intensification and thermo stabilization for upscaling of Peste des petits ruminants vaccines in view of global control and eradication. Virusdisease. 2018;29(3):285–296.
   PubMedGoogle Scholar
• Sheu J, Beltzer J, Fury B, et al. Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor. Mol Ther Methods Clin Dev. 2015;2:15020.
   PubMed Web of Science ®Google Scholar
• Nakamura K, Harada Y, Takahashi H, et al. Systematic evaluation of suspension MDCK cells, adherent MDCK cells, and LLC-MK2 cells for preparing influenza vaccine seed virus. Vaccine. 2019;37:6526–6534.
   PubMed Web of Science ®Google Scholar
• Beltran Paschoal JF, Patiño SS, Bernardino T, et al. Adaptation to serum-free culture of HEK 293T and Huh7.0 cells. BMC Proc. 2014;8:259.
   Google Scholar
• Lee DK, Park J, Seo DW. Suspension culture of Vero cells for the production of adenovirus type 5. Clin Exp Vaccine Res. 2020;9(1):48–55.
   PubMed Web of Science ®Google Scholar
• Hegedus A, Kavanagh Williamson M, Khan MB, et al. Evidence for altered glutamine metabolism in human immunodeficiency virus type 1 infected primary human CD4(+) T Cells. AIDS Res Hum Retroviruses. 2017;33(12):1236–1247.
   PubMed Web of Science ®Google Scholar
• Kuystermans D, Avesh M, Al-Rubeai M. Online flow cytometry for monitoring apoptosis in mammalian cell cultures as an application for process analytical technology. Cytotechnology. 2016;68(3):399–408.
   PubMed Web of Science ®Google Scholar
• Guy HM, McCloskey L, Lye GJ, et al. Characterization of lentiviral vector production using microwell suspension cultures of HEK293T-derived producer cells. Hum Gene Ther Methods. 2013;24(2):125–139.
   PubMed Web of Science ®Google Scholar
• Bessa MJ, Brandão F, Querido MM, et al. Optimization of the harvesting and freezing conditions of human cell lines for DNA damage analysis by the alkaline comet assay. Mutat Res. 2019;845:402994.
   PubMed Web of Science ®Google Scholar
• Wu SC, Huang GY, Liu JH. Production of retrovirus and adenovirus vectors for gene therapy: a comparative study using microcarrier and stationary cell culture. Biotechnol Prog. 2002;18(3):617–622.
   PubMed Web of Science ®Google Scholar
• Quintana-Bustamante O, Fañanas-Baquero S, Orman I, et al. Gene editing of PKLR gene in human hematopoietic progenitors through 5ʹ and 3ʹ UTR modified TALEN mRNA. PloS One. 2019;14(10):e0223775.
   PubMed Web of Science ®Google Scholar
• Choy MM, Sessions OM, Gubler DJ, et al. Production of infectious dengue virus in aedes aegypti is dependent on the ubiquitin proteasome pathway. PLoS Negl Trop Dis. 2015;9(11):e0004227.
   PubMed Web of Science ®Google Scholar
• Li Y, Li S, Li Y, et al. Generation of a novel HEK293 luciferase reporter cell line by CRISPR/Cas9-mediated site-specific integration in the genome to explore the transcriptional regulation of the PGRN gene. Bioengineered. 2019;10(1):98–107.
   PubMed Web of Science ®Google Scholar
• Lorenzo E, Méndez L, Rodríguez E, et al. Plasticity of the HEK-293 cells, related to the culture media, as platform to produce a subunit vaccine against classical swine fever virus. AMB Express. 2019;9(1):139.
   PubMed Web of Science ®Google Scholar
• Morris CD, Azadnia P, De Val N, et al. Differential antibody responses to conserved HIV-1 neutralizing epitopes in the context of multivalent scaffolds and native-like gp140 trimers. mBio. 2017;8(1):8.
   Web of Science ®Google Scholar
• Russell TA, Blizinsky KD, Cobia DJ, et al. A sequence variant in human KALRN impairs protein function and coincides with reduced cortical thickness. Nat Commun. 2014;5(1):4858.
   PubMedGoogle Scholar
• Li J, Lam M. Registered report: the microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44. eLife. 2015;4:e06434.
   PubMed Web of Science ®Google Scholar
• Yang S, Cao B, Zhou G, et al. Targeting B7-H3 immune checkpoint with chimeric antigen receptor-engineered natural killer cells exhibits potent Cytotoxicity against non-small cell lung cancer. Front Pharmacol. 2020;11:1089.
   PubMed Web of Science ®Google Scholar
• Kusena JWT, Shariatzadeh M, Thomas RJ, et al. Understanding cell culture dynamics: a tool for defining protocol parameters for improved processes and efficient manufacturing using human embryonic stem cells. Bioengineered. 2021;12(1):979–996.
   PubMed Web of Science ®Google Scholar
• Goh JS, Liu Y, Liu H, et al. Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function. Biotechnol J. 2014;9(1):100–109.
   PubMed Web of Science ®Google Scholar
• Wang L, Wang W, Xu Y, et al. Low levels of SPARC are associated with tumor progression and poor prognosis in Human Endometrial Carcinoma. Onco Targets Ther. 2020;13:11549–11569.
   PubMed Web of Science ®Google Scholar
• Hsiao F, Frouard J, Gramatica A, et al. Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection. PLoS Pathog. 2020;16(4):e1008450.
   PubMed Web of Science ®Google Scholar
• Gallo-Ramírez LE, Nikolay A, Genzel Y, et al. Bioreactor concepts for cell culture-based viral vaccine production. Expert Rev Vaccines. 2015;14(9):1181–1195.
   PubMed Web of Science ®Google Scholar
• De Los Milagros Bassani Molinas M, Beer C, Hesse F, et al. Optimizing the transient transfection process of HEK-293 suspension cells for protein production by nucleotide ratio monitoring. Cytotechnology. 2014;66(3):493–514.
   PubMed Web of Science ®Google Scholar
• Humbert O, Gisch DW, Wohlfahrt ME, et al. Development of third-generation cocal envelope producer cell lines for robust lentiviral gene transfer into Hematopoietic Stem Cells and T-cells. Mol Ther. 2016;24(7):1237–1246.
   PubMed Web of Science ®Google Scholar
• Frey S, Vesikari T, Szymczakiewicz-Multanowska A, et al. Clinical efficacy of cell culture–derived and egg‐derived inactivated subunit influenza vaccines in healthy adults. Clin Infect Dis. 2010;51(9):997–1004.
   PubMed Web of Science ®Google Scholar
• Warnock JN, Al-Rubeai M. Bioreactor systems for the production of biopharmaceuticals from animal cells. Biotechnol Appl Biochem. 2006;45(1):1–12.
   PubMed Web of Science ®Google Scholar
• Costariol E, Rotondi M, Amini A, et al. Establishing the scalable manufacture of primary human T-cells in an automated stirred-tank bioreactor. Biotechnol Bioeng. 2019;116(10):2488–2502.
   PubMed Web of Science ®Google Scholar
• Hsu CY, Uludağ H. A simple and rapid nonviral approach to efficiently transfect primary tissue-derived cells using polyethylenimine. Nat Protoc. 2012;7(5):935–945.
   PubMed Web of Science ®Google Scholar
• Bauler M, Roberts JK, Wu CC, et al. Production of Lentiviral vectors using suspension cells grown in serum-free media. Mol Ther Methods Clin Dev. 2020;17:58–68.
   PubMed Web of Science ®Google Scholar