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Research Paper

An easy method for developing fusion enabled SARS-CoV2 virus fusion mimic (SCFM), bypassing the need of Bio Safety Level (BSL) facility

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Pages 4407-4419 | Received 10 Mar 2021, Accepted 17 Jun 2021, Published online: 26 Aug 2021

References

  • Dennison Himmelfarb CR, Baptiste D. Coronavirus Disease (COVID-19). J Cardiovasc Nurs. 2020;35:318–321.
  • Chan JFW, Kok KH, Zhu Z, et al. Genomic characterization of the 2019 novelhuman-pathogenic coronavirus isolated from a patient with atypical pneumonia after visitingWuhan. Emerg Microbes Infect. 2020;9:221–236.
  • Jiang S, He Y, Liu S. SARS vaccinedevelopment. Emerg Infect Dis. 2005;11:7–11.
  • Du L, He Y, Zhou Y, et al. The spike protein ofSARS-CoV - A target for vaccine and therapeuticdevelopment. Nat Rev Microbiol. 2009;7:226–236.
  • Darnell MER, Taylor DR. Evaluation of inactivation methods for severe acute respiratory syndrome coronavirus in noncellular bloodproducts. Transfusion. 2006;46:1770–1777.
  • Walls AC, Park YJ, Tortorici MA, et al. Structure, function, and antigenicity of theSARS-CoV-2 spikeglycoprotein. Cell. 2020;181:281–292.e6.
  • Nie J, Li Q, Wu J, et al. Establishment and validation of a pseudovirus neutralization assay forSARS-CoV-2. Emerg Microbes Infect. 2020;9:680–686.
  • Darnell MER, Subbarao K, Feinstone SM, et al. Inactivation of the coronavirus that induces severe acute respiratory syndrome,SARS-CoV. J Virol Methods. 2004;121:85–91.
  • Lorizate M, Kräusslich HG. Role of lipids in virusreplication. Cold Spring Harb Perspect Biol. 2011;3:1–20.
  • Ganguli N, Ganguli N, Chandra S, et al. A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline geneintegration. Appl Microbiol Biotechnol. 2018;102:6221–6234.
  • (1) Molecular Cloning: A Laboratory Manual (3-Volume Set) [Internet]. [ cited 2021 Jan 2]; Available from: https://www.researchgate.net/publication/200037138_Molecular_Cloning_A_Laboratory_Manual_3-Volume_Set
  • Steinberg F, Gerber SD, Rieckmann T, et al. Rapid fusion and syncytium formation of heterologous cells upon expression of the FGFRL1receptor. J Biol Chem. 2010;285:37704–37715.
  • Jackson RJ, Howell MT, Kaminski A. The novel mechanism of initiation of picornavirus RNAtranslation. Trends Biochem Sci. 1990;15:477–483.
  • Hofmann H, Pöhlmann S. Cellular entry of the SARScoronavirus. Trends Microbiol. 2004;12:466–472.
  • Chakraborti S, Prabakaran P, Xiao X, et al. The SARS coronavirus S glycoprotein receptor binding domain: fine mapping and functionalcharacterization. Virol J. 2005;2:73.
  • Belouzard S, Millet JK, Licitra BN, et al. Mechanisms of coronavirus cell entry mediated by the viral spikeprotein. Viruses. 2012;4:1011–1033.
  • Saginario C, Sterling H, Beckers C, et al. MFR, a putative receptor mediating the fusion ofmacrophages. Mol Cell Biol. 1998;18:6213–6223.
  • Han X, Sterling H, Chen Y, et al. CD47, a ligand for the macrophage fusion receptor, participates in macrophagemultinucleation. J Biol Chem. 2000;275:37984–37992.
  • Li W, Moore MJ, Vasllieva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARScoronavirus. Nature. 2003;426:450–454.
  • Mossel EC, Huang C, Narayanan K, et al. Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirusreplication. J Virol. 2005;79:3846–3850.
  • Williams K, Chubb C, Huberman E, et al. Analysis of differential protein expression in normal and neoplastic human breast epithelial celllines. Electrophoresis. 1998;19:333–343.
  • Jeyanathan M, Afkhami S, Smaill F, et al. Immunological considerations forCOVID-19 vaccinestrategies. Nat Rev Immunol [Internet]. 2020;20:615–632.
  • Hu J, Gao Q, He C, et al. Development ofcell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies againstSARS-CoV-2. Genes Dis. 2020;7:551–557.
  • Li Q, Liu Q, Huang W, et al. Current status on the development of pseudoviruses for envelopedviruses. Rev Med Virol. 2018;28:e1963.
  • Xia S, Liu M, Wang C, et al. Inhibition ofSARS-CoV-2 (previously2019-nCoV) infection by a highly potentpan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membranefusion. Cell Res [Internet] 2020 [cited 2021 June 15];30:343–355.
  • Theuerkauf SA, Michels A, Riechert V, et al. Quantitative assays reveal cell fusion at minimal levels ofSARS-CoV-2 spike protein and fusion fromwithout. iScience. 2021;24:102170.
  • Papa G, Mallery DL, Albecka A, et al. Furin cleavage ofSARS-CoV-2 Spike promotes but is not essential for infection andcell-cellfusion. PLoS Pathog [Internet] 2021 [cited 2021 June 15];17:e1009246.
  • Peter AP, Wayne CK, Show PL, et al. Potential Pathway that Could Treat Coronaviruses(COVID-19). Curr Biochem Eng. 2020;6: 3–4.
  • Chia WY, Kok H, Chew KW, et al. Can algae contribute to the war withCovid-19? Bioengineered [Internet]. 2021;12:1226–1237.
  • Ahadu E. Novel corona virusCovid-19: impact on economic development and mitigating solution for developingcountries. Humanit Soc Sci. 2020;8:86.