Using pgRNA–Cas9 system to knockout MKL1 inhibited cell migration and proliferation

References

• Asparuhova, M. B., Ferralli, J., Chiquet, M., & Chiquet-Ehrismann, R. (2011). The transcriptional regulator megakaryoblastic leukemia-1 mediates serum response factor-independent activation of tenascin-C transcription by mechanical stress. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, 25, 3477–3488. doi:10.1096/fj.11-187310
   PubMed Web of Science ®Google Scholar
• Bell, J. L., Haak, A. J., Wade, S. M., Sun, Y., Neubig, R. R., & Larsen, S. D. (2013). Design and synthesis of tag-free photoprobes for the identification of the molecular target for CCG-1423, a novel inhibitor of the Rho/MKL1/SRF signaling pathway. Beilstein Journal of Organic Chemistry, 9, 966–973. doi:10.3762/bjoc.9.111
   PubMed Web of Science ®Google Scholar
• Berns, E. J., Alvarez, Z., Goldberger, J. E., Boekhoven, J., Kessler, J. A., Kuhn, H. G., & Stupp, S. I. (2016). A tenascin-C mimetic peptide amphiphile nanofiber gel promotes neurite outgrowth and cell migration of neurosphere-derived cells. Acta biomaterialia, 37, 50–58. doi:10.1016/j.actbio.2016.04.010
   PubMed Web of Science ®Google Scholar
• Bhaya, D., Davison, M., & Barrangou, R. (2011). CRISPR-Cas systems in bacteria and archaea: Versatile small RNAs for adaptive defense and regulation. Annual Review of Genetics, 45, 273–297. doi:10.1146/annurev-genet-110410-132430
   PubMed Web of Science ®Google Scholar
• Cazzalini, O., Scovassi, A. I., Savio, M., Stivala, L. A., & Prosperi, E. (2010). Multiple roles of the cell cycle inhibitor p21(CDKN1A) in the DNA damage response. Mutation Research, 704, 12–20. doi:10.1016/j.mrrev.2010.01.009
   PubMed Web of Science ®Google Scholar
• Cen, B., Selvaraj, A., & Prywes, R. (2004). Myocardin/MKL family of SRF coactivators: Key regulators of immediate early and muscle specific gene expression. Journal of Cellular Biochemistry, 93, 74–82. doi:10.1002/jcb.20199
   PubMed Web of Science ®Google Scholar
• Cheng, X., Yang, Y., Fan, Z., Yu, L., Bai, H., Zhou, B., … Xu, Y. (2015). MKL1 potentiates lung cancer cell migration and invasion by epigenetically activating MMP9 transcription. Oncogene, 34, 5570–5581. doi:10.1038/onc.2015.14
   PubMed Web of Science ®Google Scholar
• Descot, A., Hoffmann, R., Shaposhnikov, D., Reschke, M., Ullrich, A., & Posern, G. (2009). Negative regulation of the EGFR-MAPK cascade by actin-MAL-mediated Mig6/Errfi-1 induction. Molecular Cell, 35, 291–304. doi:10.1016/j.molcel.2009.07.015
   PubMed Web of Science ®Google Scholar
• Duggirala, A., Kimura, T. E., Sala-Newby, G. B., Johnson, J. L., Wu, Y. J., Newby, A. C., & Bond, M. (2015). cAMP-induced actin cytoskeleton remodelling inhibits MKL1-dependent expression of the chemotactic and pro-proliferative factor, CCN1. Journal of Molecular and Cellular Cardiology, 79, 157–168. doi:10.1016/j.yjmcc.2014.11.012
   PubMed Web of Science ®Google Scholar
• Emre, Y., & Imhof, B. A. (2014). Matricellular protein CCN1/CYR61: A new player in inflammation and leukocyte trafficking. Seminars in Immunopathology, 36, 253–259. doi:10.1007/s00281-014-0420-1
   PubMed Web of Science ®Google Scholar
• Feliciello, I., & Chinali, G. (1993). A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli. Anal Biochem, 212, 394–401. doi:10.1006/abio.1993.1346
   PubMed Web of Science ®Google Scholar
• Gaj, T., Gersbach, C. A., & Barbas, C. F., 3rd. (2013). ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in Biotechnology, 31, 397–405. doi:10.1016/j.tibtech.2013.04.004
   PubMed Web of Science ®Google Scholar
• Gurbuz, I., Ferralli, J., Roloff, T., Chiquet-Ehrismann, R., & Asparuhova, M. B. (2014). SAP domain-dependent Mkl1 signaling stimulates proliferation and cell migration by induction of a distinct gene set indicative of poor prognosis in breast cancer patients. Molecular Cancer, 13, 22. doi:10.1186/1476-4598-13-22
   PubMed Web of Science ®Google Scholar
• Hampl, V., Martin, C., Aigner, A., Hoebel, S., Singer, S., Frank, N., … Muehlich, S. (2013). Depletion of the transcriptional coactivators megakaryoblastic leukaemia 1 and 2 abolishes hepatocellular carcinoma xenograft growth by inducing oncogene-induced senescence. EMBO Molecular Medicine, 5, 1367–1382. doi:10.1002/emmm.201202406
   PubMed Web of Science ®Google Scholar
• Haussecker, D. (2014). Current issues of RNAi therapeutics delivery and development. Journal of Controlled Release: Official Journal of the Controlled Release Society, 195, 49–54. doi:10.1016/j.jconrel.2014.07.056
   PubMed Web of Science ®Google Scholar
• Hopkins, S., Linderoth, E., Hantschel, O., Suarez-Henriques, P., Pilia, G., Kendrick, H., … Ferby, I. (2012). Mig6 is a sensor of EGF receptor inactivation that directly activates c-Abl to induce apoptosis during epithelial homeostasis. Developmental Cell, 23, 547–559. doi:10.1016/j.devcel.2012.08.001
   PubMed Web of Science ®Google Scholar
• Hutcheson, J., Witkiewicz, A. K., & Knudsen, E. S. (2015). The RB tumor suppressor at the intersection of proliferation and immunity: Relevance to disease immune evasion and immunotherapy. Cell Cycle, 14, 3812–3819. doi:10.1080/15384101.2015.1010922
   PubMed Web of Science ®Google Scholar
• Kircher, P., Hermanns, C., Nossek, M., Drexler, M. K., Grosse, R., Fischer, M., … Muehlich, S. (2015). Filamin A interacts with the coactivator MKL1 to promote the activity of the transcription factor SRF and cell migration. Science Signaling, 8, ra112. doi:10.1126/scisignal.aad2959
   PubMed Web of Science ®Google Scholar
• Koo, T., Lee, J., & Kim, J. S. (2015). Measuring and reducing off-target activities of programmable nucleases including CRISPR-Cas9. Molecules and Cells, 38, 475–481. doi:10.14348/molcells.2015.0103
   PubMed Web of Science ®Google Scholar
• Liao, X. H., Wang, N., Liu, L. Y., Zheng, L., Xing, W. J., Zhao, D. W., … Zhang, T. C. (2014). MRTF-A and STAT3 synergistically promote breast cancer cell migration. Cellular Signalling, 26, 2370–2380. doi:10.1016/j.cellsig.2014.07.023
   PubMed Web of Science ®Google Scholar
• Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., … Church, G. M. (2013). RNA-guided human genome engineering via Cas9. Science, 339, 823–826. doi:10.1126/science.1232033
   PubMed Web of Science ®Google Scholar
• McCaffrey, J., Sibert, J., Zhang, B., Zhang, Y., Hu, W., Riethman, H., & Xiao, M. (2016). CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis. Nucleic Acids Research, 44, e11. doi:10.1093/nar/gkv878
   PubMed Web of Science ®Google Scholar
• Meek, D. W. (2015). Regulation of the p53 response and its relationship to cancer. The Biochemical Journal, 469, 325–346. doi:10.1042/BJ20150517
   PubMed Web of Science ®Google Scholar
• Nobusue, H., Onishi, N., Shimizu, T., Sugihara, E., Oki, Y., Sumikawa, Y., … Kano, K. (2014). Regulation of MKL1 via actin cytoskeleton dynamics drives adipocyte differentiation. Nature Communications, 5, 3368. doi:10.1038/ncomms5972
   PubMed Web of Science ®Google Scholar
• Schaal, C., Pillai, S., & Chellappan, S. P. (2014). The Rb-E2F transcriptional regulatory pathway in tumor angiogenesis and metastasis. Adv Cancer Res, 121, 147–182. doi:10.1016/B978-0-12-800249-0.00004-4
   PubMed Web of Science ®Google Scholar
• Scharenberg, M. A., Chiquet-Ehrismann, R., & Asparuhova, M. B. (2010). Megakaryoblastic leukemia protein-1 (MKL1): Increasing evidence for an involvement in cancer progression and metastasis. The International Journal of Biochemistry & Cell Biology, 42, 1911–1914. doi:10.1016/j.biocel.2010.08.014
   PubMed Web of Science ®Google Scholar
• Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., … Zhang, F. (2014). Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 343, 84–87. doi:10.1126/science.1247005
   PubMed Web of Science ®Google Scholar
• Smith, E. C., Teixeira, A. M., Chen, R. C., Wang, L., Gao, Y., Hahn, K. L., & Krause, D. S. (2013). Induction of megakaryocyte differentiation drives nuclear accumulation and transcriptional function of MKL1 via actin polymerization and RhoA activation. Blood, 121, 1094–1101. doi:10.1182/blood-2012-05-429993
   PubMed Web of Science ®Google Scholar
• Terns, R. M., & Terns, M. P. (2014). CRISPR-based technologies: Prokaryotic defense weapons repurposed. Trends in Genetics: TIG, 30, 111–118. doi:10.1016/j.tig.2014.01.003
   PubMed Web of Science ®Google Scholar
• Unniyampurath, U., Pilankatta, R., & Krishnan, M. N. (2016). RNA Interference in the Age of CRISPR: Will CRISPR Interfere with RNAi? International Journal of Molecular Sciences, 17, 291. doi:10.3390/ijms17030291
   PubMed Web of Science ®Google Scholar
• Wong, C. C., Wong, C. M., Ko, F. C., Chan, L. K., Ching, Y. P., Yam, J. W., & Ng, I. O. (2008). Deleted in liver cancer 1 (DLC1) negatively regulates Rho/ROCK/MLC pathway in hepatocellular carcinoma. PloS one, 3, e2779. doi:10.1371/journal.pone.0002779
   PubMed Web of Science ®Google Scholar
• Xing, W. J., Liao, X. H., Wang, N., Zhao, D. W., Zheng, L., Zheng, D. L., … Zhang, T. C. (2015). MRTF-A and STAT3 promote MDA-MB-231 cell migration via hypermethylating BRSM1. IUBMB Life, 67, 202–217. doi:10.1002/iub.1362
   PubMed Web of Science ®Google Scholar
• Yang, S., Liu, L., Xu, P., & Yang, Z. (2015). MKL1 inhibits cell cycle progression through p21 in podocytes. BMC Molecular Biology, 16, 1. doi:10.1186/s12867-015-0029-5
   PubMed Web of Science ®Google Scholar
• Zhu, S., Li, W., Liu, J., Chen, C. H., Liao, Q., Xu, P., … Wei, W. (2016). Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Nature Biotechnology, 34, 1279–1286. doi:10.1038/nbt.3715
   PubMed Web of Science ®Google Scholar