The first records of Niphargus hrabei and N. potamophilus in Ukraine and Bulgaria significantly enlarge the ranges of these species

References

• Astrin JJ, Stüben PE. 2008. Phylogeny in cryptic weevils: Molecules, morphology and new genera of western Palaearctic Cryptorhynchinae (Coleoptera: Curculionidae). Invertebrate Systematics 22(5):503–522. DOI: 10.1071/IS07057.
   Web of Science ®Google Scholar
• Badertscher S, Fleitmann D, Cheng H, Edwards RL, Göktürk OM, Zumbühl A, Leuenberger M, Tüysüz O. 2011. Pleistocene water intrusions from the Mediterranean and Caspian Seas into the Black Sea. Nature Geoscience 4(4):236–239. DOI: 10.1038/ngeo1106.
   Web of Science ®Google Scholar
• Bahr A, Arz HW, Lamy F, Wefer G. 2006. Late glacial to Holocene paleoenvironmental evolution of the Black Sea, reconstructed with stable oxygen isotope records obtained on ostracod shells. Earth and Planetary Scientific Letters 241(3–4):863–875. DOI: 10.1016/j.epsl.2005.10.036.
   Web of Science ®Google Scholar
• Birstein JA. 1954. Report of epigean Niphargus (Crustacea, Amphipoda) in the Don delta and Kuban basin. Zoologicheskyi Zhurnal 33:1025–1031. In Russian.
   Google Scholar
• Birštein JA. 1940. To the fauna of cave-dwelling amphipods of Abkhazia. Byulleten Moskovskogo obshchestva ispytatieley prirody. Otdel Biologicheskii 49(3–4):47–55. In Russian.
   Google Scholar
• Birštein JA. 1952. Subterranean amphipods of the Khosta–Gudauta region (western Transcaucasia). Byulleten Moskovskogo Obshchestva Ispytatieley Prirody. Otdel Biologicheskii 57(1):26–39. In Russian.
   Google Scholar
• Borko Š, Trontelj P, Seehausen O, Moškrič A, Fišer C. 2021. A subterranean adaptive radiation of amphipods in Europe. Nature Communications 12(1):3688. DOI: 10.1038/s41467-021-24023-w.
   Web of Science ®Google Scholar
• Cărăușu S, Dobreanu E, Manolache C. 1955. Amphipoda. Fauna Republicii Populare Romine. Crustacea 4:92–96.
   Google Scholar
• Casquet J, Thebaud C, Gillespie RG. 2012. Chelex without boiling, a rapid and easy technique to obtain stable amplifiable DNA from small amounts of ethanol-stored spiders. Molecular Ecology Resources 12(1):136–141. DOI: 10.1111/j.1755-0998.2011.03073.x.
   PubMed Web of Science ®Google Scholar
• Chepalyga AL. 2007. The late glacial great flood in the Ponto-Caspian basin. In: Yanko-Hombach V, Gilbert AS, Panin N, Dolukhanov PM, editors. The Black Sea flood question: Changes in coastline, climate and human settlement. Dordrecht: Springer. pp. 119–148.
   Google Scholar
• Copilaș-Ciocianu D, Fišer C, Borza P, Balázs G, Angyal D, Petrusek A. 2017. Low intraspecific genetic divergence and weak niche differentiation despite wide ranges and extensive sympatry in two epigean Niphargus species (Crustacea: Amphipoda). Zoological Journal of the Linnean Society 181(3):485–499. DOI: 10.1093/zoolinnean/zlw031.
   Web of Science ®Google Scholar
• Copilaş-Ciocianu D, Fišer C, Borza P, Petrusek A. 2018. Is subterranean lifestyle reversible? Independent and recent large-scale dispersal into surface waters by two species of the groundwater amphipod genus Niphargus. Molecular Phylogenetics and Evolution 119:37–49. DOI: 10.1016/j.ympev.2017.10.023.
   PubMed Web of Science ®Google Scholar
• Copilaş-Ciocianu D, Grabowski M, Pârvulescu L, Petrusek A. 2014. Zoogeography of epigean freshwater Amphipoda (Crustacea) in Romania: Fragmented distributions and wide altitudinal variability. Zootaxa 3893(2):243–260. DOI: 10.11646/zootaxa.3893.2.5.
   Web of Science ®Google Scholar
• Copilaş-Ciocianu D, Rewicz T, Sands AF, Palatov D, Marin I, Arbačiauskas K, Hebert PDN, Grabowski M, Audzijonyte A. 2022. A DNA barcode reference library for endemic Ponto-Caspian amphipods. Scientific Reports 12(1):11332. DOI: 10.1038/s41598-022-15442-w.
   Web of Science ®Google Scholar
• Dedju I. 1967. Amphipods and mizids of basins of Dniester and Prut rivers. Moscow: Nauka. p. 172. In Russian.
   Google Scholar
• Dudich E. 1941. Niphargus aus einer Therme von Budapest. Annales Musei Nationalis Hungarici. Pars zoologica 34:165–176. In German.
   Google Scholar
• Edgar RC. 2004. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32(5):1792–1797. DOI: 10.1093/nar/gkh340.
   PubMed Web of Science ®Google Scholar
• Federov PV. 1971. Postglacial transgression of the Black Sea. International Geology Review 14(2):160–164. DOI: 10.1080/00206817209475678.
   Google Scholar
• Fišer C. 2012. Niphargus: A model system for evolution and ecology. In: Culver DC, White WB, editors. Encyclopedia of caves. New York: Academic Press. pp. 555–564.
   Google Scholar
• Flot JF, Bauermeister J, Brad T, Hillebrand‐Voiculescu A, Sarbu SM, Dattagupta S. 2014. Niphargus–Thiothrix associations may be widespread in sulphidic groundwater ecosystems: Evidence from southeastern Romania. Molecular Ecology 23(6):1405–1417. DOI: 10.1111/mec.12461.
   Web of Science ®Google Scholar
• Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5):294–299. PMID: 7881515.
   PubMedGoogle Scholar
• Georgievski G, Stanev EV. 2006. Paleo-evolution of the Black Sea watershed: Sea level and water transport through the Bosporus Straits as an indicator of the Lateglacial-Holocene transition. Climate Dynamics 26(6):631–644. DOI: 10.1007/s00382-006-0123-y.
   Web of Science ®Google Scholar
• Gogaladze A, Son MO, Lattuada M, Anistratenko VV SVL, Pavel AB, Popa OP, Popa LO, ter Poorten J-J, Biesmeijer JC, Raes N, Wilke T, Sands AF, Trichkova T, Hubenov ZK, Vinarski MV, Anistratenko OY, Alexenko TL, Wesselingh FP. 2021. Decline of unique Pontocaspian biodiversity in the Black Sea Basin: A review. Ecology and Evolution 11(19):12923–12947. DOI: 10.1002/ece3.8022.
   Web of Science ®Google Scholar
• Hebert PD, Cywinska A, Ball SL, Dewaard JR. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences 270(1512):313–321. DOI: 10.1098/rspb.2002.2218.
   Google Scholar
• Horton T, Lowry J, De Broyer C, Bellan-Santini D, Coleman CO, Corbari L, Costello MJ, Daneliya M, Dauvin J-C, Fišer C, Gasca R, Grabowski M, Guerra-García JM, Hendrycks E, Hughes L, Jaume D, Jazdzewski K, Kim Y-H, King R, Krapp-Schickel T, LeCroy S, Lörz A-N, Mamos T, Senna AR, Serejo C, Sket B, Souza-Filho JF, Tandberg AH, Thomas JD, Thurston M, Vader W, Väinölä R, Vonk R, White K, Zeidler W. 2022. World Amphipoda Database. DOI: 10.14284/368.
   Google Scholar
• Hou ZG, Fu JH, Li SQ. 2007. A molecular phylogeny of the genus Gammarus (Crustacea: Amphipoda) based on mito-chondrial and nuclear gene sequences. Molecular Phylogenetics and Evolution 45(2):596–611. DOI: 10.1016/j.ympev.2007.06.006.
   Web of Science ®Google Scholar
• Karaman S. 1950. Etudes sur les Amphipodes-Isopodes des Balkans. Belgrade: Academie Serbe des Sciences Monographies.
   Google Scholar
• Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16(2):111–120. DOI: 10.1007/BF01731581.
   PubMed Web of Science ®Google Scholar
• Lefébure T, Douady CJ, Gouy M, Trontelj P, Briolay J, Gibert J. 2006. Phylogeography of a subterranean amphipod reveals cryptic diversity and dynamic evolution in extreme environments. Molecular Ecology 15(7):1797–1806. DOI: 10.1111/j.1365-294X.2006.02888.x.
   Web of Science ®Google Scholar
• Lefébure T, Douady CJ, Malard F, Gibert J. 2007. Testing dispersal and cryptic diversity in a widely distributed groundwater amphipod (Niphargus rhenorhodanensis). Molecular Phylogenetics and Evolution 42(3):676–686. DOI: 10.1016/j.ympev.2006.08.020.
   PubMed Web of Science ®Google Scholar
• Leigh JW, Bryant D. 2015. popart: Full-feature software for haplotype network construction. Methods in Ecology and Evolution 6(9):1110–1116. DOI: 10.1111/2041-210X.12410.
   Web of Science ®Google Scholar
• Librado P, Rozas J. 2009. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25(11):1451–1452. DOI: 10.1093/bioinformatics/btp187.
   PubMed Web of Science ®Google Scholar
• Meijering MPD, Jazdzewski K, Kohn J. 1995. Ecotypes of Amphipoda in Central European inland waters. Polskie Archiwum Hydrobiologii 42:527–536.
   Google Scholar
• Morhun H, Copilas-Ciocianu D, Rewicz T, Son MO, Khomenko A, Huseynov M, Utecsky S, Grabowski M. 2022. Molecular markers and SEM imaging reveal pseudocryptic diversity within the Ponto-Caspian low-profile amphipod invader Dikerogammarus bispinosus. The European Zoological Journal 89(1):87–101. DOI: 10.1080/24750263.2021.2018056.
   Web of Science ®Google Scholar
• Nesemann H, Pöckl M, Wittmann KJ. 1995. Distribution of epigean Malacostraca in the middle and upper Danube (Hungary, Austria, Germany). Miscellanea Zoologica Hungarica 10:49–68.
   Google Scholar
• Olenkovsky M. 2012. Neolithic of the Sivash region. Kherson: Aylant. p. 176. In Russian.
   Google Scholar
• Palatov D, Marin I. 2021. Epigean (pond-dwelling) species of the genus Niphargus Schiödte, 1849 (Crustacea: Amphipoda: Niphargidae) from the coastal plains of the Black and Azov seas of the north- and south-western Caucasus. Invertebrate Zoology 18(2):105–151. DOI: 10.15298/invertzool.18.2.05.
   Google Scholar
• Pérez-Moreno JL, Balázs G, Wilkins B, Herczeg G, Bracken-Grissom HD. 2017. The role of isolation on contrasting phylogeographic patterns in two cave crustaceans. BMC Evolutionary Biology 17(1):247. DOI: 10.1186/s12862-017-1094-9.
   Google Scholar
• Ratnasingham S, Hebert PD. 2007. BOLD: The Barcode of Life Data System. Molecular Ecology Resources 7:355–364. http://www.barcodinglife.org
   PubMed Web of Science ®Google Scholar
• Ratnasingham S, Hebert PD. 2013. A DNA-based registry for all animal species: The Barcode Index Number (BIN) system. PloS ONE 8(7):e66213. DOI: 10.1371/journal.pone.0066213.
   PubMed Web of Science ®Google Scholar
• Ryan WBF, Pitman IIIWC, Major CO, Shimkus K, Moskalenko V, Jones GA, Dimitrov P, Gorür N, Sakinç M, Yüce H. 1997. An abrupt drowning of the Black Sea shelf. Marine Geology 138(1–2):119–126. DOI: 10.1016/S0025-3227(97)00007-8.
   Web of Science ®Google Scholar
• Sket B. 1981. Distribution, Ecological, Character and Phylogenetic importance of Niphargus valachicus (Amphipoda, Gammaridae, S.L.). Biology Vestnik 29(1):87–103. In Slovenian.
   Google Scholar
• Straškraba M. 1972. Les groupments des especes du genre Niphargus (sensu lato). Actes du Ier Colloque International sur le genre Niphargus. Museo Civico di Storia Naturale di Verona Memorie fuori Serie 5:85–90.
   Google Scholar
• Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30(12):2725–2729. DOI: 10.1093/molbev/mst197.
   PubMed Web of Science ®Google Scholar
• Trontelj P, Douady C, Fišer C, Gibert J, Š G, Lefébure T, Sket B, Zakšek V. 2009. A molecular test for hidden biodiversity in groundwater: How large are the ranges of macro-stygobionts? Freshwater Biology 54(4):727–744. DOI: 10.1111/j.1365-2427.2007.01877.x.
   Web of Science ®Google Scholar
• Yanko-Hombach V, Yanina T. 2019. Toward an understanding of human responses to environmental change in the Caspian-Black Sea-Mediterranean Corridors (IGCP 610 final report). Episodes Journal of International Geoscience 42(4):343–354.
   Web of Science ®Google Scholar
• Yanko-Homback V, Schnyukov E, Pasynkov A, Sorokin V, Kuprin P, Maslakov N, Montnenko I, Smyntyna O. 2017. Geological and geomorphological factors and marine conditions of the Azov-Black Sea Basin and coastal characteristics as they determine prospecting for seabed prehistoric sites on the continental shelf. In: Flemming NC, Harff J, Moura D, Burgess A, Bailey GN, editors. Submerged landscapes of the European continental shelf: Quaternary Paleoenvironments, first edition. Oxford: Wiley Blackwell. pp. 431–478.
   Google Scholar
• Zorina-Sakharova K. 2017. Niphargus valachicus Dobreanu et Manolache (Amphipoda, Niphargidae) in Kiliya Delta of the Danube River. Hydrobiological Journal 53(5):46–56. DOI: 10.1615/HydrobJ.v53.i5.50.
   Google Scholar