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Research Paper

Inequality and allergenic cover of urban greenspaces surrounding public elementary schools in Vancouver, British Columbia, Canada

, &
Pages 178-190 | Received 18 Aug 2020, Accepted 10 Mar 2021, Published online: 07 Apr 2021

References

  • Akcakaya N, Cokugras H, Camcioglu Y, Ozdemir M. 2005. Skin test hypersensitivity for childhood asthma in Istanbul during a period of 16 years. Allergologia et Immunopathologia. 33(1):15–19. doi:10.1157/13070603.
  • Allen JRL, Dark P. 2008. Seasonality of modern pollen and sediment deposition in an estuarine context: the Severn Estuary Levels, southwest England. J Quat Sci. 23(3):213–228. doi:10.1002/jqs.1138.
  • Avolio ML, Pataki DE, Gillespie TW, Jenerette GD, McCarthy HR, Pincetl S, Clarke LW. 2015. Tree diversity in southern California’s urban forest: the interacting roles of social and environmental variables. Front Ecol Evol. 3(JUL). doi:10.3389/fevo.2015.00073.
  • Barton J, Griffin M, Pretty J. 2012. Exercise-, nature- and socially interactive-based initiatives improve mood and self-esteem in the clinical population. Perspect Public Health. 132(2):89–96. doi:10.1177/1757913910393862.
  • BC Ministry of Education. 2009. Schools. City of Vancouver Open Data Portal. https://opendata.vancouver.ca/explore/dataset/schools/information.
  • Beckett KP, Freer-Smith PH, Taylor G. 2000. Particulate pollution capture by urban trees: effect of species and windspeed. Glob Chang Biol. 6(8):995–1003. doi:10.1046/j.1365-2486.2000.00376.x.
  • Berman MG, Kross E, Krpan KM, Askren MK, Burson A, Deldin PJ, Kaplan S, Sherdell L, Gotlib IH, Jonides J, et al. 2012. Interacting with nature improves cognition and affect for individuals with depression. J Affect Disord. 140(3):300–305. doi:10.1016/j.jad.2012.03.012.
  • Bigirimana J, Bogaert J, De Cannière C, Bigendako MJ, Parmentier I. 2012. Domestic garden plant diversity in Bujumbura, Burundi: role of the socio-economical status of the neighbourhood and alien species invasion risk. Landsc Urban Plan. 107(2):118–126. doi:10.1016/j.landurbplan.2012.05.008.
  • Bonferroni CE. 1936. Teoria statistica delle classi e calcolo delle probabilità. Pubblicazioni Del R Istituto Superiore Di Scienze Economiche e Commerciali Di Firenze. 8:3–62.
  • Burbach GJ, Heinzerling LM, Edenharter G, Bachert C, Bindslev-Jensen C, Bonini S, Bousquet J, Bousquet-Rouanet L, Bousquet PJ, Bresciani M, et al. 2009. GA2 LEN skin test study II: clinical relevance of inhalant allergen sensitizations in Europe. Allergy: Europ J Allergy Clin Immunol. 64(10):1507–1515. doi:10.1111/j.1398-9995.2009.02089.x.
  • Calabria CW, Dice J. 2007. Aeroallergen sensitization rates in military children with rhinitis symptoms. Annals Allergy, Asthma Immunol. 99(2):161–169. doi:10.1016/S1081-1206(10)60640-0.
  • Cariñanos P, Adinolfi C, Díaz De La Guardia C, De Linares C, Casares-Porcel M. 2016. Characterization of allergen emission sources in urban areas. J Environ Qual. 45(1):244–252. doi:10.2134/jeq2015.02.0075.
  • Cariñanos P, Casares-Porcel M. 2011. Urban green zones and related pollen allergy: a review. Some guidelines for designing spaces with low allergy impact. Landsc Urban Plan. 101(3):205–214. doi:10.1016/j.landurbplan.2011.03.006.
  • Cariñanos P, Casares-Porcel M, Quesada-Rubio JM. 2014. Estimating the allergenic potential of urban green spaces: a case-study in Granada, Spain. Landsc Urban Plan. 123:134–144. doi:10.1016/j.landurbplan.2013.12.009.
  • Cebrino J, Galán C, Domínguez-Vilches E. 2016. Aerobiological and phenological study of the main Poaceae species in Córdoba city (Spain) and the surrounding hills. Aerobiologia. 32(4):595–606. doi:10.1007/s10453-016-9434-6.
  • City of Vancouver. 2012. Street-trees. City of Vancouver Open Data Portal. https://opendata.vancouver.ca/explore/dataset/street-trees/information/?disjunctive.species_name&disjunctive.common_name&disjunctive.height_range_id.
  • City of Vancouver. 2015. Greenest city action plan part two: 2015–2020. City of Vancouver. https://vancouver.ca/files/cov/greenest-city-2020-action-plan-2015-2020.pdf.
  • City of Vancouver. 2016. Population. City of Vancouver. https://vancouver.ca/news-calendar/population.aspx?.
  • Clarke LW, Jenerette GD, Davila A. 2013. The luxury of vegetation and the legacy of tree biodiversity in Los Angeles, CA. Landsc Urban Plan. 116:48–59. doi:10.1016/j.landurbplan.2013.04.006.
  • Cribari-Neto F, Zeileis A. 2010. Beta regression in R. J Stat Softw. 34(2):1–24. doi:10.18637/jss.v034.i02.
  • Daily GC. 1997. Nature’s services: societal dependence on natural ecosystems. Washington (DC): Island Press.
  • Dales RE, Cakmak S, Judek S, Coates F. 2008. Tree pollen and hospitalization for asthma in urban Canada. Int Arch Allergy Immunol. 146(3):241–247. doi:10.1159/000116360.
  • Dobbs C, Kendal D, Nitschke CR. 2014. Multiple ecosystem services and disservices of the urban forest establishing their connections with landscape structure and sociodemographics. Ecol Indic. 43:44–55. doi:10.1016/j.ecolind.2014.02.007.
  • Doick KJ, Peace A, Hutchings TR. 2014. The role of one large greenspace in mitigating London’s nocturnal urban heat island. Sci Total Environ. 493:662–671. 10.1016/j.scitotenv.2014.06.048.
  • Dunn OJ. 1961. Multiple comparisons among means. J Am Stat Assoc. 56(293):52. doi:10.2307/2282330.
  • Dyment JE, Bell AC. 2008. Grounds for movement: green school grounds as sites for promoting physical activity. Health Educ Res. 23(6):952–962. doi:10.1093/her/cym059.
  • Dzierzanowski K, Popek R, Gawronska H, Saebo A, Gawronski SW. 2011. Deposition of particulate matter of different size fraction on leaf surfaces and in waxes of urban forests species. Int J Phytorem. 13(10):1037–1046. doi:10.1080/15226514.2011.552929.
  • Ellaway A, Morris G, Curtice J, Robertson C, Allardice G, Robertson R. 2009. Associations between health and different types of environmental incivility: a Scotland-wide study. Public Health. 123(11):708–713. doi:10.1016/j.puhe.2009.09.019.
  • Erkip F. 1997. The distribution of urban public services: the case of parks and recreational services in Ankara. Cities. 14(6):353–361. doi:10.1016/S0264-2751(97)00026-7.
  • Ferrari SLP, Cribari-Neto F. 2004. Beta regression for modelling rates and proportions. J Appl Stat. 31(7):799–815. doi:10.1080/0266476042000214501.
  • Fjørtoft I. 2004. Landscape as playscape: the effects of natural environments on children’s play and motor development. Child Youth Environ. 14(2):21–44. https://www.jstor.org/stable/10.7721/chilyoutenvi.14.2.0021.
  • Florvaag E, Elsayed S, Apold J. 1982. Comparative studies on tree pollen allergens: II. isolation of alder (alnus incana) pollen allergens: purification and some characteristics of the major allergen pi 4.78. Int Arch Allergy Immunol. 67:49–56. doi:10.1159/000232987.
  • Franchi GG, Nepi M, Matthews ML, Pacini E. 2007. Anther opening, pollen biology and stigma receptivity in the long blooming species, Parietaria judaica L. (Urticaceae). Flora: Morphol, Distrib, Funct Ecol Plants. 202(2):118–127. doi:10.1016/j.flora.2006.03.005.
  • Friedman J 2009. The ecology and evolution of wind pollination. PhD. thesis, University of Toronto, Toronto (Ontario, Canada). 250 p.
  • García-Mozo H, Oteros JA, Galán C. 2016. Impact of land cover changes and climate on the main airborne pollen types in Southern Spain. Sci Total Environ. 548–549:221–228. doi:10.1016/j.scitotenv.2016.01.005.
  • Grahn P, Stigsdotter UA. 2003. Landscape planning and stress. Urban For Urban Greening. 2:1–18. doi:10.1078/1618-8667-00019.
  • Heidt V, Neef M. 2008. Benefits of urban green space for improving urban climate. In: Carreiro MM, Song YC, Wu J, editors. Ecology, planning, and management of urban forests. New York (NY): Springer; p. 84–96.
  • Herrington S, Studtmann K. 1998. Landscape interventions: new directions for the design of children’s outdoor play environments. Landsc Urban Plan. 42(2–4):191–205. doi:10.1016/S0169-2046(98)00087-5.
  • Heynen NC, Lindsey G. 2003. Correlates of urban forest canopy cover: implications for local public works. Public Works Manage Policy. 8(1):33–47. doi:10.1177/1087724X03008001004.
  • Hope D, Gries C, Zhu W, Fagan WF, Redman CL, Grimm NB, Nelson AL, Martin C, Kinzig A. 2003. Socioeconomics drive urban plant diversity. Proc Natl Acad Sci U S A. 100(15):8788–8792. doi:10.1073/pnas.1537557100.
  • Idilfitri S, Sulaiman S, Salleh NS. 2014. Role of ornamental plants for bird community’ habitats in urban parks. Procedia – Social Behav Sci. 153:666–677. doi:10.1016/j.sbspro.2014.10.098.
  • Kabisch N, Haase D, Van den Bosch MA. 2016. Adding natural areas to social indicators of intra-urban health inequalities among children: a case study from Berlin, Germany. Int J Environ Res Public Health. 13(8):783. doi:10.3390/ijerph13080783.
  • Kearns A, Whitley E, Bond L, Tannahill C. 2012. The residential psychosocial environment and mental wellbeing in deprived areas. Int J Hous Policy. 12(4):413–438. doi:10.1080/14616718.2012.711985.
  • Kleinman M, Geiger JR. 2002. Trees in our city. Davis (CA): Center for Urban Forest Research, Pacific Southwest Research Station, USDA Forest Service, ppt.; p. 13.
  • Landry SM, Chakraborty J. 2009. Street trees and equity: evaluating the spatial distribution of an urban amenity. Environ Plan A. 41(11):2651–2670. doi:10.1068/a41236.
  • Latinopoulos D, Mallios Z, Latinopoulos P. 2016. Valuing the benefits of an urban park project: a contingent valuation study in Thessaloniki, Greece. Land Use Policy. 55:130–141. doi:10.1016/j.landusepol.2016.03.020.
  • Latorre F. 1999. Differences between airborne pollen and flowering phenology of urban trees with reference to production, dispersal and interannual climate variability. Aerobiologia. 15(2):131–141. doi:10.1023/A:1007523316024.
  • Lemon J. 2006. Plotrix: a package in the red light district of R. R-News. R Package Version 3 5-11. 6(4):8–12. http://CRAN.R-project.org/package=plotrix.
  • Leong M, Dunn RR, Trautwein MD. 2018. Biodiversity and socioeconomics in the city: a review of the luxury effect. Biol Letters, Royal Soc Publ. 14:5. doi:10.1098/rsbl.2018.0082.
  • Lin RY, Clauss AE, Bennett ES. 2002. Hypersensitivity to common tree pollens in New York city patients. Allergy Asthma Proc. 23(4):253–258. doi:10.1016/S0091-6749(02)81434-8.
  • Liu H, Hu Y, Li F, Yuan L. 2018. Associations of multiple ecosystem services and disservices of urban park ecological infrastructure and the linkages with socioeconomic factors. J Clean Prod. 174:868–879. doi:10.1016/j.jclepro.2017.10.139.
  • Livesley SJ, McPherson EG, Calfapietra C. 2016. The urban forest and ecosystem services: impacts on urban water, heat, and pollution cycles at the tree, street, and city scale. J Environ Qual. 45(1):119–124. doi:10.2134/jeq2015.11.0567.
  • Lõhmus M, Balbus J. 2015. Making green infrastructure healthier infrastructure. Infect Ecol Epidemiol. 5(1):30082. doi:10.3402/iee.v5.30082.
  • Lovasi GS, O’Neil-Dunne JPM, Lu JWT, Sheehan D, Perzanowski MS, MacFaden SW, King KL, Matte T, Miller RL, Hoepner LA, et al. 2013. Urban tree canopy and asthma, wheeze, rhinitis, and allergic sensitization to tree pollen in a New York city birth cohort. Environ Health Perspect. 121(4):494–500. doi:10.1289/ehp.1205513.
  • Lyytimäki J, Sipilä M. 2009. Hopping on one leg - the challenge of ecosystem disservices for urban green management. Urban For Urban Greening. 8(4):309–315. doi:10.1016/j.ufug.2009.09.003.
  • Mallen E, Bakin J, Stone B, Sivakumar R, Lanza K. 2020. Thermal impacts of built and vegetated environments on local microclimates in an urban university campus. Urban Clim. 32:100640. doi:10.1016/j.uclim.2020.100640.
  • Martin CA, Warren PS, Kinzig AP. 2004. Neighbourhood socioeconomic status is a useful predictor of perennial landscape vegetation in residential neighbourhoods and embedded small parks of Phoenix, AZ. Landsc Urban Plan. 69(4):355–368. doi:10.1016/j.landurbplan.2003.10.034.
  • May KL. 2000. Sensitivity to birch pollen–under-appreciated etiology of atopic asthma in towns. Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego. Polskiego Towarzystwa Alergologicznego, I Instytutu Gruzlicy I Chorób Pluc. 68(9–10):478–485.
  • McConnachie M, Shackleton CM. 2010. Public green space inequality in small towns in South Africa. Habitat Int. 34(2):244–248. doi:10.1016/j.habitatint.2009.09.009.
  • Mcdonald JE. 1962. Collection and washout of airborne pollens and spores by raindrops. Sci. 135(3502):435–437. doi:10.1126/science.135.3502.435.
  • McInnes RN, Hemming D, Burgess P, Lyndsay D, Osborne NJ, Skjøth CA, Thomas S, Vardoulakis S. 2017. Mapping allergenic pollen vegetation in UK to study environmental exposure and human health. Sci Total Environ. 599–600:483–499. doi:10.1016/j.scitotenv.2017.04.136.
  • McPherson EG, Van Doorn NS, Peper PJ 2016. Urban tree database and allometric equations. U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station; p. 86. General Technical Report PSW-GTR-253.
  • Melles SJ. 2005. Urban bird diversity as an indicator of human social diversity and economic inequality in Vancouver, British Columbia. Urban Habitats. 3(1):25–48. http://urbanhabitats.org/v03n01/vancouver_full.html.
  • Molina RT, López FG, Rodríguez AM, Palaciso IS. 1996. Pollen production in anemophilous trees. Grana. 35(1):38–46. doi:10.1080/00173139609430499.
  • Myrup LO. 1969. A numerical model of the urban heat island. J Appl Meteorol. 8:908–918. doi:10.1175/1520-0450(1969)008<0908:ANMOTU>2.0.CO;2.
  • Nesbitt L, Meitner MJ. 2016. Exploring relationships between socioeconomic background and urban greenery in Portland, OR. Forests. 7(8):1–14. doi:10.3390/f7080162.
  • Neyman J, Pearson ES. 1967. Joint statistical papers. London:Cambridge University Press.
  • Nguyen N-A, Eskelson BNI, Gergel SE, Murray T. 2021. The occurrence of invasive plant species differed significantly across three urban greenspace types of Metro Vancouver, Canada. Urban For Urban Greening. 59:126999.
  • Nowak DJ, Hirabayashi S, Doyle M, McGovern M, Pasher J. 2018. Air pollution removal by urban forests in Canada and its effect on air quality and human health. Urban For Urban Greening. 29:40–48. doi:10.1016/j.ufug.2017.10.019.
  • Oke TR. 1982. The energetic basis of the urban heat island. Q J Royal Meteorol Soc. 108(455):1–24. doi:10.1002/qj.49710845502.
  • Paciência I, Cavaleiro Rufo J, Ribeiro AI, Mendes FC, Farraia M, Silva D, Delgado L, Moreira A. 2020. Association between the density and type of trees around urban schools and exhaled nitric oxide levels in schoolchildren. Eur Ann Allergy Clin Immunol. doi:10.23822/eurannaci.1764-1489.162.
  • Paciência I, Rufo JC, Silva D, Martins C, Mendes F, Rama T, Rodolfo A, Madureira J, Delgado L, De Oliveira Fernandes E, et al. 2019. School environment associates with lung function and autonomic nervous system activity in children: a cross-sectional study. Sci Rep. 9:15156. doi:10.1038/s41598-019-51659-y.
  • Park BJ, Furuya K, Kasetani T, Takayama N, Kagawa T, Miyazaki Y. 2011. Relationship between psychological responses and physical environments in forest settings. Landsc Urban Plan. 102(1):24–32. doi:10.1016/j.landurbplan.2011.03.005.
  • Poudyal NC, Hodges DG, Merrett CD. 2009. A hedonic analysis of the demand for and benefits of urban recreation parks. Land Use Policy. 26(4):975–983. doi:10.1016/j.landusepol.2008.11.008.
  • Pretty J, Peacock J, Sellens M, Griffin M. 2005. The mental and physical health outcomes of green exercise. Int J Environ Health Res. 15(5):319–337. doi:10.1080/09603120500155963.
  • R Core Team. 2019. R: a language and environment for statistical computing. Vienna (Austria): R Foundation for Statistical Computing. https://www.R-project.org/.
  • Räsänen L, Reunala T, Lehto M, Virtanen E, Arvilommi H. 1992. Immediate and delayed hypersensitivity reactions to birch pollen in patients with atopic dermatitis. Acta Derm Venereol. 72(3):193–196. doi:10.102340/0001555572193196.
  • Reed SD, Lee TA, McCrory DC. 2004. The economic burden of allergic rhinitis: a critical evaluation of the literature. PharmacoEconomics. 22:345–361. doi:10.2165/00019053-200422060-00002.
  • Rojo J, Rapp A, Lara B, Fernández-González F, Pérez-Badia R. 2015. Effect of land uses and wind direction on the contribution of local sources to airborne pollen. Sci Total Environ. 538:672–682. doi:10.1016/j.scitotenv.2015.08.074.
  • Ruan X, Huang J, Williams DAR, Harker KJ, Gergel SE. 2019. High spatial resolution landscape indicators show promise in explaining water quality in urban streams. Ecol Indic. 103:321–330. doi:10.1016/j.ecolind.2019.03.013.
  • Sanesi G, Lafortezza R, Bonnes M, Carrus G. 2006. Comparison of two different approaches for assessing the psychological and social dimension of green spaces. Urban For Urban Greening. 5:121–129. doi:10.1016/j.ufug.2006.06.001.
  • Schwartz J, Weiss ST. 1995. Relationship of skin test reactivity to decrements in pulmonary function in children with asthma or frequent wheezing. Am J Respir Crit Care Med. 152(6pt. I):2176–2180. doi:10.1164/ajrccm.152.6.8520794.
  • SimplyAnalytics. 2018. Census 2018 median annual household income. SimplyAnalytics database. [accessed 2019 May 28].
  • Sivarajah S, Smith SM, Thomas SC, Reddy SV. 2018. Tree cover and species composition effects on academic performance of primary school students. PLoS ONE. 13:2. doi:10.1371/journal.pone.0193254.
  • Skjøth CA, Sikoparija B, Jager S. 2012. Pollen sources. In: Sofiev M, Bergmann K-C, editors. Allergenic pollen: a review of the production, release, distribution and health impacts. New York (NY): Springer; p. 9–27.
  • Skjøth CA, Ørby PV, Becker T, Geels C, Schlünssen V, Sigsgaard T, Bønløkke JH, Sommer J, Søgaard P, Hertel O, et al. 2013. Identifying urban sources as cause of elevated grass pollen concentrations using GIS and remote sensing. Biogeosciences. 10(1):541–554. doi:10.5194/bg-10-541-2013.
  • Statistics Canada. 2016. Median household total income in 2015, by 2016 census division (CD). Census of Population. https://www12.statcan.gc.ca/census-recensement/2016/geo/map-carte/ref/thematic-thematiques/inc-rev/map-eng.cfm?TYPE=1.
  • Takaro TK, Knowlton K, Balmes JR. 2013. Climate change and respiratory health: current evidence and knowledge gaps. Expert Rev Respir Med. 7(4):349–361. doi:10.1586/17476348.2013.814367.
  • Taylor AF, Kuo FE, Sullivan WC. 2001. Coping with ADD. The surprising connection to green play settings. Environ Behav. 33(1):54–77. doi:10.1177/00139160121972864.
  • Thompson JL, Thompson JE. 2003. The urban jungle and allergy. Immunol Allergy Clin North Am. 23(3):371–387. doi:10.1016/S0889-8561(03)00006-7.
  • Thompson RS. 1993. Building amplification factors for sources near buildings: a wind-tunnel study. Atmos Environ Part A, Gener Topics. 27(15):2313–2325. doi:10.1016/0960-1686(93)90400-S.
  • Tranter PJ, Malone K. 2004. Geographies of environmental learning: an exploration of children’s use of school grounds. Children’s Geographies. 2(1):131–155. doi:10.1080/1473328032000168813.
  • Vancouver Board of Parks and Education. 2018a. VanPlay: inventory and analysis. Chapter 2, Vancouver, British Columbia, Canada: Vancouver Board of Parks and Recreation; p. 29.
  • Vancouver Board of Parks and Education. 2018b. VanPlay Report 2: 10 Goals to Shape the Next 25 Years. p. 25.
  • Vilhar U. 2017. Water regulation and purification. In: Pearlmutter D, Calfapietra C, Samson R, O’Brien L, Krajter Ostoic S, Sanesi G, Alonso del Amo R, editors. The urban forest: cultivating green infrastructure for people and the environment. Vol. 7. Future City: Springer International Publishing; p. 41–47.
  • Villeneuve PJ, Jerrett M, Su J G, Burnett RT, Chen H, Wheeler AJ, Goldberg MS. 2012. A cohort study relating urban green space with mortality in Ontario, Canada. Environ Res. 115:51–58. doi:10.1016/j.envres.2012.03.003.
  • Von Döhren P, Haase D. 2015. Ecosystem disservices research: a review of the state of the art with a focus on cities. Ecol Indic. 52:490–497. doi:10.1016/j.ecolind.2014.12.027.
  • Wang HF, Qureshi S, Knapp S, Friedman CR, Hubacek K. 2015. A basic assessment of residential plant diversity and its ecosystem services and disservices in Beijing, China. Appl Geogr. 64:121–131. doi:10.1016/j.apgeog.2015.08.006.
  • Watkins SL, Mincey SK, Vogt J, Sweeney SP. 2017. Is planting equitable? An examination of the spatial distribution of nonprofit urban tree-planting programs by canopy cover, income, race, and ethnicity. Environ Behav. 49(4):452–482. doi:10.1177/0013916516636423.
  • Weiss CC, Purciel M, Bader M, Quinn JW, Lovasi G, Neckerman KM, Rundle AG. 2011. Reconsidering access: park facilities and neighbourhood disamenities in New York city. J Urban Health. 88(2):297–310. doi:10.1007/s11524-011-9551-z.
  • WHO Regional Office for Europe. 2016. Urban Green Spaces and Health. p. 25.
  • Williams DAR, Matasci G, Coops NC, Gergel SE. 2018. Object-based urban landcover mapping methodology using high spatial resolution imagery and airborne laser scanning. J Appl Remote Sens. 12(4):1. doi:10.1117/1.jrs.12.046020.
  • Yang L, Zhang L, Li Y, Wu S. 2015. Water-related ecosystem services provided by urban green space: a case study in Yixing city (China). Landsc Urban Plan. 136:40–51. doi:10.1016/j.landurbplan.2014.11.016.
  • Zhou X, Parves Rana M. 2012. Social benefits of urban green space: a conceptual framework of valuation and accessibility measurements. Manage Environ Quality. 23(2):173–189. doi:10.1108/14777831211204921.
  • Ziter CD, Pedersen EJ, Kucharik CJ, Turner MG. 2019. Scale-dependent interactions between tree canopy cover and impervious surfaces reduce daytime urban heat during summer. Proc Natl Acad Sci U S A. 116(15):7575–7580. doi:10.1073/pnas.1817561116.