58
Views
9
CrossRef citations to date
0
Altmetric
(RG) Obstetrics and Gynaecology

Flow cytometry in human reproductive biology

, , , &
Pages 505-521 | Published online: 07 Jul 2009

References

  • Melamed MR, Lindmo T, Mendelsohn ML, eds. Flow Cytometry and Cell Sorting, 2nd edn. New York: Wiley-Liss, 1990
  • Meseguer M, Aplin JD, Caballero-Campo P, et al. Human endometrial MUC1 is up-regulated by progesterone and down-regulated in vitro by the human blastocyst. Biol Reprod 2001;64: 590–601
  • Garrido N, Albert C, Krussel JS, et al. No differences in expression, production and secretion of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) by granulosa cells in women with endometriosis. Fertil Steril 2001;76:568–75
  • Lachapelle MH, Hemmings R, Roy DC. Flow cytometric evaluation of leukocyte subpopulations in the follicular fluids ofinfertile patients. Fertil Steril 1996;65:1135–40
  • Toya M, Saito H, Ohta N, et al. Moderate and severe endometriosis is associated with alterations in the cell cycle of granulosa cells in patients under-going in vitro fertilization and embryo transfer. Fertil Steril 2000;73:344–50
  • Garrido N, Galin A, O'ConnorJE, et al. Leukocyte subpopulations in ovulatory follicles in patients with endometriosis. Cytometry 2000;10(Supple-ment 10):85
  • Simon C, Gimeno MJ, Mercader A, et al. Embryonic regulation of integrins b3, a4 and al in human endometrial epithelial cells in vitro. J Clin Endocrinol Metab 1997;82:2607–16
  • Galan A, O'Connor JE, Valbuena D, et al. The human blastocyst regulates endometrial epithelial apoptosis in embryonic adhesion. Biol Reprod 2000; 63:430–9
  • Cabarello-Campo P, Coloma J, Meseguer M, et al. Hormonal and embryonic regulation of chemokines IL-8, MCP-1 and RANTES in the human endometrium. Mol Hum Reprod 2002;in press
  • Ferrara F, Daverio R, Mazzini G. Automation of human sperm cell analysis by flow cytometry. Clin Chem 1997;43:801–7
  • Hacker-Klom UB, Gödhe W, Nieschlag E, et al. DNA flow cytometry of human semen. Hum Reprod 1999;14:2506–12
  • Cooper TG, Atkinson AD, Nieschlag E. Experi-ence with external quality control in spermatology. Hum Reprod 1999;14:765–9
  • Gledhill BL, Evenson DP, Pinkel D. Flow cyto-metry and sorting of male germ cells. In Melamed MR, Lindmo T, Mendelsohn ML, eds. Flow Cytometry and Cell Sorting, 2nd edn. New York: Wiley-Liss, 1990:531–51
  • Garrido N, Navarro J, Remohi J, et al. Follicular hormonal environment and embryo quality in women with endometriosis. Hum Reprod Updates 2000;6:67–74
  • Ntrivalas El, Kwak-Kim JY, Gilman-Sachs A, et al. Status of peripheral blood natural killer cells in women with recurrent spontaneous abortions and infertility of unknown aetiology. Hum Reprod 2001;16:855–61
  • Garrido N, Albert C, O'Connor JE, et al. The role of leukocytes in the secretion of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) of women at risk of ovarian hyper-stimulation syndrome (OHSS).J Soc Gynecol Invest 1999;6\(Supplement 1);141 (abstract)
  • Meseguer M, Pellicer A, Simon C. MUC1 and endometrial receptivity. Mol Hum Reprod 1998; 4:1089–98
  • Simon C, Martin JC, Meseguer M, et al. Embryonic regulation of endometrial molecules in human implantation. J Reprod Fertil 2000;55 (Supplement):43–53
  • Xing P-X, Reynolds K, Tjandra JJ, et al. Synthetic peptides reactive with anti-human milk fat globule membrane monoclonal antibodies. Cancer Res 1990;50:89–96
  • Gonzalez RR, Palomino A, Boric A, et al. A quan-titative evaluation of alpha1, alpha4, alphaV and beta3 endometrial integrins of fertile and un-explained infertile women during the menstrual cycle. A flow cytometric appraisal. Hum Reprod 1999;14:2485–92
  • Demir Weusten AY, Groothuis PG, Dunselman GA, et al. Morphological changes in mesothelial cells induced by shed menstrual endometrium in vitro are not primarily due to apoptosis or necrosis. Hum Reprod 2000;15:1462–8
  • Grunwald K, Feldmann K, Melsheimer P, et al. Aneuploidy in human granulosa lutein cells obtained from gonadotrophin-stimulated follicles and its relation to intrafollicular hormone concen-trations. Hum Reprod 1998;13:2679–87
  • Pemberton L, Taylor-Papadimitriou J, Gendler SJ. Antibodies to the cytoplasmic domain of the MUC1 mucin show conservation throughout mammals. Biochem Biophys Res Commun 1992;185: 167–75
  • Garrido N, Meseguer M, Gil-Salom M, et al. Semen and testes biopsy characterization of their cellular populations by flow cytometry DNA analysis. Presented at the 1 er Congreso de la Asociación para el Estudio de la Biologia de la Reproducción (ASEBIR) Revista Iberoamericana de Fertilidad 2001; in press
  • Kaufman DG, Nagler HM. Aspiration flow cytometry of the testes in the evaluation of spermatogenesis in the infertile male. Fertil Steril 1987;48:287–91
  • Mallidis C, Baker HW. Fine-needle aspiration biopsy of the testis. Fertil Steril 1994;61:367–75
  • Kostakopoulos A, Georgulakis J, Deliveliotis C, et al. Deoxyribonucleic acid flow cytometry in the assessment of spermatogenesis. J Urol 1997; 58:79–81
  • Mays-Hoopes LL, Bolen J, Riggs AD, et al. Prepa-ration of spermatogonia, spermatocytes and round spermatids for analysis of gene expression using fluorescence-activated cell sorting. Biol Reprod 1995;53:1003–11
  • Johnson LA, Flook JP, Hawk HW. Sex pre-selection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Biol Reprod 1989;41:199–203
  • Johnson LA. Sex preselection in swine: altered sex ratios in offipring following surgical insemination of flow sorted X- and Y-bearing sperm. Reprod Dom Anim 1991;29:309–14
  • Cran DG, Johnson LA, Miller NG, et al. Produc-tion of bovine calves following separation of X- and Y-chromosome-bearing sperm and in vitro fertilization. Vet Res 1993;132:40–1
  • Rath D, Johnson LA, Dobrinski JR. Births of piglets following in vitro fertilisation using sperm flow cytometrically sorted for gender. Therio-genology 1996;45:256
  • Hamano K, Li X, Qian XQ, et al. Gender pre-selection in cattle with intracytoplasmically injected, flow cytometrically sorted sperm heads. Biol Reprod 1999;60:1194–7
  • Johnson LA, Schulman J. The safety ofsperm selec-tion by flow cytometry. Hum Reprod 1994;9:758–9
  • Pinkel D, Gledhill BL, Lake S, et al. Sex pre-selection in mammals? Separation of sperm bearing Y and '0' chromosomes in the vole (Microtus oregoni). Science 1982;218:904–6
  • Johnson LA, Pinkel D. Modification of a laser-based flow cytometer for high-resolution DNA analysis of mammalian spermatozoa. Cytometry 1986;7:268–73
  • Johnson LA, Welch GR, Keyvanfar K, et al. Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the pre-vention of X-linked diseases. Hum Reprod 1993; 8:1733–9
  • Vidal F, Fugger EF, Blanco J, et al. Efficiency of MicroSortflow cytometry for producing sperm populations enriched in X- or Y-chromosome haplotypes: a blind trial assessed by double and triple colour fluorescent in situ hybridization. Hum Reprod 1998;13:308–12
  • Levinson G, Keyvanfar K, Wu JC, et al. DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease. Hum Reprod 1995;10:979–82
  • Fugger EF, Black SH, Keyvanfar K, et al. Births of normal daughters after MicroSort sperm separation and intrauterine insemination, in vitro fertilization, or intracytoplasmic sperm injection. Hum Reprod 1998;13:2367–70
  • Johnson JL, Welch GR. Sex preselection: high-speed flow cytometric sorting o f X and Y sperm for maximum efficiency. Theriogenology 1999;52: 1323–41
  • Catt SL, Sakkas D, Bizzaro D, et al. Hoescht staining and exposure to UV laser during flow cytometric sorting does not affect the frequency of detected endogenous DNA nicks in abnormal and normal human spermatozoa. Mol Hum Reprod 1997;3:821–5
  • Watkins A, Chan PC, Kalugdan TH, et al. Analysis of the flow cytometer stain Hoescht 33342 on human spermatozoa. Mol Hum Reprod 1996;2: 709–12
  • von Benhardi R, de Ioannes AE, Blanco LP, et al. Round-headed spermatozoa: a model to study the role of acrosomes in early events of gamete inter-action. Andrologia 1990;22:12–20
  • Bourne H, Liu DY, Clarke GN, et al. Normal fertilization and embryo development by intra-cytoplasmic sperm injection of round-headed acrosomeless sperm. Fertil Steril 1995;61:536–41
  • Liu DY, Baker HWG. The proportion of human sperm with poor morphology but normal intact acrosomes detected with Pisum sativum agglutinin correlates with fertilization in vitro. Fertil Steril 1988;50:288–93
  • Nikolaeva MA, Golubeva EL, Kulakov VI, et al. Evaluation of stimulus-induced acrosome reaction by two-colour flow cytometry analysis. Mol Hum Reprod 1998;4:243–50
  • Cooper TG, Yeung CH. A flow cytometric technique using peanut agglutinin for evaluating acrosomal loss from human spermatozoa. J Androl 1998;19:542–50
  • Ying X, Okabe M, Mimura T. Selection of acrosome-reacted human spermatozoa and their fusing ability by micro-injection into the peri-vitelline space of hamster eggs. Hum Reprod 1993; 8:1074–8
  • Ohashi K, Saji F, Wakimoto A, et al. Selection of acrosome-related sperm with MH61 immuno-beads. J Androl 1994;15:78–82
  • Bronson RA, Peresleni T, Go lightly M. Progester-one promotes acrosome reaction in capacitated human spermatozoa as judged by flow cytometry and CD46 staining. Mol Hum Reprod 1999;5: 507–12
  • Rdsänen M, Lähteenmäki A, Saarikoski S, et al. Comparison of flow cytometric measurement of seminal antisperm antibodies with the mixed antiglobulin reaction and the serum tray agglutina-tion test. Fertil Steril 1994;61:143–50
  • Mutaffian H, Parinaud J. Selection and character-ization of human acrosome-reacted spermatozoa. Hum Reprod 1995;10:2948–51
  • Fenichel P, Dohr G, Grivaoux C, et al. Localization and characterization of the acrosomal antigen recognized by GB24 on human spermatozoa. Mol Reprod Dev 1990;27:173–8
  • Fenichel P, Donzeau M, Farhifar D, et al. Dynamics of human sperm acrosome reaction: relation with in vitro fertilization. Fertil Steril 1991;55:994–9
  • Rufas O, Gilman A, Fisch B, et al. Spontaneous and follicular fluid-induced acrosome reaction in sperm samples from in vitro fertilizing and non-fertilizing norrnozoospermic patients. J Assist Reprod Genet 1998;15:84–9
  • Behre HM, Nieschlag E, Meschede D. Diseases of the seminal ducts. In Behre HM, Nieschlag E, eds. Andrology. Berlin: Springer, 1997:104
  • Bandoh R, Yamano S, Kamada M, et al. Effect of sperm-immobilizing antibodies on the acrosome reaction of human spermatozoa. Fertil Steril 1992; 57:387–92
  • Haas GG, Cunningham ME. Identification of antibody-laden sperm by cytofluorometry. Fertil Steril 1984;42:606–13
  • Nicholson SC, Robinson JN, Sargent IL, et al. Detection of antisperm antibodies in seminal plasma by flow cytometry: comparison with the indirect immunobead binding test. Fertil Steril 1997;68:1114–19
  • Ke RW, Dockter ME, Majumdar G, et al. Flow cytometry provides rapid and highly accurate detection of antisperm antibodies. Fertil Steril 1995;63:902–6
  • Nikolaeva MA, Kulakov VI, Ter-Avanesov GV, et al. Detection of antisperm antibodies on the surface of living spermatozoa using flow cyto-metry: preliminary study. Fertil Steril 1993;59: 639–44
  • D'Cruz OJ, Haas GJ, de La Rocha R, et al. Occur-rence of serum antisperm antibodies in patients with cystic fibrosis. Fertil Steril 1991;56:519–27
  • Haas G, D'Cruz O, De Bault L. Comparison of the indirect immunobead, radiolabeled and immunofluorescence assays for immuno globulin G serum antibodies to human sperm. Fertil Steril 1991;55:377–88
  • Kramer RY, Garner DL, Bruns ES, et al. Compari-son of motility and flow cytometric assessments of seminal quality in fresh, 24-hour extended and cryo preserve d human sp ermato zo a. J Androl 1993; 14:374–84
  • Troiano L, Granata AR, Cossarizza A, et al. Mitochondrial membrane potential and DNA stainability in human sperm cells: a flow cytometry analysis with implications for male infertility. Exp Cell Res 1998;241:384–93
  • Evenson DP, Darzynkiewicz Z, Melamed MR. Simlutaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J Histochem Cytochem 1982;30:279–80
  • Garner DL, PinkelD, JohnsonLA, et al. Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analysis. Biol Reprod 1986;34:127–38
  • Garner DL, Johnson LA. Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 1995;53:276–84
  • Ricci G, Presani G, Guaschino S, et al. Leukocyte detection in human semen using dual flow cytometry. Hum Reprod 2000;15:1329–37
  • Aslam I, Robins A, Dowell K, et al. Isolation, purification and assessment of viability of spermatogenic cells from testicular biopsies of azoospermic men. Hum Reprod 1998;13:639–45
  • Ziyyat A, Lassalle B, Testart J, et al. Flow cytometry isolation and reverse transcriptase-polymerase chain reaction characterization of human round spermatids in infertile patients. Hum Reprod 1999; 14:379–87
  • Lassalle B, Ziyyat A, Testart J, et al. Flow cyto-metric method to isolate round spermatids from mouse testis. Hum Reprod 1999;14:388–94
  • Tesarik J, Greco E, Mendoza C. ROSI, instruc-tions for use: 1997 update. Hum Reprod 1998; 13:519–23
  • Silber SJ, Johnson L. Are spermatid injections of any clinical value? ROSNI and ROSI revisited. Hum Reprod 1998;13:509–15

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.