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PRELIMINARY COMMUNICATION

A Smart Bioconjugate of Trypsin with Alginate

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Pages 325-337 | Published online: 11 Jul 2009

References

  • Arsaratnam V., Galaev I. Y., Matiasson B. Reversibly soluble biocatalyst: optimization of trypsin coupling to Eudragit S-100 and biocatalyst activity in soluble and precipitated forms. Enzyme Microb. Technol. 2000; 27: 254–263, [CSA]
  • Blanco R. M., Guisan J. M. Stabilization of enzyme by multipoint covalent attachment to agarose- aldehydes gels: borohydride reduction of trypsin-agarose derivatives. Enzyme Microb. Technol. 1989; 11: 360–366, [CROSSREF], [CSA]
  • Cheetham P. S. J. The application of enzymes in industry. Handbook of Enzyme Biotechnology. 3rd ed., A. Wisemann. Ellis Horwood Ltd., London 1995; pp. 419–552
  • Cong L., Kaul R., Dissing U., Mattiasson B. A model study on Eudragit and polyethyleneimine as soluble carriers of α-amylase for repeated hydrolysis of starch. J. Biotechnol. 1995; 42: 75–84, [CROSSREF]
  • Cornish-Bowden A. Analysis of Enzyme Kinetic Data. Oxford University Press, Oxford 1995; 27–36
  • Dominguez E., Nilsson M., Hahn-Hagerdal B. Carbodiimide coupling of β-galactosidase from Aspergillus orygae alginate. Enzyme Microb. Technol. 1988; 10: 606–610, [CROSSREF], [CSA]
  • Fujii M., Taniguchi M. Application of reversibly soluble insoluble polymers in bioprocessing. Trends Biotechnol. 1991; 9: 191–196, [PUBMED], [INFOTRIEVE], [CROSSREF]
  • Fujimora M., Mori T., Tosa T. Preparation and properties of soluble–insoluble immobilized proteases. Biotechnol. Bioeng. 1987; 29: 747–752, [CSA]
  • Gupta M. N., Guoqiang D., Mattiasson B. Purification of endopolygalacturonase by affinity precipitation using alginate. Biotechnol. Appl. Biochem. 1993; 18: 321–327, [PUBMED], [INFOTRIEVE], [CSA]
  • Gupta M. N., Mattiasson B. Unique applications of immobilized proteins in bioanalytical systems. Bioanalytical Applications of Enzymes, C. H. Suelter. John Wiley and Sons, Inc., New York 1992; 36: 1–35
  • Hoffmann A. S. Bioconjugates of intelligent polymers and recognition properties for use in diagnostics and affinity separations. Clin. Chem. 2000; 46: 1478–1486, [CSA]
  • Ibrahim M., Hubert P., Dellacherie E. Covalent attachment of epoxide hydrolase to dextran. Enzyme Microb. Technol. 1985; 7: 66–72, [CROSSREF]
  • Lowry O. H., Rosebrough N. J., Fall A. C, Randall R. J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951; 193: 265–275, [PUBMED], [INFOTRIEVE]
  • Nguyen A. L., Luong J. H. T. Synthesis and applications of water soluble reactive polymers for purification and immobilization of biomolecules. Biotechnol. Bioeng. 1989; 34: 1186–1190
  • Petro M., Svec F., Frechet J. M. J. Immobilization of trypsin onto “molded” macroporous poly (glycidyl methacrylate-co-ethylene dimethacrylate) rods and use of the conjugates as bioreactors and for affinity chromatography. Biotechnol. Bioeng. 1996; 49: 355–363, [CSA]
  • Righetti P. G., Tudor G., Kristina E. Isoelectric points and molecular weights of proteins: a new table. J. Chromatogr. 1981; 220: 115–194, [CROSSREF]
  • Roy I., Sharma S., Gupta M. N. Smart biocatalysts: design and application. Adv. Biochem. Eng. Biotechnol. 2003, Accepted for publication
  • Sardar M., Agarwal R., Kumar A., Gupta M. N. Noncovalent immobilization of enzymes on an enteric polymer Eudragit S-100. Enzyme Microb. Technol. 1997; 20: 361–367, [CROSSREF], [CSA]
  • Sardar M., Roy I., Gupta M. N. Simultaneous purification and immobilisation of Aspergillus niger xylanase on the reversibly soluble insoluble polymer Eudragit L-100. Enzyme Microb. Technol. 2000; 27: 672–679, [PUBMED], [INFOTRIEVE], [CROSSREF], [CSA]
  • Schmid F. X. Spectral methods of characterizing protein conformation and conformational changes. Protein Structure: A Practical Approach, T. E. Creighton. IRL Press, Oxford 1985; 251–285
  • Sears P. S., Clark D. S. Comparison of soluble and immobilized trypsin kinetics: Implications for peptide synthesis. Biotechnol. Bioeng. 1993; 42: 118–124, [CSA]
  • Smidsrod C., Skjak-Braek G. Alginate as immobilization matrix for cells. Trends Biotechnol. 1990; 8: 71–78, [PUBMED], [INFOTRIEVE], [CROSSREF]
  • Stoscheck C. M. Quantitation of protein. Methods in Enzymology: Guide to Protein Purification, M. P. Deutscher. Academic Press, Inc., San Diego 1990; Vol. 182: 50–67
  • Sudhakar K., Laberge M., Tsuneshige A., Vanderkooi J. M. Zinc-substituted hemoglobins: α- and β-chain differences monitored by high resolution emission spectroscopy. Biochemistry 1998; 37: 7177–7184, [PUBMED], [INFOTRIEVE], [CROSSREF]
  • Taniguchi M., Kobayashi M., Fujii M. Production of soluble sugars from cellulose material by repeated use of a reversibly soluble autoprecipitating cellulase. Biotechnol. Bioeng. 1992; 39: 287–292, [CSA]
  • Turoverov K. K., Kuznetsova I. M. What causes the depolarisation of trypsin and trypsinogen fluorescence: intramolecular mobility or nonradiative energy transfer?. Biophys. Chem. 1986; 25: 315–323, [PUBMED], [INFOTRIEVE], [CROSSREF]
  • Vaidya S., Srivastava R., Gupta M. N. Immobilization of acid phosphatase from Arachis hypogaea on CM cellulose. Biotechnol. Bioeng. 1987; 24: 1040–1043
  • Van Leemputten E., Horisberger M. Soluble–insoluble complex of trypsin immobilized on acrolein–acrylic acid copolymer. Biotechnol. Bioeng. 1976; 28: 587–590
  • Williams R. A., Blanch H. W. Covalent immobilization of protein monolayers for biosensor applications. Biosensors Bioelectron. 1994; 9: 159–167, [CROSSREF], [CSA]
  • Xiong Y., Wang W., Lee W., Gao R., Zhang Y. Study and clinical applications of “Emergency kit for snakebites.”. Toxicon 1997; 35: 481–481, [CROSSREF]

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