Advanced search
Publication Cover
Hemoglobin
international journal for hemoglobin research
Volume 25, 2001 - Issue 3
Submit an article Journal homepage
30
Views
3
CrossRef citations to date
0
Altmetric
Original

COULD CAPILLARY ZONE ELECTROPHORESIS OF TRYPTIC PEPTIDES BE USED FOR THE CHARACTERIZATION OF HEMOGLOBIN VARIANTS?

Changying Lin Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Béatrice Gulbis Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Emile Delobbe Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Patrick Robberecht Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Frédéric Cotton Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
,
Bernard Fontaine Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
&
Françoise Vertongen Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium
 show all
Pages 259-272 | Received 18 Aug 2000, Accepted 01 Jan 2001, Published online: 07 Jul 2009

REFERENCES

  • Bunn H. F., Forget B. G. Hemoglobin: Molecular, Genetic and Clinical Aspects. W.B. Saunders Company, PhiladelphiaPA, USA 1986
  • Baudin-Chich V., Drupt F., Decotte J. C., Wajcman H. Use of HPLC in different stages of characterization of abnormal hemoglobin. Application to a case of Hemoglobin Grady (α-118[+Glu-Phe-Thr]). Ann. Biol. Clin. (Paris) 1987; 45: 515–518
  • Baudin-Chich V., Wajcman H. Extended application of RP-HPLC for characterization of human hemoglobin variants. Clin. Chim. Acta 1987; 162: 75–83
  • Baudin-Chich V., Rochette J., Wajcman H. Strategy for structural characterization of haemoglobin variants. Acta. Haematol. 1987; 78: 127–129
  • Wajcman H., Bardakdjian J., Ducrocq R. Structural characterization of abnormal hemoglobins from dried blood specimens in a neonatal screening program. Ann. Biol. Clin. (Paris) 1993; 51: 867–870
  • Ferranti P., Malorni A., Pucci P., Fanali S., Nardi A., Ossicini L. Capillary zone electrophoresis and mass spectrometry for the characterization of genetic variants of human hemoglobin. Anal. Biochem. 1991; 194: 1–8
  • Ishioka N., Iyori N., Noji J., Kurioka S. Detection of abnormal haemoglobin by capillary electrophoresis and structural identification. Biomed. Chromatogr. 1992; 6: 224–226
  • Ross G. A., Lorkin P., Perrett D. Separation and tryptic digest mapping of normal and variant haemoglobins by capillary electrophoresis. J. Chromatogr. 1993; 636: 69–79
  • Okafo G. N., Perrett D., Camilleri P. Improved resolution of tryptic digest fragments from haemoglobin variants using phytic acid in free zone capillary electrophoresis. Biomed. Chromatogr. 1994; 8: 202–204
  • Li M. X., Liu L., Wu J. T., Lubman D. M. Use of a polybrene capillary coating in capillary electrophoresis for rapid analysis of hemoglobin variants with on-line detection via an ion trap storage/reflectron time-of-flight mass spectrometer. Anal. Chem. 1997; 69: 2451–2456
  • Shelton J. B., Shelton J. R., Schroeder W. A. High performance liquid chromatographic separation of globin chains on a large-pore C4 column. J. Liq. Chromatogr. 1984; 7(10)1969–1977
  • Wajcman H., Borensztajn K., Riou J., Promé D., Hurtrel D., Bardakdjian J., Léna-Russo D., Amouroux I., Ducrocq R. Two new Gγ chain variants: Hb F-Clamart [γ17(A14)Lys → Asn] and Hb F-Ouled Rabah [γ19(B1)Asn → Lys]. Hemoglobin 2000; 24(1)45–52
  • Lin C., Cotton F., Fontaine B., Gulbis B., Janssens J., Vertongen F. Capillary zone electrophoresis: an additional technique for the identification of hemoglobin variants. Hemoglobin 1999; 23(2)97–109
  • Lin C., Gulbis B., Delobbe E., Cotton F., Vertongen F. Separation of human globin chains by micellar electrokinetic capillary chromatography. J. Chromatogr. B. Biomed. Sci. Appl. 1998; 719: 47–54
  • Capelli L., Stoyanov A. V., Wajcman H., Righetti P. G. Generation of tryptic maps of α- and β-globin chains by capillary electrophoresis in isoelectric buffers. J. Chromatogr. A 1997; 791: 313–322
  • Righetti P. G., Gelfi C., Perego M., Stoyanov A. V., Bossi A. Capillary zone electrophoresis of oligonucleotides and peptides in isoelectric buffers: theory and methodology. Electrophoresis 1997; 18: 2145–2153
  • Grossman P. D., Wilson K. J., Petrie G., Lauer H. H. Effect of buffer pH and peptide composition on the selectivity of peptide separations by capillary zone electrophoresis. Anal. Biochem. 1988; 173: 265–270

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.