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Original Articles

Subproteomics in Analytical Chemistry: Chromatographic Fractionation Techniques in the Characterization of Proteins and Peptides

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Pages 2195-2224 | Received 18 Feb 2003, Accepted 27 Mar 2003, Published online: 06 Feb 2007

References

  • Liebler , D. C. 2002 . “ Proteomics and the new biology ” . In Introduction to Proteomics: Tools for the New Biology 3 – 13 . Totowa , N.J. : Humana Press, Inc. .
  • Zhang , B. , Palcic , M. M. , Mo , H. , Goldstein , I. J. and Hindsgaul , O. 2001 . Rapid determination of the binding affinity and specificity of the mushroom Polyporus squamosus lectin using frontal affinity chromatography coupled to electrospray mass spectrometry . Glycobiology , 11 ( 2 ) : 141 – 147 .
  • Goshe , M. B. , Veenstra , T. D. , Panisko , E. A. , Conrads , T. P. , Angell , N. H. and Smith , R. D. 2002 . Phosphoprotein isotope‐coded affinity tags: application to the enrichment and identification of low abundance phosphoproteins . Anal. Chem. , 74 : 607 – 616 .
  • Sickman , A. and Meyer , H. E. 2001 . Phosphoamino acid analysis . Proteomics , 1 : 200 – 206 .
  • Jung , E. , Heller , M. , Sanchez , J. C. and Hochstrasser , D. M. 2000 . Proteomics meets cell biology: the establishment of subcellular proteomes . Electrophoresis , 21 : 3369 – 3377 .
  • Cordwell , S. J. , Nouwens , A. S. , Verrills , N. M. , Basseal , D. J. and Walsh , B. J. 2000 . Subproteomics based upon protein cellular location and relative solubilities in conjunction with composite two‐dimensional electrophoresis gels . Electrophoresis , 21 : 1094 – 1103 .
  • Gorg , A. , Obermaier , C. , Boguth , G. , Harder , A. , Scheibe , B. , Wildgruber , R. and Weiss , W. 2000 . The current state of two‐dimensional electrophoresis with immobilized pH gradients . Electrophoresis , 21 : 1037 – 1053 .
  • Petersen , A. and Mundt , C. 2001 . Investigations on the carbohydrate moieties of glycoprotein allergens . J. Chromatogr. B , 756 : 141 – 150 .
  • Davis , M. T. , Beierle , J. , Bures , E. T. , McGilney , M. D. , Mort , J. , Robinson , J. H. , Spahr , C. S. , Yu , W. , Luethy , R. and Patterson , S. D. 2001 . Automated LC‐LC‐MS‐MS platform using binary ion‐exchange and gradient reversed‐phase chromatography for improved proteomics analysis . J. Chromatogr. B , 752 : 281 – 291 .
  • Geng , M. , Ji , J. and Regnier , F. E. 2000 . Signature‐peptide approach to detecting proteins in complex mixtures . J. Chromatogr. A , 870 : 295 – 313 .
  • Wagner , K. , Racaityte , K. , Unger , K. K. , Miliotis , T. , Edholm , L. E. , Bischoff , R. and Marko‐Varga , G. 2000 . Protein mapping by two dimensional high performance liquid chromatography . J. Chromatogr. A , 893 : 293 – 305 .
  • Wang , S. and Regnier , F. E. 2001 . Proteolysis of whole cell extracts with immobilized enzyme columns as part of multidimensional chromatography . J. Chromatogr. A , 913 : 429 – 436 .
  • Wang , S. and Regnier , F. E. 2001 . Proteomics based on selecting and quantifying cysteine containing peptides by covalent chromatography . J. Chromatogr. A , 924 : 345 – 357 .
  • Wolters , D. A. , Washburn , M. P. and Yates , J. R. III. 2001 . An automated multidimensional protein identification technology for shotgun proteomics . Anal. Chem. , 73 : 5683 – 5690 .
  • Giddings , J. C. 1984 . Two‐dimensional separations concept and promise . Anal. Chem. , 56 ( 12 ) : 1258A – 1272A .
  • Smolka , M. B. , Zhou , H. , Purkayastha , S. and Aebersold , R. 2001 . Optimization of the isotope‐coded affinity tag‐labeling procedure for quantitative proteome analysis . Anal. Biochem. , 297 : 25 – 31 .
  • Gygi , S. P. , Rist , B. , Gerber , S. A. , Turecek , F. , Gelb , M. H. and Aebersold , R. 1999 . Quantitative analysis of complex protein mixtures using isotope‐coded affinity tags . Nat. Biotechnol. , 17 : 994 – 999 .
  • Riggs , L. , Sioma , C. and Regnier , F. E. 2001 . Automated signature peptide approach for proteomics . J. Chromatogr. A , 924 : 359 – 368 .
  • Goshe , M. B. , Veenstra , T. D. , Panisko , E. A. , Conrads , T. P. , Angell , N. H. and Smith , R. 2002 . Phosphoprotein isotope‐coded affinity tags: application to the enrichment and identification of low‐abundance phosphoproteins . Anal. Chem. , 74 ( 3 ) : 607 – 616 .
  • Charkraborty , A. and Regnier , F. E. 2002 . Global internal standard technology for comparative proteomics . J. Chromatogr. A , 949 : 173 – 184 .
  • Goshe , M. B. , Conrads , T. P. , Panisko , E. A. , Angell , N. H. , Veenstra , T. D. and Smith , R. D. 2001 . Phosphoprotein isotope‐coded affinity tag approach for isolation and quantitating phosphopeptides in proteome‐wide analysis . Anal. Chem. , 73 : 2578 – 2586 .
  • Yao , X. , Freas , A. , Ramirez , J. , Demirev , P. A. and Fenselau , C. 2001 . Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus . Anal. Chem. , 73 ( 13 ) : 2836 – 2842 .
  • Ueda , E. K.M. , Gout , P. W. and Morganti , L. 2001 . Ni(II)‐based immobilized metal ion affinity chromatography of recombinant human prolactin from periplasmic Escherichia coli extracts . J. Chromatogr. A , 922 : 165 – 175 .
  • Sumi , S. , Arai , K. and Yoshida , K. I. 2001 . Separation methods applicable to prostate cancer diagnosis and monitoring therapy . J. Chromatogr. B , 764 : 445 – 455 .
  • Li , Y. , Larsson , E. L. , Jungvid , H. , Galaev , I. Y. and Mattiasson , B. 2000 . Shielding of protein‐boronate interactions during boronate chromatography of neoglycoproteins . J. Chromatogr. A , 909 : 137 – 145 .
  • Canarelli , S. , Fisch , I. and Freitag , R. 2002 . On‐line microdialysis of proteins with high‐salt buffers for direct coupling of electrospray ionization mass spectrometry and liquid chromatography . J. Chromatogr. A , 948 : 139 – 149 .
  • Janson , J. C. and Ryden , L. 1998 . Protein Purification: Principles, High‐Resolution Methods, and Applications , 2nd New York , NY : John Wiley & Sons, Inc. .
  • Machold , C. , Deinhofer , K. , Hahn , R. and Jungbauer , A. 2002 . Hydrophobic interaction chromatography of proteins I. Comparison of selectivity . J. Chromatogr. A , 972 : 3 – 19 .
  • Kato , Y. , Nakamura , K. , Kitamura , T. , Moriyama , H. , Hasegawa , M. and Sasaki , H. 2002 . Separation of proteins by hydrophobic interaction chromatography at low salt concentration . J. Chromatogr. A , 971 : 143 – 149 .
  • Ghosh , R. 2001 . Separation of proteins using hydrophobic interaction membrane chromatography . J. Chromatogr. A , 923 : 59 – 64 .
  • Hahn , R. , Deinhofer , K. , Machold , C. and Jungbauer , A. 2003 . Hydrophobic interaction chromatography of proteins II. Binding capacity, recovery and mass transfer properties . J. Chromatogr. A , 790 ( 1–2 ) : 99 – 114 .
  • Kato , Y. , Nakatani , S. , Nakamura , K. , Kitamura , T. , Moriyama , H. , Hasegawa , M. and Sasaki , H. 2003 . Hydrophobicity gradient columns for the separation of trypsin inhibitor by hydrophobic interaction chromatography at low salt concentration . J. Chromatogr. A , 986 ( 1 ) : 83 – 88 .
  • Snyder , L. R. , Kirkland , J. J. and Glajch , J. L. 1997 . Practical HPLC Method Development , 2nd New York , NY : John Wiley & Sons, Inc. .
  • Miliotis , T. , Ericsson , P. O. , Marko‐Varga , G. , Svensson , R. , Nilsson , J. , Laurell , T. and Bischoff , R. 2001 . Analysis of regulatory phosphorylation sites in ZAP‐70 by capillary high‐performance liquid chromatography coupled to electrospray ionization or matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry . J. Chromatogr. B , 752 : 323 – 334 .
  • Cao , P. and Stults , J. T. 1999 . Phosphopeptide analysis by online immobilized metal ion affinity chromatography‐capillary electrophoresis‐electrospray ionization mass spectrometry . J. Chromatogr. A , 853 : 225 – 235 .
  • Vissers , J. P.C. 1999 . Recent developments in microcolumn liquid chromatography . J. Chromatogr. A , 856 : 117 – 143 .
  • Geng , M. , Zhang , X. , Bina , M. and Regnier , F. 2001 . Proteomics of glycoproteins based on affinity selection of glycopeptides from triptic digests . J. Chromatogr. B , 752 : 293 – 306 .
  • Junyan , J. , Chakraborty , A. , Geng , M. , Zhang , X. , Amini , A. , Bina , M. and Regnier , F. 2000 . Strategy for qualitative and quantitative analysis in proteomics based on signature peptides . J. Chromatogr. B , 745 : 197 – 210 .
  • Cochet , S. , Volet , G. , Cartron , J. P. and Bertrand , O. 2001 . New procedures for glycophorin A purification with high yield and high purity . J. Chromatogr. B , 750 : 109 – 119 .
  • Liu , H. , Berger , S. J. , Chakraborty , A. B. , Plumb , R. S. and Cohen , S. A. 2002 . Multidimensional chromatography coupled to electrospray ionization time‐of‐flight mass spectrometry as an alternative to two dimensional gels for the identification and analysis of complex mixtures of intact proteins . J. Chromatogr. B , 782 : 267 – 289 .
  • Garcia , M. C. , Hogenboom , A. C. , Zappey , H. and Irth , H. 2002 . Effect of mobile phase composition of the separation and detection of intact proteins by reversed‐phase liquid chromatography‐electrospray mass spectrometry . J. Chromatogr. A , 957 : 187 – 199 .
  • Scopes , R. K. 1994 . Protein Purification: Principles and Practice , 3rd New York , N.Y. : Springer‐Verlag .
  • Sluyterman , L. A. and Elgersma , O. 1978 . Chromatofocusing: isoelectric focusing on ion‐exchange columns . J. Chromatogr. , 150 : 17 – 30 .
  • Keenan , M. J. and Holmes , R. P. 1985 . Chromatofocusing in the purification and separation of apo‐ and holo‐(vitamin D‐binding protein) . Biochem. J. , 229 : 669 – 674 .
  • Hsieh , Y. L.F. , Wang , H. , Elicone , C. , Mark , J. , Martin , S. A. and Regnier , F. 1996 . Automated analytical system for the examination of protein primary structure . Anal. Chem. , 68 : 455 – 462 .
  • Link , A. J. , Eng , J. , Schieltz , D. M. , Carmack , E. , Mize , G. J. , Morris , D. R. , Garvik , B. M. and Yates , J. R. III. 1999 . Direct analysis of protein complexes using mass spectrometry . Nat. Biotechnol. , 17 : 676 – 682 .
  • Frantzen , F. 1997 . Chromatographic and electrophoretic methods for the modified hemoglobins . J. Chromatogr. B , 699 : 269 – 286 .
  • Pala , A. , Padula , F. , Barteri , M. , Benagiano , M. , Gaudiano , M. C. , Moro , M. and Benagiano , G. 1997 . Rapid purification and properties of human glycodelin (endometrial alpha2‐globulin) . J. Chromatogr. B , 704 : 25 – 34 .
  • Xu , W. and Regnier , F. E. 1999 . Electrokinetically‐driven cation exchange chromatography of proteins and its comparison with pressure‐driven high performance liquid chromatography . J. Chromatogr. A , 853 : 243 – 256 .
  • Hunter , A. K. and Carta , G. 2002 . Protein adsorption on novel acrylamido‐based polymeric ion‐exchangers I.V. Effects of protein size on adsorption capacity and rate . J. Chromatogr. A , 971 : 105 – 116 .
  • Arvidsson , P. , Plieva , F. M. , Savina , I. N. , Lozinsky , V. I. , Fexby , S. , Bulow , L. , Galaev , I. Y. and Mattiasson , B. 2002 . Chromatography of microbial cells using continuous supermacroporous affinity and ion‐exchange columns . J. Chromatogr. A , 977 : 27 – 38 .
  • Wehr , T. 2002 . Multidimensional liquid chromatography in proteomic studies . LCGC North America , 20 ( 10 ) : 954 – 962 .
  • Porath , J. , Carlsson , J. , Olsson , I. and Belfrage , G. 1975 . Metal chelate affinity chromatography, a new approach to protein fractionation . Nature , 258 : 598 – 599 .
  • Figueroa , A. , Corradini , C. , Feibush , B. and Karger , B. L. 1986 . High‐performance immobilized metal affinity chromatography of proteins on iminodiacetic acid silica‐based bonded phases . J. Chromatogr. , 371 : 335 – 352 .
  • Andersson , L. 1984 . Fractionation of human serum proteins by immobilized metal affinity chromatography . J. Chromatogr. , 315 : 167 – 174 .
  • Millot , M. C. , Herve , F. and Sebille , B. 1995 . Retention behavior of proteins on poly(vinylimidazole)‐copper(II) complexes supported on silica: application to the fractionation of desialylated human α‐acid glycoprotein variants . J. Chromatogr. B , 664 : 55 – 67 .
  • Millot , M. C. , Herve , F. , Eap , C. B. , Duche , J. C. and Tillement , J. P. 1996 . Two step chromatographic purification of human alpha‐acid glycoprotein, its application to the purification of rare phenotype samples of the protein and their study by chromatography on immobilized metal chelate affinity adsorbants . J. Chromatogr. B , 678 : 1 – 14 .
  • Herve , F. , Fouache , F. , Marche , C. and Tillement , J. P. 1997 . Abnormal microheterogeneity detected in one commercial alpha acid glycoprotein preparation using chromatography on immobilized metal affinity adsorbent and on hydroxyapatite . J. Chromatogr. B , 688 : 35 – 46 .
  • Herve , F. , Duche , J. C. and Jaurand , M. C. 1998 . Changes in expression and microheterogeneity of the genetic variants of human alpha1‐acid glycoprotein in malignant mesothelioma . J. Chromatogr. B , 715 : 111 – 123 .
  • Posewitz , M. C. and Tempst , P. 1999 . Immobilized gallium(III) affinity chromatography of phosphopeptides . Anal. Chem. , 71 : 2883 – 2892 .
  • Gelunaite , L. , Luksa , V. , Sudziuviene , O. , Bumelis , V. and Pesliakas , H. 2000 . Chelated mercury as a ligand in immobilized metal ion affinity chromatography of proteins . J. Chromatogr. A , 904 : 131 – 143 .
  • Regnier , F. E. , Riggs , L. , Zhang , R. , Xiong , L. , Liu , P. , Charkraborty , A. , Seely , E. , Sioma , C. and Thompson , R. A. 2002 . Comparative proteomics based on stable isotope labeling and affinity selection . J. Mass Spectrom. , 37 : 133 – 145 .
  • Luo , Q. , Zou , H. , Xiao , X. , Guo , Z. , Kong , L. and Mao , X. 2001 . Chromatographic separations of proteins on metal immobilized iminodiacetic acid‐bound molded monolithic rods of macroporous poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) . J. Chromatogr. A , 926 : 255 – 264 .
  • Wang , S. , Zhang , X. and Regnier , F. E. 2002 . Quantiative proteomics strategy involving the selection of peptides containing both cysteine and histidine from tryptic digests of cell lysates . J. Chromatogr. A , 949 : 153 – 162 .
  • Xiang , H. , Wynn , R. , Nguyen , L. H.T. , Ross , O. H. , Ahrens , D. P. , O'Neil , K. T. , Hollis , G. F. and Patrick , D. R. 2002 . Immobilized metal‐ion affinity chromatography of recombinant Fab protein OPG C11 in the presence of EDTA‐Mg(II) . J. Chromatogr. A , 978 : 153 – 164 .
  • Pilatte , Y. , Rostoker , G. , Violleau , K. , Basta , M. and Hammer , C. H. 1995 . Further identification of human plasma glycoproteins interacting with the galactose‐specific lectin . Jacalin J. Chromatogr. B , 668 : 1 – 11 .
  • Hortin , G. L. and Trimpe , B. L. 1990 . Lectin affinity chromatography of proteins bearing O‐linked oligosaccharides: application of Jacalin‐agarose . Anal. Biochem. , 188 : 271 – 277 .
  • Elliott , M. A. , Elliott , H. G. , Gallagher , K. , McGuire , J. , Field , M. and Smith , K. D. 1997 . Investigation into the Concanavalin A reactivity, fucosylation and oligosaccharide microheterogeneity of alpha acid glycoprotein expressed in the sera of patients with rheumatoid arthritis . J. Chromatogr. B , 688 : 229 – 237 .
  • Fu , D. and van Halbeek , H. 1992 . N‐Glycosylation site mapping of human serotransferrin by serial lectin affinity chromatography, fast atom bombardment‐mass spectometry, and 1H nuclear magnetic resonance spectroscopy . Anal. Biochem. , 206 : 53 – 63 .
  • Geng , M. , Junyan , J. and Regnier , F. E. 2000 . Signature peptide approach to detecting proteins in complex mixtures . J. Chromatogr. A , 870 : 295 – 313 .
  • Ey , P. , Prowse , S. and Jenkins , C. 1978 . Isolation of pure IgG, IgG2a and IgG2b immunoglobulins from mouse serum using protein A‐Sepharose . Immunochemistry , 15 : 429 – 436 .
  • Riggin , A. and Regnier , F. E. 1991 . Determination of antibodies to human growth hormone in serum by protein G affinity‐reversed‐phase tandem column chromatography with fluorescence detection . Anal. Chim. Acta , 249 : 185 – 193 .
  • Hamaguchi , N. , Ellington , A. and Stanton , M. 2001 . Aptamer beacons for the direct detection of proteins . Anal. Biochem. , 294 : 126 – 131 .
  • Wilson , D. S. , Keefe , A. D. and Szostak , J. W. 2001 . The use of mRNA display to select high affinity protein‐binding peptides . Proc. Natl. Acad. Sci. , 93 ( 7 ) : 3750 – 3755 .
  • Xu , W. and Ellington , A. D. 1996 . Anti‐peptide aptamers recognize amino acid sequence and bind a protein epitope . Proc. Natl. Acad. Sci. USA , 93 : 7475 – 7480 .
  • Romig , T. S. , Bell , C. and Drolet , D. W. 1999 . Aptamer affinity chromatography: combinatorial chemistry applied to protein purification . J. Chromatogr. B , 731 : 275 – 284 .
  • Hirao , I. , Madin , K. , Endo , Y. , Yokoyama , S. and Ellington , A. D. 2000 . RNA aptamers that bind to and inhibit the ribosome‐inactivating protein, pepocin . J. Biol. Chem. , 275 ( 7 ) : 4943 – 4948 .
  • Kotia , R. B. , Li , L. and McGown , L. B. 2000 . Separation of nontarget compounds by DNA aptamers . Anal. Chem. , 72 : 827 – 831 .
  • Rehder , M. A. and McGown , L. B. 2001 . Open‐tubular capillary electrochromatography of bovine beta‐lactoglobulin variants A and B using an aptamer stationary phase . Electrophoresis , 22 : 3759 – 3764 .
  • Harlow , E. and Lane , D. 1988 . Antibodies: A Laboratory Manual Cold Spring Harbor , NY : Cold Spring Harbor Laboratory .
  • Johns , M. , Rosengarten , L. , Jackson , M. and Regnier , F. 1996 . Enzyme‐linked immunosorbent assays in a chromatographic format . J. Chromatogr. A , 743 : 195 – 206 .
  • Clarke , W. , Beckwith , J. D. , Jackson , A. , Reynolds , B. , Karle , E. M. and Hage , D. S. 2000 . Antibody immobilization to high performance liquid chromatography supports. Characterization of maximum loading capacity for intact immunoglobulin G and Fab fragments . J. Chromatogr. A , 888 : 13 – 22 .
  • Hermanson , G. T. 1996 . Bioconjugate Techniques San Diego , CA : Academic Press, Inc. .
  • Clonis , Y. D. , Labrou , N. E. , Kotsira , V. Ph. , Mazitsos , C. , Melissis , S. and Gogolas , G. 2000 . Biomimetic dyes as affinity chromatography tools for enzyme purification . J. Chromatogr. A , 891 : 33 – 44 .
  • Franco‐Fraguas , L. , Pla , A. , Ferreira , F. , Massaldi , H. , Suarez , N. and Batista‐Viera , F. 2003 . Preparative purification of soybean agglutinin by affinity chromatography and its immobilization for polysaccharide isolation . J. Chromatogr. B , 790 ( 1–2 ) : 365 – 372 .
  • Li , Y. , Larsson , E. L. , Jungvid , H. , Galaev , I. Y. and Mattiasson , B. 2000 . Shielding of protein‐boronate interactions during boronate chromatography of neoglycoproteins . J. Chromatogr. A , 909 : 137 – 145 .
  • Kishino , K. and Miyazaki , K. 1997 . Separation methods for glycoprotein analysis and preparation . J. Chromatogr. B , 699 : 371 – 381 .
  • Verdoliva , A. , Basile , G. and Fassina , G. 2000 . Affinity purification of immunoglobulins from chicken egg yolk using a new sythetic ligand . J. Chromatogr. B , 749 : 233 – 242 .
  • Palombo , G. , Verdoliva , A. and Fassina , G. 1998 . Affinity purification of IgM using a novel synthetic ligand . J. Chromatogr. B , 11 : 243 – 246 .
  • Nakamura , K. , Suzuki , T. , Kamichika , T. , Hasegawa , M. , Kato , Y. , Sasaki , H. and Inouye , K. 2002 . Evaluation and applications of a new dye affinity adsorbent . J. Chromatogr. A , 972 : 21 – 25 .
  • Palanisamy , U. D. , Winzor , D. J. and Lowe , C. R. 2000 . Synthesis and evaluation of affinity adsorbents for glycoproteins: and artificial lectin . J. Chromatogr. B , 746 : 265 – 281 .
  • Li , Y. C. , Pfuller , U. , Larsson , E. L. , Jungvid , H. , Galaev , I. Y. and Mattiasson , B. 2001 . Separation of mistletoe lectin based on the degree of glycosylation using boronate affinity chromatography . J. Chromatogr. A , 925 : 115 – 121 .
  • Trubetskoy , D. O. , Zavalova , L. L. , Akopov , S. B. and Nikolaev , L. G. 2002 . Purification of proteins specifically binding human endogenous retrovirus K long terminal repeat by affinity elution chromatography . J. Chromatogr. A , 976 : 95 – 101 .
  • Hutchens , T. W. and Yip , T. T. 1993 . New desorption strategies for the mass spectrometric analysis of macromolecules . Rapid Commun. Mass Spectrom. , 7 : 576 – 580 .
  • Brockman , A. H. and Orlando , R. 1995 . Probe‐immobilized affinity chromatography/mass spectrometry . Anal. Chem. , 67 : 4581 – 4585 .
  • Eggeling , F. V. , Junker , K. , Fiedler , W. , Wollscheid , V. , Durst , M. , Claussen , U. and Ernst , G. 2001 . Mass spectrometry meets chip technology: a new proteomic tool in cancer research? . Electrophoresis , 22 : 2898 – 2902 .
  • Merchant , M. and Weinberger , S. R. 2000 . Recent advancements in surface‐enhanced laser desorption/ionization‐time of flight‐mass spectrometry . Electrophoresis , 21 : 1164 – 1167 .
  • Nadler , T. K. , Paliwal , S. K. and Regnier , F. E. 1994 . Rapid, automated, two‐dimensional high‐performance liquid chromatographic analysis of immunoglobulin G and its multimers . J. Chromatogr. A , 676 : 331 – 335 .
  • Hsieh , Y. L.F. , Wang , H. , Elicone , C. , Mark , J. , Martin , S. A. and Regnier , F. 1996 . Automated analytical system for the examination of protein primary structure . Anal. Chem. , 68 : 455 – 462 .
  • Nadler , T. , Blackburn , C. , Mark , J. , Gordon , N. , Regnier , F. E. and Vella , G. 1996 . Automated proteolytic mapping of proteins . J. Chromatogr. A , 743 : 91 – 98 .
  • Redpath , S. and Leblanc , J. “ A new technology for an old disease: using Ciphergen ProteinChip® arrays to investigate pre‐eclampsia ” . http://www.ciphergen.com/pub (accessed Dec 2002)

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