Rapid HPLC Procedure for the Quantitation of Phytochelatins in Plant Tissue Extracts

References

• Rauser , W. E. 2000 . “ The role of thiols in plants under metal stress ” . In Sulfur Nutrition and Sulfur Assimilation in Higher Plants , 1st Ed. 169 – 183 . Switzerland : Paul Haupt .
   Google Scholar
• Takagi , M. , Satofuka , H. , Amsano , S. , Mizuno , H. , Eguchi , Y. , Hirata , K. , Miyamoto , K. , Fukui , K. and Imanaka , T. 2002 . Cellular toxicity of cadmium ions and their detoxification by heavy metal‐specific plant peptides, phytochelatins, expressed in mammalian cells . J. Biochem. , 131 : 233 – 239 .
   PubMed Web of Science ®Google Scholar
• Nillson , U. , Schutz , A. , Skerfving , S. and Mattsson , S. 1995 . Cadmium in kidneys in sweden measured in vivo using x‐ray fluorescence analysis . Int. Arch. Occup. Environ. Health , 67 : 405 – 411 .
   PubMed Web of Science ®Google Scholar
• Cobbett , C. S. 2000 . Phytochelatins and their roles in heavy metal detoxification . Plant Physiol. , 123 : 825 – 832 .
   PubMed Web of Science ®Google Scholar
• Scarano , G. and Morelli , E. 2002 . Characterization of cadmium‐ and lead‐phytochelatin complexes formed in a marine microalga in response to metal exposure . Biometals , 15 : 145 – 151 .
   PubMed Web of Science ®Google Scholar
• Satofuka , H. , Fukui , T. , Takagi , M. , Atomi , H. and Imanaka , T. 2001 . Metal‐binding properties of phytochelatin‐related peptides . J. Inorg. Biochem. , 86 : 595 – 602 .
   PubMed Web of Science ®Google Scholar
• Pickering , I. J. , Prince , R. C. , George , G. N. , Rauser , W. E. , Wickramasinghe , W. A. , Watson , A. A. , Dameron , C. T. , Dance , I. G. , Fairlie , D. P. and Salt , D. E. 1999 . X‐ray absorption spectroscopy of cadmium phytochelatin and model systems . Biochim. Biophys. Acta. , 1429 : 351 – 364 .
   PubMed Web of Science ®Google Scholar
• 1991 . Preparation and Testing of Reagent Water in the Clinical laboratory , 2nd Ed. Vilanova, PA : National Committee for Laboratory Standards . NCCLS Document C3‐A2, Vol. 11, No. 13
   Google Scholar
• Lloyd‐Williams , P. , Albericio , F. and Giralt , E. 1997 . Chemical Approaches to the Synthesis of Peptides and Proteins , 1st Ed. 19 – 81 . New York : CRC Press LLC .
   Google Scholar
• Bank , R. A. , Crusius , B. A. , Zwiers , A. , Meeuwissen , S. , Arwert , F. and Pronk , J. C. 1988 . Identification of Glu > Lys substitution in the activation segment of human pepsinogen A‐3 and ‐5 allozymogens by peptide mapping using endoproteinase Lys‐C . FEBS Lett. , 238 : 105 – 108 .
   PubMed Web of Science ®Google Scholar
• Harmens , H. , Den Hartog , P. R. , Ten Bookum , W. N. and Verkleij , J. A. 1993 . Increased zinc tolerance in Silene vulgaris (moench) garcke is not due to increased production of phytochelatins . Plant Physiol. , 103 : 1305 – 1309 .
   PubMed Web of Science ®Google Scholar
• de Knecht , J. A. , van Dillen , M. , Koevoets , P. , Schat , H. , Verkleij , J. A. and Ernst , W. 1994 . Phytochelatin in cadmium‐sensitive and cadmium‐tolerant Silene vulgaris: chain length distribution and disulfide incorporation . Plant Physiol. , 104 : 255 – 261 .
   PubMed Web of Science ®Google Scholar
• Schat , H. and Kalf , M. 1992 . Are phytochelatins involved in differential metal tolerance or do they merely reflect a metal‐imposed strain? . Plant Physiol. , 99 : 1475 – 1480 .
   PubMed Web of Science ®Google Scholar
• de Knecht , J. A. , Koevoets , P. , Verkleij , J. A. and Ernst , W. 1992 . Evidence against a role for phytochelatins in naturally selected increased cadmium tolerance in Silene vulgaris (Moench) Garcke . New Phytol. , 122 : 681 – 688 .
   Web of Science ®Google Scholar
• Vogeli‐Lange , R. and Wagner , G. J. 1990 . Subcellular localization of cadmium and cadmium‐binding peptides in tabacco leaves . Plant Physiol. , 92 : 1086 – 1093 .
   PubMed Web of Science ®Google Scholar
• Scheller , H. V. , Huang , B. , Hatch , E. and Goldsbrough , P. B. 1987 . Phytochelatin synthesis and glutathione levels in response to heavy metals in tomato cells . Plant Physiol. , 85 : 1031 – 1035 .
   PubMed Web of Science ®Google Scholar