Acute Toxicity Assessment of Azadirachtin‐Based Pesticides Using Murine Hybridoma and Oyster Cells

References

• Schmutterer , H. , Ascher , K.R. S. and Rembold , H. Proceedings of the 1st International Neem Conference . 1980 , Rottach‐Egern, Germany. Natural Pesticides from the Neem Tree , Edited by: Schmutterer , H. , Ascher , K.R. S. and Rembold , H. pp. 63 – 74 . Eschborn, , Germany : GTZ .
   Google Scholar
• Jacobson , M. 1989 . Focus on Phytochemical Pesticides: the Neem Tree Edited by: Jacobson , M. Vol. I , 69 – 86 . Boca Raton, FL : CRC Press .
   Google Scholar
• Ara , I. , Siddiqui , B. S. , Faizi , S. and Siddiqui , S. 1989 . Two new terpenoids from root bark of Azadirachta indica . J. Nat. Prod. , 52 ( 8 ) : 1209 – 1213 .
   Google Scholar
• Van Der Nat , J. M. , Van Der Sluis , W. G. , De Silva , K.T. D. and Labadie , R. P. 1991 . Ethnopharmacognostical survey of Azadirachta indica . J. Ethnopharmacol. , 35 : 1 – 24 .
   PubMed Web of Science ®Google Scholar
• Williams , J. R. , Peng , C.Y. S. , Chuand , R. Y. , Doi , R. H. and Mussen , E. C. 1998 . The inhibitory effect of Azadirachtin on Bacillus subtilus, Escherichia coli, and Paenibacillus larvae, the causative agent of American foulbrood in the honeybee, Apis mellifera L. . J. Inverteb. Pathol. , 72 ( 3 ) : 252 – 257 .
   Google Scholar
• Isman , M. B. 1997, October . Neem insecticides . Pestic. Outlook , 8 ( 5 ) : 32 – 38 .
   Google Scholar
• Kraus , W. , Bokel , M. , Bruhn , A. , Cramer , R. , Klaiber , I. , Klenck , A. , Nagl , G. , Pohnl , H. , Sadlo , H. and Vogler , B. 1987 . Structure determination by NMR of azadirachtin and related compounds from Azadirachta indica . Tetrahedron , 43 ( 12 ) : 2817 – 2830 .
   Google Scholar
• Luntz , A.J. M. and Backwell , A. 1993 . Azadirachtin: an update, a review . J. Insect Physiol. , 39 ( 10 ) : 903 – 924 .
   Google Scholar
• Low‐Friedrich , I. , Von Bredow , K. and Schoeppe , W. 1991 . A cell culture assay for the detection of cardiotoxicity . J. Pharmacol. Methods , 25 ( 2 ) : 133 – 145 .
   PubMedGoogle Scholar
• Schmutterer , H. 1995 . “ Side effects on beneficial and other ecologically important non‐target organisms ” . In The Neem Tree Azadirachta Indica and Other Meliaceous Plants Edited by: Schmuttterer , H. 495 – 517 . Weinheim : VCH .
   Google Scholar
• Bertheussen , K. , Yousef , M. I. and Figenschau , Y. 1997 . A new sensitive cell culture test for the assessment of pesticide toxicity . J. Environ. Sci. Health, Part B, Pestic. Food Contam. Agric. Wastes , 32 ( 2 ) : 195 – 211 .
   PubMed Web of Science ®Google Scholar
• Goktepe , I. 1999 . “ Toxicity of neem‐based insecticides on aquatic animals and cell lines ” . In Louisiana State University Dissertation
   Google Scholar
• Plhak , L. C. and Sporns , P. 1994 . Development and production of monoclonal antibodies for the measurement of solanidine potato glycoalkaloids . Am. Potato J. , 71 : 297 – 313 .
   Google Scholar
• Mosmann , T. 1983 . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays . J. Immunol. Methods , 65 : 55 – 63 .
   PubMed Web of Science ®Google Scholar
• Buchanan , J. T. , Roppolo , G. S. , Supan , J. E. and Tiersch , T. R. 1998 . Conditioning of eastern oyster in a closed, recirculating system . J. Shellfish Res. , 17 ( 4 ) : 1183 – 1189 .
   Google Scholar
• Buchanan , J. T. , LaPeyre , J. F. , Cooper , R. K. and Tiersch , T. R. 1999 . Improved attachment and spreading in primary cell cultures of the eastern oyster, Crassostrea virginica in vitro . Cell Dev. Biol. Anim. , 35 ( 10 ) : 593 – 598 .
   PubMedGoogle Scholar
• Buttke , T. M. , McCubrey , J. A. and Owen , T. C. 1993 . Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine‐dependent cell line . J. Immunol. Methods , 157 : 233 – 240 .
   PubMed Web of Science ®Google Scholar
• USEPA . 1993 . Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms , 4th Ed. Edited by: Weber , C. I. 38 – 94 . Cincinnati, OH : U.S. Environmental Protection Agency . EPA/600/4‐90/027F
   Google Scholar
• SAS Institute Inc., SAS® . 1995 . User's Guide: Statistics , 1995 Ed. Cary, NC : SAS Institute, Inc. .
   Google Scholar
• Cohen , E. , Quistad , G. B. , Jefferies , P. R. and Casida , J. E. 1996 . Nimbolide is the principal cytotoxic component of neem‐seed insecticide preparations . Pestic. Sci. , 48 ( 2 ) : 135 – 140 .
   Google Scholar
• Juneja , S. C. , Pfeifer , T. , Williams , R. S. and Chegini , N. 1994 . Neem oil inhibits 2‐cell embryo development and trophectoderm attachment and proliferation in vitro . J. Assist. Reprod. Genet. , 11 ( 8 ) : 419 – 427 .
   PubMedGoogle Scholar
• Rembold , H. and Annadurai , R. S. 1993 . Azadirachtin inhibits proliferation of Sf9 cells in monolayer culture . Z. Naturforsch. , 48 : 495 – 499 .
   Google Scholar
• Akudugu , J. , Gade , G. and Bohm , L. 2001 . Cytotoxicity of azadirachtin A in human glioblastoma cell lines . Life Sci. , 68 : 1153 – 1160 .
   PubMed Web of Science ®Google Scholar
• Goktepe , I. and Plhak , L. C. 2002 . Comparative toxicity of two azadirachtin‐based neem pesticides to Daphnia pulex . Environ. Toxicol. Chem. , 21 ( 1 ) : 31 – 36 .
   PubMedGoogle Scholar
• Larson , R. O. 1989 . “ The commercialization of neem ” . In Focus on Phytochemical Pesticides: the Neem Tree Edited by: Jacobson , M. Vol. I , 155 – 168 . Boca Raton : CRC Press .
   Google Scholar
• Stark , J. D. 2001 . Population‐level effects of the neem insecticide, Neemix, on Daphnia pulex . J. Environ. Sci. Health, Part B, Pestic. Food Contam. Agric. Wastes , 36 ( 4 ) : 457 – 465 .
   PubMed Web of Science ®Google Scholar
• Leight , A. K. and Dolah , R.F. V. 1999 . Acute toxicity of the insecticides endosulfan, chlorpyrifos, and malathion to the epibenthic estuarine amphipod Gammarus palustris . Environ. Toxicol. Chem. , 18 ( 5 ) : 958 – 964 .
   Google Scholar
• Tomlin , C. 1994 . The Pesticide Manual, Incorparating the Agrochemicals Handbook , 10th Ed. 115 – 124 . Cambridge, , U.K. : The Royal Society of Chemistry and British Crop Protection Council .
   Google Scholar
• Stratton , G. and Corke , G. 1981 . Interaction of permethrin with Daphnia magna in the presence and absence of particulate material . Environ. Pollut. , 24 : 135 – 144 .
   Web of Science ®Google Scholar