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Research Article

NOVEL METHOD FOR THE STUDY OF RECEPTOR Ca2+ SIGNALLING EXEMPLIFIED BY THE NK1 RECEPTOR

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Pages 241-252 | Published online: 11 Nov 2002

REFERENCES

  • Holst B., Hastrup H., Raffetseder U., Martini L., Schwartz T. W. Two Active Molecular Phenotypes of the Tachykinin NK1 Receptor Revealed by G-Protein Fusions and Mutagenesis. J. Biol. Chem. 2001; 276: 19793–19799
  • Elling C. E., Nielsen S. M., Schwartz T. W. Conversion of Antagonist-Binding Site to Metal-Ion Site in the Tachykinin NK-1 Receptor. Nature 1995; 374: 74–77
  • Holst B., Zoffmann S., Elling C. E., Hjorth S. A., Schwartz T. W. Steric Hindrance Mutagenesis Versus Alanine Scan in Mapping of Ligand Binding Sites in the Tachykinin NK1 Receptor. Mol. Pharmacol. 1998; 53: 166–175
  • Schwartz T. W. Locating Ligand-Binding Sites in 7TM Receptors by Protein Engineering. Curr. Opinion Biotechnol. 1994; 5: 434–444
  • Elling C. E., Thirstrup K., Holst B., Schwartz T. W. Conversion of Agonist Site to Metal-Ion Chelator Site in the Beta(2)–Adrenergic Receptor. Proc. Natl. Acad. Sci. USA 1999; 96: 12322–12327
  • Elling C. E., Schwartz T. W. Connectivity and Orientation of the Seven Helical Bundle in the Tachykinin NK-1 Receptor Probed by Zinc Site Engineering. EMBO J. 1996; 15: 6213–6219
  • Elling C. E., Thirstrup K., Nielsen S. M., Hjorth S. A., Schwartz T. W. Engineering of Metal-Ion Sites as Distance Constraints in Structural and Functional Analysis of 7TM Receptors. Fold. Des. 1997; 2: S76–S80
  • Thirstrup K., Elling C. E., Hjorth S. A., Schwartz T. W. Construction of a High Affinity Zinc Switch in the Kappa-Opioid Receptor. J. Biol. Chem. 1996; 271: 7875–7878
  • Holst B., Elling C. E., Schwartz T. W. Partial Agonism Through a Zinc-Ion Switch Constructed Between Transmembrane Domains III and VII in the Tachykinin NK(1) Receptor. Mol. Pharmacol. 2000; 58: 263–270
  • Heding A. Intracellular Calcium Assay Preformed on NovoStar Microtiter Plate Reader, BMG Application Note 2001

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