Effects of Buffer Ions on Isopropanol‐Enhanced Plasmid Purification Using Q‐Sepharose

References

• Sambrook , J. , Russell , D. W. and Sambrook , J. 2001 . “ Plasmids and their usefulness in molecular cloning ” . In Molecular Cloning: A Laboratory Manual , 3rd Ed. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press .
   Google Scholar
• Durland , R. H. and Eastman , E. M. 1998 . Manufacturing and quality control of plasmid‐based gene expression systems . Adv. Drug Deliv. Rev. , 30 : 33 – 48 .
   PubMed Web of Science ®Google Scholar
• Chandra , G. , Patel , P. , Kost , T. A. and Gray , J. G. 1992 . Large‐scale purification of plasmid DNA by fast protein liquid chromatography using a hi‐load Q sepharose column . Anal. Biochem. , 203 : 169 – 172 .
   PubMed Web of Science ®Google Scholar
• Prazeres , D. M.F. , Schluep , T. and Cooney , C. 1998 . Preparative purification of supercoiled plasmid DNA using anion‐exchange chromatography . J. Chromatogr. A , 806 : 31 – 45 .
   PubMed Web of Science ®Google Scholar
• Levison , P. R. , Badger , S. E. , Hathi , P. , Davies , M. J. , Bruce , I. J. and Grimm , V. 1998 . New approaches to the isolation of DNA by ion‐exchange chromatography . J. Chromatogr. A , 827 : 337 – 344 .
   PubMed Web of Science ®Google Scholar
• Endres , H. N. , Johnson , J. A.C. , Ross , C. A. , Welp , J. K. and Etzel , M. R. 2003 . Evaluation of an ion‐exchange membrane for the purification of plasmid DNA . Biotech. Appl. Biochem. , 37 : 259 – 266 .
   PubMed Web of Science ®Google Scholar
• Ferreira , G. N.M. , Cabral , J. M.S. and Prazeres , D. M.F. 2000 . Anion exchange purification of plasmid DNA using expanded bed adsorption . Bioseparation , 9 : 1 – 6 .
   PubMedGoogle Scholar
• Ferreira , G. N.M. , Cabral , J. M.S. and Prazeres , D. M.F. 1999 . Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications . Biotech. Progress , 15 : 725 – 731 .
   PubMed Web of Science ®Google Scholar
• Karlsson , E. and Ryden , L. 1988 . “ Ion‐exchange chromatography ” . In Protein Purification: Principles, High‐Resolution Methods, and Applications Edited by: Janson , J.‐C. and Ryden , L. 145 – 205 . Weinheim, , Germany : Wiley‐VCH .
   Google Scholar
• Hearn , M. T. , Hodder , A. N. and Aguilar , M. I. 1988 . High‐performance liquid chromatography of amino acids, peptides and proteins. LXXXVI. The influence of different displacer salts on the retention and bandwidth properties of proteins separated by isocratic anion‐exchange chromatography . J. Chromatogr. , 443 : 97 – 118 .
   PubMed Web of Science ®Google Scholar
• Hodder , A. N. , Aguilar , M. I. and Hearn , M. T. 1989 . High‐performance liquid chromatography of amino acids, peptides and proteins. LXXXIX. The influence of different displacer salts on the retention properties of proteins separated by gradient anion‐exchange chromatography . J. Chromatogr. , 476 : 391 – 411 .
   PubMed Web of Science ®Google Scholar
• Hodder , A. N. , Aguilar , M. I. and Hearn , M. T. 1990 . High‐performance liquid chromatography of amino acids, peptides and proteins. XCVII. The influence of the gradient elution mode and displacer salt type on the retention properties of closely related protein variants separated by high‐performance anion‐exchange chromatography . J. Chromatogr. , 506 : 17 – 34 .
   PubMed Web of Science ®Google Scholar
• Hodder , A. N. , Aguilar , M. I. and Hearn , M. T. 1990 . High‐performance liquid chromatography of amino acids, peptides and proteins. XCVIII. The influence of different displacer salts on the bandwidth properties of proteins separated by gradient elution anion‐exchange chromatography . J. Chromatogr. , 512 : 41 – 56 .
   PubMed Web of Science ®Google Scholar
• Tseng , W.‐C. and Ho , F.‐L. 2003 . Enhanced purification of plasmid DNA using Q‐sepharose by modulation of alcohol concentrations . J. Chromatogr. B , 791 : 263 – 272 .
   PubMed Web of Science ®Google Scholar
• Bohlen , P. , Stein , S. , Dairmwan , W. and Udenfriend , S. 1973 . Fluorometric assay of proteins in the nanogram range . Arch. Biochem. Biophys. , 155 : 213 – 220 .
   PubMed Web of Science ®Google Scholar
• Egli , M. 2002 . DNA‐cation interactions: quo vadis? . Chem. Biol. , 9 : 277 – 286 .
   PubMed Web of Science ®Google Scholar
• Hud , N. V. and Polak , M. 2001 . DNA‐cation interactions: the major and minor grooves are flexible ionophores . Curr. Opin. Struct. Biol. , 11 : 293 – 301 .
   PubMed Web of Science ®Google Scholar
• Koltover , I. , Wagner , K. and Safinya , C. R. 2000 . DNA condensation in two dimensions . Proc. Natl Acad. Sci. USA , 97 : 14,046 – 14,051 .
   Web of Science ®Google Scholar