The Telomerase-Specific T Motif Is a Restrictive Determinant of Repetitive Reverse Transcription by Human Telomerase

REFERENCES

• Autexier, C., and N. F. Lue. 2006. The structure and function of telomerase reverse transcriptase. Annu. Rev. Biochem. 75:493–517.
   PubMed Web of Science ®Google Scholar
• Baran, N., Y. Haviv, B. Paul, and H. Manor. 2002. Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme. Nucleic Acids Res. 30:5570–5578.
   PubMedGoogle Scholar
• Beattie, T. L., W. Zhou, M. O. Robinson, and L. Harrington. 2000. Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding. Mol. Biol. Cell 11:3329–3340.
   PubMedGoogle Scholar
• Bosoy, D., Y. Peng, I. S. Mian, and N. F. Lue. 2003. Conserved N-terminal motifs of telomerase reverse transcriptase required for ribonucleoprotein assembly in vivo. J. Biol. Chem. 278:3882–3890.
   PubMedGoogle Scholar
• Bryan, T. M., K. J. Goodrich, and T. R. Cech. 2000. Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase. Mol. Cell 6:493–499.
   PubMedGoogle Scholar
• Bryan, T. M., J. M. Sperger, K. B. Chapman, and T. R. Cech. 1998. Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax. Proc. Natl. Acad. Sci. U. S. A. 95:8479–8484.
   PubMed Web of Science ®Google Scholar
• Chan, S. R., and E. H. Blackburn. 2004. Telomeres and telomerase. Philos. Trans. R. Soc. Lond. B Biol. Sci. 359:109–121.
   PubMed Web of Science ®Google Scholar
• Collins, K. 2006. The biogenesis and regulation of telomerase holoenzymes. Nat. Rev. Mol. Cell Biol. 7:484–494.
   PubMed Web of Science ®Google Scholar
• Collins, K., and L. Gandhi. 1998. The reverse transcriptase component of the Tetrahymena telomerase ribonucleoprotein complex. Proc. Natl. Acad. Sci. U. S. A. 95:8485–8490.
   PubMedGoogle Scholar
• Collins, K., and C. W. Greider. 1993. Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation. Genes Dev. 7:1364–1376.
   PubMed Web of Science ®Google Scholar
• Delany, M. E., and L. M. Daniels. 2004. The chicken telomerase reverse transcriptase (chTERT): molecular and cytogenetic characterization with a comparative analysis. Gene 339:61–69.
   PubMedGoogle Scholar
• Drosopoulos, W. C., R. Direnzo, and V. R. Prasad. 2005. Human telomerase RNA template sequence is a determinant of telomere repeat extension rate. J. Biol. Chem. 280:32801–32810.
   PubMed Web of Science ®Google Scholar
• Drosopoulos, W. C., and V. R. Prasad. 2007. The active site residue valine 867 in human telomerase reverse transcriptase influences nucleotide incorporation and fidelity. Nucleic Acids Res. 35:1155–1168.
   PubMedGoogle Scholar
• Friedman, K. L., and T. R. Cech. 1999. Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants. Genes Dev. 13:2863–2874.
   PubMedGoogle Scholar
• Gillis, A. J., A. P. Schuller, and E. Skordalakes. 2008. Structure of the Tribolium castaneum telomerase catalytic subunit TERT. Nature 455:633–637.
   PubMed Web of Science ®Google Scholar
• Greenberg, R. A., R. C. Allsopp, L. Chin, G. B. Morin, and R. A. DePinho. 1998. Expression of mouse telomerase reverse transcriptase during development, differentiation and proliferation. Oncogene 16:1723–1730.
   PubMedGoogle Scholar
• Reference deleted.
   Google Scholar
• Guo, W., M. Okamoto, Y. M. Lee, M. A. Baluda, and N. H. Park. 2001. Enhanced activity of cloned hamster TERT gene promoter in transformed cells. Biochim. Biophys. Acta 1517:398–409.
   PubMedGoogle Scholar
• Hammond, P. W., and T. R. Cech. 1997. dGTP-dependent processivity and possible template switching of euplotes telomerase. Nucleic Acids Res. 25:3698–3704.
   PubMedGoogle Scholar
• Hammond, P. W., and T. R. Cech. 1998. Euplotes telomerase: evidence for limited base-pairing during primer elongation and dGTP as an effector of translocation. Biochemistry 37:5162–5172.
   PubMedGoogle Scholar
• Hammond, P. W., T. N. Lively, and T. R. Cech. 1997. The anchor site of telomerase from Euplotes aediculatus revealed by photo-cross-linking to single- and double-stranded DNA primers. Mol. Cell. Biol. 17:296–308.
   PubMedGoogle Scholar
• Hardy, C. D., C. S. Schultz, and K. Collins. 2001. Requirements for the dGTP-dependent repeat addition processivity of recombinant Tetrahymena telomerase. J. Biol. Chem. 276:4863–4871.
   PubMedGoogle Scholar
• Hossain, S., S. Singh, and N. F. Lue. 2002. Functional analysis of the C-terminal extension of telomerase reverse transcriptase. A putative “thumb” domain. J. Biol. Chem. 277:36174–36180.
   PubMedGoogle Scholar
• Huard, S., T. J. Moriarty, and C. Autexier. 2003. The C terminus of the human telomerase reverse transcriptase is a determinant of enzyme processivity. Nucleic Acids Res. 31:4059–4070.
   PubMedGoogle Scholar
• Jacobs, S. A., E. R. Podell, and T. R. Cech. 2006. Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase. Nat. Struct. Mol. Biol. 13:218–225.
   PubMedGoogle Scholar
• Karamysheva, Z., L. Wang, T. Shrode, J. Bednenko, L. A. Hurley, and D. E. Shippen. 2003. Developmentally programmed gene elimination in Euplotes crassus facilitates a switch in the telomerase catalytic subunit. Cell 113:565–576.
   PubMedGoogle Scholar
• Katinka, M. D., S. Duprat, E. Cornillot, G. Metenier, F. Thomarat, G. Prensier, V. Barbe, E. Peyretaillade, P. Brottier, P. Wincker, F. Delbac, H. El Alaoui, P. Peyret, W. Saurin, M. Gouy, J. Weissenbach, and C. P. Vivares. 2001. Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi. Nature 414:450–453.
   PubMed Web of Science ®Google Scholar
• Kuramoto, M., K. Ohsumi, T. Kishimoto, and F. Ishikawa. 2001. Identification and analyses of the Xenopus TERT gene that encodes the catalytic subunit of telomerase. Gene 277:101–110.
   PubMedGoogle Scholar
• Lai, C. K., M. C. Miller, and K. Collins. 2002. Template boundary definition in Tetrahymena telomerase. Genes Dev. 16:415–420.
   PubMed Web of Science ®Google Scholar
• Lai, C. K., J. R. Mitchell, and K. Collins. 2001. RNA binding domain of telomerase reverse transcriptase. Mol. Cell. Biol. 21:990–1000.
   PubMed Web of Science ®Google Scholar
• Lee, M. S., and E. H. Blackburn. 1993. Sequence-specific DNA primer effects on telomerase polymerization activity. Mol. Cell. Biol. 13:6586–6599.
   PubMedGoogle Scholar
• Lingner, J., T. R. Hughes, A. Shevchenko, M. Mann, V. Lundblad, and T. R. Cech. 1997. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276:561–567.
   PubMed Web of Science ®Google Scholar
• Lue, N. F. 2004. Adding to the ends: what makes telomerase processive and how important is it? Bioessays 26:955–962.
   PubMedGoogle Scholar
• Lue, N. F. 2005. A physical and functional constituent of telomerase anchor site. J. Biol. Chem. 280:26586–26591.
   PubMedGoogle Scholar
• Reference deleted.
   Google Scholar
• Marsilio, E., S. H. Cheng, B. Schaffhausen, E. Paucha, and D. M. Livingston. 1991. The T/t common region of simian virus 40 large T antigen contains a distinct transformation-governing sequence. J. Virol. 65:5647–5652.
   PubMedGoogle Scholar
• Meyerson, M., C. M. Counter, E. N. Eaton, L. W. Ellisen, P. Steiner, S. D. Caddle, L. Ziaugra, R. L. Beijersbergen, M. J. Davidoff, Q. Liu, S. Bacchetti, D. A. Haber, and R. A. Weinberg. 1997. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 90:785–795.
   PubMed Web of Science ®Google Scholar
• Moriarty, T. J., S. Huard, S. Dupuis, and C. Autexier. 2002. Functional multimerization of human telomerase requires an RNA interaction domain in the N terminus of the catalytic subunit. Mol. Cell. Biol. 22:1253–1265.
   PubMedGoogle Scholar
• Moriarty, T. J., D. T. Marie-Egyptienne, and C. Autexier. 2004. Functional organization of repeat addition processivity and DNA synthesis determinants in the human telomerase multimer. Mol. Cell. Biol. 24:3720–3733.
   PubMed Web of Science ®Google Scholar
• Nakamura, T. M., G. B. Morin, K. B. Chapman, S. L. Weinrich, W. H. Andrews, J. Lingner, C. B. Harley, and T. R. Cech. 1997. Telomerase catalytic subunit homologs from fission yeast and human. Science 277:955–959.
   PubMed Web of Science ®Google Scholar
• Nasir, L., E. Gault, S. Campbell, M. Veeramalai, D. Gilbert, R. McFarlane, A. Munro, and D. J. Argyle. 2004. Isolation and expression of the reverse transcriptase component of the Canis familiaris telomerase ribonucleoprotein (dogTERT). Gene 336:105–113.
   PubMedGoogle Scholar
• O'Connor, C. M., C. K. Lai, and K. Collins. 2005. Two purified domains of telomerase reverse transcriptase reconstitute sequence-specific interactions with RNA. J. Biol. Chem. 280:17533–17539.
   PubMedGoogle Scholar
• Rouda, S., and E. Skordalakes. 2007. Structure of the RNA-binding domain of telomerase: implications for RNA recognition and binding. Structure 15:1403–1412.
   PubMedGoogle Scholar
• Ware, T. L., H. Wang, and E. H. Blackburn. 2000. Three telomerases with completely non-telomeric template replacements are catalytically active. EMBO J. 19:3119–3131.
   PubMedGoogle Scholar
• Weinrich, S. L., R. Pruzan, L. Ma, M. Ouellette, V. M. Tesmer, S. E. Holt, A. G. Bodnar, S. Lichtsteiner, N. W. Kim, J. B. Trager, R. D. Taylor, R. Carlos, W. H. Andrews, W. E. Wright, J. W. Shay, C. B. Harley, and G. B. Morin. 1997. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. Nat. Genet. 17:498–502.
   PubMed Web of Science ®Google Scholar
• Wilson, I. A., D. H. Haft, E. D. Getzoff, J. A. Tainer, R. A. Lerner, and S. Brenner. 1985. Identical short peptide sequences in unrelated proteins can have different conformations: a testing ground for theories of immune recognition. Proc. Natl. Acad. Sci. U. S. A. 82:5255–5259.
   PubMedGoogle Scholar
• Wyatt, H. D., D. A. Lobb, and T. L. Beattie. 2007. Characterization of physical and functional anchor site interactions in human telomerase. Mol. Cell. Biol. 27:3226–3240.
   PubMedGoogle Scholar