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Article

Phosphorylation of Eukaryotic Elongation Factor 2 (eEF2) by Cyclin A–Cyclin-Dependent Kinase 2 Regulates Its Inhibition by eEF2 Kinase

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Pages 596-604 | Received 14 Sep 2012, Accepted 08 Nov 2012, Published online: 20 Mar 2023

REFERENCES

  • Celis JE, Madsen P, Ryazanov AG. 1990. Increased phosphorylation of elongation factor 2 during mitosis in transformed human amnion cells correlates with a decreased rate of protein synthesis. Proc. Natl. Acad. Sci. U. S. A. 87:4231–4235.
  • Pyronnet S, Sonenberg N. 2001. Cell-cycle-dependent translational control. Curr. Opin. Genet. Dev. 11:13–18.
  • Bonneau AM, Sonenberg N. 1987. Involvement of the 24-kDa cap-binding protein in regulation of protein synthesis in mitosis. J. Biol. Chem. 262:11134–11139.
  • Fan H, Penman S. 1970. Regulation of protein synthesis in mammalian cells. II. Inhibition of protein synthesis at the level of initiation during mitosis. J. Mol. Biol. 50:655–670.
  • Browne GJ, Proud CG. 2002. Regulation of peptide-chain elongation in mammalian cells. Eur. J. Biochem. 269:5360–5368.
  • Jørgensen R, Merrill AR, Andersen GR. 2006. The life and death of translation elongation factor 2. Biochem. Soc. Trans. 34:1–6.
  • Sivan G, Kedersha N, Elroy-Stein O. 2007. Ribosomal slowdown mediates translational arrest during cellular division. Mol. Cell. Biol. 27:6639–6646.
  • Ovchinnikov LP, Motuz LP, Natapov PG, Averbuch LJ, Wettenhall RE, Szyszka R, Kramer G, Hardesty B. 1990. Three phosphorylation sites in elongation factor 2. FEBS Lett. 275:209–212.
  • Price NT, Redpath NT, Severinov KV, Campbell DG, Russell JM, Proud CG. 1991. Identification of the phosphorylation sites in elongation factor-2 from rabbit reticulocytes. FEBS Lett. 282:253–258.
  • Nairn AC, Palfrey HC. 1987. Identification of the major Mr 100,000 substrate for calmodulin-dependent protein kinase III in mammalian cells as elongation factor-2. J. Biol. Chem. 262:17299–17303.
  • Ryazanov AG. 1987. Ca2+/calmodulin-dependent phosphorylation of elongation factor 2. FEBS Lett. 214:331–334.
  • Ryazanov AG, Davydova EK. 1989. Mechanism of elongation factor 2 (EF-2) inactivation upon phosphorylation. Phosphorylated EF-2 is unable to catalyze translocation. FEBS Lett. 251:187–190.
  • Proud CG. 2007. Signalling to translation: how signal transduction pathways control the protein synthetic machinery. Biochem. J. 403:217–234.
  • White-Gilbertson S, Kurtz DT, Voelkel-Johnson C. 2009. The role of protein synthesis in cell cycling and cancer. Mol. Oncol. 3:402–408.
  • Knebel A, Morrice N, Cohen P. 2001. A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta. EMBO J. 20:4360–4369.
  • Redpath NT, Foulstone EJ, Proud CG. 1996. Regulation of translation elongation factor-2 by insulin via a rapamycin-sensitive signalling pathway. EMBO J. 15:2291–2297.
  • Wang X, Li W, Williams M, Terada N, Alessi DR, Proud CG. 2001. Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase. EMBO J. 20:4370–4379.
  • Althausen S, Mengesdorf T, Mies G, Olah L, Nairn AC, Proud CG, Paschen W. 2001. Changes in the phosphorylation of initiation factor eIF-2alpha, elongation factor eEF-2 and p70 S6 kinase after transient focal cerebral ischaemia in mice. J. Neurochem. 78:779–787.
  • Browne GJ, Finn SG, Proud CG. 2004. Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398. J. Biol. Chem. 279:12220–12231.
  • Horman S, Browne G, Krause U, Patel J, Vertommen D, Bertrand L, Lavoinne A, Hue L, Proud C, Rider M. 2002. Activation of AMP-activated protein kinase leads to the phosphorylation of elongation factor 2 and an inhibition of protein synthesis. Curr. Biol. 12:1419–1423.
  • Redpath NT, Proud CG. 1993. Cyclic AMP-dependent protein kinase phosphorylates rabbit reticulocyte elongation factor-2 kinase and induces calcium-independent activity. Biochem. J. 293(Pt 1):31–34.
  • Chi Y, Welcker M, Hizli AA, Posakony JJ, Aebersold R, Clurman BE. 2008. Identification of CDK2 substrates in human cell lysates. Genome Biol. 9:R149. doi:10.1186/gb-2008-9-10-r149.
  • Dorovkov MV, Pavur KS, Petrov AN, Ryazanov AG. 2002. Regulation of elongation factor-2 kinase by pH. Biochemistry 41:13444–13450.
  • Clurman BE, Sheaff RJ, Thress K, Groudine M, Roberts JM. 1996. Turnover of cyclin E by the ubiquitin-proteasome pathway is regulated by cdk2 binding and cyclin phosphorylation. Genes Dev. 10:1979–1990.
  • Welcker M, Singer J, Loeb KR, Grim J, Bloecher A, Gurien-West M, Clurman BE, Roberts JM. 2003. Multisite phosphorylation by Cdk2 and GSK3 controls cyclin E degradation. Mol. Cell 12:381–392.
  • Drazin R, Kandel J, Collier RJ. 1971. Structure and activity of diphtheria toxin. II. Attack by trypsin at a specific site within the intact toxin molecule. J. Biol. Chem. 246:1504–1510.
  • Chi Y, Clurman BE. 2010. Mass spectrometry-based identification of protein kinase substrates utilizing engineered kinases and thiophosphate labeling. Curr. Protoc. Chem. Biol. 2:ch100151. doi:10.1002/9780470559277.ch100151.
  • Jackman M, Kubota Y, den Elzen N, Hagting A, Pines J. 2002. Cyclin A- and cyclin E-Cdk complexes shuttle between the nucleus and the cytoplasm. Mol. Biol. Cell 13:1030–1045.
  • Taylor DJ, Nilsson J, Merrill AR, Andersen GR, Nissen P, Frank J. 2007. Structures of modified eEF2 80S ribosome complexes reveal the role of GTP hydrolysis in translocation. EMBO J. 26:2421–2431.
  • Pavur KS, Petrov AN, Ryazanov AG. 2000. Mapping the functional domains of elongation factor-2 kinase. Biochemistry 39:12216–12224.
  • Hydbring P, Larsson LG. 2010. Tipping the balance: Cdk2 enables Myc to suppress senescence. Cancer Res. 70:6687–6691.
  • Sivan G, Elroy-Stein O. 2008. Regulation of mRNA translation during cellular division. Cell Cycle 7:741–744.
  • Smith EM, Proud CG. 2008. cdc2-cyclin B regulates eEF2 kinase activity in a cell cycle- and amino acid-dependent manner. EMBO J. 27:1005–1016.
  • Sivan G, Aviner R, Elroy-Stein O. 2011. Mitotic modulation of translation elongation factor 1 leads to hindered tRNA delivery to ribosomes. J. Biol. Chem. 286:27927–27935.

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