22
Views
41
CrossRef citations to date
0
Altmetric
Cell Growth and Development

Mutations in RNA Polymerase II and Elongation Factor SII Severely Reduce mRNA Levels in Saccharomyces cerevisiae

, , , &
Pages 5771-5779 | Received 06 Apr 1998, Accepted 02 Jul 1998, Published online: 28 Mar 2023

REFERENCES

  • Alani, E., L. Cao, and N. Kleckner 1987. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541–545.
  • Archambault, J., M. A. Drebot, J. C. Stone, and J. D. Friesen 1992. Isolation and phenotypic analysis of conditional-lethal, linker-insertion mutations in the gene encoding the largest subunit of RNA polymerase II in Saccharomyces cerevisiae. Mol. Gen. Genet. 232: 408–414.
  • Archambault, J., F. Lacroute, A. Ruet, and J. D. Friesen 1992. Genetic interaction between transcription elongation factor TFIIS and RNA polymerase II. Mol. Cell. Biol. 12: 4142–4152.
  • Aso, T., J. W. Conaway, and R. C. Conaway 1995. The RNA polymerase II elongation complex. FASEB J. 9: 1419–1428.
  • Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl 1988. Current protocols in molecular biology. Greene Publishing Associates/Wiley-Interscience, New York, N.Y.
  • Bengal, E., O. Flores, A. Krauskopf, D. Reinberg, and Y. Aloni 1991. Role of the mammalian transcription factors IIF, IIS, and IIX during elongation by RNA polymerase II. Mol. Cell. Biol. 11: 1195–1206.
  • Bradsher, J. N., S. Tan, H.-J. McLaury, J. W. Conaway, and R. C. Conaway 1993. RNA polymerase II transcription factor SIII. I. Identification, purification, and properties. J. Biol. Chem. 268: 25587–25593.
  • Choder, M. 1991. A general topoisomerase I-dependent transcriptional repression in the stationary phase in yeast. Genes Dev. 5: 2315–2326.
  • Choder, M., and R. A. Young 1993. A portion of RNA polymerase II molecules has a component essential for stress responses and stress survival. Mol. Cell. Biol. 13: 6984–6991.
  • Cormack, B. P., and K. Struhl 1992. The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells. Cell 69: 685–696.
  • DeRisi, J. L., V. R. Iyer, and P. O. Brown 1997. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278: 680–686.
  • Early, M. C., and G. F. Crouse 1996. Selectable cassettes for simplified construction of yeast gene disruption vectors. Gene 169: 111–113.
  • Exinger, G., and F. Lacroute 1992. 6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae. Curr. Genet. 22: 9–11.
  • Flores, O., I. Ha, and D. Reinberg 1990. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF. J. Biol. Chem. 265: 5629–5634.
  • Gietz, D., A. St. Jean, R. A. Woods, and R. H. Schiestl 1992. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 20: 1425.
  • Gu, W., and D. Reines 1995. Identification of a decay in transcription potential that results in elongation factor-dependence of RNA polymerase II. J. Biol. Chem. 270: 11238–11244.
  • Guzder, S. N., H. Qiu, C. H. Sommers, P. Sung, L. Prakash, and S. Prakash 1994. DNA repair gene RAD3 of S. cerevisiae is essential for transcription by RNA polymerase II. Nature 367: 91–94.
  • Hardwick, K. G., J. C. Boothroyd, A. D. Rudner, and H. R. B. Pelham 1992. Genes that allow yeast cells to grow in the absence of the HDEL receptor. EMBO J. 11: 4187–4195.
  • Hartzog, G. A., T. Wada, H. Handa, and F. Winston 1998. Evidence that SPT4, SPT5, and SPT6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev. 12: 357–369.
  • Herrick, D., R. Parker, and A. Jacobson 1990. Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae. Mol. Cell. Biol. 10: 2269–2284.
  • Hubert, J.-C., A. Guyonvarch, B. Kammerer, F. Exinger, P. Liljelund, and F. Lacroute 1983. Complete sequence of a eukaryotic regulatory gene. EMBO J. 2: 2071–2073.
  • Kashlev, M., J. Lee, K. Zalenskaya, V. Nikiforov, and A. Goldfarb 1990. Blocking of the initiation-to-elongation transition by a transdominant RNA polymerase mutation. Science 248: 1006–1009.
  • Landick, R., J. Stewart, and D. N. Lee 1990. Amino acid changes in conserved regions of the β-subunit of Escherichia coli RNA polymerase alter transcription pausing and termination. Genes Dev. 4: 1623–1636.
  • Lee, J. Y., K. Zalenskaya, Y. K. Shin, J. D. McKinney, J. H. Park, and A. Goldfarb 1989. Expression of cloned rpoB gene of Escherichia coli: a genetic system for the isolation of dominant negative mutations and overproduction of defective beta subunit of RNA polymerase. J. Bacteriol. 171: 3002–3007.
  • Nakanishi, T., A. Nakuno, K. Nomura, K. Sekimizu, and S. Natori 1992. Purification, gene cloning, and gene disruption of the transcription factor SII in Saccharomyces cerevisiae. J. Biol. Chem. 267: 13200–13204.
  • Nakanishi, T., M. Shimoaraiso, and S. Natori 1995. Structure-function relationship of yeast SII in terms of stimulation of RNA polymerase II, arrest relief, and suppression of 6-azauracil sensitivity. J. Biol. Chem. 270: 8991.
  • Powell, W., and D. Reines 1996. Mutations in the second largest subunit of RNA polymerase II cause 6-azauracil sensitivity in yeast and increased transcriptional arrest in vitro. J. Biol. Chem. 271: 6866–6873.
  • Price, D. H., A. E. Sluder, and A. L. Greenleaf 1989. Dynamic interaction between a Drosophila transcription factor and RNA polymerase II. Mol. Cell. Biol. 9: 1465–1475.8995.
  • Qiu, H., E. Park, L. Prakash, and S. Prakash 1993. The Saccharomyces cerevisiae DNA repair gene RAD25 is required for transcription by RNA polymerase II. Genes Dev. 7: 2161–2171.
  • Reines, D. 1994. Nascent RNA cleavage by transcription elongation complexes, p. 263–278. In: Conaway, R. C., and J. W. Conaway. Transcription mechanisms and regulation., Raven Press, New York, N.Y.
  • Reines, D., J. W. Conaway, and R. C. Conaway 1996. The RNA polymerase II general elongation factors. Trends Biochem. Sci. 21: 351–355.
  • Rose, M. D., P. Novick, J. H. Thomas, D. Bostein, and G. R. Fink 1987. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237–243.
  • Rothstein, R. 1991. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 194: 281–309.
  • Sagitov, V., V. Nikiforov, and A. Goldfarb 1993. Dominant lethal mutations near the 5′ substrate binding site affect RNA polymerase propagation. J. Biol. Chem. 268: 2195–2202.
  • Sakurai, H., T. Ohishi, and T. Fukasawa 1997. Promoter structure-dependent functioning of the general transcription factor IIE in Saccharomyces cerevisiae. J. Biol. Chem. 272: 15936–15942.
  • Scafe, C., M. Nonet, and R. A. Young 1990. RNA polymerase II mutants defective in transcription of a subset of genes. Mol. Cell. Biol. 10: 1010–1016.
  • Scafe, C., C. Martin, M. Nonet, S. Podos, S. Okamura, and R. A. Young 1990. Conditional mutations occur predominantly in highly conserved residues of RNA polymerase II subunits. Mol. Cell. Biol. 10: 1271–1275.
  • Sherman, F. 1991. Getting started with yeast. Methods Enzymol. 194: 3–20.
  • Shilatifard, A., W. S. Lane, K. W. Jackson, R. C. Conaway, and J. W. Conaway 1996. An RNA polymerase II elongation factor encoded by the human ELL gene. Science 271: 1873–1876.
  • Sikorski, R., and P. Hieter 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19–27.
  • Thompson, C. M., and R. A. Young 1995. General requirement for RNA polymerase II holoenzymes in vivo. Proc. Natl. Acad. Sci. USA 92: 4587–4590.
  • Tijerina, P., and M. H. Sayre 1998. A debilitating mutation in transcription factor IIE with differential effects on gene expression in yeast. J. Biol. Chem. 273: 1107–1113.
  • Uptain, S. M., C. M. Kane, and M. J. Chamberlin 1997. Basic mechanisms of transcript elongation and its regulation. Annu. Rev. Biochem. 66: 117–172.
  • Wang, H., S. E. Kohlami, and A. J. Cutler 1996. An improved method for polymerase chain reaction using whole yeast cells. Anal. Biochem. 237: 145–146.
  • Werner-Washburne, M., J. Becker, J. Kosic-Smithers, and E. A. Craig 1989. Yeast Hsp70 RNA levels vary in response to the physiological status of the cell. J. Bacteriol. 171: 2680–2688.
  • Werner-Washburne, M., E. Braun, G. C. Johnston, and R. A. Singer 1993. Stationary phase in the yeast Saccharomyces cerevisiae. Microbiol. Rev. 57: 383–401.
  • Wu, J., D. E. Awrey, A. M. Edwards, J. Archambault, and J. D. Friesen 1996. In vitro characterization of mutant yeast RNA polymerase II with reduced binding for elongation factor TFIIS. Proc. Natl. Acad. Sci. USA 93: 1152–1157.
  • Zaychikov, E., E. Martin, L. Denissova, M. Kozlov, V. Markovtsov, M. Kashlev, H. Heumann, V. Nikiforov, A. Goldfarb, and A. Mustaev 1996. Mapping of catalytic residues in the RNA polymerase active center. Science 273: 107–109.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.