83
Views
235
CrossRef citations to date
0
Altmetric
Cell Growth and Development

Targeted Inactivation of Mouse RAD52Reduces Homologous Recombination but Not Resistance to Ionizing Radiation

, , , , , , & show all
Pages 6423-6429 | Received 16 Apr 1998, Accepted 27 Jul 1998, Published online: 28 Mar 2023

REFERENCES

  • Akaboshi, E., Y. Inoue, and H. Ryo 1994. Cloning of the cDNA and genomic DNA that correspond to the recA-like gene of Drosophila melanogaster. Jpn. J. Genet. 69: 663–670.
  • Bai, Y., and L. S. Symington 1996. A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Genes Dev. 10: 2025–2037.
  • Baumann, P., F. E. Benson, and S. C. West 1996. Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell 87: 757–766.
  • Bendixen, C., I. Sunjevaric, R. Bauchwitz, and R. Rothstein 1994. Identification of a mouse homologue of the Saccharomyces cerevisiae recombination and repair gene, RAD52. Genomics 23: 300–303.
  • Bennett, C. B., A. L. Lewis, K. K. Baldwin, and M. A. Resnick 1993. Lethality induced by a single site-specific double-strand break in a dispensable yeast plasmid. Proc. Natl. Acad. Sci. USA 90: 5613–5617.
  • Benson, F. E., P. Baumann, and S. C. West 1998. Synergistic actions of Rad51 and Rad52 in recombination and DNA repair. Nature 391: 401–404.
  • Bezzubova, O. Y., A. Silbergleit, Y. Yamaguchi-Iwai, S. Takeda, and J.-M. Buerstedde 1997. Reduced X-ray resistance and homologous recombination frequencies in a RAD54−/− mutant of the chicken DT40 cell line. Cell 89: 185–193.
  • Bezzubova, O. Y., H. Schmidt, K. Ostermann, W.-D. Heyer, and J.-M. Buerstedde 1993. Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes. Nucleic Acids Res. 21: 5945–5949.
  • Boulton, S. J., and S. P. Jackson 1996. Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways. EMBO J. 15: 5093–5103.
  • Boulton, S. J., and S. P. Jackson 1998. Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J. 17: 1819–1828.
  • Chepurnaya, O. V., S. A. Kozhin, V. T. Peshekhonov, and V. G. Korolev 1995. RAD58 (XRS4)—a new gene in the RAD52 epistasis group. Curr. Genet. 28: 274–279.
  • Chu, C. C., W. E. Paul, and E. E. Max 1992. Quantitation of immunoglobulin μ-γ1 heavy chain switch region recombination by a digestion-circularization polymerase chain reaction method. Proc. Natl. Acad. Sci. USA 89: 6978–6982.
  • Contopoulou, C. R., V. E. Cook, and R. K. Mortimer 1987. Analysis of DNA double strand breakage and repair using orthogonal field alternation gel electrophoresis. Yeast 3: 71–76.
  • Essers, J., R. W. Hendriks, S. M. Swagemakers, C. Troelstra, J. de Wit, D. Bootsma, J. H. Hoeijmakers, and R. Kanaar 1997. Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination. Cell 89: 195–204.
  • Friedberg, E. C., G. C. Walker, and W. Siede 1995. DNA repair and mutagenesis. ASM Press, Washington, D.C.
  • Game, J. C. 1993. DNA double-strand breaks and the RAD50-RAD57 genes in Saccharomyces. Semin. Cancer Biol. 4: 73–83.
  • Golub, E. I., O. V. Kovalenko, R. C. Gupta, D. C. Ward, and C. M. Radding 1997. Interaction of human recombination proteins Rad51 and Rad54. Nucleic Acids Res. 25: 4106–4110.
  • Jeggo, P. A., G. E. Taccioli, and S. P. Jackson 1995. Menage a trois: double strand break repair, V(D)J recombination and DNA-PK. Bioessays 17: 949–957.
  • Johnson, B. L., B. Thyagarajan, L. Krueger, B. Hirsch, and C. Campbell 1996. Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene. Mutat. Res. 363: 179–189.
  • Joyner, A. L. 1993. Gene targeting: a practical approach. Oxford University Press Inc, New York, N.Y.
  • Klein, H. L. 1997. RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis. Genetics 147: 1533–1543.
  • Koken, M. H., P. Reynolds, I. Jaspers-Dekker, L. Prakash, S. Prakash, D. Bootsma, and J. H. Hoeijmakers 1991. Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6. Proc. Natl. Acad. Sci. USA 88: 8865–8869.
  • Kooistra, R., K. Vreeken, J. B. Zonneveld, A. de Jong, J. C. Eeken, C. J. Osgood, J. M. Buerstedde, P. H. Lohman, and A. Pastink 1997. The Drosophila melanogaster RAD54 homolog, DmRAD54, is involved in the repair of radiation damage and recombination. Mol. Cell. Biol. 17: 6097–6104.
  • Lim, D.-S., and P. Hasty 1996. A mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53. Mol. Cell. Biol. 16: 7133–7143.
  • Masutani, C., K. Sugasawa, J. Yanagisawa, T. Sonoyama, M. Ui, T. Enemoto, K. Takio, K. Tanaka, P. J. van der Spek, D. Bootsma, J. H. J. Hoeijmakers, and F. Hanaoka 1994. Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23. EMBO J. 13: 1831–1843.
  • McKee, B. D., X.-J. Ren, and C.-S. Hong 1996. A recA-like gene in Drosophila melanogaster that is expressed at high levels in female but not male meiotic tissues. Chromosoma 104: 479–488.
  • Milne, G. T., S. Jin, K. B. Shannon, and D. T. Weaver 1996. Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae. Mol. Cell. Biol. 16: 4189–4198.
  • Moore, J. K., and J. E. Haber 1996. Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae. Mol. Cell. Biol. 16: 2164–2173.
  • Muris, D. F., O. Bezzubova, J.-M. Buerstedde, K. Vreeken, A. S. Balajee, C. J. Osgood, C. Troelstra, J. H. Hoeijmakers, K. Ostermann, H. Schmidt, A. T. Natarajan, J. C. J. Eeken, P. H. M. Lohman, and A. Pastink 1994. Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination. Mutat. Res. 315: 295–305.
  • Muris, D. F., K. Vreeken, A. M. Carr, J. M. Murray, C. Smit, P. H. Lohman, and A. Pastink 1996. Isolation of the Schizosaccharomyces pombe RAD54 homologue, rhp54+, a gene involved in the repair of radiation damage and replication fidelity. J. Cell Sci. 109: 73–81.
  • Muris, D. F. R., K. Vreeken, H. Schmidt, K. Ostermann, B. Clever, P. H. M. Lohman, and A. Pastink 1997. Homologous recombination in the fission yeast Schizosaccharomyces pombe: different requirements for the rhp51+, rhp54+, and rad22+genes. Curr. Genet. 31: 248–254.
  • New, J. H., T. Sugiyama, E. Zaitseva, and S. C. Kowalczykowski 1998. Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature 391: 407–410.
  • Ostermann, K., A. Lorentz, and H. Schmidt 1993. The fission yeast rad22 gene, having a function in mating-type switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae. Nucleic Acids Res. 21: 5940–5944.
  • Park, M. S. 1995. Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells. J. Biol. Chem. 270: 15467–15470.
  • Petrini, J. H., D. A. Bressan, and M. S. Yao 1997. The RAD52 epistasis group in mammalian double strand break repair. Semin. Immunol. 9: 181–188.
  • Petukhova, G., S. Stratton, and P. Sung 1998. Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins. Nature 393: 91–94.
  • Reis, L. F., H. Ruffner, G. Stark, M. Aguet, and C. Weissmann 1994. Mice devoid of interferon regulatory factor 1 (IRF-1) show normal expression of type I interferon genes. EMBO J. 13: 4798–4806.
  • Roest, H. P., J. van Klaveren, J. de Wit, C. G. van Gurp, M. H. Koken, M. Vermey, J. H. van Roijen, J. W. Hoogerbrugge, J. T. Vreeburg, W. M. Baarends, D. Bootsma, J. A. Grootegoed, and J. H. Hoeijmakers 1996. Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification. Cell 86: 799–810.
  • Rolink, A., F. Melchers, and J. Andersson 1996. The SCID but not the RAG-2 gene product is required for Sμ-Sɛ heavy chain class switching. Immunity 5: 319–330.
  • Roller, M. L., A. C. Lossie, M. H. Koken, E. M. Smit, A. Hagemeijer, and S. A. Camper 1995. Localization of sequences related to the human RAD6 DNA repair gene on mouse chromosomes 11 and 13. Mamm. Genome 6: 305–306.
  • Roth, D. B., T. Lindahl, and M. Gellert 1995. Repair and recombination. How to make ends meet. Curr. Biol. 5: 496–499.
  • Shen, Z., K. G. Cloud, D. J. Chen, and M. S. Park 1996. Specific interactions between the human RAD51 and RAD52 proteins. J. Biol. Chem. 271: 148–152.
  • Shen, Z., K. Denison, R. Lobb, J. M. Gatewood, and D. J. Chen 1995. The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues. Genomics 25: 199–206.
  • Shinohara, A., and T. Ogawa 1995. Homologous recombination and the roles of double-strand breaks. Trends Biochem. Sci. 20: 387–391.
  • Shinohara, A., and T. Ogawa 1998. Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature 391: 404–407.
  • Shinohara, M., E. Shita-Yamaguchi, J. M. Buerstedde, H. Shinagawa, H. Ogawa, and A. Shinohara 1997. Characterization of the roles of the Saccharomyces cerevisiae RAD54 gene and a homologue of RAD54, RDH54/TID1, in mitosis and meiosis. Genetics 147: 1545–1556.
  • Siede, W., A. A. Friedl, I. Dianova, F. Eckardt-Schupp, and E. C. Friedberg 1996. The Saccharomyces cerevisiae Ku autoantigen homologue affects radiosensitivity only in the absence of homologous recombination. Genetics 142: 91–102.
  • Sonoda, E., M. S. Sasaki, J.-M. Buerstedde, O. Bezzubova, A. Shinohara, H. Ogawa, M. Takata, Y. Yamaguchi-Iwai, and S. Takeda 1998. Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death. EMBO J. 17: 598–608.
  • Sung, P. 1997. Function of yeast Rad52 protein as a mediator between replication protein A and the Rad51 recombinase. J. Biol. Chem. 272: 28194–28197.
  • Sung, P. 1997. Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase. Genes Dev. 11: 1111–1121.
  • Sung, P., and D. L. Robberson 1995. DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA. Cell 82: 453–461.
  • Taki, T., T. Ohnishi, A. Yamamoto, S. Hiraga, N. Arita, S. Izumoto, T. Hayakawa, and T. Morita 1996. Antisense inhibition of the RAD51 enhances radiosensitivity. Biochem. Biophys. Res. Commun. 223: 434–438.
  • Tavassoli, M., M. Shayeghi, A. Nasim, and F. Z. Watts 1995. Cloning and characterisation of the Schizosaccharomyces pombe rad32 gene: a gene required for repair of double-strand breaks and recombination. Nucleic Acids Res. 23: 383–388.
  • te Riele, H., E. R. Maandag, and A. Berns 1992. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc. Natl. Acad. Sci. USA 89: 5128–5132.
  • Tsukamoto, Y., J. Kato, and H. Ikeda 1997. Budding yeast Rad50, Mre11, Xrs2, and Hdf1, but not Rad52, are involved in the formation of deletions on a dicentric plasmid. Mol. Gen. Genet. 255: 543–547.
  • Tsuzuki, T., Y. Fujii, K. Sakumi, Y. Tominaga, K. Nakao, M. Sekiguchi, A. Matsushiro, Y. Yoshimura, and T. Morita 1996. Targeted disruption of the Rad51 gene leads to lethality in embryonic mice. Proc. Natl. Acad. Sci. USA 93: 6236–6240.
  • van den Ouweland, J., T. Rijkers, and A. Pastink 1997. Genomic characterization of the mouse homolog of the Saccharomyces cerevisiae recombination and double-strand break repair gene RAD52. Mutat. Res. 383: 125–135.
  • van der Horst, G. T., H. van Steeg, R. J. Berg, A. J. van Gool, J. de Wit, G. Weeda, H. Morreau, R. B. Beems, C. F. van Kreijl, F. R. de Gruijl, D. Bootsma, and J. H. Hoeijmakers 1997. Defective transcription-coupled repair in Cockayne syndrome B mice is associated with skin cancer predisposition. Cell 89: 425–435.
  • van der Spek, P. J., C. E. Visser, F. Hanaoka, B. Smit, A. Hagemeijer, D. Bootsma, and J. H. Hoeijmakers 1996. Cloning, comparative mapping, and RNA expression of the mouse homologues of the S. cerevisiae nucleotide excision repair gene RAD23. Genomics 31: 20–27.
  • Xu, L., and P. Rothman 1994. IFN-γ represses ɛ germline transcription and subsequently down-regulates switch recombination to ɛ. Int. Immunol. 6: 515–521.
  • Yamaguchi-Iwai, Y., E. Sonoda, J.-M. Buerstedde, O. Bezzubova, C. Morrison, M. Takata, A. Shinohara, and S. Takeda 1998. Homologous recombination, but not DNA repair, is reduced in vertebrate cells deficient in RAD52. Mol. Cell. Biol. 18: 6430–6435.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.