Dorsal-Mediated Repression Requires the Formation of a Multiprotein Repression Complex at the Ventral Silencer

REFERENCES

• Akimaru, H., D. X. Hou, and S. Ishii 1997. Drosophila CBP is required for dorsal-dependent twist gene expression. Nat. Genet. 17: 211–214.
   PubMedGoogle Scholar
• Belvin, M. P., and K. V. Anderson 1996. A conserved signaling pathway: the Drosophila toll-dorsal pathway. Annu. Rev. Cell Dev. Biol. 12: 393–416.
   PubMed Web of Science ®Google Scholar
• Biggin, M. D., and W. McGinnis 1997. Regulation of segmentation and segmental identity by Drosophila homeoproteins: the role of DNA binding in functional activity and specificity. Development 124: 4425–4433.
   PubMedGoogle Scholar
• Blochlinger, K., R. Bodmer, J. Jack, L. Y. Jan, and Y. N. Jan 1988. Primary structure and expression of a product from cut, a locus involved in specifying sensory organ identity in Drosophila. Nature 333: 629–635.
   PubMedGoogle Scholar
• Blochlinger, K., L. Y. Jan, and Y. N. Jan 1991. Transformation of sensory organ identity by ectopic expression of Cut in Drosophila. Genes Dev. 5: 1124–1135.
   PubMedGoogle Scholar
• Bodmer, R., S. Barbel, S. Shepard, J. W. Jack, L. Y. Jan, and Y. N. Jan 1987. Transformation of sensory organs by mutations of the cut locus of D. melanogaster. Cell 51: 293–307.
   PubMedGoogle Scholar
• Cai, H. N., D. N. Arnosti, and M. Levine 1996. Long-range repression in the Drosophila embryo. Proc. Natl. Acad. Sci. USA 93: 9309–9314.
   PubMedGoogle Scholar
• Campos-Ortega, J. A., and V. Hartenstein 1985. The embryonic development of Drosophila melanogaster. Springer-Verlag, Berlin, Germany.
   Google Scholar
• Chou, T. B., and N. Perrimon 1992. Use of a yeast site-specific recombinase to produce female germ line chimeras in Drosophila. Genetics 131: 643–653.
   PubMed Web of Science ®Google Scholar
• Courey, A. J., and J. Huang 1995. The establishment and interpretation of transcription factor gradients in the Drosophila embryo. Biochim. Biophys. Acta 1261: 1–18.
   PubMedGoogle Scholar
• Dubnicoff, T., S. A. Valentine, G. Chen, T. Shi, J. A. Lengyel, Z. Paroush, and A. J. Courey 1997. Conversion of dorsal from an activator to a repressor by the global corepressor Groucho. Genes Dev. 11: 2952–2957.
   PubMed Web of Science ®Google Scholar
• Dupfort, D., and A. Nepveu 1994. The human Cut homeodomain protein represses transcription from the c-myc promoter. Mol. Cell. Biol. 14: 4251–4257.
   PubMedGoogle Scholar
• Fernandez, J., L. Andrews, and S. M. Mische 1994. An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis. Anal. Biochem. 218: 112–117.
   PubMedGoogle Scholar
• Govind, S., and R. Steward 1991. Dorsoventral pattern formation in Drosophila: signal transduction and nuclear targeting. Trends Genet. 7: 119–125.
   PubMedGoogle Scholar
• Gregory, S. L., R. D. Kortschak, B. Kalionis, and R. Saint 1996. Characterization of the dead ringer gene identifies a novel, highly conserved family of sequence-specific DNA-binding proteins. Mol. Cell. Biol. 16: 792–799.
   PubMedGoogle Scholar
• Herrscher, R. F., M. H. Kaplan, D. L. Lelsz, C. Das, R. Scheuermann, and P. W. Tucker 1995. The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family. Genes Dev. 9: 3067–3082.
   PubMedGoogle Scholar
• Huang, J., D. H. Schwyter, J. M. Shirokawa, and A. J. Courey 1993. The interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression. Genes Dev. 7: 694–704.
   PubMedGoogle Scholar
• Huang, J. D., T. Dubnicoff, G. J. Liaw, Y. Bai, S. A. Valentine, J. M. Shirokawa, J. A. Lengyel, and A. J. Courey 1995. Binding sites for transcription factor NTF-1/Elf-1 contribute to the ventral repression of decapentaplegic. Genes Dev. 9: 3177–3189.
   PubMedGoogle Scholar
• Ip, Y. T., R. Kraut, M. Levine, and C. A. Rushlow 1991. The dorsal morphogen is a sequence-specific DNA-binding protein that interacts with a long-range repression element in Drosophila. Cell 64: 439–446.
   PubMedGoogle Scholar
• Jack, J. W. 1985. Molecular organization of the cut locus of Drosophila melanogaster. Cell 42: 869–876.
   PubMedGoogle Scholar
• Jiang, J., D. Kosman, Y. T. Ip, and M. Levine 1991. The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos. Genes Dev. 5: 1881–1891.
   PubMed Web of Science ®Google Scholar
• Jiang, J., C. A. Rushlow, Q. Zhou, S. Small, and M. Levine 1992. Individual dorsal morphogen binding sites mediate activation and repression in the Drosophila embryo. EMBO J. 11: 3147–3154.
   PubMedGoogle Scholar
• Jiang, J., H. Cai, Q. Zhou, and M. Levine 1993. Conversion of a dorsal-dependent silencer into an enhancer: evidence for dorsal corepressors. EMBO J. 12: 3201–3209.
   PubMedGoogle Scholar
• Kadonaga, J. T., and R. Tjian 1986. Affinity purification of sequence-specific DNA binding proteins. Proc. Natl. Acad. Sci. USA 83: 5889–5893.
   PubMed Web of Science ®Google Scholar
• Kirov, N., L. Zhelnin, J. Shah, and C. Rushlow 1993. Conversion of a silencer into an enhancer: evidence for a co-repressor in dorsal-mediated repression in Drosophila. EMBO J. 12: 3193–3199.
   PubMedGoogle Scholar
• Kirov, N. C., P. M. Lieberman, and C. Rushlow 1996. The transcriptional corepressor DSP1 inhibits activated transcription by disrupting TFIIA-TBP complex formation. EMBO J. 15: 7079–7087.
   PubMedGoogle Scholar
• Kissinger, C. R., B. Liu, E. Martin-Blanco, T. B. Kornberg, and C. O. Pabo 1990. Crystal structure of an engrailed homeodomain-DNA complex at 2.8 A resolution: a framework for understanding homeodomain-DNA interactions. Cell 63: 579–590.
   PubMed Web of Science ®Google Scholar
• Laney, J. D., and M. D. Biggin 1996. Redundant control of Ultrabithorax by zeste involves functional levels of zeste protein binding at the Ultrabithorax promoter. Development 122: 2303–2311.
   PubMedGoogle Scholar
• Lehming, N., D. Thanos, J. M. Brickman, J. Ma, T. Maniatis, and M. Ptashne 1994. An HMG-like protein that can switch a transcriptional activator to a repressor. Nature 371: 175–179.
   PubMed Web of Science ®Google Scholar
• Liaw, G. J., and J. A. Lengyel 1993. Control of tailless expression by bicoid, dorsal and synergistically interacting terminal system regulatory elements. Mech. Dev. 40: 47–61.
   PubMedGoogle Scholar
• Mailly, F., G. Berube, R. Harada, P. L. Mao, S. Phillips, and A. Nepveu 1996. The human cut homeodomain protein can repress gene expression by two distinct mechanisms: active repression and competition for binding site occupancy. Mol. Cell. Biol. 16: 5346–5357.
   PubMed Web of Science ®Google Scholar
• Palaparti, A., A. Baratz, and S. Stifani 1997. The Groucho/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3. J. Biol. Chem. 272: 26604–26610.
   PubMedGoogle Scholar
• Pan, D. J., J. D. Huang, and A. J. Courey 1991. Functional analysis of the Drosophila twist promoter reveals a dorsal-binding ventral activator region. Genes Dev. 5: 1892–1901.
   PubMedGoogle Scholar
• Pan, D. J., and A. J. Courey 1992. The same dorsal binding site mediates both activation and repression in a context-dependent manner. EMBO J. 11: 1837–1842.
   PubMedGoogle Scholar
• Pan, D., S. A. Valentine, and A. J. Courey 1994. The bipartite D. melanogaster twist promoter is reorganized in D. virilis. Mech. Dev. 46: 41–53.
   PubMedGoogle Scholar
• Paroush, Z., Finley, R.Jr., T. Kidd, S. Wainwright, P. Ingham, R. Brent, and D. Ish-Horowicz 1994. Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with Hairy-related bHLH proteins. Cell 79: 805–815.
   PubMed Web of Science ®Google Scholar
• Ray, R. P., K. Arora, C. Nüsslein-Volhard, and W. M. Gelbart 1991. The control of cell fate along the dorsal-ventral axis of the Drosophila embryo. Development 113: 35–54.
   PubMed Web of Science ®Google Scholar
• Roth, S., D. Stein, and C. Nüsslein-Volhard 1989. A gradient of nuclear localization of the dorsal protein determines dorsoventral pattern in the Drosophila embryo. Cell 59: 1189–1202.
   PubMed Web of Science ®Google Scholar
• Rubin, G. M., and A. C. Spradling 1982. Genetic transformation of Drosophila with transposable element vectors. Science 218: 348–353.
   PubMed Web of Science ®Google Scholar
• Rushlow, C. A., D. Han, J. S. Manley, and M. Levine 1989. The graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila. Cell 59: 1165–1177.
   PubMedGoogle Scholar
• Shandala, T., and R. Saint. Unpublished data.
   Google Scholar
• Shirokawa, J. M., and A. J. Courey 1997. A direct contact between the Dorsal rel homology domain and Twist may mediate transcriptional synergy. Mol. Cell. Biol. 17: 3345–3355.
   PubMedGoogle Scholar
• Simon, J., M. Peifer, W. Bender, and M. O’Connor 1990. Regulatory elements of the bithorax complex that control expression along the anterior-posterior axis. EMBO J. 9: 3945–3956.
   PubMedGoogle Scholar
• Soeller, W. C., S. J. Poole, and T. Kornberg 1988. In vitro transcription of the Drosophila engrailed gene. Genes Dev. 2: 68–81.
   PubMedGoogle Scholar
• Steward, R. 1989. Relocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its function. Cell 59: 1179–1188.
   PubMed Web of Science ®Google Scholar
• Tautz, D., and C. Pfeifle 1989. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98: 81–85.
   PubMed Web of Science ®Google Scholar
• Thisse, C., F. Perrin-Schmitt, C. Stoetzel, and B. Thisse 1991. Sequence-specific transactivation of the Drosophila twist gene by the dorsal gene product. Cell 65: 1191–1201.
   PubMedGoogle Scholar
• Treisman, J. E., A. Luk, G. M. Rubin, and U. Heberlein 1997. Eyelid antagonizes wingless signaling during Drosophila development and has homology to the Bright family of DNA-binding proteins. Genes Dev. 11: 1949–1962.
   PubMedGoogle Scholar