Inactivation of p16 in Human Mammary Epithelial Cells by CpG Island Methylation

REFERENCES

• Alcorta, D. A., Y. Xiong, D. Phelps, G. Hannon, D. Beach, and J. C. Barrett 1996. Involvement of the cyclin-dependent kinase inhibitor p16 (INK4a) in replicative senescence of normal human fibroblasts. Proc. Natl. Acad. Sci. USA 93: 13742–13747.
   PubMed Web of Science ®Google Scholar
• Band, V., J. A. De Caprio, L. Delmolino, V. Kulesa, and R. Sager 1991. Loss of p53 protein in human papillomavirus type 16 E6-immortalized human mammary epithelial cells. J. Virol. 65: 6671–6676.
   PubMedGoogle Scholar
• Band, V., and R. Sager 1989. Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types. Proc. Natl. Acad. Sci. USA 86: 1249–1253.
   PubMed Web of Science ®Google Scholar
• Banks, L., C. Edmonds, and K. H. Vousden 1990. Ability of the HPV16 E7 protein to bind RB and induce DNA synthesis is not sufficient for efficient transforming activity in NIH3T3 cells. Oncogene 5: 1383–1389.
   PubMedGoogle Scholar
• Barrett, M. T., B. J. Reid, and G. Joslyn 1995. Genotypic analysis of multiple loci in somatic cells by whole genome amplification. Nucleic Acids Res. 23: 3488–3492.
   PubMedGoogle Scholar
• Barrett, M. T., C. A. Sanchez, P. C. Galipeau, K. Neshat, M. Emond, and B. J. Reid 1996. Allelic loss of 9p21 and mutation of the CDKN2/p16 gene develop as early lesions during neoplastic progression in Barrett’s esophagus. Oncogene 13: 1867–1873.
   PubMed Web of Science ®Google Scholar
• Brenner, A. J., and C. M. Aldaz 1995. Chromosome 9p allelic loss and p16/CDKN2 in breast cancer and evidence of p16 inactivation in immortal breast epithelial cells. Cancer Res. 55: 2892–2895.
   PubMedGoogle Scholar
• Buckley, M. F., K. J. E. Sweeney, J. A. Hamilton, R. L. Sini, D. L. Manning, R. I. Nicholson, A. deFazio, C. K. W. Watts, E. A. Musgrove, and R. L. Sutherland 1993. Expression and amplification of cyclin genes in human breast cancer. Oncogene 8: 2127–2133.
   PubMed Web of Science ®Google Scholar
• Chuang, L. S.-H., H.-I. Ian, T.-W. Koh, H.-H. Ng, G. Xu, and B. F. L. Li 1997. Human DNA-(cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1. Science 277: 1996–2000.
   PubMed Web of Science ®Google Scholar
• Davies, R., R. Hicks, T. Crook, J. Morris, and K. Vousden 1993. Human papillomavirus type 16 E7 associates with a histone H1 kinase and with p107 through sequences necessary for transformation. J. Virol. 67: 2521–2528.
   PubMedGoogle Scholar
• Demers, G. W., E. Espling, J. B. Harry, B. G. Etscheid, and D. A. Galloway 1996. Abrogation of growth arrest signals by human papillomavirus type 16 E7 is mediated by sequences required for transformation. J. Virol. 70: 6862–6869.
   PubMedGoogle Scholar
• Dyson, N., P. M. Howley, K. Munger, and E. Harlow 1989. The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243: 934–937.
   PubMed Web of Science ®Google Scholar
• Ewen, M. E. 1994. The cell cycle and the retinoblastoma protein family. Cancer Metastasis Rev. 13: 45–66.
   PubMed Web of Science ®Google Scholar
• Foster, S. A., and D. A. Galloway. 1996. Unpublished data.
   Google Scholar
• Foster, S. A., and D. A. Galloway 1996. Human papillomavirus type 16 E7 alleviates a proliferation block in early passage human mammary epithelial cells. Oncogene 12: 1773–1779.
   PubMedGoogle Scholar
• Funk, J. O., S. Waga, J. B. Harry, E. Espling, B. Stillman, and D. A. Galloway 1997. Inhibition of CDK activity and PCNA-dependent DNA replication by p21 is blocked by interaction with the HPV 16 E7 oncoprotein. Genes Dev. 11: 2090–2100.
   PubMed Web of Science ®Google Scholar
• Garrett, L. R., N. Perez-Reyes, P. P. Smith, and J. K. McDougall 1993. Interaction of HPV-18 and nitrosomethylurea in the induction of squamous cell carcinoma. Carcinogenesis 14: 329–332.
   PubMedGoogle Scholar
• Glisin, V., R. Crkvenjakov, and C. Byus 1974. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13: 2633.
   PubMed Web of Science ®Google Scholar
• Halbert, C. L., G. W. Demers, and D. A. Galloway 1991. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473–478.
   PubMed Web of Science ®Google Scholar
• Hannon, G. J., and D. Beach 1994. p15INK4B is a potential effector of TGF-β-induced cell cycle arrest. Nature 371: 257–261.
   PubMed Web of Science ®Google Scholar
• Hara, E., R. Smith, D. Parry, H. Tahara, S. Stone, and G. Peters 1996. Regulation of p16CDKN2 expression and its implications for cell immortalization and senescence. Mol. Cell. Biol. 16: 859–867.
   PubMed Web of Science ®Google Scholar
• Hawley-Nelson, P., K. H. Vousden, N. L. Hubbert, D. R. Lowy, and J. T. Schiller 1989. HPV 16 E6 and E7 proteins cooperate to immortalize primary human foreskin keratinocytes. EMBO J. 8: 3905–3910.
   PubMed Web of Science ®Google Scholar
• Herman, J. G., J. R. Graff, S. Myohanen, B. D. Nelkin, and S. B. Baylin 1996. Methylation-specific PCR: a novel assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. USA 93: 9821–9826.
   PubMed Web of Science ®Google Scholar
• Herman, J. G., J. Jen, A. Merlo, and S. B. Baylin 1996. Hypermethylation-associated inactivation indicates a tumor suppressor role for p15INK4B. Cancer Res. 56: 722–727.
   PubMed Web of Science ®Google Scholar
• Herman, J. G., A. Merlo, L. Mao, R. G. Lapidus, J. P. J. Issa, N. E. Davidson, D. Sidransky, and S. B. Baylin 1995. Inactivation of the CDKN2/p16/MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers. Cancer Res. 55: 4525–4530.
   PubMed Web of Science ®Google Scholar
• Hurlin, P. J., P. Kaur, P. P. Smith, N. Perez-Reyes, R. A. Blanton, and J. K. McDougall 1991. Progression of human papillomavirus type 18-immortalized human keratinocytes to a malignant phenotype. Proc. Natl. Acad. Sci. USA 88: 570–574.
   PubMed Web of Science ®Google Scholar
• Jewers, R. J., P. Hildebrandt, J. W. Ludlow, B. Kell, and D. J. McCance 1992. Regions of human papillomavirus type 16 E7 oncoprotein required for immortalization of human keratinocytes. J. Virol. 66: 1329–1335.
   PubMedGoogle Scholar
• Jones, P. A. 1996. DNA methylation errors and cancer. Cancer Res. 56: 2463–2467.
   PubMed Web of Science ®Google Scholar
• Kamb, A., N. A. Gruis, J. Weaver-Feldhaus, Q. Liu, K. Harshman, S. V. Tavtigian, E. Stockert, R. S. Day, B. E. Johnson, and M. H. Skolnick 1994. A cell cycle regulator potentially involved in genesis of many tumor types. Science 264: 436–440.
   PubMed Web of Science ®Google Scholar
• Keyomarsi, K., and A. B. Pardee 1993. Redundant cyclin overexpression and gene amplification in breast cancer cells. Proc. Natl. Acad. Sci. USA 90: 1112–1116.
   PubMed Web of Science ®Google Scholar
• Klingelhutz, A. J., P. P. Smith, L. R. Garrett, and J. K. McDougall 1993. Alteration of the DCC tumor-suppressor gene in tumorigenic HPV-18 immortalized human keratinocytes transformed by nitrosomethylurea. Oncogene 8: 95–99.
   PubMedGoogle Scholar
• Koh, J., G. H. Enders, B. D. Dynlacht, and E. Harlow 1995. Tumour-derived p16 alleles encoding proteins defective in cell-cycle inhibition. Nature 375: 506–510.
   PubMed Web of Science ®Google Scholar
• Laborda, J. 1991. 36B4 cDNA used as an estradiol-independent mRNA control is the cDNA for human acidic phosphoprotein PO. Nucleic Acids Res. 19: 3998.
   PubMed Web of Science ®Google Scholar
• Loughran, O., A. Malliri, D. Owens, P. H. Gallimore, M. A. Stanley, B. Ozanne, M. C. Frame, and E. K. Parkinson 1996. Association of CDKN2A/p16INK4A with human head and neck keratinocyte replicative senescence: relationship of dysfunction to immortality and neoplasia. Oncogene 13: 561–568.
   PubMed Web of Science ®Google Scholar
• Lukas, J., J. Bartkova, M. Rohde, M. Strauss, and J. Bartek 1995. Cyclin D1 is dispensable for G1 control in retinoblastoma gene-deficient cells independently of cdk4 activity. Mol. Cell. Biol. 15: 2600–2611.
   PubMed Web of Science ®Google Scholar
• Lukas, J., D. Parry, L. Aagaard, D. J. Mann, J. Bartkova, M. Strauss, G. Peters, and J. Bartek 1995. Retinoblastoma-protein-dependent cell-cycle inhibition by the tumor suppressor p16. Nature 375: 503–506.
   PubMed Web of Science ®Google Scholar
• Medema, R. H., R. E. Herrera, F. Lam, and R. A. Weinberg 1995. Growth suppression by p16ink4 requires functional retinoblastoma protein. Proc. Natl. Acad. Sci. USA 92: 6289–6293.
   PubMed Web of Science ®Google Scholar
• Merlo, A., J. G. Herman, L. Mao, D. J. Lee, E. Gabrielson, P. C. Burger, S. B. Baylin, and D. Sidransky 1995. 5′ CpG island methylation is associated with transcriptional silencing of the tumor suppressor p16/CDKN2/MTS1 in human cancers. Nat. Med. 1: 686–692.
   PubMed Web of Science ®Google Scholar
• Miller, A. D., and G. J. Rosman 1989. Improved retroviral vectors for gene transfer and expression. BioTechniques 7: 980–990.
   PubMed Web of Science ®Google Scholar
• Munger, K., W. C. Phelps, V. Bubb, P. M. Howley, and R. Schlegel 1989. The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. J. Virol. 63: 4417–4421.
   PubMed Web of Science ®Google Scholar
• Munger, K., B. A. Werness, N. Dyson, W. C. Phelps, E. Harlow, and P. M. Howley 1989. Complex formation of the human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J. 8: 4099–4105.
   PubMed Web of Science ®Google Scholar
• Noble, J. R., E. M. Rogan, A. A. Neumann, K. Maclean, T. M. Bryan, and R. R. Reddel 1996. Association of extended in vitro proliferative potential with loss of p16INK4 expression. Oncogene 13: 1259–1268.
   PubMedGoogle Scholar
• Nobori, T., K. Miura, D. J. Wu, A. Lois, K. Takabayashi, and D. A. Carson 1994. Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers. Nature 368: 753–756.
   PubMed Web of Science ®Google Scholar
• Otsuki, T., H. M. Clark, A. Wellmann, E. S. Jaffe, and M. Raffeld 1995. Involvement of CDKN2 (p16INK4A/MTS1) and p15INK4B/MTS2 in human leukemias and lymphomas. Cancer Res. 55: 1436–1440.
   PubMed Web of Science ®Google Scholar
• Reed, A., J. Califano, P. Cairns, W. H. Westra, R. M. Jones, W. Koch, S. Ahrendt, Y. Eby, D. Sewell, H. Nawroz, J. Bartek, and D. Sidransky 1996. High frequency of p16 (CDKN2/MTS-1/INK4A) inactivation in head and neck squamous cell carcinoma. Cancer Res. 56: 3630–3633.
   PubMed Web of Science ®Google Scholar
• Reznikoff, C. A., T. R. Yeager, C. D. Belair, E. Savelieva, J. A. Puthenveettil, and W. M. Stadler 1996. Elevated p16 at senescence and loss of p16 at immortalization in human papillomavirus 16 E6, but not E7, transformed human uroepithelial cells. Cancer Res. 56: 2886–2890.
   PubMed Web of Science ®Google Scholar
• Scheffner, M., B. A. Werness, J. M. Huibregtse, A. J. Levine, and P. M. Howley 1990. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell 63: 1129–1136.
   PubMed Web of Science ®Google Scholar
• Serrano, M., G. J. Hannon, and D. Beach 1993. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature 366: 704–707.
   PubMed Web of Science ®Google Scholar
• Shay, J. W., W. E. Wright, D. Brasiskyte, and B. A. Van Der Haegen 1993. E6 of human papillomavirus type 16 can overcome the M1 stage of immortalization in human mammary epithelial cells but not in human fibroblasts. Oncogene 8: 1407–1413.
   PubMed Web of Science ®Google Scholar
• Spruck, C. H.III, M. Gonzalez-Zulueta, A. Shibata, A. R. Simoneau, M.-F. Lin, F. Gonzales, Y. Tsai, and P. A. Jones 1994. p16 gene in uncultured tumors. Science 370: 183–184.
   Google Scholar
• Stampfer, M. R. 1985. Isolation and growth of human mammary epithelial cells. J. Tissue Culture Methods 9: 107–115.
   Google Scholar
• Toth, M., U. Lichtenberg, and W. Doerfler 1989. Genomic sequencing reveals a 5-methylcytosine-free domain in active promoters and the spreading of preimposed methylation patterns. Proc. Natl. Acad. Sci. USA 86: 3728–3732.
   PubMedGoogle Scholar
• Wazer, D. E., X.-L. Liu, Q. Chu, Q. Gao, and V. Band 1995. Immortalization of distinct human mammary epithelial cell types by human papillomavirus 16 E6 or E7. Proc. Natl. Acad. Sci. USA 92: 3687–3691.
   PubMed Web of Science ®Google Scholar
• Weinberg, R. A. 1991. Tumor suppressor genes. Science 254: 1138–1146.
   PubMed Web of Science ®Google Scholar
• Werness, B. A., A. J. Levine, and P. M. Howley 1990. Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science 248: 76–79.
   PubMed Web of Science ®Google Scholar
• Wong, D. J., M. T. Barrett, R. Stoger, M. J. Emond, and B. J. Reid 1997. p16INK4A promoter is hypermethylated at a high frequency in esophageal adenocarcinomas. Cancer Res. 57: 2619–2622.
   PubMed Web of Science ®Google Scholar
• Xiong, Y., H. Zhang, and D. Beach 1993. Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation. Genes Dev. 7: 1572–1583.
   PubMed Web of Science ®Google Scholar
• Xu, L., D. Sgroi, C. J. Sterner, R. L. Beauchamp, D. M. Pinney, S. Keel, K. Ueki, J. L. Rutter, A. J. Buckler, D. N. Louis, J. F. Gusella, and V. Ramesh 1994. Mutational analysis of CDKN2 (MTS1/p16ink4) in human breast carcinomas. Cancer Res. 54: 5262–5264.
   PubMedGoogle Scholar
• Zerfass-Thome, K., W. Zwerschke, B. Mannhardt, R. Tindle, J. W. Botz, and P. Jansen-Durr 1996. Inactivation of the cdk inhibitor p27KIP1 by the human papillomavirus type 16 E7 oncoprotein. Oncogene 13: 2323–2330.
   PubMed Web of Science ®Google Scholar
• Zhang, L., X. Cui, K. Schmitt, R. Hubert, W. Navidi, and N. Arnheim 1992. Whole genome amplification from a single cell: implications for genetic analysis. Proc. Natl. Acad. Sci. USA 89: 5847–5851.
   PubMed Web of Science ®Google Scholar
• Zwijsen, R. M. L., R. Klompmaker, E. B. H. G. M. Weintjens, P. M. P. Kristel, B. van der Burg, and R. J. A. M. Michalides 1996. Cyclin D1 triggers autonomous growth of breast cancer cells by governing cell cycle exit. Mol. Cell. Biol. 16: 2554–2560.
   PubMed Web of Science ®Google Scholar