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Gene Expression

The β Subunit of Eukaryotic Translation Initiation Factor 2 Binds mRNA through the Lysine Repeats and a Region Comprising the C2-C2 Motif

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Pages 173-181 | Received 05 Aug 1998, Accepted 15 Sep 1998, Published online: 28 Mar 2023

REFERENCES

  • Bommer, J. A., R. Kraft, T. V. Kurzchalia, N. T. Price, and J. Proud 1991. Amino acid sequence analysis of the β and γ subunits of eucaryotic initiation factor eIF-2. Identification of regions interacting with GTP. Biochim. Biophys. Acta 1079:308–315.
  • Burd, C. G., and J. Dreyfuss 1994. Conserved structures and diversity of functions of RNA-binding proteins. Science 265:615–621.
  • Castilho-Valavicius, B., G. M. Thompson, and J. Donahue 1992. Mutation analysis of the Cys-X2-Cys-X19-Cys-X2-Cys motif on the β subunit of eukaryotic translation initiation factor 2. Gene Expr. 2:297–309.
  • Castilho-Valavicius, B., J. Yoon, and J. Donahue 1990. Genetic characterization of the Saccharomyces cerevisiae translational initiation suppressors sui1, sui2 and SUI3 and their effects on HIS4 expression. Genetics 124:483–495.
  • Chen, L., and J. Frankel 1994. An RNA-binding peptide from bovine immunodeficiency virus Tat protein recognizes an unsusual RNA structure. Biochemistry 33:2708–2715.
  • Cigan, A. M., J. L. Bushman, T. R. Boal, and J. Hinnebusch 1993. A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast. Proc. Natl. Acad. Sci. USA 90:5350–5354.
  • Cigan, A. M., M. Foiani, E. M. Hannig, and J. Hinnebusch 1991. Complex formation by positive and negative translational regulators of GCN4. Mol. Cell. Biol. 11:3217–3228.
  • Cigan, A. M., E. K. Pabich, and J. Donahue 1988. Mutational analysis of the HIS4 translational initiator region in Saccharomyces cerevisiae. Mol. Cell. Biol. 8:2964–2975.
  • Cigan, A. M., E. K. Pabich, L. Feng, and J. Donahue 1989. Yeast translation initiation suppressor sui2 encodes the α subunit of eukaryotic initiation factor 2 and shares sequence identity with the human α subunit. Proc. Natl. Acad. Sci. USA 86:2784–2788.
  • Das, S., T. Maiti, K. Das, and J. Maitra 1997. Specific interaction of eukaryotic translation initiation factor 5 (eIF5) with the β-subunit of eIF2. J. Biol. Chem. 272:31712–31718.
  • Donahue, T. F., A. M. Cigan, E. K. Pabich, and J. Castilho-Valavicius 1988. Mutations at a Zn(II) finger motif in the yeast eIF-2β gene alter ribosomal start-site selection during the scanning process. Cell 54:621–632.
  • Dorris, D. R., F. L. Erickson, and J. Hannig 1995. Mutations in GCD11, the structural gene for eIF-2γ in yeast, alter translational regulation of GCN4 and the selection of the start site for protein synthesis. EMBO J. 14:2239–2249.
  • Erickson, F. L., and J. Hannig 1996. Ligand interactions with eukaryotic translation initiation factor 2: role of the γ-subunit. EMBO J. 15:6311–6320.
  • Flynn, A., I. N. Shatsky, C. G. Proud, and J. Kaminski 1994. The RNA-binding properties of protein synthesis initiation factor eIF-2. Biochim. Biophys. Acta 1219:293–301.
  • Gonsky, R., D. Itamar, R. Harary, and J. Kaempfer 1992. Binding of ATP and messenger RNA by the β-subunit of eukaryotic initiation factor 2. Biochimie 74:427–434.
  • Guthrie C., G. R. Fink 1991. Guide to yeast genetics and molecular biology. Academic Press, Inc., New York, N.Y.
  • Hannig, E. M., A. M. Cigan, B. A. Freeman, and J. Kinzy 1993. GCD11, a negative regulator of GCN4 expression, encodes the γ subunit of eIF-2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 13:506–520.
  • Hemmerich, P., S. Bosbach, A. Von Mikecz, and J. Krawinkel 1997. Human ribosomal protein L7 binds RNA with an α-helical arginine-rich and lysine-rich domain. Eur. J. Biochem. 245:549–556.
  • Hershey, J. W. B. 1991. Translational control in mammalian cells. Annu. Rev. Biochem. 60:717–755.
  • Hill, J. E., A. M. Myers, T. J. Koerner, and J. Tzagoloff 1986. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2:163–167.
  • Hinnebush, A. G. 1993. Gene-specific translational control of the yeast GCN4 gene by phosphorylation of eukaryotic initiation factor 2. Mol. Microbiol. 10:215–223.
  • Huang, H., H. Yoon, E. M. Hannig, and J. Donahue 1997. GTP hydrolysis controls stringent selection of the AUG start codon during translation initation in Saccharomyces cerevisiae. Genes Dev. 11:2396–2413.
  • Laurino, J. P., and B. A. Castilho. Submitted for publication.
  • Lazinsky, D., E. Gradzielska, and J. Das 1989. Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine rich motif. Cell 59:207–218.
  • Merrick, W. C. 1992. Mechanism and regulation of eukaryotic protein synthesis. Microbiol. Rev. 56:291–315.
  • Methot, N., A. Pause, J. W. B. Hershey, and J. Sonenberg 1994. The translation initiation factor eIF-4B contains an RNA-binding region that is distinct and independent from its ribonucleoprotein consensus sequence. Mol. Cell. Biol. 14:2307–2316.
  • Mitchell, D. A., T. K. Marshall, and J. Deschenes 1993. Vectors for the inducible overexpression of glutathione-S-transferase fusion proteins in yeast. Yeast 9:715–723.
  • Pacheco, E., E. O. Melo, and B. A. Castilho. Unpublished observations.
  • Pathak, V. K., P. J. Nielsen, H. Trachsel, and J. Hershey 1988. Structure of the β subunit of translational initiation factor eIF-2. Cell 54:633–639.
  • Rhoads, R. E. 1993. Regulation of eukaryotic protein synthesis by initiation factors. J. Biol. Chem. 268:3017–3020.
  • Sikorsky, R. S., and J. Hieter 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:10–27.
  • Smith, D. B., and J. Johnson 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31–40.
  • Towbin, N., T. Staehelin, and J. Gordon 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354.
  • Ye, X., and J. Cavener 1994. Isolation and characterization of the Drosophila melanogaster gene encoding translation initiation factor eIF2β. Gene 142:271–274.

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