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DNA Dynamics and Chromosome Structure

The Chicken β-Globin 5′HS4 Boundary Element Blocks Enhancer-Mediated Suppression of Silencing

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Pages 3714-3726 | Received 22 Sep 1998, Accepted 16 Feb 1999, Published online: 28 Mar 2023

REFERENCES

  • Bender, M. A., A. Reik, J. Close, A. Telling, E. Epner, S. Fiering, R. Hardison, and J. Groudine 1998. Description and targeted deletion of 5′HS5 and 6 of the mouse β-globin locus control region. Blood 92:4394–4403.
  • Bouhassira, E. E., K. Westerman, and J. Leboulch 1997. Transcriptional behavior of LCR enhancer elements integrated at the same chromosomal locus by recombinase-mediated cassette exchange. Blood 90:3332–3344.
  • Cai, H., and J. Levine 1995. Modulation of enhancer-promoter interactions by insulators in the Drosophila embryo. Nature 376:533–536.
  • Cai, H. N., and J. Levine 1997. The gypsy insulator can function as a promoter-specific silencer in the Drosophila embryo. EMBO J 16:1732–1741.
  • Cavalli, G., and J. Paro 1998. The Drosophila Fab-7 chromosomal element conveys epigenetic inheritance during mitosis and meiosis. Cell 93:505–518.
  • Chan, C.-S., L. Rastelli, and J. Pirotta 1994. A Polycomb response element in the Ubx gene that determines an epigenetically inherited state of repression. EMBO J. 13:2553–2564.
  • Chung, J., M. Whitely, and J. Felsenfeld 1993. A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. Cell 74:505–514.
  • Chung, J. H., A. C. Bell, and J. Felsenfeld 1997. Characterization of the chicken β-globin insulator. Proc. Natl. Acad. Sci. USA 94:575–580.
  • Corces, V. G. 1995. Chromatin insulators: keeping enhancers under control. Nature 376:462–463.
  • Eissenberg, J. C., and J. Elgin 1991. Boundary functions in the control of gene expression. Trends Genet. 7:335–340.
  • Enver, T., Q. Li, K. Gale, M. Hu, G. May, J. Karlinsey, G. Jimenez, T. Papayannopoulou, and J. Costantini 1994. Analysis of the developmental and transcriptional potentiation functions of 5′HS2 of the murine β-globin locus control region in transgenic mice. Dev. Biol. 65:574–584.
  • Felsenfeld, G. 1996. Chromatin unfolds. Cell 86:13–19.
  • Felsenfeld, G., J. Boyes, J. Chung, D. Clark, and J. Studitsky 1996. Chromatin structure and gene expression. Proc. Natl. Acad. Sci. USA 93:9384–9388.
  • Festenstein, R., M. Tolaini, P. Corbella, C. Mamalaki, J. Parrington, M. Fox, A. Miliou, M. Jones, and J. Kiousses 1996. Locus control region function and heterochromatin-induced position effect variegation. Science 271:1123–1125.
  • Fiering, S. N., M. Roederer, G. P. Nolan, D. R. Micklem, D. R. Parks, and J. Herzenberg 1991. Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs. Cytometry 12:291–301.
  • Fukushige, S., and J. Sauer 1992. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 89:7905–7909.
  • Garrick, D., and E. Whitelaw. Unpublished data.
  • Gdula, D. A., and J. Corces 1997. Characterization of functional domains of the su(Hw) protein that mediate the silencing effect of mod(mdg4) mutations. Genetics 145:153–161.
  • Gdula, D. A., T. I. Gerasimova, and J. Corces 1996. Genetic and molecular analysis of the gypsy chromatin insulator of Drosophila. Proc. Natl. Acad. Sci. USA 93:9378–9383.
  • Gerasimova, T. I., D. A. Gdula, D. V. Gerasimov, O. Siimonova, and J. Corces 1995. A Drosophila protein that imparts directionality on a chromatin insulator is an enhancer of position-effect variegation. Cell 82:587–597.
  • Gerasimova, T. I., and J. Corces 1996. Boundary and insulator elements in chromosomes. Curr. Opin. Gene Dev. 6:185–192.
  • Gerasimova, T. I., and J. Corces 1998. Polycomb and trithorax group proteins mediate the function of a chromatin insulator. Cell 92:511–521.
  • Geyer, P. K. 1997. The role of insulator elements in defining domains of gene expression. Curr. Opin. Gene Dev. 7:242–248.
  • Hagstrom, K., M. Muller, and J. Schedl 1996. Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. Gene Dev. 10:3202–3215.
  • Hagstrom, K., M. Muller, and J. Schedl 1997. A Polycomb and GAGA dependent silencer adjoins the Fab-7 boundary in the Drosophila bithorax complex. Genetics 146:1365–1380.
  • Kellum, R., and J. Schedl 1991. A position-effect assay for boundaries of higher order chromosomal domains. Cell 64:941–950.
  • Kellum, R., and J. Schedl 1992. A group of scs elements function as domain boundaries in an enhancer-blocking assay. Mol. Cell. Biol. 12:2424–2431.
  • Mihaly, J., I. Hogga, J. Gausz, H. Gyurkovics, and J. Karch 1997. In situ dissection of the Fab-7 region of the bithroax complex into a chromatin domain boundary and a Polycomb-response element. Development 124:1809–1820.
  • Moon, A. M., and J. Ley 1991. Functional properties of the β-globin locus control region in K562 cells. Blood 77:2272–2284.
  • Nolan, G. P., S. Fiering, F. F. Nicolas, and J. Herzenberg 1988. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on β-d-galactosidase activity after transduction of Escherichia coli lacZ. Proc. Natl. Acad. Sci. USA 85:2603–2607.
  • Paro, R. 1995. Propagating memory of transcriptional states. Trends Genet. 11:295–297.
  • Pikaart, M. J., F. Recillas-Targa, and J. Felsenfeld 1998. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Genes Dev. 12:2852–2862.
  • Pirrotta, V., and J. Rastelli 1994. White gene expression, repressive chromatin domains and homeotic gene regulation in Drosophila. Bioessays 16:549–556.
  • Reik, A., A. Telling, G. Zitnik, E. Epner, and J. Groudine 1998. The locus control region is necessary for gene expression in the human β-globin locus but not the maintenance of an open chromatin structure in erythroid cells. Mol. Cell. Biol. 18:5992–6000.
  • Reitman, M., and J. Felsenfeld 1990. Developmental regulation of topoisomerase II sites and DNase I-hypersensitive sites in the chicken β-globin locus. Mol. Cell. Biol. 10:2774–2786.
  • Rivella, S., and J. Sadelain 1998. Genetic treatment of severe hemoglobinopathies: the combat against transgene variegation and transgene silencing. Semin. Hematol. 35:112–125.
  • Robertson, G., D. Garrick, W. Wu, M. Kearns, D. Martin, and J. Whitelaw 1995. Position-dependent variegation of globin transgene expression in mice. Proc. Natl. Acad. Sci. USA 92:5371–5375.
  • Sauer, B. 1993. Manipulation of transgenes by site-specific recombination: use of cre recombinase. Methods Enzymol. 225:890–900.
  • Schlake, T., and J. Bode 1994. Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 33:12746.
  • Sutherland, H. G. E., D. I. K. Martin, and J. Whitelaw 1997. A globin enhancer acts by increasing the proportion of erythrocytes expressing a linked transgene. Mol. Cell. Biol. 17:1607–1614.
  • Walters, M. C., W. Magis, S. Fiering, J. Eidemiller, D. Scalzo, M. Groudine, and J. Martin 1996. Transcriptional enhancers act in cis to suppress position-effect variegation. Genes Dev. 10:185–196.
  • Walters, M. C., S. Fiering, J. Eidemiller, W. Magis, M. Groudine, and J. Martin 1995. Enhancers increase the probability but not the level of gene expression. Proc. Natl. Acad. Sci. USA 92:7125–7129.
  • Wang, Y., F. J. DeMayo, S. Tsai, and J. O’Malley 1997. Ligand-inducible and liver-specific target gene expression in transgenic mice. Nat. Biotechnol. 15:239–243.
  • Weintraub, H. 1988. Formation of stable transcriptional complexes as assayed by analysis of individual templates. Proc. Natl. Acad. Sci. USA 92:5819–5823.
  • Westerman, K. A., and J. Leboulch 1996. Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc. Natl. Acad. Sci. USA 93:8971.
  • Zhong, X.-P., and J. Krangel 1997. An enhancer-blocking element between α and δ gene segments within the human T cell receptor α/δ locus. Proc. Natl. Acad. Sci. USA 94:5219–5224.
  • Zhou, J., S. Barolo, P. Szymanski, and J. Levine 1996. The Fab-7 element of the bithorax complex attenuates enhancer-promoter interactions in the Drosophila embryo. Genes Dev. 10:3195–3201.

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