Auto-Inhibition and Partner Proteins, Core-Binding Factor β (CBFβ) and Ets-1, Modulate DNA Binding by CBFα2 (AML1)

REFERENCES

• Adja, N., Stacy, T., Speck, N. A., and Liu, P. P.. 1998. The leukemic protein CBFβ-SMMHC sequesters CBFα2 into cytoskeletal filaments and aggregates. Mol. Cell. Biol. 18:7432–7443
   PubMedGoogle Scholar
• Albagli, O., Klaes, A., Ferreira, E., Leprince, D., and Klambt, C.. 1996. Function of ets genes is conserved between vertebrates and Drosophila. Mech. Dev. 59:29–40
   PubMed Web of Science ®Google Scholar
• Bae, S.-C., Ogawa, E., Maruyama, M., Oka, H., Satake, M., Shigesada, K., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., and Ito, Y.. 1994. PEBP2αB/mouse AML1 consists of multiple isoforms that possess differential transactivation potentials. Mol. Cell. Biol. 14:3242–3252
   PubMed Web of Science ®Google Scholar
• Bae, S.-C., Takahashi, E., Zhang, Y. W., Ogawa, E., Shigesada, K., Namba, Y., Satake, M., and Ito, Y.. 1995. Cloning, mapping and expression of PEBP2αC, a third gene encoding the mammalian Runt domain. Gene 159:245–248
   PubMed Web of Science ®Google Scholar
• Bae, S. C., Yamaguchi-Iwai, Y., Ogawa, E., Maruyama, M., Inuzuka, M., Kagoshima, H., Shigesada, K., Satake, M., and Ito, Y.. 1993. Isolation of PEBP2αB cDNA representing the mouse homolog of human acute myeloid leukemia gene, AML1. Oncogene 8:809–814
   PubMed Web of Science ®Google Scholar
• Barton, K., Muthusamy, N., Fischer, C., Ting, C. N., Walunas, T. L., Lanier, L. L., and Leiden, J. M.. 1998. The Ets-1 transcription factor is required for the development of natural killer cells in mice. Immunity 9:555–563
   PubMed Web of Science ®Google Scholar
• Ben-David, U., Giddens, E. B., Letwin, K., and Bernstein, A.. 1991. Erythroleukemia induction by Friend murine leukemia virus: insertional activation of a new member of the ets gene family, Fli-1, closely linked to c-ets-1. Genes Dev. 5:908–918
   PubMed Web of Science ®Google Scholar
• Berardi, M., Sun, C., Zehr, M., Abildgaard, F., Peng, J., Speck, N. A., and Bushweller, J. H.. 1999. The Ig fold of the core binding factor α Runt domain is a member of a family of structurally and functionally related Ig fold DNA binding domains. Structure 7:1247–1256
   PubMedGoogle Scholar
• Bories, J. C., Willerford, D. M., Grevin, D., Davidson, L., Camus, A., Martin, P., Stehelin, D., and Alt, F. W.. 1995. Increased T-cell apoptosis and terminal B-cell differentiation induced by inactivation of the Ets-1 proto-oncogene. Nature 377:635–638
   PubMed Web of Science ®Google Scholar
• Brass, A. L., Kehrli, E., Eisenbeis, C. F., Storb, U., and Singh, H.. 1996. Pip, a lymphoid-restricted IRF, contains a regulatory domain that is important for autoinhibition and ternary complex formation with the ets factor PU.1. Genes Dev. 10:2335–2347
   PubMed Web of Science ®Google Scholar
• Brunner, D., Ducker, K., Oellers, N., Hafen, E., Scholz, H., and Klambt, C.. 1994. The ETS domain protein pointed-P2 is a target of MAP kinase in the sevenless signal transduction pathway. Nature 370:386–389
   PubMed Web of Science ®Google Scholar
• Crute, B. E., Lewis, A. F., Wu, Z., Bushweller, J. H., and Speck, N. A.. 1996. Biochemical and biophysical properties of the CBFα2 (AML1) DNA-binding domain. J. Biol. Chem. 271:26251–26260
   PubMedGoogle Scholar
• Daga, A., Karlovich, C. A., Dumstrei, K., and Banerjee, U.. 1996. Patterning of cells in the Drosophila eye by Lozenge, which shares homologous domains with AML1. Genes Dev. 10:1194–1205
   PubMedGoogle Scholar
• Dalton, S., and Treisman, R.. 1992. Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element. Cell 68:597–612
   PubMed Web of Science ®Google Scholar
• Dignam, J. D., Lebowitz, R. M., and Roeder, R. G.. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475–1489
   PubMed Web of Science ®Google Scholar
• Duffy, J. B., and Gergen, J. P.. 1991. The Drosophila segmentation gene runt acts as a position-specific numerator element necessary for the uniform expression of the sex-determining gene Sex-lethal. Genes Dev. 5:2176–2187
   PubMed Web of Science ®Google Scholar
• Duffy, J. B., Kania, M. A., and Gergen, J. P.. 1991. Expression and function of the Drosophila gene runt in early stages of neural development. Development 113:1223–1230
   PubMedGoogle Scholar
• Erman, B., Cortes, M., Speck, N. A., and Sen, R.. 1998. ETS-core binding factor: a common composite motif in antigen receptor gene enhancers. Mol. Cell. Biol. 18:1322–1330
   PubMedGoogle Scholar
• Giese, K., Kingsley, C., Kirshner, J. R., and Grosschedl, R.. 1995. Assembly and function of a TCRα enhancer complex is dependent on LEF-1-induced DNA binding and multiple protein-protein interactions. Genes Dev. 9:995–1008
   PubMed Web of Science ®Google Scholar
• Goetz, T. L., Gu, T. L., Speck, N. A., and Graves, B. J.. 1999. Auto-inhibition of Ets-1 is counteracted by DNA binding cooperativity with core-binding factor α2. Mol. Cell. Biol. 20:81–90
   Google Scholar
• Goger, M., Gupta, V., Kim, W.-Y., Shigesada, K., Ito, Y., and Werner, M. H.. 1999. Molecular insights into PEBP2/CBFβ-SMMHC associated acute leukemia revealed from the three-dimensional structure of PEBP2/CBFβ. Nat. Struct. Biol. 6:620–623
   PubMedGoogle Scholar
• Golling, G., Li, L.-H., Pepling, M., Stebbins, M., and Gergen, J. P.. 1996. Drosophila homologues of proto-oncogene product PEBP2/CBFβ regulate the DNA-binding properties of Runt. Mol. Cell. Biol. 16:932–942
   PubMed Web of Science ®Google Scholar
• Golub, T. R., Barker, G. F., Bohlander, S. K., Hiebert, S., Ward, D. C., Bray-Ward, P., Morgan, E., Raimondi, S. C., Rowley, J. D., and Gilliland, D. G.. 1995. Fusion of the TEL gene on 12p13 to the AML1 gene on 21q22 in acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 92:4917–4921
   PubMed Web of Science ®Google Scholar
• Graves, B. J., Cowley, D. O., Goetz, T. L., Petersen, J. M., Jonsen, M. D., and Gillespie, M. E.. 1998. Autoinhibition as a transcriptional regulatory mechanism. Cold Spring Harbor Symp. Quant. Biol. 63:621–629
   PubMedGoogle Scholar
• Graves, B. J., and Peterson, J. M.. 1998. Specificity within the ets family of transcription factors. Adv. Cancer Res. 75:1–55
   PubMed Web of Science ®Google Scholar
• Huang, X., Crute, B. E., Sun, C., Tang, Y.-Y., Kelley, J. J.III, Lewis, A. F., Hartman, K. L., Laue, T. M., Speck, N. A., and Bushweller, J. H.. 1998. Overexpression, purification, and biophysical characterization of the heterodimerization domain of the core-binding factor β subunit. J. Biol. Chem. 273:2480–2487
   PubMedGoogle Scholar
• Huang, X., Peng, J. W., Speck, N. A., and Bushweller, J. H.. 1999. Core binding factor β revealed: solution structure and map of the CBFα binding site. Nat. Struct. Biol. 6:624–627
   PubMedGoogle Scholar
• Ingham, P., and Gergen, P.. 1988. Interactions between the pair-rule genes runt, hairy, even-skipped and fushi tarazu and the establishment of periodic pattern in the Drosophila embryo. Development 104 (Suppl.):51–60
   PubMedGoogle Scholar
• Jonsen, M. D., Petersen, J. M., Xu, Q.-P., and Graves, B. J.. 1996. Characterization of cooperative function of inhibitory sequences in Ets-1. Mol. Cell. Biol. 16:2065–2073
   PubMed Web of Science ®Google Scholar
• Kagoshima, H., Akamatsu, Y., Ito, Y., and Shigesada, K.. 1996. Functional dissection of the α and β subunits of the transcription factor PEBP2 and the redox susceptibility of its DNA binding activity. J. Biol. Chem. 271:33074–33082
   PubMedGoogle Scholar
• Kagoshima, H., Shigesada, K., Satake, M., Ito, Y., Miyoshi, H., Ohki, M., Pepling, M., and Gergen, J. P.. 1993. The Runt-domain identifies a new family of heteromeric DNA-binding transcriptional regulatory proteins. Trends Genet. 9:338–341
   PubMedGoogle Scholar
• Kanno, T., Kanno, Y., Chen, L.-F., Ogawa, E., Kim, W.-Y., and Ito, Y.. 1998. Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/core binding factor α subunit revealed in the presence of the β subunit. Mol. Cell. Biol. 18:2444–2454
   PubMedGoogle Scholar
• Kim, W.-Y., Sieweke, M., Ogawa, E., Wee, H.-J., Englmeier, U., Graf, T., and Ito, Y.. 1999. Mutual activation of Ets-1 and AML1 DNA binding by direct interaction of their autoinhibitory domains. EMBO J. 18:1609–1620
   PubMed Web of Science ®Google Scholar
• Komori, T., Yagi, H., Nomura, S., Yamaguchi, A., Sasaki, K., Deguchi, K., Shimizu, Y., Bronson, R. T., Gao, Y.-H., Inada, M., Sato, M., Okamoto, R., Kitamura, Y., Yoshiki, S., and Kishimoto, T.. 1997. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89:755–764
   PubMed Web of Science ®Google Scholar
• Leprince, D., Gegonne, A., Coll, J., de Taisne, C., Schneeberger, A., Lagrou, C., and Stehelin, D.. 1983. A putative second cell-derived oncogene of the avian leukaemia retrovirus E26. Nature 306:395–397
   PubMed Web of Science ®Google Scholar
• Levanon, D., Negreanu, V., Bernstein, Y., Bar-Am, I., Avivi, L., and Groner, Y.. 1994. AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization. Genomics 23:425–432
   PubMed Web of Science ®Google Scholar
• Li, L. H., and Gergen, J. P.. 1999. Differential interactions between Brother proteins and Runt domain proteins in the Drosophila embryo and eye. Development 126:3313–3322
   PubMedGoogle Scholar
• Ling, Y., Lakey, J. H., Roberts, C. E., and Sharrocks, A. D.. 1997. Molecular characterization of the B-box protein-protein interaction motif of the ETS-domain transcription factor Elk-1. EMBO J. 16:2431–2440
   PubMedGoogle Scholar
• Liu, P., Tarle, S. A., Hajra, A., Claxton, D. F., Marlton, P., Freedman, M., Siciliano, M. J., and Collins, F. S.. 1993. Fusion between transcription factor CBFβ/PEBP2β and a myosin heavy chain in acute myeloid leukemia. Science 261:1041–1044
   PubMed Web of Science ®Google Scholar
• Lu, J., Maruyama, M., Satake, M., Bae, S.-C., Ogawa, E., Kagoshima, H., Shigesada, K., and Ito, Y.. 1995. Subcellular localization of the α and β subunits of acute myeloid leukemia-linked transcription factor PEBP2/CBF. Mol. Cell. Biol. 15:1651–1661
   PubMed Web of Science ®Google Scholar
• Mayall, T. P., Sheridan, P. L., Montminy, M. R., and Jones, K. A.. 1997. Distinct roles for P-CREB and LEF-1 in TCR enhancer assembly and activation on chromatin templates in vitro. Genes Dev. 11:887–899
   PubMed Web of Science ®Google Scholar
• McKercher, S. R., Torbett, B. E., Anderson, K. L., Henkel, G. W., Vestal, D. J., Baribault, H., Klemsz, M., Feeney, A. J., Wu, G. E., Paige, C. J., and Maki, R. A.. 1996. Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities. EMBO J. 15:5647–5658
   PubMed Web of Science ®Google Scholar
• McLean, T. W., Ringold, S., Neuberg, D., Stegmaier, K., Tantravahi, R., Ritz, J., Koeffler, H. P., Takeuchi, S., Janssen, J. W., Seriu, T., Bartram, C. R., Sallan, S. E., Gilliland, D. G., and Golub, T. R.. 1996. TEL/AML-1 dimerizes and is associated with a favorable outcome in childhood acute lymphoblastic leukemia. Blood 88:4252–4258
   PubMed Web of Science ®Google Scholar
• Melet, F., Motro, B., Rossi, D. J., Zhang, L., and Bernstein, A.. 1996. Generation of a novel Fli-1 protein by gene targeting leads to a defect in thymus development and a delay in Friend virus-induced erythroleukemia. Mol. Cell. Biol. 16:2708–2718
   PubMed Web of Science ®Google Scholar
• Meyers, S., Downing, J. R., and Hiebert, S. W.. 1993. Identification of AML-1 and the (8;21) translocation protein (AML-1/ETO) as sequence-specific DNA-binding proteins: the runt homology domain is required for DNA binding and protein-protein interactions. Mol. Cell. Biol. 13:6336–6345
   PubMed Web of Science ®Google Scholar
• Meyers, S., Lenny, N., and Hiebert, S. W.. 1995. The t(8;21) fusion protein interferes with AML1-1B-dependent transcriptional activation. Mol. Cell. Biol. 15:1974–1982
   PubMed Web of Science ®Google Scholar
• Miyoshi, H., Ohira, M., Shimizu, K., Mitani, K., Hirai, H., Imai, T., Yokoyama, K., Soeda, E., and Ohki, M.. 1995. Alternative splicing and genomic structure of the AML1 gene involved in acute myeloid leukemia. Nucleic Acids Res. 23:2762–2769
   PubMed Web of Science ®Google Scholar
• Miyoshi, H., Shimizu, K., Kozu, T., Maseki, N., Kaneko, Y., and Ohki, M.. 1991. t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1. Proc. Natl. Acad. Sci. USA 88:10431–10434
   PubMed Web of Science ®Google Scholar
• Moreau-Gachelin, F., Tavitian, A., and Tambourin, P.. 1988. Spi-1 is a putative oncogene in virally induced murine erythroleukaemias. Nature 331:277–280
   PubMed Web of Science ®Google Scholar
• Muthusamy, N., Barton, K., and Leiden, J. M.. 1995. Defective activation and survival of T cells lacking the Ets-1 transcription factor. Nature 377:639–642
   PubMed Web of Science ®Google Scholar
• Nagata, T., Gupta, V., Sorce, D., Kim, W.-Y., Sali, A., Chait, B. T., Shigesada, K., Ito, Y., and Werner, M. H.. 1999. Immunoglobulin motif DNA-binding and heterodimerization for the PEBP2/CBF Runt-domain. Nat. Struct. Biol. 6:615–619
   PubMedGoogle Scholar
• Niki, M., Okada, H., Takano, H., Kuno, J., Tani, K., Hibino, H., Asano, S., Ito, Y., Satake, M., and Noda, T.. 1997. Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcription factor, polyomavirus enhancer binding protein 2/core binding factor. Proc. Natl. Acad. Sci. USA 94:5697–5702
   PubMedGoogle Scholar
• North, T. E., Gu, T.-L., Stacy, T., Wang, Q., Howard, L., Binder, M., Marín-Padilla, M., and Speck, N. A.. 1999. Cbfa2 is required for the formation of intra-aortic hematopoietic clusters. Development 126:2563–2575
   PubMed Web of Science ®Google Scholar
• Nunn, M. F., Seeberg, P. H., Moscovici, C., and Duesberg, P. H.. 1983. Tripartite structure of the avian erythroblastosis virus E26 transforming gene. Nature 306:391–395
   PubMed Web of Science ®Google Scholar
• Nye, J. A., Petersen, J. M., Gunther, C. V., Jonsen, M. D., and Graves, B. J.. 1992. Interaction of murine Ets-1 with GGA-binding sites establishes the ETS domain as a new DNA-binding motif. Genes Dev. 6:975–990
   PubMed Web of Science ®Google Scholar
• Ogawa, E., Inuzuka, M., Maruyama, M., Satake, M., Naito-Fujimoto, M., Ito, Y., and Shigesada, K.. 1993. Molecular cloning and characterization of PEBP2β, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2α. Virology 194:314–331
   PubMed Web of Science ®Google Scholar
• Ogawa, E., Maruyama, M., Kagoshima, H., Inuzuka, M., Lu, J., Satake, M., Shigesada, K., and Ito, Y.. 1993. PEBP2/PEA2 represents a new family of transcription factor homologous to the products of the Drosophila runt and the human AML1 gene. Proc. Natl. Acad. Sci. USA 90:6859–6863
   PubMed Web of Science ®Google Scholar
• Okuda, T., van Deursen, J., Hiebert, S. W., Grosveld, G., and Downing, J. R.. 1996. AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis. Cell 84:321–330
   PubMed Web of Science ®Google Scholar
• O'Neill, E. M., Rebay, I., Tjian, R., and Rubin, G. M.. 1994. The activities of two Ets-related transcription factors required for Drosophila eye development are modulated by the Ras/MAPK pathway. Cell 78:137–147
   PubMed Web of Science ®Google Scholar
• Otto, F., Thornell, A. P., Crompton, T., Denzel, A., Gilmour, K. C., Rosewell, I. R., Stamp, G. W. H., Beddington, R. S. P., Mundlos, S., Olsen, B. R., Selby, P. B., and Owen, M. J.. 1997. Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell 89:765–772
   PubMed Web of Science ®Google Scholar
• Petersen, J. M., Skalicky, J. J., Donaldson, L. W., McIntosh, L. P., Alber, T., and Graves, B. J.. 1995. Modulation of transcription factor Ets-1 DNA binding: DNA-induced unfolding of an alpha helix. Science 269:1866–1869
   PubMed Web of Science ®Google Scholar
• Petrovick, M. S., Hiebert, S. W., Friedman, A. D., Hetherington, C. J., Tenen, T. G., and Zhang, D.-E.. 1998. Multiple functional domains of AML1: PU.1 and C/EBPα synergize with different regions of AML1. Mol. Cell. Biol. 18:3915–3925
   PubMedGoogle Scholar
• Rabbitts, T. H.. 1994. Chromosomal translocations in human cancer. Nature 372:143–149
   PubMed Web of Science ®Google Scholar
• Rizki, T. M., Rizki, R. M., and Bellotti, R.. 1985. Genetics of a Drosophila phenoloxidase. Mol. Gen. Genet. 201:7–13
   PubMedGoogle Scholar
• Romana, S. P., Mauchauffe, M., Le Coniat, M., Chumakow, I., Le Paslier, D., Berger, R., and Bernard, O. A.. 1995. The t(12;21) of acute lymphoblastic leukemia results in a tel-AML1 gene fusion. Blood 85:3662–3670
   PubMed Web of Science ®Google Scholar
• Romana, S. P., Poirel, H., Leconiat, M., Flexor, M.-A., Mauchauffé, M., Jonveaux, P., Macintyre, E. A., Berger, R., and Bernard, O. A.. 1995. High frequency of t(12;21) in childhood B-lineage acute lymphoblastic leukemia. Blood 86:4263–4269
   PubMed Web of Science ®Google Scholar
• Sanchez, L., and Nothiger, R.. 1983. Sex determination and dosage compensation in Drosophila melanogaster: production of male clones in XX females. EMBO J. 2:485–491
   PubMed Web of Science ®Google Scholar
• Sasaki, K., Yagi, H., Bronson, R. T., Tominaga, K., Matsunashi, T., Deguchi, K., Tani, Y., Kishimoto, T., and Komori, T.. 1996. Absence of fetal liver hematopoiesis in transcriptional co-activator, core binding factor β (Cbfb) deficient mice. Proc. Natl. Acad. Sci. USA 93:12359–12363
   PubMed Web of Science ®Google Scholar
• Sato, M., Morii, E., Komori, T., Kawahata, H., Sugimoto, M., Terai, K., Shimizu, H., Yasui, Y., Ogihara, H., Yasui, N., Ochi, T., Kitamura, Y., Ito, Y., and Nomura, S.. 1998. Transcriptional regulation of osteopontin gene in vivo by PEBP2αA/CBFA1 and ETS1 in the skeletal tissues. Oncogene 17:1517–1525
   PubMed Web of Science ®Google Scholar
• Scott, E. W., Simon, M. C., Anastasi, J., and Singh, H.. 1994. Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages. Science 265:1573–1577
   PubMed Web of Science ®Google Scholar
• Sharrocks, A. D., Brown, A. L., Ling, Y., and Yates, P. R.. 1998. The Ets-domain transcription factor family. Internat. J. Biochem. 29:1371–1387
   Google Scholar
• Shurtleff, S. A., Buijs, A., Behm, F. G., Rubnitz, J. E., Raimondi, S. C., Hancock, M. L., Chan, G. C.-F., Pui, C.-H., Grosveld, G., and Downing, J. R.. 1995. TEL/AML1 fusion resulting from a cryptic t(12;21) is the most common genetic lesion in pediatric ALL and defines a subgroup of patients with an excellent prognosis. Leukemia 9:1985–1989
   PubMed Web of Science ®Google Scholar
• Skalicky, J. J., Donaldson, L. W., Petersen, J. M., Graves, B. J., and McIntosh, L. P.. 1996. Structural coupling of the inhibitory regions flanking the ETS domain of murine Ets-1. Protein Sci. 5:296–309
   PubMedGoogle Scholar
• Speck, N. A., and Baltimore, D.. 1987. Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer. Mol. Cell. Biol. 7:1101–1110
   PubMedGoogle Scholar
• Speck, N. A., and Stacy, T.. 1995. A new transcription factor family associated with human leukemias. Crit. Rev. Eukaryotic Gene Expr. 5:337–364
   PubMed Web of Science ®Google Scholar
• Stewart, M., Terry, A., Hu, M., O'Hara, M., Blyth, K., Baxter, E., Cameron, E., Onions, D. E., and Neil, J. C.. 1997. Proviral insertions induce the expression of bone-specific isoforms of PEBP2αA (CBFA1): evidence for a new myc collaborating oncogene. Proc. Natl. Acad. Sci. USA 94:8646–8651
   PubMed Web of Science ®Google Scholar
• Stocker, F. R., and Gendre, N.. 1988. Peripheral and central nervous system effects of lz, a Drosophila mutant lacking basiconic antennal sensilla. Dev. Biol. 127:12–27
   PubMedGoogle Scholar
• Sun, W., Graves, B. J., and Speck, N. A.. 1995. Transactivation of the Moloney murine leukemia virus and T-cell receptor β-chain enhancers by cbf and ets requires intact binding sites for both proteins. J. Virol. 69:4941–4949
   PubMed Web of Science ®Google Scholar
• Tanaka, K., Tanaka, T., Kurokawa, M., Imai, Y., Ogawa, S., Mitani, K., Yazaki, Y., and Hirai, H.. 1998. The AML/ETO(MTG8) and AML1/Evi-1 leukemia-associated chimeric oncoproteins accumulate PEBP2β(CBFβ) in the nucleus more efficiently than wild-type AML1. Blood 91:1688–1699
   PubMed Web of Science ®Google Scholar
• Thirunavukkarasu, K., Mahajan, M., McLarren, K. W., Stifani, S., and Karsenty, G.. 1998. Two domains unique to osteoblast-specific transcription factor Osf2/Cbfa1 contribute to its transactivation function and its inability to heterodimerize with Cbfβ. Mol. Cell. Biol. 18:4197–4208
   PubMed Web of Science ®Google Scholar
• Thornell, A., Hallberg, B., and Grundstrom, T.. 1991. Binding of SL3-3 enhancer factor 1 transcriptional activators to viral and chromosomal enhancer sequences. J. Virol. 65:42–50
   PubMedGoogle Scholar
• Wang, Q., Stacy, T., Binder, M., Marín-Padilla, M., Sharpe, A. H., and Speck, N. A.. 1996. Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. Proc. Natl. Acad. Sci. USA 93:3444–3449
   PubMed Web of Science ®Google Scholar
• Wang, Q., Stacy, T., Miller, J. D., Lewis, A. F., Huang, X., Bories, J.-C., Bushweller, J. H., Alt, F. W., Binder, M., Marín-Padilla, M., Sharpe, A., and Speck, N. A.. 1996. The CBFβ subunit is essential for CBFα2 (AML1) function in vivo. Cell 87:697–708
   PubMed Web of Science ®Google Scholar
• Wang, S., and Speck, N. A.. 1992. Purification of core-binding factor, a protein that binds the conserved core site in murine leukemia virus enhancers. Mol. Cell. Biol. 12:89–102
   PubMedGoogle Scholar
• Wang, S., Wang, Q., Crute, B. E., Melnikova, I. N., Keller, S. R., and Speck, N. A.. 1993. Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. Mol. Cell. Biol. 13:3324–3339
   PubMed Web of Science ®Google Scholar
• Wotton, D., Ghysdael, J., Wang, S., Speck, N. A., and Owen, M. J.. 1994. Cooperative binding of Ets-1 and core binding factor to DNA. Mol. Cell. Biol. 14:840–850
   PubMed Web of Science ®Google Scholar
• Zeng, C., Van Wignen, A. J., Stein, J. L., Meyers, S., Sun, W., Shopland, L., Lawrence, J. B., Penman, S., Lian, J. B., Stein, G. S., and Hiebert, S. W.. 1997. Identification of a nuclear matrix targeting signal in the leukemia and bone-related AML/CBF-α transcription factors. Proc. Natl. Acad. Sci. USA 94:6746–6751
   PubMedGoogle Scholar