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DNA Dynamics and Chromosome Structure

Schizosaccharomyces pombe Cells Lacking the Amino-Terminal Catalytic Domains of DNA Polymerase Epsilon Are Viable but Require the DNA Damage Checkpoint Control

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Pages 4495-4504 | Received 14 Feb 2001, Accepted 30 Apr 2001, Published online: 28 Mar 2023

REFERENCES

  • al-Khodairy, F., E. Fotou, K. S. Sheldrick, D. J. Griffiths, A. R. Lehmann, and A. M. Carr. 1994. Identification and characterization of new elements involved in checkpoint and feedback controls in fission yeast. Mol. Biol. Cell 5:147–160.
  • al-Khodairy, F., and A. M. Carr. 1992. DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe. EMBO J. 11:1343–1350.
  • Aparicio, O. M., D. M. Weinstein, and S. P. Bell. 1997. Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91:59–69.
  • Araki, H., R. K. Hamatake, L. H. Johnston, and A. Sugino. 1991. DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 88:4601–4605.
  • Araki, H., R. K. Hamatake, A. Morrison, A. L. Johnson, L. H. Johnston, and A. Sugino. 1991. Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae. Nucleic Acids Res. 19:4867–4872.
  • Araki, H., S. H. Leem, A. Phongdara, and A. Sugino. 1995. Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Proc. Natl. Acad. Sci. USA 92:11791–11795.
  • Araki, H., P. A. Ropp, A. L. Johnson, L. H. Johnston, A. Morrison, and A. Sugino. 1992. DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae. EMBO J. 11:733–740.
  • Boddy, M. N., B. Furnari, O. Mondesert, and P. Russell. 1998. Replication checkpoint enforced by kinases Cds1 and Chk1. Science 280:909–912.
  • Budd, M. E., and J. L. Campbell. 1993. DNA polymerases delta and epsilon are required for chromosomal replication in Saccharomyces cerevisiae. Mol. Cell. Biol. 13:496–505.
  • Caspari, T., and A. M. Carr. 1999. DNA structure checkpoint pathways in Schizosaccharomyces pombe. Biochimie 81:173–181.
  • Dua, R., D. L. Levy, and J. L. Campbell. 1998. Role of the putative zinc finger domain of Saccharomyces cerevisiae DNA polymerase ɛ in DNA replication and the S/M checkpoint pathway. J. Biol. Chem. 273:30046–30055.
  • Dua, R., D. L. Levy, and J. L. Campbell. 1999. Analysis of the essential functions of the C-terminal protein/protein interaction domain of Saccharomyces cerevisiae pol epsilon and its unexpected ability to support growth in the absence of the DNA polymerase domain. J. Biol. Chem. 274:22283–22288.
  • D'Urso, G., and P. Nurse. 1995. Checkpoints in the cell cycle of fission yeast. Curr. Opin. Genet. Dev. 5:12–16.
  • D'Urso, G., and P. Nurse. 1997. Schizosaccharomyces pombe cdc20+ encodes DNA polymerase epsilon and is required for chromosomal replication but not for the S phase checkpoint. Proc. Natl. Acad. Sci. USA 94:12491–12496.
  • Hamatake, R. K., H. Hasegawa, A. B. Clark, K. Bebenek, T. A. Kunkel, and A. Sugino. 1990. Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. J. Biol. Chem. 265:4072–4083.
  • Hartwell, L., and T. Weinert. 1989. Checkpoints: controls that ensure the order of cell cycle events. Science 246:629–634.
  • Holmes, A. M., and J. E. Haber. 1999. Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases. Cell 96:415–424.
  • Jessberger, R., V. Podust, U. Hubscher, and P. Berg. 1993. A mammalian protein complex that repairs double-strand breaks and deletions by recombination. J. Biol. Chem. 268:15070–15079.
  • Kamimura, Y., H. Masumoto, A. Sugino, and H. Araki. 1998. Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol. Cell. Biol. 18:6102–6109.
  • Keeney, J. B., and J. D. Boeke. 1994. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136:849–856.
  • Kesti, T., H. Frantti, and J. E. Syvaoja. 1993. Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase epsilon. J. Biol. Chem. 268:10238–10245.
  • Kesti, T., K. Flick, S. Keranen, J. E. Syvaoja, and C. Wittenberg. 1999. DNA polymerase epsilon catalytic domains are dispensable for DNA replication, DNA repair, and cell viability. Mol. Cell 3:679–685.
  • Li, Y., H. Asahara, V. S. Patel, S. Zhou, and S. Linn. 1997. Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. J. Biol. Chem. 272:32337–32344.
  • Li, Y, Z. F. Pursell, and S. Linn. 2000. Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon. J. Biol. Chem. 275:23247–23552.
  • Lindsay, H. D., D. J. Griffiths, R. J. Edwards, P. U. Christensen, J. M. Murray, F. Osman, N. Walworth, and A. M. Carr. 1998. S-phase-specific activation of Cds1 kinase defines a subpathway of checkpoint response in Schizosaccharomyces pombe.. Genes Dev. 12:382–395.
  • Liu, V. F., D. Bhaumik, and T. S. Wang. 1999. Mutator phenotype induced by aberrant replication. Mol. Cell. Biol. 19:1126–1135.
  • Masumoto, H., A. Sugino, and H. Araki. 2000. Dpb11 controls the association between DNA polymerases alpha and epsilon and the autonomously replicating sequence region of budding yeast. Mol. Cell. Biol. 20:2809–2817.
  • Maundrell, K.. 1993. Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123:127–130.
  • McFarlane, R. J., A. M. Carr, and C. Price. 1997. Characterisation of the Schizosaccharomyces pombe rad4/cut5 mutant phenotypes: dissection of DNA replication and G2 checkpoint control function. Mol. Gen. Genet. 255:332–340.
  • Mimura, M., T. Masuda, T. Matsui, and H. Takisawa. 2000. Central role for Cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts. Genes Cells 5:469–452.
  • Moreno, S., A. Klar, and P. Nurse. 1991. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194:795–823.
  • Morrison, A., H. Araki, A. B. Clark, R. K. Hamatake, and A. Sugino. 1990. A third essential DNA polymerase in S. cerevisiae. Cell 62:1143–1151.
  • Murakami, H., and P. Nurse. 2000. DNA replication and damage checkpoints and meiotic cell cycle controls in the fission and budding yeasts. Biochem. J. 349:1–12.
  • Nurse, P., P. Thuriaux, and K. Nasmyth. 1976. Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol. Gen. Genet. 146:167–178.
  • Reid, R. J. D., P. Fiorani, M. Sugawara, and M. Bjornsti. 1999. CDC45 and DPB11 are required for processive DNA replication and resistance to DNA topoisomerase I-mediated DNA damage. Proc. Natl. Acad. Sci. USA 96:11440–11445.
  • Rhind, N., and P. Russell. 1998. The Schizosaccharomyces pombe S-phase checkpoint differentiates between different types of DNA damage. Genetics 149:1729–1737.
  • Saka, Y., P. Fantes, T. Sutani, C. McInerny, J. Creanor, and M. Yanagida. 1994. Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. EMBO J. 13:5319–5329.
  • Saka, Y., and M. Yanagida. 1993. Fission yeast cut5+, required for S phase onset and M phase restraint, is identical to the radiation-damage repair gene rad4+. Cell 74:383–393.
  • Shivji, M. K., V. N. Podust, U. Hübscher, and R. D. Wood. 1995. Nucleotide excision repair DNA synthesis by DNA polymerase epsilon in the presence of PCNA, RFC, and RPA. Biochemistry 34:5011–5017.
  • Sugino, A., T. Ohara, J. Sebastian, N. Nakashima, and H. Araki. 1998. DNA polymerase epsilon encoded by cdc20+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe. Genes Cells 3:99–110.
  • Verkade, H. M., and M. J. O'Connell. 1998. Cut5 is a component of the UV-responsive DNA damage checkpoint in fission yeast. Mol. Gen. Genet. 260:426–433.
  • Walworth, N., S. Davey, and D. Beach. 1993. Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2. Nature 363:368–371.
  • Wang, H., and S. J. Elledge. 1999. DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90:3824–3829.
  • Wang, Z., X. Wu, and E. C. Freidberg. 1993. DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae. Mol. Cell. Biol. 13:1051–1058.
  • Weinert, T.. 1998. DNA damage and checkpoint pathways: molecular anatomy and interactions with repair. Cell 94:555–558.
  • Zou, L., and B. Stillman. 1998. Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280:593–596.

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