Two Immunologically Distinct Human DNA Polymerase α-Primase Subpopulations Are Involved in Cellular DNA Replication

REFERENCES

• Aparicio, O. M., A. M. Stout, and S. P. Bell. 1999. Differential assembly of Cdc45p and DNA polymerases at early and late origins of DNA replication. Proc. Natl. Acad. Sci. USA 96:9130–9135.
   PubMed Web of Science ®Google Scholar
• Aparicio, O. M., D. M. Weinstein, and S. P. Bell. 1997. Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91:59–69.
   PubMed Web of Science ®Google Scholar
• Cegielska, A., S. Shaffer, R. Derua, J. Goris, and D. M. Virshup. 1994. Different oligomeric forms of protein phosphatase 2A activate and inhibit simian virus 40 DNA replication. Mol. Cell. Biol. 14:4616–4623.
   PubMedGoogle Scholar
• Collins, K. L., A. A. R. Russo, B. Y. Tseng, and T. J. Kelly. 1993. The role of the 70 kDa subunit of human DNA polymerase α in DNA replication. EMBO J. 12:4555–4566.
   PubMedGoogle Scholar
• Diffley, J. F. X.. 1996. Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells. Genes Dev. 10:2819–2830.
   PubMedGoogle Scholar
• Dimitrova, D. S., I. T. Todorov, T. Melendy, and D. M. Gilbert. 1999. Mcm2, but not RPA, is a component of the mammalian early G1-phase prereplication complex. J. Cell Biol. 146:709–722.
   PubMed Web of Science ®Google Scholar
• Dornreiter, I., A. Höβ, A. K. Arthur, and E. Fanning. 1990. SV40 T antigen binds directly to the catalytic subunit of DNA polymerase α. EMBO J. 9:3329–3336.
   PubMedGoogle Scholar
• Dornreiter, I., F. L. Erdile, I. Gilbert, D. von Winkler, T. J. Kelly, and E. Fanning. 1992. Interaction of DNA polymerase α-primase with replication protein A and SV40 T antigen. EMBO J. 11:769–776.
   PubMed Web of Science ®Google Scholar
• Dornreiter, I., W. C. Copeland, and T. S.-F. Wang. 1993. Initiation of simian virus 40 DNA replication requires the interaction of a specific domain of human DNA polymerase α with large T antigen. Mol. Cell. Biol. 13:809–820.
   PubMedGoogle Scholar
• Dutta, A., and S. P. Bell. 1997. Initiation of DNA replication in eukaryotic cells. Annu. Rev. Cell. Dev. Biol. 13:293–332.
   PubMed Web of Science ®Google Scholar
• Foiani, M., G. Liberi, G. Lucchini, and P. Plevani. 1995. Cell cycle-dependent phosphorylation and dephosphorylation of the yeast DNA polymerase α-primase B subunit. Mol. Cell. Biol. 15:883–891.
   PubMedGoogle Scholar
• Gurney, E. G., R. O. Harrison, and J. Fenno. 1980. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct subclasses of large T antigen and for similarities among nonviral T antigens. J. Virol. 34:752–763.
   PubMed Web of Science ®Google Scholar
• Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, New York, N.Y.
   Google Scholar
• Horton, R. M., and L. R. Pease. 1991. Recombination and mutagenesis of DNA sequences using PCR. Directed mutagenesis—a practical approach.. M. J. McPherson. 217–249. Oxford University Press, New York, N.Y
   Google Scholar
• Kamibayashi, C., R. Estes, R. L. Licksteig, S. I. Yang, C. Craft, and M. C. Mumby. 1994. Comparison of heterotrimeric protein phosphatase 2A containing different B subunits. J. Biol. Chem. 269:20139–20148.
   PubMedGoogle Scholar
• Kearsey, S. E., and K. Labib. 1998. MCM proteins: evolution, properties, and role in DNA replication. Biochim. Biophys. Acta 1398:113–136.
   PubMed Web of Science ®Google Scholar
• Lin, X.-H., J. Walter, K. Scheidtmann, K. Ohst, J. Newport, and G. Walter. 1998. Protein phosphatase 2A is required for the initiation of chromosomal DNA replication. Proc. Natl. Acad. Sci. USA 95:14693–14698.
   PubMedGoogle Scholar
• Maga, G., M. Stucki, S. Spadari, and U. Hübscher. 2000. DNA polymerase switching. I. Replication factor C displaces DNA polymerase α prior to PCNA loading. J. Mol. Biol. 295:791–801.
   PubMedGoogle Scholar
• Mimura, S., and H. Takisawa. 1998. Xenopus Cdc45-dependent loading of DNA polymerase α onto chromatin under the control of S-phase cdk. EMBO J. 17:5699–5707.
   PubMed Web of Science ®Google Scholar
• Miyazawa, H., M. Izumi, S. Tada, R. Tadada, M. Masutani, M. Ui, and F. Hanaoka. 1993. Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase α-primase complex and their gene expression during cell proliferation and the cell cycle. J. Biol. Chem. 268:8111–8122.
   PubMed Web of Science ®Google Scholar
• Moarefi, I. F., D. Small, I. Gilbert, M. Höpfner, S. K. Randall, C. Schneider, A. A. R. Russo, U. Ramsperger, A. K. Arthur, H. Stahl, T. J. Kelly, and E. Fanning. 1993. Mutation of the cyclin-dependent kinase phosphorylation site in simian virus (SV40) large T antigen specifically blocks SV40 origin DNA unwinding. J. Virol. 67:4992–5002.
   PubMedGoogle Scholar
• Moses, K., and C. Prives. 1994. A unique subpopulation of murine DNA polymerase α/primase specifically interacts with polyomavirus T antigen and stimulates DNA replication. Mol. Cell. Biol. 14:2767–2776.
   PubMedGoogle Scholar
• Nasheuer, H.-P., and F. Grosse. 1987. Immunoaffinity-purified DNA polymerase α displays novel properties. Biochemistry 26:8458–8466.
   PubMedGoogle Scholar
• Nasheuer, H.-P., and F. Grosse. 1988. DNA polymerase α-primase from calf thymus. Determination of the polypeptide responsible for primase activity. J. Biol. Chem. 263:8981–8988.
   PubMedGoogle Scholar
• Nasheuer, H.-P., A. Moore, A. F. Wahl, and T. S.-F. Wang. 1991. Cell cycle-dependent phosphorylation of human DNA polymerase α. J. Biol. Chem. 266:7893–7903.
   PubMedGoogle Scholar
• Park, H., R. Davis, and T. S.-F. Wang. 1995. Studies of Schizosaccharomyces pombe DNA polymerase α at different stages of the cell cycle. Nucleic Acids Res. 23:4337–4344.
   PubMedGoogle Scholar
• Ruediger, R., J. E. van Wart Hood, M. Mumby, and G. Walter. 1991. Constant expression and activity of protein phosphatase 2A in synchronized cells. Mol. Cell. Biol. 11:4282–4285.
   PubMedGoogle Scholar
• Tanaka, S., S.-Z. Hu, T. S.-F. Wang, and D. Korn. 1982. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase α. J. Biol. Chem. 257:8386–8390.
   PubMedGoogle Scholar
• Tanaka, T., D. Knapp, and K. Nasmyth. 1997. Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell 90:649–660.
   PubMed Web of Science ®Google Scholar
• Tercero, J. A., K. Labib, and J. F. Diffley. 2000. DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p. EMBO J. 19:2082–2093.
   PubMedGoogle Scholar
• Todorov, I. T., A. Attaran, and S. E. Kearsey. 1995. BM28, a human member of the MCM2–3-5 family, is displaced from chromatin during DNA replication. J. Cell Biol. 129:1433–1445.
   PubMed Web of Science ®Google Scholar
• Virshup, D. M., A. Cegielska, A. Russo, T. J. Kelly, and S. Shaffer. 1993. The initiation of SV40 DNA replication is controlled by a phosphorylation-dephosphorylation cycle. Adv. Protein Phosphatases 7:271–293.
   Google Scholar
• Voitenleitner, C., E. Fanning, and H.-P. Nasheuer. 1997. Phosphorylation of DNA polymerase α-primase by cyclin A-dependent kinases regulates initiation of DNA replication in vitro. Oncogene 14:1611–1615.
   PubMedGoogle Scholar
• Voitenleitner, C., C. Rehfuess, M. Hilmes, L. O'Rear, P.-C. Liao, D. A. Gage, R. Ott, H.-P. Nasheuer, and E. Fanning. 1999. Cell cycle-dependent regulation of human DNA polymerase α-primase activity by phosphorylation. Mol. Cell. Biol. 19:646–656.
   PubMedGoogle Scholar
• Waga, S., and B. Stillman. 1994. Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro. Nature 369:207–212.
   PubMed Web of Science ®Google Scholar
• Wahl, A. F., A. M. Geis, B. H. Spain, S. W. Wong, D. Korn, and T. S.-F. Wang. 1988. Gene expression of human DNA polymerase α during cell proliferation and the cell cycle. Mol. Cell. Biol. 8:5016–5025.
   PubMedGoogle Scholar
• Walter, J., and J. Newport. 2000. Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of cdc45, RPA, and DNA polymerase α. Mol. Cell 5:617–627.
   PubMed Web of Science ®Google Scholar
• Wang, T. S.-F.. 1996. Cellular DNA polymerases. DNA replication in eukaryotic cells.. M. L. DePamphilis. 461–493. Cold Spring Harbor Laboratory Press, Plainview, N.Y
   Google Scholar
• Wera, S., and B. A. Hemmings. 1995. Serine/threonine protein phosphatases. Biochem. J. 311:17–29.
   PubMed Web of Science ®Google Scholar
• Wong, S. W., A. F. Wahl, P.-M. Yuan, N. Arai, B. E. Pearson, K. Arai, D. Korn, M. W. Hunkapiller, and T. S.-F. Wang. 1988. Human DNA polymerase α gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases. EMBO J. 7:37–47.
   PubMed Web of Science ®Google Scholar
• Zhu, T., S.-I. Matsuzawa, Y. Mizuno, C. Kamibayashi, M. C. Mumby, N. Andjelkovic, B. A. Hemmings, K. Onoe, and K. Kikuchi. 1997. The interconversion of protein phosphatase 2A between PP2A1 and PP2A0 during retinoic acid-induced granulocytic differentiation and a modification on the catalytic subunit in S phase of HL-60 cells. Arch. Biochem. Biophys. 339:210–217.
   PubMedGoogle Scholar