Identification of p21-Activated Kinase Specificity Determinants in Budding Yeast: a Single Amino Acid Substitution Imparts Ste20 Specificity to Cla4

REFERENCES

• Bagrodia, S., S. J. Taylor, C. L. Creasy, J. Chernoff, and R. A. Cerione. 1995. Identification of a mouse p21Cdc42/Rac activated kinase. J. Biol. Chem. 270: 22731–22737.
   PubMedGoogle Scholar
• Benton, B. K., A. Tinkelenberg, I. Gonzalez, and F. R. Cross. 1997. Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosis. Mol. Cell. Biol. 17: 5067–5076.
   PubMedGoogle Scholar
• Birukov, K. G., C. Csortos, L. Marzilli, S. Dudek, S. F. Ma, A. R. Bresnick, A. D. Verin, R. J. Cotter, and J. G. Garcia. 2001. Differential regulation of alternatively spliced endothelial cell myosin light chain kinase isoforms by p60(Src). J. Biol. Chem. 276: 8567–8573.
   PubMedGoogle Scholar
• Bose, I., J. E. Irazoqui, J. J. Moskow, E. S. Bardes, T. R. Zyla, and D. J. Lew. 2001. Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phosphorylation of Cdc24p. J. Biol. Chem. 276: 7176–7186.
   PubMed Web of Science ®Google Scholar
• Brown, J. L., L. Stowers, M. Baer, J. Trejo, S. Coughlin, and J. Chant. 1996. Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway. Curr. Biol. 6: 598–605.
   PubMed Web of Science ®Google Scholar
• Chant, J., and J. R. Pringle. 1995. Patterns of bud-site selection in the yeast Saccharomyces cerevisiae. J. Cell Biol. 129: 751–765.
   PubMedGoogle Scholar
• Cook, J. G., L. Bardwell, and J. Thorner. 1997. Inhibitory and activating functions for MAPK Kss1 in the S. cerevisiae filamentous-growth signalling pathway. Nature 390: 85–88.
   PubMedGoogle Scholar
• Cullen, P. J., and G. F. Sprague, Jr. 2000. Glucose depletion causes haploid invasive growth in yeast. Proc. Natl. Acad. Sci. USA 97: 13619–13624.
   PubMedGoogle Scholar
• Cvrckova, F., C. De Virgilio, E. Manser, J. R. Pringle, and K. Nasmyth. 1995. Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast. Genes Dev. 9: 1817–1830.
   PubMedGoogle Scholar
• Gulli, M., M. Jaquenoud, Y. Shimada, G. Niederhauser, P. Wiget, and M. Peter. 2000. Phosphorylation of the cdc42 exchange factor cdc24 by the PAK-like kinase cla4 may regulate polarized growth in yeast. Mol. Cell 6: 1155–1167.
   PubMed Web of Science ®Google Scholar
• Holly, S. P., and K. J. Blumer. 1999. PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae. J. Cell Biol. 147: 845–856.
   PubMed Web of Science ®Google Scholar
• Jarvis, E. E., D. C. Hagen, and G. F. Sprague, Jr. 1988. Identification of a DNA segment that is necessary and sufficient for α-specific gene control in Saccharomyces cerevisiae: implications for regulation of α-specific and a-specific genes. Mol. Cell. Biol. 8: 309–320.
   PubMedGoogle Scholar
• Kallunki, T., B. Su, I. Tsigelny, H. K. Sluss, B. Derijard, G. Moore, R. Davis, and M. Karin. 1994. JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation. Genes Dev. 8: 2996–3007.
   PubMed Web of Science ®Google Scholar
• Klinghoffer, R. A., C. Sachsenmaier, J. A. Cooper, and P. Soriano. 1999. Src family kinases are required for integrin but not PDGFR signal transduction. EMBO J. 18: 2459–2471.
   PubMed Web of Science ®Google Scholar
• Leberer, E., W. Cunle, T. Leeuw, A. Fourest-Lieuvin, J. Segall, and D. Thomas. 1997. Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO J. 16: 83–97.
   PubMedGoogle Scholar
• Leberer, E., D. Dignard, D. Harcus, L. Hougan, M. Whiteway, and D. Y. Thomas. 1993. Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1. Mol. Gen. Genet. 241: 241–254.
   PubMedGoogle Scholar
• Leberer, E., D. Dignard, D. Harcus, D. Y. Thomas, and M. Whiteway. 1992. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. EMBO J. 11: 4815–4824.
   PubMedGoogle Scholar
• Leeuw, T., C. Wu, J. D. Schrag, M. Whiteway, D. Y. Thomas, and E. Leberer. 1998. Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases. Nature 391: 191–195.
   PubMed Web of Science ®Google Scholar
• Lei, M., W. Lu, W. Meng, M. C. Parrini, M. J. Eck, B. J. Mayer, and S. C. Harrison. 2000. Structure of PAK1 in an autoinhibited conformation reveals a multistage activation switch. Cell 102: 387–397.
   PubMed Web of Science ®Google Scholar
• Longtine, M. S., A. McKenzie, D. J. Demarini, N. G. Shah, A. Wach, A. Brachat, P. Philippsen, and J. R. Pringle. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953–961.
   PubMed Web of Science ®Google Scholar
• Longtine, M. S., C. L. Theesfeld, J. N. McMillan, E. Weaver, J. R. Pringle, and D. J. Lew. 2000. Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 4049–4061.
   PubMed Web of Science ®Google Scholar
• Madhani, H. D., C. A. Styles, and G. R. Fink. 1997. MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell 91: 673–684.
   PubMed Web of Science ®Google Scholar
• Manser, E., T. Leung, H. Salihuddin, Z. S. Zhao, and L. Lim. 1994. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 367: 40–46.
   PubMed Web of Science ®Google Scholar
• Martin, H., A. Mendoza, J. M. Rodriguez-Pachon, M. Molina, and C. Nombela. 1997. Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK-like protein kinase. Mol. Microbiol. 23: 431–444.
   PubMedGoogle Scholar
• Mitchell, D. A., and G. F. Sprague, Jr. 2001. The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G2/M transition in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 488–500.
   PubMedGoogle Scholar
• Mosch, H. U., R. L. Roberts, and G. R. Fink. 1996. Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 93: 5352–5356.
   PubMed Web of Science ®Google Scholar
• Moskow, J. J., A. S. Gladfelter, R. E. Lamson, P. M. Pryciak, and D. J. Lew. 2000. Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 7559–7571.
   PubMed Web of Science ®Google Scholar
• O'Rourke, S. M., and I. Herskowitz. 1998. The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev. 12: 2874–2886.
   PubMed Web of Science ®Google Scholar
• Posas, F., E. A. Witten, and H. Saito. 1998. Requirement of STE50 for osmostress-induced activation of the STE11 mitogen-activated protein kinase kinase kinase in the high-osmolarity glycerol response pathway. Mol. Cell. Biol. 18: 5788–5796.
   PubMed Web of Science ®Google Scholar
• Printen, J. A., and G. F. Sprague, Jr. 1994. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics 138: 609–619.
   PubMedGoogle Scholar
• Raitt, D. C., F. Posas, and H. Saito. 2000. Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway. EMBO J. 19: 4623–4631.
   PubMedGoogle Scholar
• Ramer, S. W., and R. W. Davis. 1993. A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90: 452–456.
   PubMedGoogle Scholar
• Richman, T. J., M. M. Sawyer, and D. I. Johnson. 1999. The Cdc42p GTPase is involved in a G2/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast. J. Biol. Chem. 274: 16861–16870.
   PubMedGoogle Scholar
• Roberts, R. L., and G. R. Fink. 1994. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev. 8: 2974–2985.
   PubMedGoogle Scholar
• Rose, M. D., F. Winston, and P. Hieter (ed.). 1990. Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Santos, R. C., N. C. Waters, C. L. Creasy, and L. W. Bergman. 1995. Structure-function relationships of the yeast cyclin-dependent kinase Pho85. Mol. Cell. Biol. 15: 5482–5491.
   PubMedGoogle Scholar
• Sherman, F., G. R. Fink, and J. B. Hicks (ed.). 1982. Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Sheu, Y. J., Y. Barral, and M. Snyder. 2000. Polarized growth controls cell shape and bipolar bud site selection in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 5235–5247.
   PubMedGoogle Scholar
• Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19–27.
   PubMed Web of Science ®Google Scholar
• Smith, D. B., and K. S. Johnson. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31–40.
   PubMed Web of Science ®Google Scholar
• Sprague, G. F. 1991. Assay of yeast mating reaction. Methods Enzymol. 194: 77–93.
   PubMedGoogle Scholar
• Sreenivasan, A., and D. Kellogg. 1999. The elm1 kinase functions in a mitotic signaling network in budding yeast. Mol. Cell. Biol. 19: 7983–7994.
   PubMedGoogle Scholar
• Taniguchi, T. 1995. Cytokine signaling through nonreceptor protein tyrosine kinases. Science 268: 251–255.
   PubMed Web of Science ®Google Scholar
• Tjandra, H., J. Compton, and D. Kellogg. 1998. Control of mitotic events by the Cdc42 GTPase, the Clb2 cyclin and a member of the PAK kinase family. Curr. Biol. 8: 991–1000.
   PubMedGoogle Scholar
• Tu, H., and M. Wigler. 1999. Genetic evidence for Pak1 autoinhibition and its release by Cdc42. Mol. Cell. Biol. 19: 602–611.
   PubMed Web of Science ®Google Scholar
• Ushiro, H., T. Tsutsumi, K. Suzuki, T. Kayahara, and K. Nakano. 1998. Molecular cloning and characterization of a novel Ste20-related protein kinase enriched in neurons and transporting epithelia. Arch. Biochem. Biophys. 355: 233–240.
   PubMed Web of Science ®Google Scholar
• Wu, C., M. Whiteway, D. Thomas, and E. Lebere. 1995. Molecular characterization of Ste20, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. J. Biol. Chem. 270: 15984–15992.
   PubMedGoogle Scholar
• Zhou, Z., A. Gartner, R. Cade, G. Ammerer, and B. Errede. 1993. Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases. Mol. Cell. Biol. 13: 2069–2080.
   PubMedGoogle Scholar