Recycling of the U12-Type Spliceosome Requires p110, a Component of the U6atac snRNP

REFERENCES

• Achsel, T., Brahms H., Kastner B., Bachi A., Wilm M., and Lührmann R.. 1999. A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. EMBO J. 18:5789–5802.
   PubMed Web of Science ®Google Scholar
• Bell, M., and Bindereif A.. 1999. Cloning and mutational analysis of the Leptomonas seymouri U5 snRNA gene: function of the Sm site in core RNP formation and nuclear localization. Nucleic Acids Res. 27:3986–3994.
   PubMed Web of Science ®Google Scholar
• Bell, M., Schreiner S., Damianov A., Reddy R., and Bindereif A.. 2002. p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor. EMBO J. 21:2724–2735.
   PubMed Web of Science ®Google Scholar
• Bindereif, A., Wolff T., and Green M. R.. 1990. Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes. EMBO J. 9:251–255.
   PubMedGoogle Scholar
• Brow, D. A. 2002. Allosteric cascade of spliceosome activation. Annu. Rev. Genet. 36:333–360.
   PubMed Web of Science ®Google Scholar
• Burge, C. B., Padgett R. A., and Sharp P. A.. 1998. Evolutionary fates and origins of U12-type introns. Mol. Cell 2:773–785.
   PubMedGoogle Scholar
• Burge, C. B., Tuschl T., and Sharp P. A.. 1999. Splicing of precursors to mRNAs by the spliceosomes, p. 525–560. In Gesteland R. F., Cech T. R., and Atkins J. F. (ed.), The RNA world. Cold Spring Harbor Press, New York, N.Y.
   Google Scholar
• Ghetti, A., Company M., and Abelson J.. 1995. Specificity of Prp24 binding to RNA: a role for Prp24 in the dynamic interaction of U4 and U6 snRNAs. RNA 1:132–145.
   PubMedGoogle Scholar
• Hall, S. L., and Padgett R. A.. 1996. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. Science 271:1716–1718.
   PubMed Web of Science ®Google Scholar
• Hartmuth, K., Urlaub H., Vornlocher H. P., Will C. L., Gentzel M., Wilm M., and Lührmann R.. 2002. Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method. Proc. Natl. Acad. Sci. USA 99:16719–16724.
   PubMed Web of Science ®Google Scholar
• Incorvaia, R., and Padgett R. A.. 1998. Base pairing with U6atac snRNA is required for 5′ splice site activation of U12-dependent introns in vivo. RNA 4:709–718.
   PubMedGoogle Scholar
• Jurica, M. S., Licklider L. J., Gygi S. R., Grigorieff N., and Moore M. J.. 2002. Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA 8:426–439.
   PubMed Web of Science ®Google Scholar
• Kambach, C., Walke S., and Nagai K.. 1999. Structure and assembly of the spliceosomal small nuclear ribonucleoprotein particles. Curr. Opin. Struct. Biol. 9:222–230.
   PubMed Web of Science ®Google Scholar
• Kolossova, I., and Padgett R. A.. 1997. U11 snRNA interacts in vivo with the 5′ splice site of U12-dependent (AU-AC) pre-mRNA introns. RNA 3:227–233.
   PubMedGoogle Scholar
• Krainer, A. R., Maniatis T., Ruskin B., and Green M. R.. 1984. Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell 36:993–1005.
   PubMedGoogle Scholar
• Lelay-Taha, M. N., Reveillaud I., Sri-Widada J., Brunel C., and Jeanteur P.. 1986. RNA-protein organization of U1, U5 and U4-U6 small nuclear ribonucleoproteins in HeLa cells. J. Mol. Biol. 189:519–532.
   PubMed Web of Science ®Google Scholar
• Levine, A., and Durbin R.. 2001. A computational scan for U12-dependent introns in the human genome sequence. Nucleic Acids Res. 29:4006–4013.
   PubMedGoogle Scholar
• Lücke, S., Klöckner T., Palfi Z., Boshart M., and Bindereif A.. 1997. Trans mRNA splicing in trypanosomes: cloning and analysis of a PRP8-homologous gene from Trypanosoma brucei provides evidence for a U5-analogous RNP. EMBO J. 16:4433–4440.
   PubMedGoogle Scholar
• Mougin, A., Gottschalk A., Fabrizio P., Lührmann R., and Branlant C.. 2002. Direct probing of RNA structure and RNA-protein interactions in purified HeLa cells and yeast spliceosomal U4/U6.U5 tri-snRNP particles. J. Mol. Biol. 317:631–649.
   PubMedGoogle Scholar
• Nottrott, S., Hartmuth K., Fabrizio P., Urlaub H., Vidovic I., Ficner R., and Lührmann R.. 1999. Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5′ stem-loop of U4 snRNA. EMBO J. 18:6119–6133.
   PubMedGoogle Scholar
• Nottrott, S., Urlaub H., and Lührmann R.. 2002. Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins. EMBO J. 21:5527–5538.
   PubMed Web of Science ®Google Scholar
• Padgett, R. A., and Shukla G. C.. 2002. A revised model for U4atac/U6atac snRNA base pairing. RNA 8:125–128.
   PubMedGoogle Scholar
• Patel, A. A., McCarthy M., and Steitz J. A.. 2002. The splicing of U12-type introns can be a rate-limiting step in gene expression. EMBO J. 21:3804–3815.
   PubMed Web of Science ®Google Scholar
• Rader, S. D., and Guthrie C.. 2002. A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation. RNA 8:1378–1392.
   PubMedGoogle Scholar
• Raghunathan, P. L., and Guthrie C.. 1998. A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles. Science 279:857–860.
   PubMed Web of Science ®Google Scholar
• Schneider, C., Will C. L., Makarova O. V., Makarov E. M., and Lührmann R.. 2002. Human U4/U6.U5 and U4atac/U6atac.U5 tri-snRNPs exhibit similar protein compositions. Mol. Cell. Biol. 22:3219–3229.
   PubMedGoogle Scholar
• Shannon, K. W., and Guthrie C.. 1991. Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA binding motifs. Genes Dev. 5:773–785.
   PubMedGoogle Scholar
• Sharp, P. A., and Burge C. B.. 1997. Classification of introns: U2-type or U12-type. Cell 91:875–879.
   PubMedGoogle Scholar
• Shukla, G. C., Cole A. J., Dietrich R. C., and Padgett R. A.. 2002. Domains of human U4atac snRNA required for U12-dependent splicing in vivo. Nucleic Acids Res. 30:4650–4657.
   PubMedGoogle Scholar
• Stanek, D., Rader S. D., Klingauf M., and Neugebauer K. M.. 2002. Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies. J. Cell Biol. 160:505–516.
   Google Scholar
• Tarn, W. Y., and Steitz J. A.. 1996. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns. Science 273:1824–1832.
   PubMedGoogle Scholar
• Tarn, W. Y., and Steitz J. A.. 1996. A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro. Cell 84:801–811.
   PubMedGoogle Scholar
• Tarn, W. Y., and Steitz J. A.. 1997. Pre-mRNA splicing: the discovery of a new spliceosome doubles the challenge. Trends Biochem. Sci. 22:132–137.
   PubMed Web of Science ®Google Scholar
• Vidaver, R. M., Fortner, Loos-Austin M. D., Loos-Austin S. L., and Brow D. A.. 1999. Multiple functions of Saccharomyces cerevisiae splicing protein Prp24 in U6 RNA structural rearrangements. Genetics 153:1205–1218.
   PubMedGoogle Scholar
• Wersig, C., and Bindereif A.. 1990. Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly. Nucleic Acids Res. 18:6223–6229.
   PubMed Web of Science ®Google Scholar
• Will, C. L., and Lührmann R.. 2001. Spliceosomal UsnRNP biogenesis, structure and function. Curr. Opin. Cell Biol. 13:290–301.
   PubMed Web of Science ®Google Scholar
• Will, C. L., Schneider C., Reed R., and Lührmann R.. 1999. Identification of both shared and distinct proteins in the major and minor spliceosomes. Science 284:2003–2005.
   PubMed Web of Science ®Google Scholar
• Wu, Q., and Krainer A. R.. 1999. AT-AC pre-mRNA splicing mechanisms and conservation of minor introns in voltage-gated ion channel genes. Mol. Cell. Biol. 19:3225–3236.
   PubMed Web of Science ®Google Scholar
• Wu, Q., and Krainer A. R.. 1998. Purine-rich enhancers function in the AT-AC pre-mRNA splicing pathway and do so independently of intact U1 snRNP. RNA 4:1664–1673.
   PubMedGoogle Scholar
• Yu, Y.-T., Scharl E. C., Smith C. M., and Steitz J. A.. 1999. The growing world of small nuclear ribonucleoproteins, p. 487–524. In Gesteland R. F., Cech T. R., and Atkins J. F. (ed.), The RNA world. Cold Spring Harbor Press, New York, N.Y.
   Google Scholar
• Zhou, Z., Licklider L. J., Gygi S. P., and Reed R.. 2002. Comprehensive proteomic analysis of the human spliceosome. Nature 419:182–185.
   PubMed Web of Science ®Google Scholar