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Transcriptional Regulation

Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro

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Pages 3324-3336 | Received 10 Sep 2003, Accepted 11 Dec 2003, Published online: 27 Mar 2023

REFERENCES

  • Andrulis, E. D., Guzman E., Doring P., Werner J., and Lis J. T.. 2000. High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. Genes Dev. 14:2635–2649.
  • Andrulis, E. D., Werner J., Nazarian A., Erdjument-Bromage H., Tempst P., and Lis J. T.. 2002. The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila. Nature 420:837–841.
  • Bortvin, A., and Winston F.. 1996. Evidence that Spt6p controls chromatin structure by a direct interaction with histones. Science 272:1473–1476.
  • Bourgeois, C. F., Kim Y. K., Churcher M. J., West M. J., and Karn J.. 2002. Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences. Mol. Cell. Biol. 22:1079–1093.
  • Compagnone-Post, P. A., and Osley M. A.. 1996. Mutations in the SPT4, SPT5, and SPT6 genes alter transcription of a subset of histone genes in Saccharomyces cerevisiae. Genetics 143:1543–1554.
  • Dignam, J. D., Lebovitz R. M., and Roeder R. G.. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475–1489.
  • Harlow E. D., and Lane D.. 1998. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, New York, N.Y.
  • Hartzog, G. A., Wada T., Handa H., and Winston F.. 1998. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev. 12:357–369.
  • Hasegawa, M., Endou T., Narita T., Yamada Y., Yamaguchi T., Wada T., and Handa H.. 2003. A rapid purification method for human RNA polymerase II by two-step affinity chromatography. J. Biochem. 133:133–138.
  • Ivanov, D., Kwak Y. T., Guo J., and Gaynor R. B.. 2000. Domains in the SPT5 protein that modulate its transcriptional regulatory properties. Mol. Cell. Biol. 20:2970–2983.
  • Kaplan, C. D., Morris J. R., Wu C., and Winston F.. 2000. Spt5 and Spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. Genes Dev. 14:2623–2634.
  • Kaplan, C. D., Laprade L., and Winston F.. 2003. Transcription elongation factors repress transcription initiation from cryptic sites. Science 301:1096–1099.
  • Keegan, B. R., Feldman J. L., Lee D. H., Koos D. S., Ho R. K., Stainier D. Y., and Yelon D.. 2002. The elongation factors Pandora/Spt6 and Foggy/Spt5 promote transcription in the zebra fish embryo. Development 129:1623–1632.
  • Kim, D. K., Inukai N., Yamada T., Furuya A., Sato H., Yamaguchi Y., Wada T., and Handa H.. 2003. Requirement for human Spt4 in RNA polymerase II elongation control in vitro. Genes Cells 8:371–378.
  • Krogan, N. J., Kim M., Ahn S. H., Zhong G., Kobor M. S., Cagney G., Emili A., Shilatifard A., Buratowski S., and Greenblatt J. F.. 2002. RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol. Cell. Biol. 22:6979–6992.
  • Lindstrom, D. L., Squazzo S. L., Muster N., Burckin T. A., Wachter K. C., Emigh C. A., McCleery J. A., Yates III J. R., and Hartzog G. A.. 2003. Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol. Cell. Biol. 23:1368–1378.
  • Mandal, S. S., Cho H., Kim S., Cabane K., and Reinberg D.. 2002. FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation. Mol. Cell. Biol. 22:7543–7552.
  • Parada, C. A., and Roeder R. G.. 1999. A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription. EMBO J. 18:3688–3701.
  • Renner, D. B., Yamaguchi Y., Wada T., Handa H., and Price D. H.. 2001. A highly purified RNA polymerase II elongation control system. J. Biol. Chem. 276:42601–42609.
  • Samkurashvili, I., and Luse D. S.. 1998. Structural changes in the RNA polymerase II transcription complex during transition from initiation to elongation. Mol. Cell. Biol. 18:5343–5354.
  • Saunders, A., Werner J., Andrulis E. D., Nakayama T., Hirose S., Reinberg D., and Lis J.. 2003. Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo. Science 301:1094–1096.
  • Sawada, J., Goto M., Sawa C., Watanabe H., and Handa H.. 1994. Transcriptional activation through the tetrameric complex formation of E4TF1 subunits. EMBO J. 13:1396–1402.
  • Svejstrup, J. Q. 2003. Transcription. Histones face the FACT. Science 301:1053–1055.
  • Swanson, M. S., Carlson M., and Winston F.. 1990. SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus. Mol. Cell. Biol. 10:4935–4941.
  • Wada, T., Takagi T., Yamaguchi Y., Ferdous A., Imai T., Hirose S., Sugimoto S., Yano K., Hartzog G. A., Winston F., Buratowski S., and Handa H.. 1998. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs. Genes Dev. 12:343–356.
  • Wada, T., Takagi T., Yamaguchi Y., Watanabe D., and Handa H.. 1998. Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro. EMBO J. 17:7395–7403.
  • Winkler, M., aus Dem Siepen T., and Stamminger T.. 2000. Functional interaction between pleiotropic transactivator pUL69 of human cytomegalovirus and the human homolog of yeast chromatin regulatory protein SPT6. J. Virol. 74:8053–8064.
  • Winston, F. 1992. Analysis of SPT genes: a genetic approach toward analysis of TFIID, histones, and other transcription factors of yeast, p. 1271–1293. In McKnight S. L. and Yamamoto K. R. (ed.), Transcriptional regulation. Cold Spring Harbor Laboratory Press, New York, N.Y.
  • Wu, C. H., Yamaguchi Y., Benjamin L. R., Horvat-Gordon M., Washinsky J., Enerly E., Larsson J., Lambertsson A., Handa H., and Gilmour D.. 2003. NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 17:1402–1414.
  • Wu-Baer, F., Lane W. S., and Gaynor R. B.. 1998. Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation. J. Mol. Biol. 27:179–197.
  • Yamaguchi, Y., Wada T., Watanabe D., Takagi T., Hasegawa J., and Handa H.. 1999. Structure and function of the human transcription elongation factor DSIF. J. Biol. Chem. 274:8085–8092.
  • Yamaguchi, Y., Takagi T., Wada T., Yano K., Furuya A., Sugimoto S., Hasegawa J., and Handa H.. 1999. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation. Cell 97:41–51.
  • Yamaguchi, Y., Inukai N., Narita T., Wada T., and Handa H.. 2002. Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA. Mol. Cell. Biol. 22:2918–2927.

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