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Chromosome Structure and Dynamics

Mutations of the Yku80 C Terminus and Xrs2 FHA Domain Specifically Block Yeast Nonhomologous End Joining

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Pages 10782-10790 | Received 23 Jul 2005, Accepted 23 Sep 2005, Published online: 27 Mar 2023

REFERENCES

  • Bertuch, A. A., and V. Lundblad. 2003. The Ku heterodimer performs separable activities at double-strand breaks and chromosome termini. Mol. Cell. Biol. 23:8202–8215.
  • Boulton, S. J., and S. P. Jackson. 1998. Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J. 17:1819–1828.
  • Boulton, S. J., and S. P. Jackson. 1996. Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance. Nucleic Acids Res. 24:4639–4648.
  • Brachmann, C. B., A. Davies, G. J. Cost, E. Caputo, J. Li, P. Hieter, and J. D. Boeke. 1998. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14:115–132.
  • Chen, L., K. Trujillo, W. Ramos, P. Sung, and A. E. Tomkinson. 2001. Promotion of Dnl4-catalyzed DNA end-joining by the Rad50/Mre11/Xrs2 and Hdf1/Hdf2 complexes. Mol. Cell 8:1105–1115.
  • Daley, J. M., and T. E. Wilson. 2005. Rejoining of DNA double-strand breaks as a function of overhang length. Mol. Cell. Biol. 25:896–906.
  • D'Amours, D., and S. P. Jackson. 2002. The mre11 complex: at the crossroads of DNA repair and checkpoint signalling. Nat. Rev. Mol. Cell Biol. 3:317–327.
  • DeFazio, L. G., R. M. Stansel, J. D. Griffith, and G. Chu. 2002. Synapsis of DNA ends by DNA-dependent protein kinase. EMBO J. 21:3192–3200.
  • Della, M., P. L. Palmbos, H. M. Tseng, L. M. Tonkin, J. M. Daley, L. M. Topper, R. S. Pitcher, A. E. Tomkinson, T. E. Wilson, and A. J. Doherty. 2004. Mycobacterial Ku and ligase proteins constitute a two-component NHEJ repair machine. Science 306:683–685.
  • Dore, A. S., A. C. Drake, S. C. Brewerton, T. L. Blundell, and A. M. Robert. 2004. Identification of DNA-PK in the arthropods. Evidence for the ancient ancestry of vertebrate non-homologous end-joining. DNA Repair 3:33–41.
  • Dudasova, Z., A. Dudas, and M. Chovanec. 2004. Non-homologous end-joining factors of Saccharomyces cerevisiae. FEMS Microbiol. Rev. 28:581–601.
  • Durocher, D., I. A. Taylor, D. Sarbassova, L. F. Haire, S. L. Westcott, S. P. Jackson, S. J. Smerdon, and M. B. Yaffe. 2000. The molecular basis of FHA domain:phosphopeptide binding specificity and implications for phospho-dependent signaling mechanisms. Mol. Cell 6:1169–1182.
  • Falck, J., J. Coates, and S. P. Jackson. 2005. Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature 434:605–611.
  • Frank-Vaillant, M., and S. Marcand. 2001. NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway. Genes Dev. 15:3005–3012.
  • Furuta, T., H. Takemura, Z. Y. Liao, G. J. Aune, C. Redon, O. A. Sedelnikova, D. R. Pilch, E. P. Rogakou, A. Celeste, H. T. Chen, A. Nussenzweig, M. I. Aladjem, W. M. Bonner, and Y. Pommier. 2003. Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes. J. Biol. Chem. 278:20303–20312.
  • Gell, D., and S. P. Jackson. 1999. Mapping of protein-protein interactions within the DNA-dependent protein kinase complex. Nucleic Acids Res. 27:3494–3502.
  • Reference deleted.
  • Harris, R., D. Esposito, A. Sankar, J. D. Maman, J. A. Hinks, L. H. Pearl, and P. C. Driscoll. 2004. The 3D solution structure of the C-terminal region of Ku86 (Ku86CTR). J. Mol. Biol. 335:573–582.
  • Hopfner, K. P., C. D. Putnam, and J. A. Tainer. 2002. DNA double-strand break repair from head to tail. Curr. Opin. Struct. Biol. 12:115–122.
  • Jackson, S. P. 2002. Sensing and repairing DNA double-strand breaks. Carcinogenesis 23:687–696.
  • Karathanasis, E., and T. E. Wilson. 2002. Enhancement of Saccharomyces cerevisiae end-joining efficiency by cell growth stage but not by impairment of recombination. Genetics 161:1015–1027.
  • Kobayashi, J., H. Tauchi, S. Sakamoto, A. Nakamura, K. Morishima, S. Matsuura, T. Kobayashi, K. Tamai, K. Tanimoto, and K. Komatsu. 2002. NBS1 localizes to γ-H2AX foci through interaction with the FHA/BRCT domain. Curr. Biol. 12:1846–1851.
  • Koch, C. A., R. Agyei, S. Galicia, P. Metalnikov, P. O'Donnell, A. Starostine, M. Weinfeld, and D. Durocher. 2004. XRCC4 physically links DNA end processing by polynucleotide kinase to DNA ligation by DNA ligase IV. EMBO J. 23:3874–3885.
  • Kupiec, M. 2000. Damage-induced recombination in the yeast Saccharomyces cerevisiae. Curr. Genet. 38:23–32.
  • Lewis, L. K., F. Storici, S. Van Komen, S. Calero, P. Sung, and M. A. Resnick. 2004. Role of the nuclease activity of Saccharomyces cerevisiae Mre11 in repair of DNA double-strand breaks in mitotic cells. Genetics 166:1701–1713.
  • Liu, C., J. J. Pouliot, and H. A. Nash. 2002. Repair of topoisomerase I covalent complexes in the absence of the tyrosyl-DNA phosphodiesterase Tdp1. Proc. Natl. Acad. Sci. USA 99:14970–14975.
  • Ma, J. L., E. M. Kim, J. E. Haber, and S. E. Lee. 2003. Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences. Mol. Cell. Biol. 23:8820–8828.
  • Ma, Y., U. Pannicke, K. Schwarz, and M. R. Lieber. 2002. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell 108:781–794.
  • Meek, K., S. Gupta, D. A. Ramsden, and S. P. Lees-Miller. 2004. The DNA-dependent protein kinase: the director at the end. Immunol. Rev. 200:132–141.
  • Nugent, C. I., G. Bosco, L. O. Ross, S. K. Evans, A. P. Salinger, J. K. Moore, J. E. Haber, and V. Lundblad. 1998. Telomere maintenance is dependent on activities required for end repair of double-strand breaks. Curr. Biol. 8:657–660.
  • Polotnianka, R. M., J. Li, and A. J. Lustig. 1998. The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activities. Curr. Biol. 8:831–834.
  • Rost, B. 2004. The PredictProtein server. Nucleic Acids Res. 32:W517–W521.
  • Roy, R., B. Meier, A. D. McAinsh, H. M. Feldmann, and S. P. Jackson. 2004. Separation-of-function mutants of yeast Ku80 reveal a Yku80p-Sir4p interaction involved in telomeric silencing. J. Biol. Chem. 279:86–94.
  • Rupp, S. 2002. LacZ assays in yeast. Methods Enzymol. 350:112–131.
  • Shima, H., M. Suzuki, and M. Shinohara. 2005. Isolation and characterization of novel xrs2 mutations in Saccharomyces cerevisiae. Genetics 170:71–85.
  • Reference deleted.
  • Taddei, A., F. Hediger, F. R. Neumann, C. Bauer, and S. M. Gasser. 2004. Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins. EMBO J. 23:1301–1312.
  • Tauchi, H., J. Kobayashi, K. Morishima, S. Matsuura, A. Nakamura, T. Shiraishi, E. Ito, D. Masnada, D. Delia, and K. Komatsu. 2001. The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50-hMRE11-NBS1 complex DNA repair activity. J. Biol. Chem. 276:12–15.
  • Teo, S. H., and S. P. Jackson. 2000. Lif1p targets the DNA ligase Lig4p to sites of DNA double-strand breaks. Curr. Biol. 10:165–168.
  • Tseng, H. M., and A. E. Tomkinson. 2002. A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining. J. Biol. Chem. 277:45630–45637.
  • Tsukamoto, Y., C. Mitsuoka, M. Terasawa, H. Ogawa, and T. Ogawa. 2005. Xrs2p regulates mre11p translocation to the nucleus and plays a role in telomere elongation and meiotic recombination. Mol. Biol. Cell 16:597–608.
  • Walker, J. R., R. A. Corpina, and J. Goldberg. 2001. Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair. Nature 412:607–614.
  • Wilson, T. E. 2002. A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162:677–688.
  • Wilson, T. E., U. Grawunder, and M. R. Lieber. 1997. Yeast DNA ligase IV mediates non-homologous DNA end joining. Nature 388:495–498.
  • Wilson, T. E., and M. R. Lieber. 1999. Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase β (Pol4)-dependent pathway. J. Biol. Chem. 274:23599–23609.
  • Winzeler, E. A., D. D. Shoemaker, A. Astromoff, H. Liang, K. Anderson, B. Andre, R. Bangham, R. Benito, J. D. Boeke, H. Bussey, A. M. Chu, C. Connelly, K. Davis, F. Dietrich, S. W. Dow, M. El Bakkoury, F. Foury, S. H. Friend, E. Gentalen, G. Giaever, J. H. Hegemann, T. Jones, M. Laub, H. Liao, R. W. Davis, et al. 1999. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285:901–906.
  • Zhang, Z., W. Hu, L. Cano, T. D. Lee, D. J. Chen, and Y. Chen. 2004. Solution structure of the C-terminal domain of Ku80 suggests important sites for protein-protein interactions. Structure (Cambridge) 12:495–502.

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