Rearrangement and Junctional-Site Sequence Analyses of T-Cell Receptor Gamma Genes in Intestinal Intraepithelial Lymphocytes from Murine Athymic Chimeras

REFERENCES

• Asarnow, D. M., T. Goodman, L. LeFrancois, and J. P. Allison. 1989. Distinct antigen receptor repertoires of two classes of murine epithelium-associated Τ cells. Nature (London) 341:60-62.
   PubMedGoogle Scholar
• Asarnow, D. M., W. A. Kuziel, M. Bonyhadi, R. E. Tigelaar, P. W. Tucker, and J. P. Allison. 1988. Limited diversity of γδ antigen receptor genes of Thy-1+ dendritic epidermal cells. Cell 55:837-847.
   PubMed Web of Science ®Google Scholar
• Bandeira, Α., Τ. Mota-Santos, S. Itohara, S. Degermann, C. Heusser, S. Tonegawa, and A. Coutinho. 1990. Localization of γ/δ Τ cells in the intestinal epithelium is independent of normal microbial colonization. J. Exp. Med. 172:239-244.
   PubMed Web of Science ®Google Scholar
• Bevan, M. J. 1977. In a radiation chimera host H-2 antigens determine the immune responsiveness of donor cytotoxic cells. Nature (London) 269:417-418.
   PubMed Web of Science ®Google Scholar
• Blackman, M., J. Kappler, and P. Marrack. 1990. The role of the Τ cell receptor in positive and negative selection of developing Τ cells. Science 248:1335-1341.
   PubMed Web of Science ®Google Scholar
• Bonneville, M., S. Itohara, E. G. Krecko, P. Mombaerts, I. Ishida, M. Katsuti, A. Berns, A. G. Farr, C. A. Janeway, and S. Tonegawa. 1990. Transgenic mice demonstrate that epithelial homing of γ/δ Τ cells is determined by cell lineages independent of Τ cell receptor specificity. J. Exp. Med. 171:1015-1026.
   PubMedGoogle Scholar
• Bruce, J., F. W. Symington, T. J. McKearn, and J. Sprent. 1981. A monoclonal antibody discriminating between subsets of Τ and Β cells. J. Immunol. 127:2496-2501.
   PubMed Web of Science ®Google Scholar
• Ceredig, R., J. W. Lowenthal, M. Nabholz, and H. R. MacDonald. 1985. Expression of interleukin 2 as a differentiation marker on intrathymic stem cells. Nature (London) 314:98-100.
   PubMed Web of Science ®Google Scholar
• Davis, M. 1990. Τ cell receptor gene diversity and selection. Annu. Rev. Biochem. 59:475-496.
   PubMedGoogle Scholar
• De Geus, B., M. Van den Enden, C. Coolen, L. Nagelkerken, P. Van der Jeijden, and J. Rozing. 1990. Phenotype of intraepithelial lymphocytes in euthymic mice: implications for differentiation of cells bearing a CD3-associated γδ Τ cell receptor. Eur. J. Immunol. 20:291-298.
   PubMedGoogle Scholar
• Fichtelius, Κ. Ε. 1967. The gut epithelium—a first level lymphoid organ? Exp. Cell. Res. 49:87-92.
   Google Scholar
• Fichtelius, Κ. Ε., Ε. J. Yunis, and R. A. Good. 1968. Occurrence of lymphocytes within the gut epithelium of normal and neonatally thymectomized mice. Proc. Soc. Exp. Biol. Med. 128:185-188.
   PubMed Web of Science ®Google Scholar
• Fry, A. M., L. A. Jones, A. M. Kruisbeek, and L. A. Matis. 1989. Thymic requirement for clonal deletion during Τ cell development. Science 246:1044-1046.
   PubMed Web of Science ®Google Scholar
• Garman, R. D., P. J. Doherty, and D. H. Raulet. 1986. Diversity, rearrangement, and expression of murine Τ cell gamma genes. Cell 45:733-742.
   PubMedGoogle Scholar
• Guy-Grand, D., N. Cerf-Bensussan, B. Malissen, M. Malassis-Seris, C. Briottet, and P. Vassalli. 1991. Two gut intraepithelial CD8+ lymphocyte populations with different Τ cell receptors: a role for the gut epithelium in Τ cell differentiation. J. Exp. Med. 173:471-481.
   PubMed Web of Science ®Google Scholar
• Havran, W. L., and J. P. Allison. 1988. Developmentally ordered appearance of thymocytes expressing different T-cell antigen receptors. Nature (London) 335:443-445.
   PubMed Web of Science ®Google Scholar
• Heilig, J. S., and S. Tonegawa. 1987. T-cell γ gene is allelically but not isotypically excluded and is not required in known functional T-cell subsets. Proc. Natl. Acad. Sci. USA 84:8070-8074.
   PubMedGoogle Scholar
• Hershberg, R., P. Eghtesady, B. Sydora, K. Brorson, H. Cheroutre, R. Modlin, and M. Kronenberg. 1990. Expression of the thymus leukemia antigen in mouse intestinal epithelium. Proc. Natl. Acad. Sci. USA 87:9727-9731.
   PubMedGoogle Scholar
• Hodes, R. J., S. O. Sharrow, and A. Solomon. 1989. Failure of Τ cell receptor Vβ negative selection in an athymic environment. Science 246:1041-1044.
   PubMedGoogle Scholar
• Innis, Μ. Α., Κ. Muambo, D. H. Gelfand, and M. A. Brow. 1988. DNA sequencing with Thermus aquaticus DNA polymerase and direct polymerase chain reaction-amplified DNA. Proc. Natl. Acad. Sci. USA 85:9436-9440.
   PubMed Web of Science ®Google Scholar
• Itohara, S., and S. Tonegawa. 1990. Selection of γδ Τ cells with canonical T-cell antigen receptors in fetal thymus. Proc. Natl. Acad. Sci. USA 87:7935-7938.
   PubMedGoogle Scholar
• Klein, J. R. 1986. Ontogeny of the Thy-1-, Lyt-2+ murine intestinal intraepithelial lymphocyte. Characterization of a unique population of thymus-independent cytotoxic effector cells in the intestinal mucosa. J. Exp. Med. 164:306-314.
   Google Scholar
• Klein, J. R., R. L. Mosley, and D. Kaiserlian. 1990. Expression of the asialo GM1 determinant on murine intestinal epithelia. Proc Soc. Exp. Biol. Med. 195:329-334.
   PubMedGoogle Scholar
• Kranz, D. M., H. Saito, M. Heller, Y. Takagaki, W. Haas, Η. Ν. Eisen, and S. Tonegawa. 1985. Limited diversity of the rearranged T-cell 7 gene. Nature (London) 313:752-754.
   PubMedGoogle Scholar
• Kyes, S., E. Carew, S. R. Carding, C. A. Janeway, and A. Hayday. 1989. Diversity in T-cell receptor γ gene usage in intestinal epithelium. Proc. Natl. Acad. Sci. USA 86:5227-5531.
   PubMedGoogle Scholar
• Lafaille, J. J., A. DeCloux, M. Bonneville, Y. Takagaki, and S. Tonegawa. 1989. Junctional sequences of the Τ cell receptor yb genes: implications for γδ Τ cell lineages and for a novel intermediate of V-(D)-J joining. Cell 59:859-870.
   PubMed Web of Science ®Google Scholar
• Lafaille, J. J., W. Haas, A. Coutinho, and S. Tonegawa. 1990. Positive selection of γδ Τ cells. Immunol. Today 11:75-78.
   PubMedGoogle Scholar
• MacDonald, H. R., R. K. Lees, R. Schneider, R. M. Zinkernagel, and H. Hengartner. 1988. Nature (London) 336:471-473.
   PubMed Web of Science ®Google Scholar
• Mayerhofer, G. 1980. Thymus-dependent and thymus-independent subpopulations of intestinal intraepithelial lymphocytes: a granulated subpopulation of probable bone marrow origin. Blood 55:532-535.
   PubMedGoogle Scholar
• Mosley, R. L., D. Styre, and J. R. Klein. 1990. Differentiation and functional maturation of bone marrow derived intestinal epithelial Τ cells expressing membrane Τ cell receptor in athymic radiation chimeras. J. Immunol. 145:1369-1375.
   PubMed Web of Science ®Google Scholar
• Mosley, R. L., D. Styre, and J. R. Klein. 1990. CD4+CD8+ murine intestinal intraepithelial lymphocytes. Int. Immunol. 2:361-365.
   PubMedGoogle Scholar
• Murray, V. 1989. Improved double-stranded DNA sequencing using linear polymerase chain reaction. Nucleic Acids. Res. 17:8889-8891.
   PubMed Web of Science ®Google Scholar
• Raulet, D. H. 1989. Structure, function, and molecular genetics of the γ/δ Τ cell receptor. Annu. Rev. Immunol. 7:175-207.
   PubMed Web of Science ®Google Scholar
• Reilly, E. B., D. M. Kranz, S. Tonegawa, and Η. Ν. Eisen. 1986. A functional γ gene formed from γ-gene segments is not necessary for antigen-specific responses of murine cytotoxic Τ lymphocytes. Nature (London) 321:878-880.
   PubMedGoogle Scholar
• Saiki, R. K., S. Scharf, F. Faloona, K. B. Mullis, G. T. Horn, H. A. Erlich, and N. Arnheim. 1985. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase? Science 239:487.
   Google Scholar
• Saito, H., D. M. Kranz, Y. Takagaki, A. C. Hayday, Η. Ν. Eisen, and S. Tonegawa. 1984. A third rearranged and expressed gene in a clone of cytotoxic Τ lymphocytes. Nature (London) 309:787-762.
   PubMedGoogle Scholar
• Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed., p. 916-917. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
   PubMed Web of Science ®Google Scholar
• Sarmiento, M., A. L. Glasebrook, and F. Fitch. 1980. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block Τ cell-mediated cytolysis in the absence of complement. J. Immunol. 125:2665-2672.
   PubMedGoogle Scholar
• Sim, G. K., and A. Augustin. 1991. Extrathymic positive selection of γδ Τ cells. Vγ4Jγ1 rearrangements with "GxYS" junctions. J. Immunol. 146:2439-2445.
   Google Scholar
• Sprent, J., and S. Webb. 1987. Function and specificity of Τ cell subsets in the mouse. Adv. Immunol. 41:39-133.
   PubMedGoogle Scholar
• Springer, T., G. Galfre, D. S. Secher, and C. Milstein. 1978. Monoclonal antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8:539-546.
   PubMed Web of Science ®Google Scholar
• Takagaki, Y., A. DeCloux, M. Bonneville, and S. Tonegawa. 1988. Diversity of γδ T-cell receptors on murine intestinal intraepithelial lymphocytes. Nature (London) 339:712-714.
   Google Scholar
• Takagaki, Y., N. Nakanishi, I. Ishida, O. Kanagawa, and S. Tonegawa. 1989. Τ cell receptor-γ and -δ genes preferentially utilized by adult thymocytes for the surface expression. J? Immunol. 142:2112.
   Google Scholar
• Tomonari, K. 1988. Rat antibody against a structure functionally related to the mouse Τ cell receptor-T3 complex. Immunoge-netics 28:445-458.
   PubMed Web of Science ®Google Scholar
• Viney, J. L., Τ. Τ. MacDonald, and P. J. Kilshaw. 1989. T-cell receptor expression in intestinal intra-epithelial lymphocyte subpopulations of normal and athymic mice. Immunology 66:583-587.
   PubMedGoogle Scholar
• von Boehmer, H., and H. Kisielow. 1990. Self-nonself discrimination by Τ cells. Science 248:1369-1373.
   PubMedGoogle Scholar
• Wilson, R. K., C. Chen, and L. Hood. 1990. Optimization of asymmetric polymerase chain reaction for rapid fluorescent DNA sequencing. BioTechniques 8:184-189.
   PubMed Web of Science ®Google Scholar
• Yoshikai, Y., G. Matsuzuki, Y. Takeda, S. Ohga, K. Kishihara, H. Yuuki, and K. Nomoto. 1988. Functional Τ cell receptor delta chain gene messages in athymic nude mice. Eur. J. Immunol. 18:1039-1043.
   PubMedGoogle Scholar
• Yoshikai, Y., M. D. Reis, and T. W. Mak. 1986. Athymic mice express a high level of functional γ-chain but greatly reduced levels of a- and β-chain T-cell receptor messages. Nature (London) 324:482-485.
   PubMedGoogle Scholar
• Zinkernagel, R. M., G. N. Callahan, A. Althage, S. Cooper, P. A. Klein, and J. Klein. 1978. On the thymus in the differentiation of "H-2 self-recognition" by Τ cells: evidence for dual recognition? J. Exp. Med. 147:882-895.
   PubMed Web of Science ®Google Scholar