Dissection of the Mouse N-ras Gene Upstream Regulatory Sequences and Identification of the Promoter and a Negative Regulatory Element

REFERENCES

• Baniahmad, A., M. Muller, C. Steiner, and R. Renkawitz. 1987. Activity of two different silencer elements of the chicken lysozyme can be compensated by enhancer elements. EMBO J. 6:2297-2303.
   PubMedGoogle Scholar
• Barbacid, M. 1987. ras genes. Annu. Rev. Biochem. 56:779-827.
   PubMed Web of Science ®Google Scholar
• Bar-Sagi, D., and J. R. Feramisco. 1985. Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. Cell 42:841-848.
   PubMed Web of Science ®Google Scholar
• Bond, V. C., and B. Wold. 1987. Poly-L-ornithine-mediated transformation of mammalian cells. Mol. Cell. Biol. 7:2286-2293.
   PubMed Web of Science ®Google Scholar
• Brand, A. H., L. Breeden, J. Abraham, R. Sternglanz, and K. Nasmyth. 1985. Characterization of a silencer in yeasts: a DNA sequence with properties opposite to those of a transcriptional enhancer. Cell 41:41-48.
   PubMed Web of Science ®Google Scholar
• Carthew, R. W., L. A. Chodosh, and P. A. Sharp. 1985. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell 43:439-448.
   PubMed Web of Science ®Google Scholar
• Chang, E. H., M. E. Furth, E. M. Scolnick, and D. R. Lowy. 1982. Tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of Harvey murine sarcoma virus. Nature (London) 297:479-483.
   PubMed Web of Science ®Google Scholar
• Chang, H. Y., I. Guerrero, R. Lake, A. Pellicer, and P. D’Eustachio. 1987. Mouse N-ras genes: organization of the functional locus and of a truncated cDNA-like pseudogene. Oncogene Res. 1:129-136.
   PubMedGoogle Scholar
• Chirgwin, J. M., A. B. Przybyla, R. J. McDonald, and W. J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299.
   PubMed Web of Science ®Google Scholar
• Chiswell, D. J., D. A. Gillespie, and J. A. Wyke. 1982. The changes in proviral chromatin that accompany morphological variation in avian sarcoma virus-infected cells. Nucleic Acids Res. 10:3967-3980.
   PubMedGoogle Scholar
• Curatola, A. Personal communication.
   Google Scholar
• Dignam, J. D., R. M. Lebovitz, and R. G. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from mammalian nuclei. Nucleic Acids Res. 11:1475-1489.
   PubMed Web of Science ®Google Scholar
• Doniger, J., and J. A. Di Paolo. 1988. Coordinate N-ras mRNA up-regulation with mutational activation in tumorigenic guinea pig cells. Nucleic Acids Res. 16:969-980.
   PubMedGoogle Scholar
• Feramisco, J. R., M. Gross, T. Kamata, M. Rosenberg, and R. W. Sweet. 1984. Microinjection of the oncogene form of the human H-ras (T-24) protein results in rapid proliferation of quiescent cells. Cell 38:109-111.
   PubMed Web of Science ®Google Scholar
• Goodbourn, S., and T. Maniatis. 1988. Overlapping positive and negative regulatory domains of the human beta-interferon gene. Proc. Natl. Acad. Sci. USA 85:1447-1451.
   PubMedGoogle Scholar
• Gorman, C. M., L. F. Moffat, and B. H. Howard. 1982. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2:1044-1051.
   PubMed Web of Science ®Google Scholar
• Gross, D. S., and W. T. Garrard. 1988. Nuclease hypersensitive sites in chromatin. Annu. Rev. Biochem. 57:159-197.
   PubMed Web of Science ®Google Scholar
• Guerrero, I., P. Calzada, A. Mayer, and A. Pellicer. 1984. A molecular approach to leukemogenesis: mouse lymphomas contain an activated c-ras oncogene. Proc. Natl. Acad. Sci. USA 81:202-205.
   PubMed Web of Science ®Google Scholar
• Guerrero, I., A. Villasante, V. Corces, and A. Pellicer. 1985. Loss of the normal N-ras allele in a mouse thymic lymphoma induced by a chemical carcinogen. Proc. Natl. Acad. Sci. USA 82:7810-7814.
   PubMed Web of Science ®Google Scholar
• Guerrero, I., H. Wong, A. Pellicer, and D. E. Burstein. 1986. Activated N-ras induces neuronal differentiation of PC12 rat pheochromocytoma cells. J. Cell. Physiol. 129:71-76.
   PubMedGoogle Scholar
• Hall, A., and R. Brown. 1985. Human N-ras: cDNA cloning and gene structure. Nucleic Acids Res. 13:5255-5268.
   PubMedGoogle Scholar
• Hall, V. C., P. E. Jacob, G. M. Ringold, and F. Lee. 1983. Expression and regulation of E. coli lac Z gene fusions in mammalian cells. J. Mol. Appl. Genet. 2:101-109.
   PubMedGoogle Scholar
• Henikoff, S. 1984. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351-359.
   PubMed Web of Science ®Google Scholar
• Herbomel, P., B. Bourachot, and M. Yaniv. 1984. Two distinct enhancers with different cell specificity coexist in the regulatory region of polyoma. Cell 39:653-662.
   PubMed Web of Science ®Google Scholar
• Herbst, R. S., E. M. Boezko, J. E. Darnell, and L. E. Babiss. 1990. The mouse albumin enhancer contains a negative regulatory element that interacts with a novel DNA-binding protein. Mol. Cell. Biol. 10:3896-3905.
   PubMedGoogle Scholar
• Hoffman, E. K., S. P. Trusko, N. Freeman, and D. L. George. 1987. Structural and functional characterization of the promoter region of mouse c-K-ras gene. Mol. Cell. Biol. 7:2592-2596.
   PubMed Web of Science ®Google Scholar
• Honkawa, H., W. Masahashi, S. Hashimoto, and T. Hashimoto-Gotoh. 1987. Identification of the principal promoter sequence of the c-H-ras transforming oncogene: deletion analysis of the 5′ flanking region by focus formation assay. Mol. Cell. Biol. 7:2933-2940.
   PubMedGoogle Scholar
• Ishii, S., J. T. Kadonaga, R. Tjian, J. N. Brady, G. T. Merlino, and I. Pastan. 1986. Binding of the Sp1 transcription factor by the human Harvey ras1 proto-oncogene promoter. Science 232:1410-1413.
   PubMed Web of Science ®Google Scholar
• Jeffers, M., R. Paciucci, and A. Pellicer. 1990. Characterization of unr; a gene closely linked to N-ras. Nucleic Acids Res. 18:4891-4899.
   PubMedGoogle Scholar
• Kadonaga, J. T., K. A. Jones, and R. Tjian. 1986. Promoter specific activation of RNA polymerase II transcription by Sp1. Trends Biochem. Sci. 11:20-23.
   Web of Science ®Google Scholar
• Lee, W., P. Mitchell, and R. Tjian. 1987. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements. Cell 49:741-752.
   PubMed Web of Science ®Google Scholar
• Leon, J., I. Guerrero, and A. Pellicer. 1987. Differential expression of the ras gene family in mice. Mol. Cell. Biol. 7:1535-1540.
   PubMed Web of Science ®Google Scholar
• Levine, M., and J. L. Manley. 1989. Transcriptional repression of eukaryotic promoters. Cell 59:405-408.
   PubMedGoogle Scholar
• Luckow, B., and G. Schutz. 1987. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15:5490.
   PubMed Web of Science ®Google Scholar
• McKay, I. A., C. J. Marshall, C. Cales, and A. Hall. 1986. Transformation and stimulation of DNA synthesis in NIH 3T3 cells are a titratable function of normal p21 N-ras expression. EMBO J. 5:2617-2621.
   PubMedGoogle Scholar
• Melton, D. A., P. A. Krieg, M. R. Rebagliati, T. Maniatis, K. Zinn, and M. R. Green. 1984. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12:7035-7070.
   PubMed Web of Science ®Google Scholar
• Mitchell, P. J., C. Wang, and R. Tjian. 1987. Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell 50:847-861.
   PubMed Web of Science ®Google Scholar
• Muglia, A., and L. B. Rothman-Denes. 1986. Cell type-specific negative regulatory element in the control region of the rat α-fetoprotein gene. Proc. Natl. Acad. Sci. USA 83:7653-7657.
   PubMed Web of Science ®Google Scholar
• Mulcahy, L. S., M. R. Smith, and D. W. Stacey. 1985. Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells. Nature (London) 313:241-243.
   PubMed Web of Science ®Google Scholar
• Noda, M., M. Ko, A. Ogura, D. G. Liu, T. Anano, T. Takano, and Y. Ikawa. 1985. Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line. Nature (London) 318:73-75.
   PubMed Web of Science ®Google Scholar
• Paciucci, R. Unpublished data.
   Google Scholar
• Propst, F., P. M. Rosenberg, and G. F. Van Woude. 1988. Protooncogene expression in germ cell development. Trends Genet. 4:183-187.
   PubMedGoogle Scholar
• Pulciani, S., E. Santos, L. Long, V. Sorrentino, and M. Barbacid. 1985. ras gene amplification and malignant transformation. Mol. Cell. Biol. 5:2836-2841.
   PubMed Web of Science ®Google Scholar
• Remmers, E. F., J. Q. Yang, and K. B. Marcu. 1986. A negative transcriptional control element located upstream of the murine c-myc gene. EMBO J. 5:899-904.
   PubMed Web of Science ®Google Scholar
• Ricketts, M. H., and A. D. Levinson. 1988. High-level expression of c-H-ras1 gene fails to fully transform rat-1 cell. Mol. Cell. Biol. 8:1460-1468.
   PubMedGoogle Scholar
• Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Schaeffer, E., F. Boissier, M. D. Py, G. N. Cohen, and M. M. Zakin. 1989. Cell type-specific expression of the human transferrin gene. J. Biol. Chem. 264:7153-7160.
   PubMed Web of Science ®Google Scholar
• Schwab, M., K. Alitalo, H. E. Varmus, J. M. Bishop, and D. George. 1983. A cellular oncogene (c-K-ras) is amplified, overexpressed and located within karyotypic abnormalities in mouse adrenocortical tumor cells. Nature (London) 303:497-501.
   PubMed Web of Science ®Google Scholar
• Sorrentino, V., M. D. McKinney, M. Giorgi, R. Geremia, and E. Fleissner. 1988. Expression of cellular protooncogenes in the mouse male germ line: a distinctive 2.4 kilobase pim-1 transcript is expressed in aploid post-meiotic cells. Proc. Natl. Acad. Sci. USA 85:2191-2195.
   PubMedGoogle Scholar
• Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
   PubMed Web of Science ®Google Scholar
• Subramaniam, A., J. Gulick, and J. Robbins. 1990. Analysis of the upstream regulatory region of a chicken skeletal myosin heavy chain gene. J. Biol. Chem. 265:13986-13994.
   PubMedGoogle Scholar
• Winoto, A., and D. Baltimore. 1989. αβ Lineage-specific expression of the αT cell receptor gene by nearby silencers. Cell 59:649-655.
   PubMedGoogle Scholar
• Wu, C. 1980. The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature (London) 286:854-860.
   PubMed Web of Science ®Google Scholar
• Yamamoto, F., and M. Perucho. 1988. Characterization of the human c-K-ras gene promoter. Oncogene Res. 3:125-138.
   PubMedGoogle Scholar