Identification of a cis-Acting Sequence Required for Germ LineSpecific Splicing of the P Element ORF2-ORF3 Intron

REFERENCES

• Ashburner, M. 1989. Drosophila—a laboratory handbook, p. 969-1015. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Bingham, P., T. B. Chou, I. Mims, and Z. Zachar. 1988. On/off regulation of gene expression at the level of splicing. Trends Genet. 4:134-138.
   PubMed Web of Science ®Google Scholar
• Chain, A. Unpublished data.
   Google Scholar
• Dignam, J. D., R. M. Lebovitz, and R. G. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475-1489.
   PubMed Web of Science ®Google Scholar
• Engels, W. R. 1983. The P family of transposable elements in Drosophila. Annu. Rev. Genet. 17:315-344.
   PubMed Web of Science ®Google Scholar
• Engels, W. R. 1984. A trans-acting product needed for P factor transposition in Drosophila. Science 226:1194-1196.
   PubMedGoogle Scholar
• Engels, W. R. 1989. P elements in Drosophila melanogaster, p. 437-484. In D. E. Berg and M. M. Howe (ed.), Mobile DNA. American Society for Microbiology, Washington, D.C.
   Google Scholar
• Engels, W. R., W. K. Benz, C. R. Preston, P. L. Graham, R. W. Phillis, and H. M. Robertson. 1987. Somatic effects of P element activity in Drosophila melanogaster: pupal lethality. Genetics 117:745-757.
   PubMed Web of Science ®Google Scholar
• Karess, R. E., and G. M. Rubin. 1984. Analysis of P transposable element functions in Drosophila. Cell 38:135-146.
   PubMed Web of Science ®Google Scholar
• Kawasaki, E. S. 1990. Amplification of RNA, p. 21-27. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (ed.), PCR protocols: a guide to methods and applications. Academic Press, Inc., San Diego, Calif.
   Google Scholar
• Keller, E. B., and W. A. Noon. 1985. Intron splicing: a conserved internal signal in introns of Drosophila melanogaster. Nucleic Acids Res. 13:4971-4981.
   PubMed Web of Science ®Google Scholar
• Kidwell, M. G., J. F. Kidwell, and J. A. Sved. 1977. Hybrid dysgenesis in Drosophila melanogaster: a syndrome of aberrant traits including mutation, sterility, and male recombination. Genetics 36:813-83.
   Google Scholar
• Laski, F. Unpublished data.
   Google Scholar
• Laski, F. A., D. C. Rio, and G. M. Rubin. 1986. Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing. Cell 44:7-19.
   PubMed Web of Science ®Google Scholar
• Laski, F. A., and G. M. Rubin. 1989. Analysis of the cis-acting requirements for germ-line specific splicing of the P-element ORF2-ORF3 intron. Genes Dev. 3:720-728.
   PubMedGoogle Scholar
• Misra, S., and D. C. Rio. 1990. Cytotype control of Drosophila P element transposition: the 66kd protein is a repressor of transposase activity. Cell 62:269-284.
   PubMedGoogle Scholar
• Moore, C. L., J. Chen, and J. Whoriskey. 1988. Two proteins crosslinked to RNA containing the adenovirus J3 poly(A) site require the AAUAAA sequence for binding. EMBO J. 7:3159-3169.
   PubMed Web of Science ®Google Scholar
• Mount, S. M., and J. A. Steitz. 1984. RNA splicing and the involvement of small ribonucleoproteins. Mod. Cell Biol. 3:249-297.
   Google Scholar
• O’Hare, K., and G. M. Rubin. 1983. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell 34:25-35.
   PubMed Web of Science ®Google Scholar
• Pelham, H. R. B. 1983. A regulatory upstream promoter element in the Drosophila hsp70 heat-shock gene. Cell 30:517-528.
   Google Scholar
• Reed, R., and T. Maniatis. 1986. A role for exon sequences and splice-site proximity in splice-site selection. Cell 46:681-690.
   PubMed Web of Science ®Google Scholar
• Rio, D. C., F. A. Laski, and G. M. Rubin. 1986. Identification and immunochemical analysis of biologically active Drosophila P element transposase. Cell 44:21-32.
   PubMed Web of Science ®Google Scholar
• Robertson, H. M., and W. R. Engels. 1989. Modified P elements that mimic the P cytotype in Drosophila melanogaster. Genetics 123:815-824.
   PubMedGoogle Scholar
• Roiha, H., G. M. Rubin, and K. O’Hare. 1988. P element insertions and rearrangements at the singed locus of Drosophila melanogaster. Genetics 119:75-83.
   PubMed Web of Science ®Google Scholar
• Siebel, C. W., and D. C. Rio. 1990. Regulated splicing of the Drosophila P transposable element third intron in vitro: somatic repression. Science 248:1200-1208.
   PubMedGoogle Scholar
• Yost, H. J., and S. Lindquist. 1986. RNA splicing is interrupted by heat shock and is rescued by heat shock protein synthesis. Cell 45:185-193.
   PubMed Web of Science ®Google Scholar