Introduction of Exogenous DNA into Chlamydomonas reinhardtii by Electroporation

REFERENCES

• Bandziulis, R., and J. Rosenbaum. 1988. Novel control elements in the alpha-1 tubulin gene promoter from Chlamydomonas reinhardii. Mol. Gen. Genet. 214:204–212.
   PubMedGoogle Scholar
• Boynton, J., N. Gillham, E. Harris, J. Hosier, A. Johnson, A. Jones, B. Randolph-Anderson, D. Robertson, T. Klein, K. Shark, and J. Sanford. 1988. Chloroplast transformation in Chlamydomonas with high-velocity microprojectiles. Science 240:15341538.
   Google Scholar
• Callis, J., M. Fromm, and V. Walbot. 1987. Expression of mRNA electroported into plant and animal cells. Nucleic Acids Res. 15:5823–5831.
   PubMedGoogle Scholar
• Davies, D., and A. Plaskitt. 1971. Genetic and structural analysis of cell wall formation in Chlamydomonas reinhardi. Genet. Res. 17:33–43.
   Google Scholar
• Debuchy, R., S. Purton, and J.-D. Rochaix. 1989. The arginino- succinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG 7 locus. EMBO J. 8:2803–2809.
   PubMedGoogle Scholar
• Deiner, D., A. Curry, K. Johnson, B. Williams, P. Lefebvre, K. Kindle, and J. Rosenbaum. 1990. Rescue of a paralyzed flagella mutant of Chlamydomonas reinhardtii by transformation. Proc. Natl. Acad. Sci. USA 87:5739–5743.
   PubMedGoogle Scholar
• Fromm, M., L. Taylor, and V. Walbot. 1985. Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc. Natl. Acad. Sci. USA 82:5824–5828.
   PubMed Web of Science ®Google Scholar
• Hasnein, S., E. Manavathu, and W.-C. Leung. 1985. DNA- mediated transformation of Chlamydomonas reinhardi cells: use of aminoglycoside 3′-phosphotransferase as a selectable marker. Mol. Cell. Biol. 5:3647–3650.
   PubMedGoogle Scholar
• Keller, L., J. Schloss, C. Silflow, and J. Rosenbaum. 1984. Transcription of alpha- and beta-tubulin genes in vitro in isolated Chlamydomonas reinhardi nuclei. J. Cell Biol. 98:1138–1143.
   PubMedGoogle Scholar
• Kindle, K. 1990. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 87:1228–1232.
   PubMed Web of Science ®Google Scholar
• Kindle, K., R. Schnell, E. Fernandez, and P. Lefebvre. 1989. Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J. Cell Biol. 109:2589–2601.
   PubMed Web of Science ®Google Scholar
• Knight, D., and M. Scrutton. 1986. Gaining access to the cytosol: the technique and some applications of electroperme- abilization. Biochem. J. 234:497–506.
   PubMed Web of Science ®Google Scholar
• Merhle, W., U. Zimmermann, and R. Hampp. 1985. Evidence for asymmetrical uptake of fluorescent dyes through electro- permeabilized membranes of Avena mesophyll protoplasts. FEBS Lett. 185:89–94.
   Google Scholar
• Morris, R., L. Keller, A. Zweidler, and P. Rizzo. 1990. Analysis of Chlamydomonas reinhardtii histones and chromatin. J. Pro- tozool. 37:117–123.
   Google Scholar
• Neumann, E., M. Schaefer-Ridder, Y. Wang, and P. Hofschneider. 1982. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1:841–845.
   PubMed Web of Science ®Google Scholar
• Rochaix, J.-D., and J. van Dillewijn. 1982. Transformation of the green alga Chlamydomonas reinhardii with yeast DNA. Nature (London) 296:70–72.
   PubMed Web of Science ®Google Scholar
• Sager, R., and S. Granick. 1953. Nutritional studies with Chlamydomonas reinhardi. Ann. N.Y. Acad. Sci. 56:831–838.
   PubMed Web of Science ®Google Scholar
• Schloss, J., C. Silflow, and J. Rosenbaum. 1984. mRNA abundance changes during flagellar regeneration in Chlamydomonas reinhardtii. Mol. Cell. Biol. 4:424—434.
   PubMedGoogle Scholar
• Sokoloski, J., M. Jastreboff, J. Berino, A. Sartorelli, and R. Narayanan. 1986. Introduction of deoxyribonucleic triphosphates into intact cells by electroporation. Anal. Biochem. 158:272–277.
   PubMedGoogle Scholar
• Widholm, J. 1972. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Tech. 47:189–194.
   PubMed Web of Science ®Google Scholar
• Winegar, R., and L. Lutze. 1990. Introduction of biologically active proteins into viable cells by electroporation. Focus 12:34–37.
   Google Scholar
• Zimmermann, U., G. Pilwat, and F. Riemann. 1975. Preparation of erythrocyte ghosts by dielectric breakdown of the cell membrane. Biochim. Biophys. Acta 375:209–219.
   PubMedGoogle Scholar