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Gene Expression

Mutations in the Yeast RNA14 and RNA15 Genes Result in an Abnormal mRNA Decay Rate; Sequence Analysis Reveals an RNA-Binding Domain in the RNA15 Protein

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Pages 3075-3087 | Received 15 Oct 1990, Accepted 13 Mar 1991, Published online: 01 Apr 2023

References

  • Adam, S. A., T. Nakagawa, M. S. Swanson, T. K. Woodruff, and G. Dreyfuss. 1986. mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence. Mol. Cell. Biol. 6:2932-2943.
  • Ahlquist, P., and P. Kaesberg. 1979. Determination of the length distribution of poly(A) at the 3′ terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, Rav-61 and CPMV and of mouse globin mRNA. Nucleic Acids Res. 7:1195-1204.
  • Amrein, H., M. Gorman, and R. Nöthiger. 1988. The sex-determining gene tra-2 of Drosophila encodes a putative RNA binding protein. Cell 55:1025-1035.
  • Atkinson, N. S., R. W. Dunst, and A. K. Hopper. 1985. Characterization of an essential Saccharomyces cerevisiae gene related to RNA processing: cloning of RNA1 and generation of a new allele with a novel phenotype. Mol. Cell. Biol. 5:907-915.
  • Bach, M. L., F. Lacroute, and D. Botstein. 1979. Evidence for transcriptional regulation of orotidine-5′-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli. Proc. Natl. Acad. Sci. USA 76:386-390.
  • Bandziulis, R. J., M. S. Swanson, and G. Dreyfuss. 1989. RNA-binding proteins as developmental regulators. Genes Dev. 3:431-437.
  • Banroques, J., A. Delahodde, and C. Jacq. 1986. A mitochondrial RNA maturase gene transferred to the yeast nucleus can control mitochondrial mRNA splicing. Cell 46:837-844.
  • Beggs, J. D. 1978. Transformation of yeast by replicating hybrid Plasmid. Nature (London) 275:104-109.
  • Bell, L. R., E. M. Maine, P. Schedl, and T. W. Cline. 1988. Sex-lethal, a Drosophila sex determination switch gene, exhibits sex-specificity RNA splicing and sequence similarity to RNA binding proteins. Cell 55:1037-1046.
  • Bennetzen, J. L., and B. D. Hall. 1982. Codon selection in yeast. J. Biol. Chem. 257:3026-3031.
  • Bernstein, P., S. W. Peltz, and J. Ross. 1989. The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro. Mol. Cell. Biol. 9:659-670.
  • Bernstein, P., and J. Ross. 1989. Poly(A), poly(A) binding protein and the regulation of mRNA stability. Trends Biochem. Sci. 14:373-377.
  • Bloch, J. C., F. Perrin, and F. Lacroute. 1978. Yeast temperature-sensitive mutants impaired in processing of poly(A)-containing RNAs. Mol. Gen. Genet. 165:123-127.
  • Bolivar, F., R. L. Rodriguez, P. J. Greene, M. C. Betlach, H. L. Heyneker, M. W. Boyer, J. H. Crosa, and S. Falkow. 1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95-113.
  • Borer, R. A., C. F. Lehner, H. M. Eppenberger, and E. A. Nigg. 1989. Major nucleolar proteins shuttle between nucleus and cytoplasm. Cell 56:379-390.
  • Botstein, D., and R. W. Davis. 1982. Principles and practice of recombinant DNA research with yeast, p. 607-636. In J. N. Strathern, E. W. Jones, and J. R. Broach (ed.), The molecular biology of the yeast Saccharomyces: metabolism and gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Brewer, G., and J. Ross. 1988. Poly(A) shortening and degradation of the 3′-AU rich sequences of the human c-myc mRNA in a cell-free system. Mol. Cell. Biol. 8:1697-1708.
  • Butler, J. S., and T. Piatt. 1988. RNA processing generates the mature 3′ end of yeast CYC1 messenger RNA in vitro. Science 242:1270-1274.
  • Caizergues-Ferrer, M., P. Mariottini, C. Curie, B. Lapeyre, N. Gas, F. Amalric, and F. Amaldi. 1989. Nucleolin from Xenopus laevis: cDNA cloning and expression during development. Genes Dev. 3:324-333.
  • Carle, G. F., and M. V. Olson. 1985. An electrophoretic karyotype for yeast. Proc. Natl. Acad. Sci. USA 82:3756-3760.
  • Chevallier, M. R., J. C. Bloch, and F. Lacroute. 1980. Transcriptional and translational expression of a chimeric bacterial-yeast Plasmid in yeasts. Gene 11:11-19.
  • Chia, L. L., and C. McLaughlin. 1979. The half-life of mRNA in Saccharomyces cerevisiae. Mol. Gen. Genet. 170:137-144.
  • Chu, G., D. Vollrath, and R. W. Davis. 1986. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234:1582-1585.
  • Cigan, A. M., and T. F. Donahue. 1987. Sequence and structural features associated with translational initiator regions in yeast—a review. Gene 59:1-18.
  • Cobianchi, F., D. N. SenGupta, B. Z. Zmudzka, and S. H. Wilson. 1986. Structure of rodent helix-destabilizing protein revealed by cDNA cloning. J. Biol. Chem. 261:3536-3543.
  • Creusot, F., J. Verdière, M. Gaisne, and P. P. Slonimski. 1988. CYP1 (HAP1) regulator of oxygen-dependent gene expression in yeast. I. Overall organization of the protein sequence displays several novel structural domains. J. Mol. Biol. 204:263-276.
  • Davis, R. W., M. Thomas, J. Cameron, T. P. St. John, S. Sherer, and R. A. Padgett. 1980. Rapid DNA isolation for enzymatic and hybridization analysis. Methods Enzymol. 65:404-411.
  • Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
  • Diffley, J. F. X., and B. Stillman. 1989. Similarity between the transcriptional silencer binding proteins ABF1 and RAP1. Science 246:1034-1038.
  • Drummond, D. R., J. Armstrong, and A. Colman. 1985. The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes. Nucleic Acids Res. 13:7375-7394.
  • Etzerodt, M., R. Vignali, G. Ciliberto, D. Scherly, I. W. Mattaj, and L. Philipson. 1988. Structure and expression of a Xenopus gene encoding an snRNP protein (Ul 70K). EMBO J. 7:4311-4321.
  • Feinberg, A. P., and B. Vogelstein. 1984. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
  • Fogel, S., R. K. Mortimer, and K. Lusnak. 1981. Mechanisms of meiotic gene conversion in yeast, or “wanderings on a foreign strand”, p. 289-339. In J. N. Strathern, E. W. Jones, and J. R. Broach (ed.), The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Frigerio, G., B. Maya, D. Bopp, S. Baumgartner, and M. Noll. 1986. Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network. Cell 47:735-746.
  • Gallwitz, D., C. Donath, and C. Sander. 1983. A yeast gene encoding a protein homologous to the human c-haslbas proto-oncogene product. Nature (London) 306:704-707.
  • Gómez, J., D. Sánchez-Martínez, V. Stiefel, J. Rigau, P. Puigdomènech, and M. Pagès. 1988. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature (London) 334:262-264.
  • Grange, T., C. Martins de Sa, J. Oddos, and R. Pictet. 1987. Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif. Nucleic Acids Res. 15:4771-4787.
  • Habets, W. J., P. T. G. Sillekens, M. H. Hoet, J. A. Schalken, A. J. M. Roebroek, J. A. M. Leunissen, W. J. M. van de Ven, and W. J. Venrooij. 1987. Analysis of a cDNA clone expressing a human autoimmune antigen: full-length sequence of the U2 small nuclear RNA-associated B” antigen. Proc. Natl. Acad. Sci. USA 84:2421-2425.
  • Hamilton, R., C. K. Watanabe, and H. A. de Boer. 1987. Compilation and comparison of the sequence context around the AUG start codon in Saccharomyces cerevisiae mRNAs. Nucleic Acids Res. 15:3581-3593.
  • Haynes, S. R., M. L. Rebbert, B. A. Mozer, F. Forquignon, and I. B. Dawid. 1987. pen repeat sequences are GGN clusters and encode a glycine-rich domain in a Drosophila cDNA homologous to the rat helix destabilizing protein. Proc. Natl. Acad. Sci. USA 84:1819-1823.
  • Herrick, D., R. Parker, and A. Jacobson. 1990. Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae. Mol. Cell. Biol. 10:2269-2284.
  • Higgins, D. G., and P. M. Sharp. 1988. CLUSTAL: a package for performing multiple sequence alignements on a microcomputer. Gene 73:237-244.
  • Hitzeman, R. A., F. E. Hagie, J. S. Hayflick, C. Y. Chen, P. H. Seeburg, and R. Derynck. 1982. The primary structure of the Saccharomyces cerevisiae gene for 3-phosphoglycerate kinase. Nucleic Acids Res. 10:7791-7808.
  • Huez, G., G. Marbaix, D. Gallwitz, E. Weinberg, R. Devos, E. Hubert, and Y. Cleuter. 1978. Functional stabilisation of HeLa cell histone messenger RNAs injected into Xenopus oocytes by 3′-OH polyadenylation. Nature (London) 271:572-573.
  • Hutchison, H. T., L. H. Hartwell, and C. S. McLaughlin. 1969. Temperature-sensitive yeast mutant defective in ribonucleic acid production. J. Bacteriol. 99:807-814.
  • Jackson, R. J., and N. Standart. 1990. Do the poly(A) tail and 3′ untranslated region control mRNA translation? Cell 62:15-24.
  • Jacobson, A., and M. Favreau. 1983. Possible involvement of poly(A) in protein synthesis. Nucleic Acids Res. 11:6353-6368.
  • Jimenez, A., D. J. Tipper, and J. Davies. 1973. Mode of action of thiolutin, an inhibitor of macromolecular synthesis in Saccharomyces cerevisiae. Antimicrob. Agents Chemother. 3:729-738.
  • Krowczynska, A., R. Yenofski, and G. Brawerman. 1985. Regulation of messenger RNA stability in mouse erythroleukemia cells. J. Mol. Biol. 181:231-239.
  • Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
  • Lacroute, F. Unpublished data.
  • Lahiri, D. K., and J. O. Thomas. 1986. A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2. Nucleic Acids Res. 14:4077-4094.
  • Langford, C. J., F. J. Klinz, C. Donath, and D. Gallwitz. 1984. Point mutations identify the conserved, intron-contained TAC TAAC box as an essential sequence in yeast. Cell 36:645-653.
  • Lapeyre, B., H. Bourbon, and F. Amalric. 1987. Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence. Proc. Natl. Acad. Sci. USA 84:1472-1476.
  • Marek, C. 1988. ‘DNA Strider’: A ‘C’ program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucleic Acids Res. 16:1829-1836.
  • Mayer, S. A., and C. L. Dieckmann. 1989. The yeast CBP1 gene produces two differentially regulated transcripts by alternative 3′-end formation. Mol. Cell. Biol. 9:4161-4169.
  • Milbrandt, J. 1987. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor. Science 238:797-799.
  • Moore, C. L., and P. A. Sharp. 1984. Site-specific polyadenylation in a cell-free reaction. Cell 36:581-591.
  • Mortimer, R. K., and D. C. Hawthorne. 1966. Genetic mapping in Saccharomyces cerevisiae. Genetics 53:165-173.
  • Munroe, D., and A. Jacobson. 1990. mRNA poly(A) tail, a 3′ enhancer of translational initiation. Mol. Cell. Biol. 10:3441-3455.
  • Nonet, M., C. Scafe, J. Sexton, and R. Young. 1987. Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis. Mol. Cell. Biol. 7:1602-1611.
  • O’Hara, P. J., H. Horowitz, G. Heichinger, and E. T. Young. 1988. The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain. Nucleic Acids Res. 16:10153-10169.
  • Orr-Weaver, T. L., J. W. Szostak, and R. J. Rothstein. 1981. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. USA 78:6354-6358.
  • Palatnik, C. M., C. Wilkins, and A. Jacobson. 1984. Translation control during early Dictyostelium development: possible involvement of poly(A) sequences. Cell 36:1017-1025.
  • Passmore, S., G. T. Maine, R. Elble, C. Christ, and B.-K. Tye. 1988. Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MATa cells. J. Mol. Biol. 204:593-606.
  • Pearson, W. R., and D. J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444-2448.
  • Piper, P. W., and J. L. Aamand. 1989. Yeast mutation thought to arrest mRNA transport markedly increases the length of the 3′ poly(A) on polyadenylated RNA. J. Mol. Biol. 208:697-700.
  • Regulski, M., K. Harding, R. Kostriken, F. Karch, M. Levine, and W. McGinnis. 1985. Homeo box genes of the Antennapedia and Bithorax complexes of Drosophila. Cell 43:71-80.
  • Sachs, A. B., M. W. Bond, and R. D. Kornberg. 1986. A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression. Cell 45:827-835.
  • Sachs, A. B., and R. W. Davis. 1989. The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation. Cell 58:857-867.
  • Sachs, A. B., R. W. Davis, and R. D. Kornberg. 1987. A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. Mol. Cell. Biol. 7:3268-3276.
  • Sanger, F., S. Nicklen, and A. R. Coulsen. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
  • Shapiro, R. A., D. Herrick, R. E. Manrow, D. Blinder, and A. Jacobson. 1988. Determinants of mRNA stability in Dictyostelium discoideum amoebae: differences in poly(A) tail length, ribosome loading, and mRNA size cannot account for the heterogeneity of mRNA decay rates. Mol. Cell. Biol. 8:1957-1969.
  • Sherman, F., G. R. Fink, and J. B. Hicks. 1986. Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Shiokawa, K., and A. O. Pogo. 1974. The role of cytoplasmic membranes in controlling the transport of nuclear messenger RNA and initiation of protein synthesis. Proc. Natl. Acad. Sci. USA 71:2658-2662.
  • Sillekens, P. T. G., R. P. Habets, R. P. Beijeir, and W. J. van Venrooij. 1987. cDNA cloning of the human Ul snRNA-associated A protein: extensive homology between Ul and U2 snRNP-specific proteins. EMBO J. 6:3841-3848.
  • Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
  • Srivastava, M., P. J. Fleming, H. B. Pollard, and A. Lee Burns. 1989. Cloning and sequencing of the human nucleolin cDNA. FEBS Lett. 250:99-105.
  • Theissen, H., M. Etzerodt, R. Reuter, C. Schneider, F. Lottspeich, P. Argos, R. Lührmann, and L. Philipson. 1986. Cloning of the human cDNA for the Ul RNA-associated 70K protein. EMBO J. 5:3209-3217.
  • Verdier, J.-M. 1990. Regulatory DNA-binding proteins in yeast: an overview. Yeast 6:271-297.
  • Wahlgren, M., L. Åslund, L. Franzén, M. Sundvail, B. Wahlin, K. Berzins, L. A. McNicol, A. Björkman, H. Wigzell, P. Perlman, and U. Pettersson. 1986. A Plasmodium falciparum antigen containing clusters of asparagine residues. Proc. Natl. Acad. Sci. USA 83:2677-2681.
  • Wharton, K. A., B. Yedvobnick, V. G. Finnerty, and S. Artavanis-Tsakonas. 1985. opa: a novel family of transcribed repeats shared by the notch locus and other developmentally regulated loci in D. melanogaster. Cell 40:55-62.
  • Wilson, M. C., S. G. Sawicki, P. A. White, and J. E. Darnell, Jr. 1978. A correlation between the rate of poly (A) shortening and half-life of messenger RNA in adenovirus transformed cells. J. Mol. Biol. 126:23-36.
  • Wilson, T., and R. Treisman. 1988. Removal of poly (A) and consequent degradation of c-fos mRNA facilitated by 3′ AUrich sequences. Nature (London) 336:396-399.
  • Woychik, N. A., and R. A. Young. 1989. RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth. Mol. Cell. Biol. 9:2854-2859.
  • Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103-119.
  • Zeevi, M., J. R. Nevins, and J. E. Darnell, Jr. 1982. Newly formed mRNA lacking polyadenylic acid enters the cytoplasm and the polyribosomes but has a shorter half-life in the absence of polyadenylic acid. Mol. Cell. Biol. 2:517-525.
  • Zelus, B. D., D. H. Giebelhaus, D. W. Eib, K. A. Kenner, and R. T. Moon. 1989. Expression of the poly(A)-binding protein during development of Xenopus laevis. Mol. Cell. Biol. 9:2756-2760.

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