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Cell Growth and Development

A ras Effector Domain Mutant Which Is Temperature Sensitive for Cellular Transformation: Interactions with GTPase-Activating Protein and NF-1

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Pages 3132-3138 | Received 26 Dec 1990, Accepted 25 Mar 1991, Published online: 01 Apr 2023

References

  • Adari, H., D. R. Lowy, B. M. Willumsen, C. J. Der, and F. McCormick. 1988. Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain. Science 240:518-521.
  • Ballester, R., D. Marchuk, M. Boguski, A. Saulino, R. Letcher, M. Wigler, and F. Collins. 1990. The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins. Cell 63:851-859.
  • Barbacid, M. 1987. ras genes. Annu. Rev. Biochem. 56:779-827.
  • Bos, J. L. 1988. The ras gene family and human carcinogenesis. Mutation Res. 195:255-271.
  • Bourne, H. R., D. A. Sanders, and F. McCormick. 1990. The GTPase superfamily: a conserved switch for diverse cell functions. Nature (London) 348:125-132.
  • Calés, C., J. F. Hancock, C. Marshall, and A. Hall. 1988. The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene product. Nature (London) 332:548-551.
  • Field, J., D. Broek, T. Kataoka, and M. Wigler. 1987. Guanine nucleotide activation of, and competition between, RAS proteins from Saccharomyces cerevisiae. Mol. Cell. Biol. 7:2128-2133.
  • Frech, M., J. John, V. Pizon, P. Chardin, A. Tavitian, R. Clark, F. McCormick, and A. Wittinghofer. 1990. Inhibition of GTPase activating protein stimulation of RAS-P21 GTPase by the Krev-1 gene product. Science 249:169-171.
  • Furth, M. E., L. J. Davis, B. Fleurdelys, and E. M. Scolnick. 1982. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43:294-304.
  • Gibbs, J., M. D. Schaber, M. S. Marshall, E. M. Scolnick, and I. S. Sigal. 1987. Identification of guanine nucleotides bound to ras-encoded proteins in growing yeast cells. J. Biol. Chem. 262:10426-10429.
  • Hall, A. 1990. The cellular functions of small GTP-binding proteins. Science 249:635-640.
  • Hart, P. A., and C. J. Marshall. 1990. Amino acid 61 is a determinant of sensitivity of RAP proteins to the RAS GTPase activating protein. Oncogene 5:1099-1101.
  • Hutchison, C. A., III, S. K. Nordeen, K. Vogt, and M. H. Edgell. 1986. A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus. Proc. Natl. Acad. Sci. USA 83:710-714.
  • Jhappan, C., G. P. Vande Woude, and T. Robins. 1986. Transduction of host cellular sequences by a retroviral shuttle vector. J. Virol. 60:750-753.
  • Lacal, J. C., E. Santos, V. Notario, M. Barbacid, S. Yamazaki, H.-F. Kung, C. Seamans, S. McAndrew, and R. Crowl. 1984. Expression of normal and transforming H-ras genes in Escherichia coli and purification of their encoded p21 proteins. Proc. Natl. Acad. Sci. USA 81:5305-5309.
  • Mann, R., R. C. Mulligan, and D. Baltimore. 1983. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33:153-159.
  • Martin, G. A., D. Viskochil, G. Bollag, P. C. McCabe, W. J. Crosier, H. Haubruck, L. Conroy, R. Clark, R. O’Connell, R. M. Cawthon, M. A. Innis, and F. McCormick. 1990. The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21. Cell 63:843-849.
  • Norrander, J., T. Kempe, and J. Messing. 1983. Construction of improved M13 vectors using oligonucleotide-directed mutagenesis. Gene 26:101-106.
  • Papageorge, A. G., D. R. Lowy, and E. M. Scolnick. 1982. Comparative biochemical properties of p21 ras molecules coded for by viral and cellular ras genes. J. Virol. 44:509-519.
  • Satoh, T., M. Endo, M. Nakafuku, S. Nakamura, and Y. Kaziro. 1990. Platelet-derived growth factor stimulates formation of active P21Ras. GTP complex in Swiss mouse 3T3-cells. Proc. Natl. Acad. Sci. USA 87:5993-5997.
  • Satoh, T., S. Nakamura, and Y. Kaziro. 1987. Induction of neurite formation in PC 12 cells by microinjection of proto-oncogenenic Ha-ras protein preincubated with guanosine-5′-O-(3-thiotriphosphate). Mol. Cell. Biol. 7:4553-4556.
  • Schaber, M. D., V. M. Garsky, D. Boylan, W. S. Hill, E. M. Scolnick, M. S. Marshall, I. S. Sigal, and J. S. Gibbs. 1989. Ras interaction with the GTPase-activating protein (GAP). Proteins Struct. Funct. Genet. 6:306-315.
  • Shih, T. Y., P. E. Stokes, G. W. Smythers, R. Dhar, and S. Oroszlan. 1982. Characterization of the phosphorylation sites and the surrounding aminoacid sequences of the p21 transforming proteins coded for by the Harvey and Kirsten strains of murine sarcoma viruses. J. Biol. Chem. 275:11767-11773.
  • Shih, T. Y., M. O. Weeks, H. A. Young, and E. M. Scolnick. 1970. p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for maintenance of transformation. J. Virol. 31:546-556.
  • Sigal, I. S., J. B. Gibbs, J. S. D’Alonzo, and E. M. Scolnick. 1986. Identification of effector residues and a neutralizing epitope of Ha-ras-encoded p21. Proc. Natl. Acad. Sci. USA 83:4725-4729.
  • Southern, J. P., and P. Berg. 1983. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promotor. J. Mol. Appl. Genet. 1:327-341.
  • Stone, J. C., W. C. Vass, B. M. Willumsen, and D. R. Lowy. 1988. p21-ras effector domain mutants constructed by “cassette” mutagenesis. Mol. Cell. Biol. 8:3565-3569.
  • Topp, W. C. 1981. Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113:408-411.
  • Trahey, M., and F. McCormick. 1987. A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants. Science 238:542-545.
  • Velu, T. J., W. C. Vass, D. R. Lowy, and P. E. Tambourin. 1989. Harvey murine sarcoma virus: influences of coding and noncoding sequences on cell transformation in vitro and oncogenicity in vivo. J. Virol. 63:1384-1392.
  • Vogel, U. S., R. A. F. Dixon, M. D. Schaber, R. E. Diehl, M. S. Marshall, E. M. Scolnick, I. S. Sigal, and J. B. Gibbs. 1988. Cloning of bovine GAP and its interaction with oncogenic ras p21. Nature (London) 335:90-93.
  • Willumsen, B. M., A. G. Papageorge, H.-F. Kung, K. Bekesi, T. Robins, M. Johnsen, W. C. Vass, and D. R. Lowy. 1986. Mutational analysis of a ras catalytic domain. Mol. Cell. Biol. 6:2646-2654.
  • Xu, G., B. Lin, K. Tanaka, D. Dunn, D. Wood, R. Gesteland, R. White, R. Weiss, and F. Tamanoi. 1990. The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae. Cell 63:835-841.
  • Yatani, A., K. Okabe, P. Polakis, R. Halenbeck, F. McCormick, and A. M. Brown. 1990. RAS P21 and Gap inhibit coupling of muscarinic receptors to atrial K+ channels. Cell 61:769-776.
  • Zhang, K., et al. Unpublished observations.
  • Zhang, K., J. E. DeClue, W. C. Vass, A. G. Papageorge, F. McCormick, and D. R. Lowy. 1990. Suppression of c-ras transformation by GTPase-activating protein. Nature (London) 346:754-756.
  • Zhang, K., M. Nöda, W. C. Vass, A. G. Papageorge, and D. R. Lowy. 1990. Identification of small clusters of divergent amino acids that mediate the opposing effects of RAS and Krev-l. Science 249:162-165.

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