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Cell Growth and Development

Interactions among the Subunits of the G Protein Involved in Saccharomyces cerevisiae Mating†

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Pages 1-8 | Received 27 Apr 1992, Accepted 05 Oct 1992, Published online: 01 Apr 2023

REFERENCES

  • Blinder, D., S. Bouvier, and D. D. Jenness. 1989. Constitutive mutants in the yeast pheromone response: ordered function of the gene products. Cell 56:479–486.
  • Blumer, K. J., and J. Thorner. 1990. β and γ subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the α subunit but are required for receptor coupling. Proc. Natl. Acad. Sci. USA 87:4363–4367.
  • Burkholder, A. C., and L. Hartwell. 1985. The yeast α-factor receptor: structural properties deduced from the sequence of the STE2 gene. Nucleic Acids Res. 13:8463–8475.
  • Chien, C., P. L. Bartel, R. Sternglanz, and S. Fields. 1991. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA 88:9578–9582.
  • Cole, G. Μ., D. E. Stone, and S. I. Reed. 1990. Stoichiometry of G protein subunits affects the Saccharomyces Cerevisiae mating pheromone signal transduction pathway. Mol. Cell. Biol. 10:510–517.
  • Cross, F., L. H. Hartwell, C. Jackson, and J. B. Konopka. 1988. Conjugation in Saccharomyces Cerevisiae. Annu. Rev. Cell Biol. 4:429–457.
  • Dang, C. V., J. Barrett, Μ. Villa-Garcia, L. Μ. S Resar, G. J. Kato, and E. R. Fearon. 1991. Intracellular leucine zipper interactions suggest c-myc hetero-oligomerization. Mol. Cell. Biol. 11:954–962.
  • Dietzel, C., and J. Kurjan. 1987. The yeast SCGl gene: a Gα-like protein implicated in the a- and α-factor response pathway. Cell 50:1001–1010.
  • Dietzel, C., and J. Kurjan. 1987. Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone. Mol. Cell. Biol. 7:4169–4177.
  • Fields, S., and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature (London) 340:245–246.
  • Finegold, A. A., W. R. Schafer, J. Rine, Μ. Whiteway, and F. Tamanoi. 1990. Common modifications of trimeric G proteins and ras protein: involvement of polyisoprenylation. Science 249:165–169.
  • Fung, B. K. 1983. Characterization of transduction from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits. J. Biol. Chem. 258:10495–10502.
  • Gill, G., and Μ. Ptashne. 1987. Mutants of GAL4 protein altered in an activation function. Cell 51:121–126.
  • Gilman, A. 1987. G proteins: transducers of receptor-generated signals. Annu. Rev. Biochem. 56:615–649.
  • Hagen, D. C., G. McCaffrey, and G. F. Sprague, Jr. 1986. Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a-factor: gene sequence and implications for the structure of the presumed receptor. Proc. Natl. Acad. Sci. USA 83:1418–1422.
  • Hartwell, L. H. 1980. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating pheromone. J. Cell Biol. 85:811–822.
  • Herskowitz, I. 1988. Life cycle of the budding yeast Saccharomyces cerevisiae. Microbiol. Rev. 52:536–553.
  • Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163–168.
  • Jahng, K.-Y., J. Ferguson, and S. I. Reed. 1988. Mutations in a gene encoding the α subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling. Mol. Cell. Biol. 8:2484–2493.
  • Jarvis, E. E., K. L. Clark, and G. F. Sprague, Jr. 1989. The yeast transcription activator PRTF, a homolog of the mamma-lian serum response factor, is encoded by the MCMl gene. Genes Dev. 3:936–945.
  • Jarvis, E. E., D. C. Hagen, and G. F. Sprague, Jr. 1988. Identification of a DNA segment that is necessary and sufficient for α-specific gene control in Saccharomyces cerevisiae: implications for regulation of α-specific and a-specific genes. Mol. Cell. Biol. 8:309–320.
  • Kalderon, D., B. L. Roberts, W. D. Richardson, and A. E. Smith. 1984. A short amino acid sequence able to specify nuclear location. Cell 39:499–509.
  • Keegan, L., G. Gill, and Μ. Ptashne. 1986. Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. Science 231:699–704.
  • Kurjan, J., J. P. Hirsch, and C. Dietzel. 1991. Mutations in the guanine nucleotide-binding domains of a yeast Gα protein confer a constitutive or uninducible state to the pheromone response pathway. Genes Dev. 5:475–483.
  • Ma, J., and Μ. Ptashne. 1987. Deletion analysis of GAL4 defines two transcriptional activating segments. Cell 48:847–853.
  • Ma, J., and Μ. Ptashne. 1987. The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80. Cell 50:137–142.
  • MacKay, V. L., and T. R. Manney. 1974. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of non-mating mutants. Genetics 76:273–288.
  • Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Mead, D. A., E. Szczesna-Skorpa, and B. Kemper. 1986. Singlestranded DNA “blue” T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng. 1:67–74.
  • Miyajima, I., M. Nakafuku, N. Nakayama, C. Brenner, A. Miyajima, K. Kaibuchi, K. Arai, Y. Kaziro, and K. Matsumoto. 1987. GPA1, a haploid essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 50:1011–1019.
  • Munder, T., and P. Furst. 1992. The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo. Mol. Cell. Biol. 12:2091–2099.
  • Nakayama, N., A. Miyajima, and K. Arai. 1985. Nucleotide sequences of STE2 and STE3, cell type specific sterile genes from Saccharomyces cerevisiae. EMBO J. 4:2643–2648.
  • Neer, E. J., and D. E. Clapham. 1988. Roles of G protein subunits in transmembrane signalling. Nature (London) 333:129–134.
  • Nomoto, S., N. Nakayama, K.-I. Arai, and K. Matsumoto. 1990. Regulation of the yeast pheromone response pathway by G protein subunits. EMBO J. 9:691–696.
  • Orr-Weaver, T. C., J. Szostak, and R. J. Rothstein. 1981. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. USA 78:6354–6358.
  • Pronin, A. N., and N. Gautam. 1992. Interaction between G-protein β and γ subunit types is selective. Proc. Natl. Acad. Sci. USA 89:6220–6224.
  • Rothstein, R. J. 1983. One-step gene replacement in yeast. Methods Enzymol. 101:202–211.
  • Saiki, R. J., D. H. Gelfand, S. Stoffel, S. J. Scharf, R. Higuchi, G. T. Horn, K. B. Mullis, and H. A. Erlich. 1988. Primer- directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491.
  • Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467.
  • Schmidt, C. J., and E. J. Neer. 1991. In vitro synthesis of G protein βγ dimers. J. Biol. Chem. 266:4538–4544.
  • Sherman, F., G. R. Fink, and J. B. Hicks. 1982. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Silver, P. A., L. P. Keegan, and Μ. Ptashne. 1984. Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization. Proc. Natl. Acad. Sci. USA 81:5951–5955.
  • Simon, Μ. I., Μ. P. Strathmann, and N. Gautam. 1991. Diversity of G proteins in signal transduction. Science 252:802–808.
  • Simonds, W. F., J. E. Butrynski, N. Gautam, C. G. Unson, and A. Μ. Spiegel. 1991. G-protein βγ dimers. J. Biol. Chem. 266:5363–5366.
  • Stevenson, B. J., N. Rhodes, B. Errede, and G. F. Sprague, Jr. 1992. Constitutive mutants of the protein kinase STEll activate the yeast pheromone response pathway in the absence of the G protein. Genes Dev. 6:1293–1304.
  • Stone, D. E., and S. I. Reed. 1990. G protein mutations that alter the pheromone response in Saccharomyces cerevisiae. Mol. Cell. Biol. 10:4439–4446.
  • Stryer, L., and H. R. Bourne. 1986. G proteins: a family of signal transducers. Ann. Rev. Cell Biol. 2:391–419.
  • Vernet, T., D. Dignard, and D. Y. Thomas. 1987. A family of yeast expression vectors containing the phage f1 origin of replication. Gene 52:225–233.
  • Whiteway, M., L. Hougan, D. Dignard, L. Bell, G. Saari, F. Grant, P. O’Hara, V. L. MacKay, and D. Y. Thomas. 1988. Function of the STE4 and STE18 genes in mating pheromone signal transduction in Saccharomyces cerevisiae. Cold Spring Harbor Symp. Quant. Biol. 53:585–590.
  • Whiteway, M., L. Hougan, and D. Y. Thomas. 1990. Overexpression of the STE4 gene leads to mating response in haploid Saccharomyces cerevisiae. Mol. Cell. Biol. 10:217–222.
  • Whiteway, Μ. S., L. Hougan, D. Dignard, D. Y. Thomas, L. Bell, G. C. Saari, F. J. Grant, P. O'Hara, and V. L. MacKay. 1989. The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein. Cell 56:467–477.
  • Whiteway, Μ. S., L. Hougan, and D. Y. Thomas. 1988. Expression of MFα1 in MATa cells supersensitive to α-factor leads to self-arrest. Mol. Gen. Genet. 214:85–88.

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