A Drosophila Homolog of Bovine smg p25a GDP Dissociation Inhibitor Undergoes a Shift in Isoelectric Point in the Developmental Mutant quartet

REFERENCES

• Abo, A., E. Pick, A. Hall, N. Totty, C. G. Teahan, and A. W. Segal. 1991. Activation of the NADPH oxidase involves the small GTPase p21rac1. Nature (London) 353:668–670.
   PubMed Web of Science ®Google Scholar
• Bailly, E., Μ. McCaffrey, N. Touchot, A. Zahraoui, B. Goud, and Μ. Bornens. 1991. Phosphorylation of two small GTPases of the Rab family by p34cdc2. Nature (London) 350:715–718.
   PubMed Web of Science ®Google Scholar
• Balch, W. E. 1990. Small GTP-binding proteins in vesicular transport. Trends Biochem. Sci. 15:473–476.
   PubMed Web of Science ®Google Scholar
• Biggs, J., E. Hersperger, P. S. Steeg, L. A. Liotta, and A. Shearn. 1990. A Drosophila gene that is homologous to a mammalian gene associated with tumor metastasis codes for a nucleoside diphosphate kinase. Cell 63:933–940.
   PubMed Web of Science ®Google Scholar
• Biggs, J., N. Tripoulas, E. Hersperger, C. Dearolf, and A. Shearn. 1988. Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila. Genes Dev. 2:1333–1343.
   PubMedGoogle Scholar
• Borner, C., I. Filipuzzi, Μ. Wartmann, U. Eppenberger, and D. Fabbro. 1989. Biosynthesis and post-translational modifications of protein kinase C in human breast cancer cells. J. Biol. Chem. 264:13902–13909.
   PubMed Web of Science ®Google Scholar
• Bourne, H. R., D. A. Sanders, and F. McCormick. 1990. The GTPase superfamily: a conserved switch for diverse cell functions. Nature (London) 348:125–132.
   PubMed Web of Science ®Google Scholar
• Bourne, H. R., D. A. Sanders, and F. McCormick. 1991. The GTPase superfamily: conserved structure and molecular mechanism. Nature (London) 349:117–127.
   PubMed Web of Science ®Google Scholar
• Bradford, Μ. Μ. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 86:142–146.
   Google Scholar
• Charbonneau, H. 1989. Strategies for obtaining partial amino acid sequence data from small quantities (<5nmol) of pure or partially pure protein, p. 24–30. In P. T. Matsudaira (ed.), A practical guide to protein and peptide purification for microsequencing. Academic Press, Inc., New York.
   Google Scholar
• Cheney, C. Μ. Unpublished data.
   Google Scholar
• Cheney, C. Μ., and T. J. Lang. 1986. Defects in protein modification precede developmental defects in I(3)c21RRW630, a temperature-sensitive Drosophila developmental mutation. Dev. Biol. 114:34–41.
   PubMedGoogle Scholar
• Cheney, C. M., and T. J. Lang. 1988. Developmental and protein modification defects caused by mutations in the Drosophila gene, I(3)c21R. Dev. Biol. 130:551–557.
   PubMedGoogle Scholar
• Cheney, C. Μ., K. G. Miller, T. J. Lang, and A. Shearn. 1984. Specific protein modifications are altered in a temperature-sensitive Drosophila developmental mutant. Proc. Natl. Acad. Sci. USA 81:6422–6426.
   PubMedGoogle Scholar
• Cheney, C. Μ., and A. Shearn. 1983. Developmental regulation of Drosophila imaginal disc proteins: synthesis of heat shock proteins under non-heat-shock conditions. Dev. Biol. 95:325–330.
   PubMed Web of Science ®Google Scholar
• Cleveland, D. W., S. G. Fischer, Μ. W. Kirschner, and U. K. Laemmli. 1977. Peptide mapping by limited proteolysis in so-dium dodecyl sulfate and analysis by gel electrophoresis. J. Biol. Chem. 252:1102–1106.
   PubMed Web of Science ®Google Scholar
• Dearolf, C. R., E. Hersperger, and A. Shearn. 1988. Developmental consequences of awdb3, a cell-autonomous lethal mutation of Drosophila induced by hybrid dysgenesis. Dev. Biol. 129:159–168.
   PubMed Web of Science ®Google Scholar
• Feinberg, A. P., and B. Vogelstein. 1984. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137:266–267.
   PubMed Web of Science ®Google Scholar
• Fukumoto, Y., K. Kaibucki, Y. Hori, H. Fujioka, S. Araki, T. Ueda, A. Kikuchi, and Y. Takai. 1990. Molecular cloning and characterization of a novel type of regulatory protein (GDI) for the rho proteins, ras p21-like small GTP binding proteins. Oncogene 5:1321–1328.
   PubMed Web of Science ®Google Scholar
• Fyrberg, E. A., and J. J. Donady. 1979. Actin heterogeneity in primary embryonic culture cells from Drosophila melanogaster. Dev. Biol. 68:487–502.
   PubMedGoogle Scholar
• Hall, A. 1990. The cellular functions of small GTPases. Science 249:635–640.
   PubMed Web of Science ®Google Scholar
• Hancock, J. F., K. Cadwallader, and C. J. Marshall. 1991. Methylation and proteolysis are essential for efficient membrane binding of prenylated p21K-ras. EMBO J. 10:641–646.
   PubMed Web of Science ®Google Scholar
• Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Johnson-Schlitz, D., and J. K. Lim. 1987. Cytogenetics of Notch mutations arising in the unstable X chromosome Uc of Drosophila melanogaster. Genetics 115:701–709.
   PubMedGoogle Scholar
• Johnston, P. A., B. T. Archer III, K. Robinson, G. A. Mignery, R. Joan, and T. C. Sudhof. 1991. rab3A attachment to the synaptic vesicle membrane mediated by a conserved polyisoprenylated carboxy-terminal sequence. Neuron 7:101–109.
   PubMedGoogle Scholar
• Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685.
   PubMed Web of Science ®Google Scholar
• Lang, T. J., J. E. Zahner, and C. Μ. Cheney. Unpublished data.
   Google Scholar
• Langer-Safer, P. R., Μ. Levine, and D. C. Ward. 1982. Immunological method for mapping genes on Drosophila polytene chromosomes. Proc. Natl. Acad. Sci. USA 79:4381–4385.
   PubMed Web of Science ®Google Scholar
• Matsui, Y., A. Kikuchi, S. Araki, Y. Hata, J. Kondo, Y. Teranishi, and Y. Takai. 1990. Molecular cloning and characterization of a novel type of regulatory protein (GDI) for smg p25A, a ras p21-like GTP-binding protein. Mol. Cell. Biol. 10:4116–4122.
   PubMedGoogle Scholar
• O’Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:4007–4021.
   PubMed Web of Science ®Google Scholar
• Pearson, W. R., and D. J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85:2444–2448.
   PubMed Web of Science ®Google Scholar
• Pentz, E. S. 1979. Mutational dissection of development: analysis of mutations of the c21R locus which cause abnormal imaginal disc development in Drosophila melanogaster. Ph.D. dissertation. The Johns Hopkins University, Baltimore, Md.
   Google Scholar
• Pentz, E. S., and A. Shearn. 1977. Analysis of the autonomy of imaginal disc defects in a small-disc mutant of Drosophila melanogaster. Dev. Biol. 70:149–170.
   Google Scholar
• Pollard, K. Μ., E. K. L Chan, B. J. Grant, K. F. Sullivan, E. Μ. Tan, and C. A. Glass. 1990. In vitro posttranslational modification of lamin B cloned from a human T-cell line. Mol. Cell. Biol. 10:2164–2175.
   PubMedGoogle Scholar
• Poodry, C. A. 1990. shibire, a neurogenic mutant of Drosophila. Dev. Biol. 138:464–472.
   PubMedGoogle Scholar
• Ruggieri, R., and F. McCormick. 1991. Ras and the awd couple. Nature (London) 353:390–391.
   PubMedGoogle Scholar
• Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467.
   PubMed Web of Science ®Google Scholar
• Sasaki, T., K. Kaibuchi, A. K. Kabcenell, P. J. Novick, and Y. Takai. 1991. A mammalian inhibitory GDP/GTP exchange protein (GDP dissociation inhibitor) for smg p25A is active on the yeast SEC4 protein. Mol. Cell. Biol. 11:2909–2912.
   PubMedGoogle Scholar
• Sasaki, T., A. Kikuchi, S. Araki, Y. Hata, Μ. Isomura, S. Kuroda, and Y. Takai. 1990. Purification and characterization from bovine brain cytosol of a protein that inhibits the dissociation of GDP from and the subsequent binding of GTP to smg p25A, a ras p21-like GTP-binding protein. J. Biol. Chem. 265:2333–2337.
   PubMed Web of Science ®Google Scholar
• Smith, D. B., and K. S. Johnson. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase. Gene 67:31–40.
   PubMed Web of Science ®Google Scholar
• Southern, E. Μ. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.
   PubMed Web of Science ®Google Scholar
• Takai, Y., K. Kaibuchi, A. Kikuchi, and Μ. Kawata. 1992. Small GTP-binding proteins. Int. Rev. Cytol. 133:187–230.
   PubMedGoogle Scholar
• Teng, D. H. F., C. Μ. Engele, and T. R. Venkatesh. 1991. A product of the prune locus of Drosophila is similar to mammalian GTPase-activating protein. Nature (London) 353:437–440.
   PubMedGoogle Scholar
• Ueda, T., A. Kikuchi, N. Ohga, J. Yamamoto, and Y. Takai. 1990. Purification and characterization from bovine brain cytosol of a novel regulatory protein inhibiting the dissociation of GDP from and the subsequent binding of GTP to rhoB p20, a ras p21-like GTP-binding protein. J. Biol. Chem. 265:9373–9380.
   PubMed Web of Science ®Google Scholar
• Ueda, T., Y. Takeyama, T. Ohmori, H. Ohyanagi, Y. Satoh, and Y. Takai. 1991. Purification and characterization from rat liver cytosol of a GDP dissociation inhibitor (GDI) for liver 24K G, a ras p21-like GTP-binding protein, with properties similar to those of smg p25a GDI. Biochemistry 30:909–917.
   PubMedGoogle Scholar
• Wang, L. L., and J. A. Spudich. 1984. A 45,000-mol-wt protein from unfertilized sea urchin eggs severs actin filaments in a calcium-dependent manner and increases the steady-state concentration of nonfilamentous actin. J. Cell Biol. 99:844–851.
   PubMedGoogle Scholar
• Zahner, J. E., and C. Μ. Cheney. 1990. quartet: a Drosophila developmental mutation affecting chromosome separation in mitosis. Dev. Genet. 11:27–40.
   PubMedGoogle Scholar